Method for producing single strand gene tag group containing transcription starting site
    1.
    发明专利
    Method for producing single strand gene tag group containing transcription starting site 审中-公开
    用于生产包含转录开始站点的单条基因标签组的方法

    公开(公告)号:JP2007028923A

    公开(公告)日:2007-02-08

    申请号:JP2005212960

    申请日:2005-07-22

    摘要: PROBLEM TO BE SOLVED: To provide a method for producing a single strand gene tag group reflective of a kind and a ratio of quantity of a base sequence group at the mRNA 5' terminal extracted from a eukaryotic cell, to provide a method for measuring an expression level of a gene in the eukaryotic cell including a process for hybridizing a solid phase on which a DNA or a RNA containing a transcription starting site is immobilized with the single strand gene tag group, and to provide a method for preparing a gene expression profile by integrating obtained information on gene expression. SOLUTION: This single strand gene tag group reflective of the kind and the ratio of the quantity of the base sequence at the mRNA 5' terminal is produced by combining a method disclosed in a 5'SAGE method with a procedure for forming a double-stranded DNA into a single strand. Thus, it is found that the single strand gene tag group is effective for recognizing expression of a gene which targets an expression starting site, as a result of conducting hybridization with a DNA chip by using the tag group as a sample. Further, utilization of the single strand gene tag group makes it possible to conduct comprehensive expression analysis of a gene which targets various transcription starting sites. COPYRIGHT: (C)2007,JPO&INPIT

    摘要翻译: 待解决的问题:提供一种反映从真核细胞提取的mRNA 5'末端的碱基序列组的种类和量的比例的单链基因标签组的制备方法,以提供一种方法 用于测量真核细胞中基因的表达水平,包括将含有转录起始位点的DNA或RNA固定在其上的固相与单链基因标签基团杂交的方法,以及提供制备 通过整合获得的基因表达信息来获得基因表达谱。 解决方案:通过将5'SAGE方法中公开的方法与形成一种或多种mRNA的方法相结合的方法来产生反映mRNA 5'末端的碱基序列的种类和量的比例的单链基因标签组 双链DNA变为单链。 因此,作为通过使用标签组作为样品与DNA芯片进行杂交的结果,发现单链基因标签组对于识别靶向表达起始位点的基因的表达是有效的。 此外,单链基因标签组的利用使得可以进行靶向各种转录起始位点的基因的全面表达分析。 版权所有(C)2007,JPO&INPIT

    ジスルフィド架橋形成型インビトロタンパク質合成方法

    公开(公告)号:JPWO2005105994A1

    公开(公告)日:2008-03-13

    申请号:JP2006512879

    申请日:2005-04-28

    CPC分类号: C12P21/02 C07K1/1133

    摘要: 本発明の課題は、ジスルフィド結合を持つタンパク質を、再構成タンパク質合成系で簡便且つ高効率に製造する方法を提供することにある。チオレドキシンレダクターゼ[EC1.6.4.5]及びグルタレドキシンレダクターゼ[EC1.6.4.2]を含む還元状態を維持する種々の酵素や基質が存在するために酸化還元状態の調製が難しい細胞抽出液又はその粗画分を用いることに代え、これら酸化還元状態に影響の与える酵素及び基質を除去し、精製した構成成分からなる再構成タンパク質合成系において、ジスルフィド及びチオールの間の酸化還元の平衡状態を人為的に調整した再構成タンパク質合成系を用いることにより、活性のあるタンパク質を効率的に合成できることを見出したものである。

    Method for acquiring gene tag
    8.
    发明专利
    Method for acquiring gene tag 有权
    获取基因标签的方法

    公开(公告)号:JP2005185269A

    公开(公告)日:2005-07-14

    申请号:JP2004006630

    申请日:2004-01-14

    CPC分类号: C12Q1/6855 C12N15/1096

    摘要: PROBLEM TO BE SOLVED: To provide a method for acquiring a gene tag and a method for analyzing the gene tag. SOLUTION: A method for generating 5' terminal base sequence of an mRNA as a tag is provided. The method of the invention includes a process for synthesizing cDNA using mRNA connecting a IIs linker containing the recognition sequence of a IIs type restriction enzyme as a template to CAP (catabolite gene activator protein) structure. The tag comprising 5' terminal base sequence is generated by acting the IIs restriction enzyme to the cDNA. The tag is generated from every mRNA without depending on the base sequence. A method for identifying the initial point of transcription and a primer for synthesizing the total length cDNA are provided depending on the information on the base sequence of the tag. COPYRIGHT: (C)2005,JPO&NCIPI

    摘要翻译: 待解决的问题:提供获取基因标签的方法和分析基因标签的方法。 提供了一种产生mRNA的5'末端碱基序列作为标签的方法。 本发明的方法包括使用将含有IIs型限制酶的识别序列的IIs连接体作为模板连接到CAP(分解代谢基因激活蛋白)结构的mRNA合成cDNA的方法。 包含5'末端碱基序列的标签是通过将IIs限制酶作用于cDNA产生的。 标签是从每个mRNA产生而不依赖于碱基序列。 根据关于标签的基本序列的信息,提供用于鉴定初始转录点的方法和用于合成全长cDNA的引物。 版权所有(C)2005,JPO&NCIPI