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    Pharmaceutically tractable secondary drug targets, methods of identification and their use in the creation of small molecule therapeutics
    1.
    发明申请
    Pharmaceutically tractable secondary drug targets, methods of identification and their use in the creation of small molecule therapeutics 审中-公开
    可药用二级药物靶标，鉴定方法及其在创建小分子治疗剂中的应用

    公开(公告)号：US20050277149A1

    公开(公告)日：2005-12-15

    申请号：US11150435

    申请日：2005-06-13

    申请人： Stephen Friend ,  Leland Hartwell

    发明人： Stephen Friend ,  Leland Hartwell

    IPC分类号： C12N15/85 ,  C12Q1/68 ,  G01N33/574

    CPC分类号： G01N33/574 ,  G01N2500/00

    摘要： The present invention relates to a method of identifying one or more secondary drug targets and their use in the identification of drug or drug candidates, particularly for the treatment of cancer. The yeast-based synthetic lethal screens were used to functionally identify and validate new gene targets to kill tumor cells with defects in cell cycle checkpoints and damage response pathways. These newly identified gene targets can be used to develop new cancer chemotherapeutics.

    摘要翻译： 本发明涉及一种鉴定一种或多种次级药物靶标的方法及其在鉴定药物或药物候选物，特别是用于治疗癌症中的用途。 使用基于酵母的合成致死筛选来功能性鉴定和验证新的基因靶标以杀死具有细胞周期检查点和损伤反应途径缺陷的肿瘤细胞。 这些新确定的基因靶标可用于开发新的癌症化疗药物。

    Detection of proteins whose absence is associated with a neoplasm
    3.
    发明授权
    Detection of proteins whose absence is associated with a neoplasm 失效
    检测不存在与肿瘤相关的蛋白质

    公开(公告)号：US07223842B1

    公开(公告)日：2007-05-29

    申请号：US08255572

    申请日：1994-06-08

    申请人： Thaddeus P. Dryja ,  Stephen Friend

    发明人： Thaddeus P. Dryja ,  Stephen Friend

    IPC分类号： A61K39/29

    CPC分类号： C07K16/30 ,  G01N33/57484 ,  Y10S436/813

    摘要： This invention relates to nucleic acid, or fragments thereof, encoding the retinoblastoma polypeptide, the retinoblastoma polypeptide itself, methods of detecting a defective retinoblastoma gene in human patients, and methods of treating these patients.

    摘要翻译： 本发明涉及编码视网膜母细胞瘤多肽的核酸或其片段，视网膜母细胞瘤多肽本身，检测人类患者视网膜母细胞瘤缺陷基因的方法，以及治疗这些患者的方法。

    Iterative probe design and detailed expression profiling with flexible in-situ synthesis arrays
    4.
    发明申请
    Iterative probe design and detailed expression profiling with flexible in-situ synthesis arrays 审中-公开
    迭代探针设计和具有灵活原位合成阵列的详细表达谱

    公开(公告)号：US20060141501A1

    公开(公告)日：2006-06-29

    申请号：US11282025

    申请日：2005-11-16

    申请人： Stephen Friend ,  Roland Stoughton ,  Peter Linsley ,  Julia Burchard

    发明人： Stephen Friend ,  Roland Stoughton ,  Peter Linsley ,  Julia Burchard

    IPC分类号： C12Q1/68

    CPC分类号： G16B25/00

    摘要： Methods and compositions are provided that are useful for detecting and reporting a plurality of different target polynucleotide sequences in a sample, such as polynucleotides corresponding to a plurality of different genes expressed by a cell or cells. In particular, the invention provides methods for screening a plurality of candidate polynucleotide probes to evaluate both the sensitivity and the specificity with which each candidate probe hybridizes to a target polynucleoide sequence. Candidate polynucleotide probes can then be ranked according to both their sensitivity and specificity, and probes that have optimal sensitivity and specificity for a target polynucleotide sequence can be selected. In one embodiment, polynucleotide probes can be selected according to the methods described herein to prepare “screening chips” wherein a large number of target polynucleotide sequences are detected using a single microarray have a few (e.g., 1-5) probes for each target polynucleotide sequence. In a particularly preferred embodiment, the invention provides a screening chip that can detect genetic transcripts from the entire genome of an organism. In an alternative embodiment, polynucleotide probes can be selected according to the methods described herein to prepare “signature chips” to more accurately detect certain selected “signature genes” using several polynucleotide probes (e:g., 10-20) for each signature gene. The invention additionally provides microarrays containing polynucleotide probes for a large number of genes expressed by a cell or organism. Further, methods for detecting a plurality of polynucleotide molecules, including a large number of genes expressed by a cell or organism, are also provided.

