Patent search ap:("10X GENOMICS Page INC.") AND inv:"Andrew D. Price"

1.

发明授权
Methods and systems for characterizing nucleic acid molecules 有权

公开(公告)号：US11932899B2

公开(公告)日：2024-03-19

申请号：US16434068

申请日：2019-06-06

Applicant: 10X Genomics, Inc.

Inventor： Kamila Belhocine , Michael Schnall-Levin , Andrew D. Price

IPC: C12Q1/68

CPC classification number: C12Q1/68 , C12Q2537/164 , C12Q2563/149 , C12Q2563/185

Abstract: Provided herein are methods and systems for characterizing a nucleic acid molecule from a single cell. A method for characterizing a nucleic acid molecule from a single cell may comprise providing a partition (e.g., droplets or wells) comprising a single cell and a single bead. The single bead may comprise a nucleic acid barcode molecule. A nucleic acid molecule from the single cell and the nucleic acid barcode molecule may be used to generate a barcoded nucleic acid molecule for sequencing, which may be used to determine an epigenetic state or characteristic of the nucleic acid molecule as being associated with the single cell.

2.

发明公开
METHODS AND SYSTEMS FOR CELL AND BEAD PROCESSING 审中-公开

公开(公告)号：US20240003785A1

公开(公告)日：2024-01-04

申请号：US18205397

申请日：2023-06-02

Applicant: 10X GENOMICS, INC.

Inventor： Christopher Hindson , Andrew D. Price , Michael Schnall-Levin

IPC: G01N1/28 , G01N1/40 , C12N5/00

CPC classification number: G01N1/28 , G01N1/4077 , C12N5/0006 , C12N2537/10

Abstract: The present disclosure provides methods and systems for cell and bead processing or analysis. A method for processing a cell or bead may comprise subjecting a bead to conditions sufficient to change a first characteristic or set of characteristics (e.g., cell or bead size). Such a method may further comprise subjecting the cell or bead to conditions sufficient to change a second characteristic or set of characteristics. In some cases, crosslinks may be formed within the cell or bead.

3.

发明公开
SYSTEMS AND METHODS FOR INFERRING CELL STATUS 审中-公开

公开(公告)号：US20230377688A1

公开(公告)日：2023-11-23

申请号：US18304718

申请日：2023-04-21

Applicant: 10X Genomics, Inc.

Inventor： Michael Schnall-Levin , Rajiv Bharadwaj , Zahra Kamila Belhocine , Andrew D. Price , Yifeng Yin , Vijay Kumar Sreenivasa Gopalan , Zeljko Jovan Dzakula

IPC: G16B30/00 , G16H10/40 , G16H20/00

CPC classification number: G16B30/00 , G16H10/40 , G16H20/00

Abstract: Systems and methods for inferring a status of a cell population are provided. Described techniques allow deconvolving a first clonal population comprising a first plurality of cells of a species, wherein nucleic acid sequence reads from each cell in the first plurality of cells are obtained. The nucleic acid sequence reads are mapped into bins representing portions of a reference genome, and a pattern of sequence read counts for each cell across the multiple bins is used to assign a cell to a group, thereby inferring a mitotic status of the cell. The assignment of nucleic acid sequence reads into bins is also be used for segregating cells into classes based on a status of a certain biological marker in each cell. Comparison of sequence read counts for a subset of bins across the cell classes allows evaluating effect of a compound on a cell status.

4.

发明公开
METHODS AND SYSTEMS FOR PROCESSING POLYNUCLEOTIDES 审中-公开

公开(公告)号：US20230203577A1

公开(公告)日：2023-06-29

申请号：US17822117

申请日：2022-08-24

Applicant: 10x Genomics, Inc.

