摘要:
PROBLEM TO BE SOLVED: To provide a method for producing a single strand gene tag group reflective of a kind and a ratio of quantity of a base sequence group at the mRNA 5' terminal extracted from a eukaryotic cell, to provide a method for measuring an expression level of a gene in the eukaryotic cell including a process for hybridizing a solid phase on which a DNA or a RNA containing a transcription starting site is immobilized with the single strand gene tag group, and to provide a method for preparing a gene expression profile by integrating obtained information on gene expression. SOLUTION: This single strand gene tag group reflective of the kind and the ratio of the quantity of the base sequence at the mRNA 5' terminal is produced by combining a method disclosed in a 5'SAGE method with a procedure for forming a double-stranded DNA into a single strand. Thus, it is found that the single strand gene tag group is effective for recognizing expression of a gene which targets an expression starting site, as a result of conducting hybridization with a DNA chip by using the tag group as a sample. Further, utilization of the single strand gene tag group makes it possible to conduct comprehensive expression analysis of a gene which targets various transcription starting sites. COPYRIGHT: (C)2007,JPO&INPIT
摘要:
PROBLEM TO BE SOLVED: To provide a highly safe recombinant virus having efficacy for prevention of the infection with and onset of SARS (serious acute respiratory syndrom); and to provide a vaccine for SARS coronavirus containing the recombinant virus. SOLUTION: The recombinant virus can express a SARS coronavirus gene. The vaccine for neutralizing the SARS coronavirus contains the recombinant virus. COPYRIGHT: (C)2006,JPO&NCIPI
摘要:
PROBLEM TO BE SOLVED: To provide a recombinant virus which is effective for preventing the development of hepatitis C infection and is very safe, and to provide vaccinia for hepatitis C virus containing the same. SOLUTION: The recombinant vaccinia virus can express a hepatitis C virus gene. The hepatitis C virus vaccine includes the recombinant virus. COPYRIGHT: (C)2010,JPO&INPIT
摘要:
The present invention provides methods for providing as tags the nucleotide sequences at the 5' end of mRNA. The method of the present invention comprises the step of synthesizing cDNA using, as a template, mRNA whose CAP structure is linked with a IIs linker having a type IIs endonuclease recognition sequence. Tags including the nucleotide sequence from the 5' end of mRNA are generated by reacting the type IIs endonuclease to cDNA. Tags can be generated from all mRNA, independently of their nucleotide sequences. Methods for identifying transcriptional start sites and primers for full-length cDNA synthesis are provided based on the nucleotide sequence information of tags of the present invention.
摘要:
PROBLEM TO BE SOLVED: To provide a method for acquiring a gene tag and a method for analyzing the gene tag. SOLUTION: A method for generating 5' terminal base sequence of an mRNA as a tag is provided. The method of the invention includes a process for synthesizing cDNA using mRNA connecting a IIs linker containing the recognition sequence of a IIs type restriction enzyme as a template to CAP (catabolite gene activator protein) structure. The tag comprising 5' terminal base sequence is generated by acting the IIs restriction enzyme to the cDNA. The tag is generated from every mRNA without depending on the base sequence. A method for identifying the initial point of transcription and a primer for synthesizing the total length cDNA are provided depending on the information on the base sequence of the tag. COPYRIGHT: (C)2005,JPO&NCIPI