    摘要翻译： 提供了可用于检测和报告样品中多个不同靶多核苷酸序列的方法和组合物，例如对应于由细胞或细胞表达的多种不同基因的多核苷酸。 特别地，本发明提供了筛选多个候选多核苷酸探针以评估每个候选探针与目标多核苷酸序列杂交的灵敏度和特异性的方法。 然后可以根据其灵敏度和特异性对候选多核苷酸探针进行排序，并且可以选择对靶多核苷酸序列具有最佳敏感性和特异性的探针。 在一个实施方案中，可以根据本文所述的方法选择多核苷酸探针以制备“筛选芯片”，其中使用单个微阵列检测大量目标多核苷酸序列，其中每个靶多核苷酸具有少量（例如1-5）探针 序列。 在一个特别优选的实施方案中，本发明提供了可以检测来自生物体的整个基因组的遗传转录物的筛选芯片。 在替代实施方案中，可以根据本文所述的方法选择多核苷酸探针，以制备“签名芯片”，以使用每个签名基因的多个多核苷酸探针（例如，10-20）更准确地检测某些所选择的“签名基因” 。 本发明另外提供含有由细胞或生物表达的大量基因的多核苷酸探针的微阵列。 此外，还提供了用于检测多个多核苷酸分子的方法，包括由细胞或生物体表达的大量基因。

    Retinoblastoma nucleic acids
    6.
    发明授权
    Retinoblastoma nucleic acids 失效
    视网膜母细胞瘤核酸

    公开(公告)号：US07384735B1

    公开(公告)日：2008-06-10

    申请号：US09387158

    申请日：1999-08-31

    申请人： Thaddeus P. Dryja ,  Stephen Friend ,  David W. Yandell

    发明人： Thaddeus P. Dryja ,  Stephen Friend ,  David W. Yandell

    IPC分类号： A61K39/29

    CPC分类号： C07K14/4736

    摘要： This invention relates to nucleic acid, or fragments thereof, encoding the retinoblastoma polypeptide, the retinoblastoma polypeptide itself, methods of detecting a defective retinoblastoma gene in human patients, and methods of treating these patients.

    摘要翻译： 本发明涉及编码视网膜母细胞瘤多肽的核酸或其片段，视网膜母细胞瘤多肽本身，检测人类患者视网膜母细胞瘤缺陷基因的方法，以及治疗这些患者的方法。

    Bottle retainer
    7.
    发明授权
    Bottle retainer 失效
    瓶盖

    公开(公告)号：US07017759B1

    公开(公告)日：2006-03-28

    申请号：US10674622

    申请日：2003-09-30

    申请人： Stephen Friend

    发明人： Stephen Friend

    IPC分类号： A47B73/00

    CPC分类号： A47B73/00

    摘要： A bottle retainer includes a base having an affixing portion and a retaining portion. The affixing portion is adapted to mount the bottle retainer to a surface and the retaining portion is adapted to slidably receive at least one bottle. The retaining portion may further include obstructions for engaging the bottle, whereby accidental disengagement is prevented. It is emphasized that this abstract is provided to comply with the rules requiring an abstract that will allow a searcher or other reader to quickly ascertain the subject matter of the technical disclosure. It is submitted with the understanding that it will not be used to interpret or limit the scope or meaning of the claims.

    摘要翻译： 瓶保持器包括具有固定部分和保持部分的基部。 固定部分适于将瓶保持器安装到表面上，并且保持部分适于可滑动地容纳至少一个瓶子。 保持部分还可以包括用于接合瓶子的障碍物，从而防止意外脱开。 要强调的是，该摘要被提供以符合要求抽象的规则，允许搜索者或其他读者快速确定技术公开内容的主题。 提交它的理解是，它不会用于解释或限制权利要求的范围或含义。

    Synthetic lethal screen using RNA interference
    8.
    发明申请
    Synthetic lethal screen using RNA interference 审中-公开
    合成致死屏幕采用RNA干扰

    公开(公告)号：US20050181385A1

    公开(公告)日：2005-08-18

    申请号：US10947637

    申请日：2004-09-22

    申请人： Peter Linsley ,  Mao Mao ,  Annette Kim ,  Stephen Friend ,  Steven Bartz ,  Michele Cleary

    发明人： Peter Linsley ,  Mao Mao ,  Annette Kim ,  Stephen Friend ,  Steven Bartz ,  Michele Cleary

    IPC分类号： C07H21/02 ,  C12N15/11 ,  C12N15/85 ,  C12Q1/68

    CPC分类号： C12N15/111 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53 ,  C12N2320/12

    摘要： The invention provides a method for identifying one or more genes in a cell of a cell type which interact with, e.g., modulate the effect of, an agent, e.g., a drug. For example, an identified gene may confer resistance or sensitivity to a drug, i.e., reduces or enhances the effect of the drug. The invention also provides STK6 and TPX2 as a gene that exhibits synthetic lethal interactions with KSP encoding a kinesin-like motor protein, and methods and compositions for treatment of diseases, e.g., cancers, by modulating the expression of STK6 or TPX2 gene and/or the activity of STK6 or TPX2 gene product. The invention also provides genes involved in cellular response to DNA damage, and their therapeutic uses.

    摘要翻译： 本发明提供了一种用于鉴定与例如药物的作用相互作用的细胞类型的细胞中的一种或多种基因的方法。 例如，鉴定的基因可赋予药物抗药性或敏感性，即降低或增强药物的作用。 本发明还提供STK6和TPX2作为与编码驱动蛋白样运动蛋白的KSP呈现合成的致死相互作用的基因，以及通过调节STK6或TPX2基因的表达和/或治疗疾病例如癌症的方法和组合物 STK6或TPX2基因产物的活性。 本发明还提供涉及对DNA损伤的细胞反应及其治疗用途的基因。