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公开(公告)号：US11788109B2
公开(公告)日：2023-10-17
申请号：US15911731
申请日：2018-03-05
Applicant: AJINOMOTO CO., INC.
Inventor： Ayako Sasahara , Takayuki Ito , Hiroyuki Nozaki
CPC classification number: C12P21/02 , C07K5/0806 , C07K5/0819 , C12N1/205 , C12N9/93 , C12N15/09 , C12R2001/19 , C12Y603/02002 , C12Y603/02003
Abstract: A microorganism useful as an expression host for γ-Glu-Val synthetase and a method for producing γ-Glu-Val-Gly using γ-Glu-Val synthetase expressed in the microorganism are provided. By using γ-Glu-Val synthetase expressed in a bacterium, such as Escherichia bacteria, modified so that the activity of a protein encoded by a ybdK gene (YBDIQ is reduced as an expression host, γ-Glu-Val-Gly is produced (Yom Glu, Val, and Gly as raw materials.
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公开(公告)号：US10537128B2
公开(公告)日：2020-01-21
申请号：US15352928
申请日：2016-11-16
Applicant: AJINOMOTO CO., INC.
Inventor： Hiroaki Nishiuchi , Misato Morita , Wataru Hoshino , Junko Yamazaki , Takayuki Ito , Kazuo Yamagishi
Abstract: A yeast extract containing 0.2% or more of γ-Glu-Abu based on dry weight of the yeast extract is produced by culturing a yeast, such as Saccharomyces cervisiae or Candida utilis, in a medium containing a compound selected from Abu (L-2-aminobutyric acid) and γ-Glu-Abu (L-γ-glutamyl-L-2-aminobutyric acid), and preparing a yeast extract from the obtained cells.
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3.发明申请YEAST, YEAST EXTRACT CONTAINING GAMMA-GLU-ABU, AND A METHOD FOR PRODUCING THE SAME 审中-公开含有GAMMA-GLU-ABU的YEAST，YEAST提取物及其生产方法公开(公告)号：US20130280381A1
公开(公告)日：2013-10-24
申请号：US13838065
申请日：2013-03-15
Applicant: Ajinomoto Co., Inc.
Inventor： Hiroaki Nishiuchi , Misato Morita , Wataru Hoshino , Junko Yamazaki , Takayuki Ito , Kazuo Yamagishi
CPC classification number: A23L31/15 , A23L5/00 , A23L27/22 , A23L27/24 , A23L31/00 , A23L33/14 , A23L33/145 , A23V2002/00 , C12N1/18 , C12N9/1096 , C12N9/93 , C12N15/81 , C12P13/04 , C12P13/14 , C12Y203/02002 , C12Y603/02002 , C12Y603/02003
Abstract: A yeast extract containing 0.2% or more of γ-Glu-Abu based on dry weight of the yeast extract is produced by culturing a yeast, such as Saccharomyces cervisiae or Candida utilis, in a medium containing a compound selected from Abu (L-2-aminobutyric acid) and γ-Glu-Abu (L-γ-glutamyl-L-2-aminobutyric acid), and preparing a yeast extract from the obtained cells.
Abstract translation: 通过在含有选自Abu（L-2）的化合物的培养基中培养酵母，例如酿酒酵母或产朊假丝酵母，通过酵母提取物的干重来含有0.2％以上的γ-Glu-Abu的酵母提取物 - 氨基丁酸）和γ-Glu-Abu（L-γ-谷氨酰基-L-2-氨基丁酸），并从所获得的细胞制备酵母提取物。
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Patent Agency Ranking
4.发明授权DNA encoding dipeptide-synthesizing enzyme (variants), bacterium belonging to the genus Escherichia, and methods for producing dipeptides using thereof 有权编码二肽合成酶（变体）的DNA，属于埃希氏菌属的细菌，以及使用其生产二肽的方法公开(公告)号：US09428783B2
公开(公告)日：2016-08-30
申请号：US14591387
申请日：2015-01-07
Applicant: Ajinomoto Co., Inc.
Inventor： Sergey Vasilievich Smirnov , Pavel Mikhailovich Sokolov , Takayuki Ito
CPC classification number: C12P21/00 , C07K5/06104 , C07K5/06113 , C12N9/93 , C12Y603/02028
Abstract: The present invention describes novel bacterial L-amino acids α-ligases, which catalyzing reaction of dipeptide formation having an acidic L-amino acid such as L-Asp or L-Glu at the N-terminus. The method for producing dipeptides using said L-amino acids α-ligases and a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to contain the DNA encoding said L-amino acids α-ligases, is described.
Abstract translation: 本发明描述了新型细菌L-氨基酸α-连接酶，其催化在N末端具有酸性L-氨基酸如L-Asp或L-Glu的二肽形成的反应。 使用所述L-氨基酸α-连接酶和已被修饰为含有编码所述L-氨基酸α-连接酶的DNA的肠杆菌科，特别是属于埃希氏菌属的细菌的细菌来生产二肽的方法是 描述。
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5.发明申请公开(公告)号：US20150197782A1
公开(公告)日：2015-07-16
申请号：US14591387
申请日：2015-01-07
Applicant: Ajinomoto Co., Inc.
