摘要:
PROBLEM TO BE SOLVED: To identify one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. SOLUTION: The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between a first oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide hybridized to the solid support. The ligation phase can be preceded by an amplification process. The present invention also relates to a kit for practicing this method, a method of forming arrays on solid supports, and the supports themselves. COPYRIGHT: (C)2008,JPO&INPIT
摘要:
PROBLEM TO BE SOLVED: To provide a silanation composition useful for silanating of a surface containing silica or other silicon oxide, and having an enough stability against hydrolysis. SOLUTION: The silanation composition contains a mixture of at least two sorts of silanation agents, at least one sort of them contains a functional group capable of supporting polymer synthesis, e.g. bis(2-hydroxyethyl)-3-aminopropyl triethoxysilane, and at least one sort of them does not contain a functional group capable of supporting polymer synthesis, e.g. 1,2-bis(trimethoxysilyl)ethane. COPYRIGHT: (C)2008,JPO&INPIT
摘要:
PROBLEM TO BE SOLVED: To provide a device for printing biomolecules through the utilization of electrohydrodynamic phenomena. SOLUTION: The printing system is provided with a first needle-like electric field forming an electrode made of an electrically conductive material, longitudinally arranged in the vertical direction, and having both a housing part of a biomolecular solution and a discharge opening, formed in the lower end part the housing part; a substrate, arranged below the electrode and having a target part to which the biomolecular solution discharged from the discharge opening of the electrode, is dropped and adheres; a second electric field forming electrode, arranged below the electrode and made of an electrically conductive material; and a voltage impressing device electrically connected to the first electrode and the second electrode for impressing a voltage on the first and second electrodes, in such a way as to form an electric field around the biomolecular solution suspended from the discharge opening, generating electric force on the side of the biomolecular solution from the periphery of the biomolecular solution by interactions among an electric field distribution, the biomolecular solution having a free interface, and the gradient of the dielectric constant of the atmosphere, and dropping the biomolecular solution to the target part of the substrate. COPYRIGHT: (C)2007,JPO&INPIT
摘要:
A modified solid carrier composed of a solid carrier and a group of compounds having a vinylsulfonyl group which are fixed to the solid carrier with non-covalent bonding is favorably employed for preparing a device for detection of oligonucleotide or polynucleotide.
摘要:
PROBLEM TO BE SOLVED: To provide a manufacturing method of a PNA ZIP code chip using a ZIP code system for bonding a PNA probe onto a substrate modified with amine group, using an epoxy compound as a linker. SOLUTION: The manufacturing method of a PNA chip, using a ZIP code system comprises (a) a process of fixing the PNA ZIP code probe onto the substrate modified with amine group, by dropping an immobilized sample containing dimethyl sulfoxide (DMSO), PNA ZIP code probe, and epoxy compound, and (b) a process of cleaning with SDS solution, the substrate on which the PNA ZIP code probe has been immobilized and then drying it. COPYRIGHT: (C)2006,JPO&NCIPI
摘要:
PROBLEM TO BE SOLVED: To provide a composition of a photoacid generator monomer, a substrate coated by the composition, a method using the composition for synthesizing a compound on the substrate and a microarray manufactured by the method. SOLUTION: In the method, a first molecular layer having a protecting group which is unstable in acid, is made to bond to the surface of a solid substrate, and a composition layer of the monomer PAG is coated on the first molecular layer. Then, the layer of composition is exposed, and the protective group which is unstable in acid is removed from the first molecule corresponding to a portion being thermally treated and exposed, and the layer of composition of the monomer PAG is removed by carrying out cleaning. Next, a second molecule is made to bond to the activated first molecule. COPYRIGHT: (C)2005,JPO&NCIPI