公开(公告)号：US20110180725A1

公开(公告)日：2011-07-28

申请号：US13080274

申请日：2011-04-05

申请人： Kenichi Uchiyama ,  Shingo Kasai ,  Ikuo Uematsu ,  Tomohiro Takase ,  Kayoko Oomiya ,  Ichiro Tono ,  Tetsuo Sekino ,  Seiichirou Ishioka ,  Takashi Kawamura

发明人： Kenichi Uchiyama ,  Shingo Kasai ,  Ikuo Uematsu ,  Tomohiro Takase ,  Kayoko Oomiya ,  Ichiro Tono ,  Tetsuo Sekino ,  Seiichirou Ishioka ,  Takashi Kawamura

IPC分类号： G01J1/58

CPC分类号： G01N21/6428 ,  G01N21/07 ,  G01N21/645

摘要： A fluorometric apparatus capable of configuring to have a small size and operability requiring a simple and easy operation of centrifuging a specimen in a container a rotation center of which is detachably supported by a rotation axis (not shown), exciting the specimen by means of an N2 laser apparatus and an excitation light irradiator for guiding the laser light emitted from the N2 laser apparatus, and receiving fluorescence having two types of wavelengths emitted from antibodies labeled with fluorescent dyes in the specimen in a region separate from the region in which the excitation light is radiated by a predetermined angle by using a photomultiplier tube, obtaining a ratio of light intensity for each wavelength, comparing the obtained ratio with a standard value, and estimating a target protein concentration.

摘要翻译： 一种能够构造成具有小尺寸和可操作性的荧光测定装置，其需要简单且容易地将样品离心在容器中，旋转中心由旋转轴线（未示出）可拆卸地支撑，借助于 N2激光装置和激发光照射器，用于引导从N2激光装置发射的激光，并且在与激发光的区域分开的区域中接收在样品中的荧光染料标记的抗体的两种类型的波长的荧光 通过使用光电倍增管照射预定角度，获得每个波长的光强比，将获得的比值与标准值进行比较，并估计目标蛋白质浓度。

公开(公告)号：US07860354B2

公开(公告)日：2010-12-28

申请号：US12265991

申请日：2008-11-06

申请人： Ikuo Uematsu ,  Tomohiro Takase ,  Ichiro Tono ,  Shingo Kasai

发明人： Ikuo Uematsu ,  Tomohiro Takase ,  Ichiro Tono ,  Shingo Kasai

IPC分类号： G02B6/00 ,  G02B6/34 ,  G01J3/00 ,  G01N33/28

CPC分类号： G01N21/7703 ,  G01D5/38 ,  G01N2021/7709 ,  G01N2021/7776 ,  G01N2021/7786 ,  G02B6/34

摘要： An optical sensor (1) includes a sensor chip member (2) having an optical waveguide layer (21), a combination of an incidence end grating (22a) and an output end grating (22b) spaced from each other, in contact with the optical waveguide layer (21), and a reaction reagent (23) provided on the optical waveguide layer (21) to detect as an optical change a quantity of measurement object interposed between the incidence end grating (22a) and the output end grating (22b), and a chamber member (3) to have, when the sensor chip member (2) is assembled, a facing surface (F) in position facing the optical waveguide layer (21), and a gap (I) defined between the optical waveguide layer (21) and the facing surface (F), the reaction reagent (23) being disposed in the gap (I).

摘要翻译： 光学传感器（1）包括具有光波导层（21）的传感器芯片部件（2），彼此间隔开的入射端光栅（22a）和输出端光栅（22b）的组合， 光波导层（21）和设置在光波导层（21）上的反应试剂（23），以检测插入在入射光栅（22a）和输出端光栅（22b）之间的测量对象的量作为光学变化 ）和室构件（3），当组装传感器芯片构件（2）时，在与光波导层（21）相对的位置处具有面对面（F）以及限定在光学元件 波导层（21）和相对表面（F），反应试剂（23）设置在间隙（I）中。