Inventor： Sergey Vasilievich SMIRNOV , Pavel Mikhailovich Sokolov , Takayuki Ito
CPC classification number: C12P21/00 , C07K5/06104 , C07K5/06113 , C12N9/93 , C12Y603/02028
Abstract: The present invention describes novel bacterial L-amino acids α-ligases, which catalyzing reaction of dipeptide formation having an acidic L-amino acid such as L-Asp or L-Glu at the N-terminus. The method for producing dipeptides using said L-amino acids α-ligases and a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to contain the DNA encoding said L-amino acids α-ligases, is described.
Abstract translation: 本发明描述了新型细菌L-氨基酸α-连接酶，其催化在N末端具有酸性L-氨基酸如L-Asp或L-Glu的二肽形成的反应。 使用所述L-氨基酸α-连接酶和已被修饰为含有编码所述L-氨基酸α-连接酶的DNA的肠杆菌科，特别是属于埃希氏菌属的细菌的细菌来生产二肽的方法是 描述。
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公开(公告)号：US11142755B2
公开(公告)日：2021-10-12
申请号：US16287459
申请日：2019-02-27
Applicant: AJINOMOTO CO., INC.
Inventor： Ayako Sato , Eri Higashiura , Misato Okamoto , Takayuki Ito , Erika Watanabe , Uno Tagami , Yuki Oda , Tatsuki Kashiwagi , Masayuki Sugiki
Abstract: A mutant glutathione synthetase (GSHB) suitable for generating γ-Glu-Val-Gly, and a method for producing γ-Glu-Val-Gly using the same are provided. γ-Glu-Val-Gly is produced by using a mutant GSHB having a mutation at such a position as V7, N13, I14, N15, K17, F22, F95, M165, N199, Y200, P202, I274, T285, and P287.
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7.发明授权公开(公告)号：US10113161B2
公开(公告)日：2018-10-30
申请号：US15223662
申请日：2016-07-29
Applicant: AJINOMOTO CO., INC.
Inventor： Ayako Sasahara , Eri Tabuchi , Hideyuki Suzuki , Uno Tagami , Tatsuki Kashiwagi , Takayuki Ito , Hiroyuki Nozaki , Shunichi Suzuki
Abstract: A mutant glutamate-cysteine ligase (GSHA) suitable for generating γ-Glu-Val, and a method for producing γ-Glu-Val-Gly using the same are provided. γ-Glu-Val is produced by using a mutant GSHA having a specific mutation with Glu and Val as raw materials, and γ-Glu-Val-Gly is further produced by using γ-Glu-Val and Gly as raw materials. γ-Glu-Val-Gly is produced by using a mutant GSHA having a specific mutation with Glu, Val, and Gly as raw materials.
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8.发明申请MUTANT GLUTAMATE-CYSTEINE LIGASE AND METHOD FOR MANUFACTURING GAMMA-GLUTAMYL-VALYL-GLYCINE 审中-公开突变谷氨酸 - 酪氨酸激酶和制备GAMMA-谷氨酰 - 谷氨酸的方法公开(公告)号：US20160326510A1
公开(公告)日：2016-11-10
申请号：US15223662
申请日：2016-07-29
Applicant: AJINOMOTO CO., INC.
Inventor： Ayako SASAHARA , Eri Tabuchi , Hideyuki Suzuki , Uno Tagami , Tatsuki Kashiwagi , Takayuki Ito , Hiroyuki Nozaki , Shunichi Suzuki
CPC classification number: C12N9/93 , C07K5/0215 , C12P21/02 , C12Y603/02002
Abstract: A mutant glutamate-cysteine ligase (GSHA) suitable for generating γ-Glu-Val, and a method for producing γ-Glu-Val-Gly using the same are provided. γ-Glu-Val is produced by using a mutant GSHA having a specific mutation with Glu and Val as raw materials, and γ-Glu-Val-Gly is further produced by using γ-Glu-Val and Gly as raw materials. γ-Glu-Val-Gly is produced by using a mutant GSHA having a specific mutation with Glu, Val, and Gly as raw materials.
Abstract translation: 提供适用于产生γ-Glu-Val的突变型谷氨酸 - 半胱氨酸连接酶（GSHA），以及使用其制备γ-Glu-Val-Gly的方法。 通过使用与Glu和Val作为原料具有特异性突变的突变体GSHA，通过使用γ-Glu-Val和Gly作为原料进一步制备γ-Glu-Val-Gly来产生γ-Glu-Val。 通过使用与Glu，Val和Gly作为原料具有特异性突变的突变体GSHA来产生γ-Glu-Val-Gly。
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