摘要:
PROBLEM TO BE SOLVED: To provide a method for producing protoplasts of cassava or closely related species, which protoplasts are capable of regeneration into plants. SOLUTION: The method comprises producing friable embryogenic callus from explants of cassava or closely related species and isolating protoplasts from the friable embryogenic callus. The invention also concerns protoplasts obtainable by the method. The invention further relates to a method for transforming such a protoplast of cassava or closely related species, and transformed protoplasts obtainable thereby. In addition, the invention concerns a method for regenerating plants from these protoplasts and a cassava plant or closely related species obtainable thereby. COPYRIGHT: (C)2011,JPO&INPIT
摘要:
PROBLEM TO BE SOLVED: To provide nucleic acid molecules encoding a starch granule-bound protein from rice as well as methods and recombinant DNA molecules for the production of transgenic plant cells and plants synthesizing a modified starch. SOLUTION: There are provided: the nucleic acid molecules encoding an R1-protein; their respective complementary strands; an R1-gene naturally present in rice; and the methods for the production of plants, which includes a step of introducing polynucleotides encoding the R1-protein into plant cells and a step of producing plants from the cells transformed thereby. COPYRIGHT: (C)2011,JPO&INPIT
摘要:
This invention provides a DNA sequence capable of encoding an enzyme protein which is the alpha -subunit of the first isozyme of rice anthranilate synthase (ASA); and a DNA capable of encoding an enzyme protein which is the alpha -subunit of the second isozyme of ASA. Transformed plants having a high tryptophan content can be regenerated by culturing the plant cells having introduced therein such a recombinant vector which contains, at a position downstream of a promoter, an inserted DNA fragment carrying the above DNA sequence. This invention also provides a DNA having a promoter activity for the rice ASA gene, which DNA can be used as a promoter for introducing a useful exogenous gene into rice or other plants and expressing the gene therein.
摘要:
New gene (I) encodes 5-enol-pyruvylshikimate-3-phosphate synthase (EPSPS) that contains at least the Thr102Ile mutation. Also new are: (1) EPSPS mutant protein (II) with this mutation; (2) chimeric gene contg. (I) plus heterologous 5' and 3' regulatory elements; (3) vectors for plant transformation contg. at least one chimeric gene as in (2); and (4) plant cells (and plants derived from them) contg. the chimeric gene. The gene is pref. of bacterial or plant origin, in particular it is from Salmonella typhimurium or maize. The gene may further comprise at least one additional mutation, esp. resulting in the substitution Pro106Ser or Gly101Ala. The chimeric gene includes a promoter from a plant virus or plant, e.g. alpha -tubulin, histone, etc. Opt. it also includes a sequence encoding a transit peptide.
摘要:
PROBLEM TO BE SOLVED: To provide methods and compositions for the expression of transgenes in monocot plants including maize. SOLUTION: Gene silencing is avoided by use of monocot-homeologous sequences from plants of the genus Coix for transformation. Included in these transgene sequences are Coix promoters, enhancers, coding sequences and terminators. Suitable alternatives to maize-derived transgenes are desirable for expression in maize in that homology-based gene silencing can limit or effectively eliminate transgene expression. COPYRIGHT: (C)2011,JPO&INPIT
摘要:
PROBLEM TO BE SOLVED: To provide methods for making statin, such as atorvastatin (LIPITOR (R)), rosuvastatin (CRESTOR (R)), fluvastatin (LESCOL (R)), related compounds, and their intermediates. SOLUTION: The invention provides novel aldolases, nucleic acids encoding them, e.g., nucleic acids of a gene sequence comprising a specific sequence, or a polypeptide comprising an amino acid sequence of a specific sequence or an enzymatically active fragment thereof, and uses of them. COPYRIGHT: (C)2011,JPO&INPIT
摘要:
PROBLEM TO BE SOLVED: To provide a new method for giving plants the resistance to a weed control agent. SOLUTION: There is provided a method for giving plants the resistance to the weed control agent, wherein the method includes a step for introducing a gene encoding proteins having characteristics of the following (1), (2) and (3) to plant cells and expressing: (1) specifically combining to materials relating to weed control action of the weed control agent; (2) substantially having no denaturation performance to materials, to which the protein specifically binds; and (3) substantially containing no framework region of a variable region in immunoglobulin. COPYRIGHT: (C)2011,JPO&INPIT