Abstract:
PROBLEM TO BE SOLVED: To provide an automatic analyzer without increasing a size of the analyzer and without complicating the analyzer, which is capable of detecting a measuring object with high sensitivity by correcting variations of light quantity data due to thermal deformation in an optical system caused by temperature variations in the analyzer.SOLUTION: Scattered light from a measuring object through a light-receiving window 43 is received by a detector 45a for scattered light at +θ angle and a detector 45b for scattered light at -θ angle, which are symmetrically arranged at equal angles and at equal intervals in a vertical direction about an optical axis as a center. A light source 40 is fixed by a light source holder 46 (a base member in which the light source is arranged), and the detectors 45a and 45b are arranged in a detector holder 47 (a base member in which each of the detectors is arranged) to be fixed. Thus comparing values of light quantity data from each of the detectors 45a and 45b allows a drift of the light quantity data due to thermal deformation in an optical system to be corrected.
Abstract:
PROBLEM TO BE SOLVED: To provide a correction method of a fluorescence sensor 10 having high detection accuracy.SOLUTION: In the correction method of the fluorescence sensor in an embodiment, the fluorescence sensor 10 is used which includes: an LED element 12 which generates excitation light E; an indicator layer 16 which generates fluorescence F; and a PD element 13 which outputs a detection signal obtained by superimposing an excitation light detection signal caused by the excitation light E on a fluorescence detection signal caused by the fluorescence F, and which has a temperature detection function and a temperature adjusting function. This correction method includes: a first detection signal acquiring step of acquiring a first detection signal at a first temperature; a second detection signal acquiring step of acquiring, at a second temperature, a second detection signal when the amount of analyte is the same as that in the first detection signal acquiring step; a correction coefficient calculation step of calculating a correction coefficient for calculating a fluorescence detection signal based on the first detection signal and the second detection signal; and a correction step of correcting the detection signal after that, using the correction coefficient and the temperature detection signal.