公开(公告)号：US20140234916A1

公开(公告)日：2014-08-21

申请号：US14230766

申请日：2014-03-31

Applicant: Ajinomoto Co., Inc.

Inventor： Yasuaki Takakura ,  Hiroomi Ogino ,  Masakazu Sugiyama ,  Kenichi Mori ,  Eri Tabuchi ,  Koki Ishikawa ,  Uno Tagami ,  Hidemi Fujii

IPC: C12P13/04

CPC classification number: C12P13/04 ,  C12N9/1096 ,  C12P17/10

Abstract: The present invention provides a methodology for improving a yield of 2R,4R-Monatin. Specifically, the present invention provides a method for producing 2S,4R-Monatin or a salt thereof, comprising contacting 4R-IHOG with an L-amino acid aminotransferase in the presence of an L-amino acid to form the 2S,4R-Monatin; a method for producing 2R,4R-Monatin or a salt thereof, comprising isomerizing the 2S,4R-Monatin to form the 2R,4R-Monatin; and the like. These production methods may further comprise condensing indole-3-pyruvate and pyruvate to form the 4R-IHOG, and deaminating a tryptophan to form the indole-3-pyruvate.

Abstract translation: 本发明提供了提高2R，4R-莫纳甜产率的方法。 具体地说，本发明提供了制备2S，4R-莫纳甜或其盐的方法，包括在L-氨基酸的存在下将4R-1HOG与L-氨基酸氨基转移酶接触以形成2S，4R-莫纳甜; 一种制备2R，4R-莫纳甜或其盐的方法，包括使2S，4R-莫纳甜异构化形成2R，4R-莫纳甜; 等等。 这些制备方法还可以包括将吲哚-3-丙酮酸和丙酮酸缩合形成4R-1HOG，并使色氨酸脱氨基形成吲哚-3-丙酮酸盐。

公开(公告)号：US10113161B2

公开(公告)日：2018-10-30

申请号：US15223662

申请日：2016-07-29

Applicant: AJINOMOTO CO., INC.

Inventor： Ayako Sasahara ,  Eri Tabuchi ,  Hideyuki Suzuki ,  Uno Tagami ,  Tatsuki Kashiwagi ,  Takayuki Ito ,  Hiroyuki Nozaki ,  Shunichi Suzuki

IPC: C12P21/02 ,  C12N9/00 ,  C07K5/02

Abstract: A mutant glutamate-cysteine ligase (GSHA) suitable for generating γ-Glu-Val, and a method for producing γ-Glu-Val-Gly using the same are provided. γ-Glu-Val is produced by using a mutant GSHA having a specific mutation with Glu and Val as raw materials, and γ-Glu-Val-Gly is further produced by using γ-Glu-Val and Gly as raw materials. γ-Glu-Val-Gly is produced by using a mutant GSHA having a specific mutation with Glu, Val, and Gly as raw materials.

公开(公告)号：US20160326510A1

公开(公告)日：2016-11-10

申请号：US15223662

申请日：2016-07-29

Applicant: AJINOMOTO CO., INC.

Inventor： Ayako SASAHARA ,  Eri Tabuchi ,  Hideyuki Suzuki ,  Uno Tagami ,  Tatsuki Kashiwagi ,  Takayuki Ito ,  Hiroyuki Nozaki ,  Shunichi Suzuki

IPC: C12N9/00 ,  C07K5/02 ,  C12P21/02

CPC classification number: C12N9/93 ,  C07K5/0215 ,  C12P21/02 ,  C12Y603/02002

Abstract: A mutant glutamate-cysteine ligase (GSHA) suitable for generating γ-Glu-Val, and a method for producing γ-Glu-Val-Gly using the same are provided. γ-Glu-Val is produced by using a mutant GSHA having a specific mutation with Glu and Val as raw materials, and γ-Glu-Val-Gly is further produced by using γ-Glu-Val and Gly as raw materials. γ-Glu-Val-Gly is produced by using a mutant GSHA having a specific mutation with Glu, Val, and Gly as raw materials.

Abstract translation: 提供适用于产生γ-Glu-Val的突变型谷氨酸 - 半胱氨酸连接酶（GSHA），以及使用其制备γ-Glu-Val-Gly的方法。 通过使用与Glu和Val作为原料具有特异性突变的突变体GSHA，通过使用γ-Glu-Val和Gly作为原料进一步制备γ-Glu-Val-Gly来产生γ-Glu-Val。 通过使用与Glu，Val和Gly作为原料具有特异性突变的突变体GSHA来产生γ-Glu-Val-Gly。