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公开(公告)号：US09580696B2
公开(公告)日：2017-02-28
申请号：US14243458
申请日：2014-04-02
Applicant: Ajinomoto Co., Inc.
Inventor： Hiroyuki Nozaki , Isao Abe , Jun Takakura , Rie Takeshita , Hideyuki Suzuki , Shunichi Suzuki
CPC classification number: C12P21/02 , C07K5/0215 , C07K5/0819 , C12N9/104 , C12N15/70 , C12Y203/02002 , Y02P20/52
Abstract: A method for producing γ-Glu-Val-Gly comprising the step of reacting Val-Gly with a γ-glutamyl group donor in the presence of a γ-glutamyltransferase, a microorganism containing the enzyme, or a processed product thereof to generate γ-Glu-Val-Gly, wherein the γ-glutamyltransferase consists of a large subunit and a small subunit, and the small subunit has a specific mutation.
Abstract translation: 一种γ-Glu-Val-Gly的制造方法，其特征在于，在γ-谷氨酰基转移酶，含有该微生物的微生物或其加工物的存在下，使Val-Gly与γ-谷氨酰供体反应， Glu-Val-Gly，其中γ-谷氨酰转移酶由大亚基和小亚基组成，小亚基具有特异性突变。
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Patent Agency Ranking
公开(公告)号：US09677106B2
公开(公告)日：2017-06-13
申请号：US15235311
申请日：2016-08-12
Applicant: Ajinomoto Co., Inc.
Inventor： Hiroyuki Nozaki , Isao Abe , Jun Takakura , Rie Takeshita , Hideyuki Suzuki , Shunichi Suzuki
CPC classification number: C12P21/02 , C07K5/0215 , C07K5/0819 , C12N9/104 , C12N15/70 , C12Y203/02002 , Y02P20/52
Abstract: A method for producing γ-Glu-Val-Gly comprising the step of reacting Val-Gly with a γ-glutamyl group donor in the presence of a γ-glutamyltransferase, a microorganism containing the enzyme, or a processed product thereof to generate γ-Glu-Val-Gly, wherein the γ-glutamyltransferase consists of a large subunit and a small subunit, and the small subunit has a specific mutation.
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公开(公告)号：US20160355861A1
公开(公告)日：2016-12-08
申请号：US15235311
申请日：2016-08-12
Applicant: Ajinomoto Co., Inc.
Inventor： Hiroyuki NOZAKI , Isao Abe , Jun Takakura , Rie Takeshita , Hideyuki Suzuki , Shunichi Suzuki
CPC classification number: C12P21/02 , C07K5/0215 , C07K5/0819 , C12N9/104 , C12N15/70 , C12Y203/02002 , Y02P20/52
Abstract: A method for producing γ-Glu-Val-Gly comprising the step of reacting Val-Gly with a γ-glutamyl group donor in the presence of a γ-glutamyltransferase, a microorganism containing the enzyme, or a processed product thereof to generate γ-Glu-Val-Gly, wherein the γ-glutamyltransferase consists of a large subunit and a small subunit, and the small subunit has a specific mutation.
Abstract translation: 一种γ-Glu-Val-Gly的制造方法，其特征在于，在γ-谷氨酰基转移酶，含有该微生物的微生物或其加工物的存在下，使Val-Gly与γ-谷氨酰基供体反应， Glu-Val-Gly，其中γ-谷氨酰转移酶由大亚基和小亚基组成，小亚基具有特异性突变。
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4.发明申请MUTANT GAMMA-GLUTAMYLTRANSFERASE, AND A METHOD FOR PRODUCING GAMMA-GLUTAMYLVALYLGLYCINE OR A SALT THEREOF 有权突变GAMMA-GLUTAMYLTRANSFERASE，以及一种生产GAMMA-谷氨酰乙酰胆碱或其盐的方法公开(公告)号：US20140212920A1
公开(公告)日：2014-07-31
申请号：US14243458
申请日：2014-04-02
Applicant: Ajinomoto Co., Inc.