公开(公告)号：US20090116783A1

公开(公告)日：2009-05-07

申请号：US12265991

申请日：2008-11-06

申请人： Ikuo UEMATSU ,  Tomohiro Takase ,  Ichiro Tono ,  Shingo Kasai

发明人： Ikuo UEMATSU ,  Tomohiro Takase ,  Ichiro Tono ,  Shingo Kasai

IPC分类号： G02B6/00

CPC分类号： G01N21/7703 ,  G01D5/38 ,  G01N2021/7709 ,  G01N2021/7776 ,  G01N2021/7786 ,  G02B6/34

摘要： An optical sensor (1) includes a sensor chip member (2) having an optical waveguide layer (21), a combination of an incidence end grating (22a) and an output end grating (22b) spaced from each other, in contact with the optical waveguide layer (21), and a reaction reagent (23) provided on the optical waveguide layer (21) to detect as an optical change a quantity of measurement object interposed between the incidence end grating (22a) and the output end grating (22b), and a chamber member (3) to have, when the sensor chip member (2) is assembled, a facing surface (F) in position facing the optical waveguide layer (21), and a gap (I) defined between the optical waveguide layer (21) and the facing surface (F), the reaction reagent (23) being disposed in the gap (I).

摘要翻译： 光学传感器（1）包括具有光波导层（21）的传感器芯片部件（2），彼此间隔开的入射端光栅（22a）和输出端光栅（22b）的组合， 光波导层（21）和设置在光波导层（21）上的反应试剂（23），以检测插入在入射光栅（22a）和输出端光栅（22b）之间的测量对象的量作为光学变化 ）和室构件（3），当组装传感器芯片构件（2）时，在与光波导层（21）相对的位置处具有面对面（F）以及限定在光学元件 波导层（21）和相对表面（F），反应试剂（23）设置在间隙（I）中。

公开(公告)号：US20070219436A1

公开(公告)日：2007-09-20

申请号：US11538230

申请日：2006-10-03

申请人： Tomohiro Takase ,  Isao Nawata ,  Ikuo Uematsu ,  Kayoko Oomiya

发明人： Tomohiro Takase ,  Isao Nawata ,  Ikuo Uematsu ,  Kayoko Oomiya

IPC分类号： A61B5/00

CPC分类号： A61B5/14532

摘要： It is an object to provide a measuring apparatus and method which can easily measure an amount of a biological component such as glucose without requiring an ordinary person who is to be measured to have special knowledge and techniques. A measuring apparatus according to an embodiment of the present invention uses a biological component sensor including a plate-shaped sensor chip 112 having a biological component sensing function and a sensor chip holding frame 111 for surrounding and holding the sensor chip 112, and the biological component sensor is disposed in a measuring apparatus main body housing 110. A fixed click-shaped engaging member 118 and a movable click-shaped engaging member 119 are disposed on a sensor chip support table 124 fixed to the measuring apparatus main body housing 110, and they fix and support the sensor chip holding frame 111 and the sensor chip can be thus replaced. The biological component sensor of the measuring apparatus is caused to come in contact with a forearm portion 100 of a human body and a biological component exuded from a contact part of the forearm portion is caused to come in contact with the sensor chip to change the physical or chemical surface property of the sensor chip, thereby measuring the change through an irradiation of an inspection light. Thus, a concentration of the biological component is calculated.

摘要翻译： 本发明的目的是提供一种可以容易地测量诸如葡萄糖等生物成分的量的测量装置和方法，而不需要测量的普通人具有特殊的知识和技术。 根据本发明的实施例的测量装置使用包括具有生物部件感测功能的板状传感器芯片112和用于围绕和保持传感器芯片112的传感器芯片保持框架111的生物部件传感器和生物部件 传感器设置在测量装置主体外壳110中。 在固定在测量装置本体壳体110上的传感器芯片支撑台124上设置有固定的点击状的卡合部件118和活动卡爪部件119，并且固定并支撑传感器芯片固定框架111和传感器 芯片可以这样替换。 使测量装置的生物部件传感器与人体的前臂部分100接触，并且使从前臂部分的接触部分渗出的生物成分与传感器芯片接触以改变物理 或化学表面性质，从而通过检查光的照射来测量变化。 因此，计算生物成分的浓度。

公开(公告)号：US20050084909A1

公开(公告)日：2005-04-21

申请号：US10928319

申请日：2004-08-30

申请人： Kenichi Uchiyama ,  Kayoko Oomiya ,  Isao Kishimoto ,  Masami Hirata ,  Hideo Eto ,  Ichiro Tono ,  Ikuo Uematsu

发明人： Kenichi Uchiyama ,  Kayoko Oomiya ,  Isao Kishimoto ,  Masami Hirata ,  Hideo Eto ,  Ichiro Tono ,  Ikuo Uematsu

IPC分类号： C12M1/34 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/543 ,  G01N33/58

CPC分类号： G01N33/581 ,  G01N33/54306

摘要： An antigen measuring device has an antibody chip. The antibody chip has a substrate. A pair of gratings are formed on the substrate. An antibody-fixed layer surrounded by a wall is formed between the gratings. A light emitting element emits a light beam toward the first grating. A light receiving element receives the light beam which propagates through the substrate under the antibody-fixed layer and is outputfrom the second grating.

