公开(公告)号：US20110180725A1

公开(公告)日：2011-07-28

申请号：US13080274

申请日：2011-04-05

申请人： Kenichi Uchiyama ,  Shingo Kasai ,  Ikuo Uematsu ,  Tomohiro Takase ,  Kayoko Oomiya ,  Ichiro Tono ,  Tetsuo Sekino ,  Seiichirou Ishioka ,  Takashi Kawamura

发明人： Kenichi Uchiyama ,  Shingo Kasai ,  Ikuo Uematsu ,  Tomohiro Takase ,  Kayoko Oomiya ,  Ichiro Tono ,  Tetsuo Sekino ,  Seiichirou Ishioka ,  Takashi Kawamura

IPC分类号： G01J1/58

CPC分类号： G01N21/6428 ,  G01N21/07 ,  G01N21/645

摘要： A fluorometric apparatus capable of configuring to have a small size and operability requiring a simple and easy operation of centrifuging a specimen in a container a rotation center of which is detachably supported by a rotation axis (not shown), exciting the specimen by means of an N2 laser apparatus and an excitation light irradiator for guiding the laser light emitted from the N2 laser apparatus, and receiving fluorescence having two types of wavelengths emitted from antibodies labeled with fluorescent dyes in the specimen in a region separate from the region in which the excitation light is radiated by a predetermined angle by using a photomultiplier tube, obtaining a ratio of light intensity for each wavelength, comparing the obtained ratio with a standard value, and estimating a target protein concentration.

摘要翻译： 一种能够构造成具有小尺寸和可操作性的荧光测定装置，其需要简单且容易地将样品离心在容器中，旋转中心由旋转轴线（未示出）可拆卸地支撑，借助于 N2激光装置和激发光照射器，用于引导从N2激光装置发射的激光，并且在与激发光的区域分开的区域中接收在样品中的荧光染料标记的抗体的两种类型的波长的荧光 通过使用光电倍增管照射预定角度，获得每个波长的光强比，将获得的比值与标准值进行比较，并估计目标蛋白质浓度。

公开(公告)号：US20070219436A1

公开(公告)日：2007-09-20

申请号：US11538230

申请日：2006-10-03

申请人： Tomohiro Takase ,  Isao Nawata ,  Ikuo Uematsu ,  Kayoko Oomiya

发明人： Tomohiro Takase ,  Isao Nawata ,  Ikuo Uematsu ,  Kayoko Oomiya

IPC分类号： A61B5/00

CPC分类号： A61B5/14532

摘要： It is an object to provide a measuring apparatus and method which can easily measure an amount of a biological component such as glucose without requiring an ordinary person who is to be measured to have special knowledge and techniques. A measuring apparatus according to an embodiment of the present invention uses a biological component sensor including a plate-shaped sensor chip 112 having a biological component sensing function and a sensor chip holding frame 111 for surrounding and holding the sensor chip 112, and the biological component sensor is disposed in a measuring apparatus main body housing 110. A fixed click-shaped engaging member 118 and a movable click-shaped engaging member 119 are disposed on a sensor chip support table 124 fixed to the measuring apparatus main body housing 110, and they fix and support the sensor chip holding frame 111 and the sensor chip can be thus replaced. The biological component sensor of the measuring apparatus is caused to come in contact with a forearm portion 100 of a human body and a biological component exuded from a contact part of the forearm portion is caused to come in contact with the sensor chip to change the physical or chemical surface property of the sensor chip, thereby measuring the change through an irradiation of an inspection light. Thus, a concentration of the biological component is calculated.

摘要翻译： 本发明的目的是提供一种可以容易地测量诸如葡萄糖等生物成分的量的测量装置和方法，而不需要测量的普通人具有特殊的知识和技术。 根据本发明的实施例的测量装置使用包括具有生物部件感测功能的板状传感器芯片112和用于围绕和保持传感器芯片112的传感器芯片保持框架111的生物部件传感器和生物部件 传感器设置在测量装置主体外壳110中。 在固定在测量装置本体壳体110上的传感器芯片支撑台124上设置有固定的点击状的卡合部件118和活动卡爪部件119，并且固定并支撑传感器芯片固定框架111和传感器 芯片可以这样替换。 使测量装置的生物部件传感器与人体的前臂部分100接触，并且使从前臂部分的接触部分渗出的生物成分与传感器芯片接触以改变物理 或化学表面性质，从而通过检查光的照射来测量变化。 因此，计算生物成分的浓度。

公开(公告)号：US20050084909A1

公开(公告)日：2005-04-21

申请号：US10928319

申请日：2004-08-30

申请人： Kenichi Uchiyama ,  Kayoko Oomiya ,  Isao Kishimoto ,  Masami Hirata ,  Hideo Eto ,  Ichiro Tono ,  Ikuo Uematsu