Inventor： Hiroyuki NOZAKI , Isao Abe , Jun Takakura , Rie Takeshita , Hideyuki Suzuki , Shunichi Suzuki
CPC classification number: C12P21/02 , C07K5/0215 , C07K5/0819 , C12N9/104 , C12N15/70 , C12Y203/02002 , Y02P20/52
Abstract: A method for producing γ-Glu-Val-Gly comprising the step of reacting Val-Gly with a γ-glutamyl group donor in the presence of a γ-glutamyltransferase, a microorganism containing the enzyme, or a processed product thereof to generate γ-Glu-Val-Gly, wherein the γ-glutamyltransferase consists of a large subunit and a small subunit, and the small subunit has a specific mutation.
Abstract translation: 一种γ-Glu-Val-Gly的制造方法，其特征在于，在γ-谷氨酰基转移酶，含有该微生物的微生物或其加工物的存在下，使Val-Gly与γ-谷氨酰基供体反应， Glu-Val-Gly，其中γ-谷氨酰转移酶由大亚基和小亚基组成，小亚基具有特异性突变。
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5.发明授权公开(公告)号：US10113161B2
公开(公告)日：2018-10-30
申请号：US15223662
申请日：2016-07-29
Applicant: AJINOMOTO CO., INC.
Inventor： Ayako Sasahara , Eri Tabuchi , Hideyuki Suzuki , Uno Tagami , Tatsuki Kashiwagi , Takayuki Ito , Hiroyuki Nozaki , Shunichi Suzuki
Abstract: A mutant glutamate-cysteine ligase (GSHA) suitable for generating γ-Glu-Val, and a method for producing γ-Glu-Val-Gly using the same are provided. γ-Glu-Val is produced by using a mutant GSHA having a specific mutation with Glu and Val as raw materials, and γ-Glu-Val-Gly is further produced by using γ-Glu-Val and Gly as raw materials. γ-Glu-Val-Gly is produced by using a mutant GSHA having a specific mutation with Glu, Val, and Gly as raw materials.
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6.发明申请MUTANT GLUTAMATE-CYSTEINE LIGASE AND METHOD FOR MANUFACTURING GAMMA-GLUTAMYL-VALYL-GLYCINE 审中-公开突变谷氨酸 - 酪氨酸激酶和制备GAMMA-谷氨酰 - 谷氨酸的方法公开(公告)号：US20160326510A1
公开(公告)日：2016-11-10
申请号：US15223662
申请日：2016-07-29
Applicant: AJINOMOTO CO., INC.
Inventor： Ayako SASAHARA , Eri Tabuchi , Hideyuki Suzuki , Uno Tagami , Tatsuki Kashiwagi , Takayuki Ito , Hiroyuki Nozaki , Shunichi Suzuki
CPC classification number: C12N9/93 , C07K5/0215 , C12P21/02 , C12Y603/02002
Abstract: A mutant glutamate-cysteine ligase (GSHA) suitable for generating γ-Glu-Val, and a method for producing γ-Glu-Val-Gly using the same are provided. γ-Glu-Val is produced by using a mutant GSHA having a specific mutation with Glu and Val as raw materials, and γ-Glu-Val-Gly is further produced by using γ-Glu-Val and Gly as raw materials. γ-Glu-Val-Gly is produced by using a mutant GSHA having a specific mutation with Glu, Val, and Gly as raw materials.
Abstract translation: 提供适用于产生γ-Glu-Val的突变型谷氨酸 - 半胱氨酸连接酶（GSHA），以及使用其制备γ-Glu-Val-Gly的方法。 通过使用与Glu和Val作为原料具有特异性突变的突变体GSHA，通过使用γ-Glu-Val和Gly作为原料进一步制备γ-Glu-Val-Gly来产生γ-Glu-Val。 通过使用与Glu，Val和Gly作为原料具有特异性突变的突变体GSHA来产生γ-Glu-Val-Gly。
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