摘要翻译： 抗原测定装置具有抗体芯片。 抗体芯片具有底物。 在基板上形成一对光栅。 在光栅之间形成由壁包围的抗体固定层。 发光元件朝向第一光栅发射光束。 光接收元件接收在抗体固定层下传播通过衬底并从第二光栅输出的光束。

公开(公告)号：US07829327B2

公开(公告)日：2010-11-09

申请号：US12047670

申请日：2008-03-13

申请人： Ikuo Uematsu ,  Masatoshi Sakai ,  Shingo Kasai

发明人： Ikuo Uematsu ,  Masatoshi Sakai ,  Shingo Kasai

IPC分类号： G01N33/551 ,  G01N33/552

CPC分类号： G01N33/54373 ,  Y10S435/808 ,  Y10S436/805 ,  Y10S436/807

摘要： An optical waveguide type antibody chip includes a transparent substrate, an incident-side optical element and an emitting-side optical element placed at a distance from each other on a primary face of the substrate, a water repellent resin film formed on the primary face of the substrate including an optical waveguide layer formed between the optical elements, the water repellent resin film includes a reaction hole having exposed the optical waveguide layer on its bottom and a frame-shaped trench surrounding the reaction hole, a rectangular frame-shaped cell wall which is fixed in the trench of the water repellent resin film and which forms a cell capable of infusion and discharge of a specimen solution together with the reaction hole, and an antibody immobilization layer formed on the bottom of the reaction hole, the surface of the antibody immobilization layer being masked with at least a buffer agent and a salt.

摘要翻译： 光波导型抗体芯片包括在基板的主面上彼此相隔一定距离放置的透明基板，入射侧光学元件和发光侧光学元件，形成在基板的主面上的防水树脂膜 所述基板包括形成在所述光学元件之间的光波导层，所述防水树脂膜包括在其底部露出光波导层的反应孔和围绕所述反应孔的框状沟槽，矩形框状电池壁， 固定在防水树脂膜的沟槽中，并且形成能够与反应孔一起输注和排出样品溶液的电池，以及形成在反应孔底部的抗体固定层，抗体表面 固定层用至少缓冲剂和盐掩蔽。

公开(公告)号：US20100221408A1

公开(公告)日：2010-09-02

申请号：US12771343

申请日：2010-04-30

申请人： Ikuo UEMATSU ,  Masaaki Hirakawa ,  Kayoko Oomiya

发明人： Ikuo UEMATSU ,  Masaaki Hirakawa ,  Kayoko Oomiya

IPC分类号： B05D3/00

CPC分类号： C12Q1/002 ,  C12Q1/006 ,  G01N21/552 ,  G01N21/78

摘要： An optical glucose sensor chip includes a substrate, a pair of optical elements formed on a surface of the substrate for introducing light into the substrate and for emitting the light from the substrate, and a glucose sensing membrane formed on the surface of the substrate at a position between the optical elements. The sensing membrane includes a color reagent substrate, a first enzyme which oxidizes or reduces glucose, a second enzyme that generates a material which makes the color reagent substrate exhibit color by a reaction with a product obtained by oxidation or reduction of glucose, a nonionic cellulose derivative, and an ionic polymer into which a buffer is incorporated. At least one of the first and second enzymes is coated with the ionic polymer, and the color reagent substrate. The first and second enzymes, the buffer and the ionic polymer are supported by the nonionic cellulose derivative.