Inventor： Phillip Belgrader , Zachary Bent , Rajiv Bharadwaj , Vijay Kumar Sreenivasa Gopalan , Josephine Harada , Christopher Hindson , Mohammad Rahimi Lenji , Michael Ybarra Lucero , Geoffrey McDermott , Elliott Meer , Tarjei Sigurd Mikkelsen , Christopher Joachim O'Keeffe , Katherine Pfeiffer , Andrew D. Price , Paul Ryvkin , Serge Saxonov , John R. Stuelpnagel , Jessica Michele Terry , Tobias Daniel Wheeler , Indira Wu , Solongo Batjargal Ziraldo , Stephane Claude Boutet , Sarah Taylor , Niranjan Srinivas

IPC: C12Q1/6874 , C12Q1/683 , C12Q1/6806

CPC classification number: C12Q1/6874 , C12Q1/683 , C12Q1/6806 , C12Q2563/179 , C12Q2537/143 , C12Q2525/191

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

5.

发明授权
Compositions, methods, and systems for bead formation using improved polymers 有权

公开(公告)号：US11441172B2

公开(公告)日：2022-09-13

申请号：US17009352

申请日：2020-09-01

Applicant: 10X Genomics, Inc.

Inventor： Joshua Delaney , Shalini Gohil , Christopher Hindson , Adam Lowe , Andrew D. Price , Joseph Francis Shuga

IPC: C12Q1/6806 , B01J13/18 , B01J13/00 , C08J3/075 , C12Q1/6869

Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.

6.

发明申请
METHODS AND SYSTEMS FOR PROCESSING POLYNUCLEOTIDES 有权

公开(公告)号：US20210130892A1

公开(公告)日：2021-05-06

申请号：US17084481

申请日：2020-10-29

Applicant: 10X Genomics, Inc.

Inventor： Phillip Belgrader , Zachary Bent , Rajiv Bharadwaj , Vijay Kumar Sreenivasa Gopalan , Josephine Harada , Christopher Hindson , Mohammad Rahimi Lenji , Michael Ybarra Lucero , Geoffrey McDermott , Elliott Meer , Tarjei Sigurd Mikkelsen , Christopher Joachim O'Keeffe , Katherine Pfeiffer , Andrew D. Price , Paul Ryvkin , Serge Saxonov , John R. Stuelpnagel , Jessica Michele Terry , Tobias Daniel Wheeler , Indira Wu , Solongo Batjargal Ziraldo , Stephane Claude Boutet , Sarah Taylor , Niranjan Srinivas

IPC: C12Q1/6874 , C12Q1/683 , C12Q1/6806

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

7.

发明授权
Methods and systems for characterizing multiple analytes from individual cells or cell populations 有权

公开(公告)号：US10928386B2

公开(公告)日：2021-02-23

申请号：US16375119

申请日：2019-04-04

Applicant: 10X Genomics, Inc.

Inventor： Zahra Kamila Belhocine , Andrew D. Price , Michael Schnall-Levin

IPC: C40B30/04 , G01N33/532 , C07K14/74 , C12N15/85 , G01N33/543 , G01N33/548 , C12N15/10 , C12Q1/6804 , C12Q1/6806 , G01N33/50 , G01N33/53 , G01N33/58 , C12Q1/6818 , C12Q1/6827 , C12Q1/6881 , C12N15/11 , G01N33/569 , C40B70/00

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, epigenetic information (e.g., accessible chromatin, DNA methylation), and RNA molecules (e.g., mRNA or CRISPR guide RNAs). In some cases, the disclosed methods comprise analysis of analytes from a cell using a cell bead.

8.

发明授权
Compositions, methods, and systems for bead formation using improved polymers 有权

公开(公告)号：US10774369B2

公开(公告)日：2020-09-15

申请号：US16374112

申请日：2019-04-03

Applicant: 10X Genomics, Inc.

Inventor： Joshua Delaney , Shalini Gohil , Christopher Hindson , Adam Lowe , Andrew D. Price , Joseph Francis Shuga

IPC: C08J3/075 , C12Q1/6806 , B01J13/18 , B01J13/00 , C12Q1/6869

Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.