发明人： Kenichi Uchiyama ,  Kayoko Oomiya ,  Isao Kishimoto ,  Masami Hirata ,  Hideo Eto ,  Ichiro Tono ,  Ikuo Uematsu

IPC分类号： C12M1/34 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/543 ,  G01N33/58

CPC分类号： G01N33/581 ,  G01N33/54306

摘要： An antigen measuring device has an antibody chip. The antibody chip has a substrate. A pair of gratings are formed on the substrate. An antibody-fixed layer surrounded by a wall is formed between the gratings. A light emitting element emits a light beam toward the first grating. A light receiving element receives the light beam which propagates through the substrate under the antibody-fixed layer and is outputfrom the second grating.

摘要翻译： 抗原测定装置具有抗体芯片。 抗体芯片具有底物。 在基板上形成一对光栅。 在光栅之间形成由壁包围的抗体固定层。 发光元件朝向第一光栅发射光束。 光接收元件接收在抗体固定层下传播通过衬底并从第二光栅输出的光束。

公开(公告)号：US09274104B2

公开(公告)日：2016-03-01

申请号：US13436115

申请日：2012-03-30

申请人： Ichiro Tono ,  Shingo Kasai ,  Takaaki Wada ,  Isao Nawata ,  Masaaki Hirakawa ,  Tomohiro Takase ,  Kayoko Oomiya ,  Takeshi Yamauchi ,  Tadahiro Nakayama ,  Isamu Nitta

发明人： Ichiro Tono ,  Shingo Kasai ,  Takaaki Wada ,  Isao Nawata ,  Masaaki Hirakawa ,  Tomohiro Takase ,  Kayoko Oomiya ,  Takeshi Yamauchi ,  Tadahiro Nakayama ,  Isamu Nitta

IPC分类号： G01N15/06 ,  G01N27/00 ,  G01N33/543 ,  G01N33/58

CPC分类号： G01N33/54326 ,  G01N15/0656 ,  G01N33/5434 ,  G01N33/54373 ,  G01N33/585

摘要： According to one embodiment, a measuring system using an optical waveguide is provided. The measuring system has an optical waveguide, magnetic fine particles, a magnetic field applying unit, a light source and a light receiving element. The optical waveguide has a sensing area to which first substances having a property of specifically bonding to subject substances to be measured are fixed. Second substances having a property of specifically bonding to the subject substances are fixed to the magnetic fine particle. The magnetic field applying unit generates a magnetic field for moving the magnetic fine particles. The light source inputs a light into the optical waveguide. The light receiving element receives the light output from the optical waveguide.

摘要翻译： 根据一个实施例，提供了使用光波导的测量系统。 测量系统具有光波导，磁性微粒，磁场施加单元，光源和光接收元件。 光波导具有感测区域，第一物质具有特异性地结合待测物质的特性固定。 具有特异性地结合到被摄体物质的特性的第二物质被固定在磁性细颗粒上。 磁场施加单元产生用于移动磁性细颗粒的磁场。 光源将光输入到光波导中。 光接收元件接收从光波导输出的光。

公开(公告)号：US07738934B2

公开(公告)日：2010-06-15

申请号：US11538230

申请日：2006-10-03

申请人： Tomohiro Takase ,  Isao Nawata ,  Ikuo Uematsu ,  Kayoko Oomiya

发明人： Tomohiro Takase ,  Isao Nawata ,  Ikuo Uematsu ,  Kayoko Oomiya

IPC分类号： A61B5/145

CPC分类号： A61B5/14532

摘要： A measuring apparatus uses a biological component sensor including a plate-shaped sensor chip having a biological component sensing function and a sensor chip holding frame surrounding and holding the sensor chip. The biological component sensor is disposed in a measuring apparatus main body housing. A fixed engaging member and movable engaging member disposed on a sensor chip support table fixed to the measuring apparatus main body housing fix and support the sensor chip holding frame, and the sensor chip can be thus replaced. The biological component sensor contacts a forearm portion of a human body, and a biological component exuded from a contact part of the forearm portion is caused to contact the sensor chip to change a physical or chemical surface property of the sensor chip, thereby measuring the change through an irradiation of an inspection light to calculate concentration of the biological component.