摘要翻译： 光学葡萄糖传感器芯片包括：基板，形成在基板的表面上的一对光学元件，用于将光引入基板并从基板发射光;以及葡萄糖感测膜，其形成在基板的表面上 光学元件之间的位置。 检测膜包括着色试剂底物，氧化或降低葡萄糖的第一种酶，产生通过与通过氧化或还原葡萄糖获得的产物的反应而使着色试剂底物显色的第二种酶，非离子型纤维素 衍生物和其中加入缓冲剂的离子聚合物。 第一和第二酶中的至少一种用离子聚合物和着色剂底物涂覆。 第一和第二酶，缓冲液和离子聚合物由非离子纤维素衍生物负载。

公开(公告)号：US07678567B2

公开(公告)日：2010-03-16

申请号：US10899266

申请日：2004-07-26

申请人： Ikuo Uematsu ,  Ichiro Tono ,  Kayoko Oomiya ,  Hideo Eto

发明人： Ikuo Uematsu ,  Ichiro Tono ,  Kayoko Oomiya ,  Hideo Eto

IPC分类号： C12M1/34 ,  C12M3/00

CPC分类号： C12Q1/00 ,  C12Q1/28 ,  C12Q1/54 ,  G01N21/7703 ,  G01N21/78

摘要： An optical biosensor has a total reflection plate so as to totally internally reflect and transmit an incident light, a first grating and a second grating disposed separately on the total reflection plate, and a sensing membrane that is containing an enzyme and a chromogenic reagent and is sandwiched by the first and second gratings on the total reflection plate.

摘要翻译： 光学生物传感器具有全反射板，以全反射和透射入射光，第一光栅和第二光栅分别设置在全反射板上，以及传感膜，其包含酶和显色试剂，并且是 夹在全反射板上的第一和第二光栅。

公开(公告)号：US07651864B2

公开(公告)日：2010-01-26

申请号：US11283861

申请日：2005-11-22

申请人： Shingo Kasai ,  Kayoko Oomiya ,  Ikuo Uematsu ,  Ichiro Tono

发明人： Shingo Kasai ,  Kayoko Oomiya ,  Ikuo Uematsu ,  Ichiro Tono

IPC分类号： G01N1/31 ,  G01N1/30 ,  G01N1/28 ,  G01N1/00

CPC分类号： G01N33/523 ,  C12Q1/54 ,  G01N31/22

摘要： A method of preparing a coating solution for a glucose sensing membrane, the method comprising preserving a first solution containing an oxidizing enzyme or a reducing enzyme of glucose and a reagent for generating a substance for coloring a coloring agent dissolved in a buffer solution, preserving a second solution containing a mixed solution of an alcohol solution of the coloring agent and a solution of a membrane-forming polymer compound, and mixing the first solution and the second solution.

摘要翻译： 一种制备葡萄糖感测膜的涂布溶液的方法，所述方法包括保存含有葡萄糖的氧化酶或还原酶的第一溶液和用于产生溶解在缓冲溶液中的着色剂着色物质的试剂，保存 第二溶液，其含有着色剂的醇溶液和成膜高分子化合物的溶液的混合溶液，并混合第一溶液和第二溶液。

公开(公告)号：US07410614B2

公开(公告)日：2008-08-12

申请号：US10899264

申请日：2004-07-26

申请人： Kenichi Uchiyama ,  Ikuo Uematsu ,  Kayoko Oomiya ,  Ichiro Tono ,  Hideo Eto ,  Isao Kishimoto ,  Naotada Okada ,  Masami Hirata

发明人： Kenichi Uchiyama ,  Ikuo Uematsu ,  Kayoko Oomiya ,  Ichiro Tono ,  Hideo Eto ,  Isao Kishimoto ,  Naotada Okada ,  Masami Hirata

IPC分类号： G01N21/00 ,  G02B6/10

CPC分类号： A61B5/14514 ,  A61B5/14532 ,  A61B5/1455

摘要： An optical waveguide type iontophoresis sensor chip has a substrate having a first hinged plate and a second hinged plate, an optical waveguide plate connected to the first hinged plate and positioned over a first opening delineated in the first hinged plate, and a gel layer fixed on the second hinged plate and covering a second opening delineated in the second hinged plate and configured to contact with the optical waveguide plate through the first opening in case that the substrate is folded.

摘要翻译： 光波导型离子电渗传感器芯片具有：具有第一铰接板和第二铰接板的基板;连接到第一铰接板的光波导板，定位在第一铰接板中描绘的第一开口上;以及固定在 所述第二铰接板并且覆盖在所述第二铰接板中描绘的第二开口，并且构造成在所述基板被折叠的情况下通过所述第一开口与所述光波导板接触。