摘要翻译： 测量装置使用包括具有生物成分感测功能的板状传感器芯片和围绕并保持传感器芯片的传感器芯片保持框架的生物体成分传感器。 生物体传感器配置在测量装置主体壳体内。 设置在传感器芯片支撑台上的固定接合构件和可移动接合构件，固定到测量装置主体外壳固定并支撑传感器芯片保持框架，并且可以更换传感器芯片。 生物成分传感器接触人体的前臂部分，并且从前臂部分的接触部分渗出的生物成分与传感器芯片接触以改变传感器芯片的物理或化学表面性质，从而测量变化 通过照射检查光来计算生物成分的浓度。

公开(公告)号：US20090124024A1

公开(公告)日：2009-05-14

申请号：US12266023

申请日：2008-11-06

申请人： Shingo Kasai ,  Ikuo Uematsu ,  Ichiro Tono ,  Tomohiro Takase ,  Isao Nawata ,  Kayoko Oomiya ,  Yuriko Oyama ,  Tsutomu Honjoh

发明人： Shingo Kasai ,  Ikuo Uematsu ,  Ichiro Tono ,  Tomohiro Takase ,  Isao Nawata ,  Kayoko Oomiya ,  Yuriko Oyama ,  Tsutomu Honjoh

IPC分类号： G01N33/543 ,  G01N21/00 ,  B05D5/06

CPC分类号： G01N21/75 ,  G01N21/552 ,  G01N21/7703 ,  G01N33/54313 ,  G01N33/54373 ,  G02B6/12007 ,  G02B6/124

摘要： An optical-waveguide sensor chip includes an optical waveguide having a first substance immobilized on the surface thereof, the first substance being specifically reactive with an analyte substance, and fine particles dispersed on the optical waveguide and having a second substance immobilized on the surface thereof, the second substance being specifically reactive with the analyte substance.

摘要翻译： 光波导传感器芯片包括具有固定在其表面上的第一物质的光波导，第一物质与分析物质特异性反应，以及分散在光波导上并且固定在其表面上的第二物质的细颗粒， 第二物质与分析物质特异性反应。

公开(公告)号：US20080020454A1

公开(公告)日：2008-01-24

申请号：US11780033

申请日：2007-07-19

申请人： Ikuo Uematsu ,  Masaaki Hirakawa ,  Kayoko Oomiya

发明人： Ikuo Uematsu ,  Masaaki Hirakawa ,  Kayoko Oomiya

IPC分类号： C12M1/00

CPC分类号： C12Q1/002 ,  C12Q1/006 ,  G01N21/552 ,  G01N21/78

摘要： An optical glucose sensor chip includes a substrate, a pair of optical elements formed on a surface of the substrate for introducing light into the substrate and for emitting the light from the substrate, and a glucose sensing membrane formed on the surface of the substrate at a position between the optical elements. The sensing membrane includes a color reagent substrate, a first enzyme which oxidizes or reduces glucose, a second enzyme that generates a material which makes the color reagent substrate exhibit color by a reaction with a product obtained by oxidation or reduction of glucose, a nonionic cellulose derivative, and an ionic polymer into which a buffer is incorporated. At least one of the first and second enzymes is coated with the ionic polymer, and the color reagent substrate. The first and second enzymes, the buffer and the ionic polymer are supported by the nonionic cellulose derivative.

摘要翻译： 光学葡萄糖传感器芯片包括：基板，形成在基板的表面上的一对光学元件，用于将光引入基板并从基板发射光;以及葡萄糖感测膜，其形成在基板的表面上 光学元件之间的位置。 检测膜包括着色试剂底物，氧化或降低葡萄糖的第一种酶，产生通过与通过氧化或还原葡萄糖获得的产物的反应而使着色试剂底物显色的第二种酶，非离子型纤维素 衍生物和其中加入缓冲剂的离子聚合物。 第一和第二酶中的至少一种用离子聚合物和着色剂底物涂覆。 第一和第二酶，缓冲液和离子聚合物由非离子纤维素衍生物负载。

公开(公告)号：US20080003668A1

公开(公告)日：2008-01-03

申请号：US11850053

申请日：2007-09-05

申请人： Kenichi UCHIYAMA ,  Shingo KASAI ,  Ikuo UEMATSU ,  Tomohiro TAKASE ,  Kayoko OOMIYA ,  Ichiro TONO ,  Tetsuo SEKINO ,  Seiichirou ISHIOKA ,  Takashi KAWAMURA

发明人： Kenichi UCHIYAMA ,  Shingo KASAI ,  Ikuo UEMATSU ,  Tomohiro TAKASE ,  Kayoko OOMIYA ,  Ichiro TONO ,  Tetsuo SEKINO ,  Seiichirou ISHIOKA ,  Takashi KAWAMURA

IPC分类号： C12M1/34 ,  G01J3/42

CPC分类号： G01N21/6428 ,  G01N21/07 ,  G01N21/645

摘要： A fluorometric apparatus capable of configuring to have a small size and operability requiring a simple and easy operation of centrifuging a specimen in a container a rotation center of which is detachably supported by a rotation axis (not shown), exciting the specimen by means of an N2 laser apparatus and an excitation light irradiator for guiding the laser light emitted from the N2 laser apparatus, and receiving fluorescence having two types of wavelengths emitted from antibodies labeled with fluorescent dyes in the specimen in a region separate from the region in which the excitation light is radiated by a predetermined angle by using a photomultiplier tube, obtaining a ratio of light intensity for each wavelength, comparing the obtained ratio with a standard value, and estimating a target protein concentration.

摘要翻译： 一种能够构造成具有小尺寸和可操作性的荧光测定装置，其需要简单且容易地将样品离心在容器中，旋转中心由旋转轴线（未示出）可拆卸地支撑，借助于 N 2激光装置和用于引导从N 2激光装置发射的激光的激发光照射器，以及接收从用荧光标记的抗体发射的具有两种类型的波长的荧光 通过使用光电倍增管将与激发光辐射的区域分开的区域中的试样中的染料，得到各波长的光强度比，将所得比值与标准值进行比较， 靶蛋白浓度。

公开(公告)号：US07498145B2

公开(公告)日：2009-03-03

申请号：US11363094

申请日：2006-02-28

申请人： Kenichi Uchiyama ,  Kayoko Oomiya ,  Isao Kishimoto ,  Masami Hirata ,  Hideo Eto ,  Ichiro Tono ,  Ikuo Uematsu ,  Shingo Kasai ,  Tomohiro Takase ,  Tsutomu Honjoh ,  Masanori Sugitani

发明人： Kenichi Uchiyama ,  Kayoko Oomiya ,  Isao Kishimoto ,  Masami Hirata ,  Hideo Eto ,  Ichiro Tono ,  Ikuo Uematsu ,  Shingo Kasai ,  Tomohiro Takase ,  Tsutomu Honjoh ,  Masanori Sugitani

IPC分类号： G01N33/53 ,  G01N33/551 ,  G01N33/552 ,  G01N33/58

CPC分类号： G01N33/54373 ,  Y10S435/808 ,  Y10S436/805

摘要： The method for measuring the concentration of the measuring object uses a sensor chip comprising an optical waveguide layer and an antibody immobilized layer formed on the surface of the optical waveguide layer, which comprises immobilizing the measuring object and an enzyme-labeled antibody labeled with a labeling enzyme on the antibody immobilized layer of the sensor chip having an immobilized antibody, producing a color-developing and precipitating enzyme reaction product by allowing to react a coloring reagent with the labeling enzyme on the antibody immobilized layer to precipitate the enzyme reaction product on the antibody immobilized layer, allowing to totally reflect a light impinged on the sensor chip from the outside at an interface between the optical waveguide layer and the antibody immobilized layer, and observing to a physical value of the totally reflected light.

摘要翻译： 用于测量对象浓度的方法使用包括形成在光波导层表面上的光波导层和抗体固定层的传感器芯片，其包括固定测量对象和用​​标记的酶标记的抗体 酶在具有固定化抗体的传感器芯片的抗体固定化层上，通过使着色试剂与抗体固定化层上的标记酶反应，使酶反应产物沉淀在抗体上，产生显色和沉淀酶反应产物 使得能够在光波导层和抗体固定化层之间的界面处从外部全面反射入射到传感器芯片上的光，并观察全反射光的物理值。

公开(公告)号：US20070081758A1

公开(公告)日：2007-04-12

申请号：US11393771

申请日：2006-03-31

申请人： Ichiro Tono ,  Kayoko Oomiya ,  Tomohiro Takase ,  Ikuo Uematsu

发明人： Ichiro Tono ,  Kayoko Oomiya ,  Tomohiro Takase ,  Ikuo Uematsu

IPC分类号： G02B6/00

CPC分类号： G02B6/124 ,  G01N21/7703 ,  G01N21/78 ,  G01N2021/7709 ,  G01N2021/775 ,  G01N2021/7783

摘要： An optical waveguide type biochemical sensor chip includes a light beam transmittable substrate having at least a first optical element that allows light beam to be impinged to the inside and a second optical element that emits light beam from the inside, an optical waveguide layer that is formed on a main surface of the substrate on which at least one of the first and second optical elements is formed, has a thickness of 3 to 300 μm and is made of a polymer resin material having a higher refractive index than that of the substrate material, and a sensing membrane that is formed on the optical waveguide layer and creates a reaction product having the ability of absorbing the light beam or an evanescent wave of the light beam in response to an introduced specimen.