公开(公告)号：US20140212920A1

公开(公告)日：2014-07-31

申请号：US14243458

申请日：2014-04-02

Applicant: Ajinomoto Co., Inc.

Inventor： Hiroyuki NOZAKI ,  Isao Abe ,  Jun Takakura ,  Rie Takeshita ,  Hideyuki Suzuki ,  Shunichi Suzuki

IPC: C12N9/10 ,  C07K5/093

CPC classification number: C12P21/02 ,  C07K5/0215 ,  C07K5/0819 ,  C12N9/104 ,  C12N15/70 ,  C12Y203/02002 ,  Y02P20/52

Abstract: A method for producing γ-Glu-Val-Gly comprising the step of reacting Val-Gly with a γ-glutamyl group donor in the presence of a γ-glutamyltransferase, a microorganism containing the enzyme, or a processed product thereof to generate γ-Glu-Val-Gly, wherein the γ-glutamyltransferase consists of a large subunit and a small subunit, and the small subunit has a specific mutation.

Abstract translation: 一种γ-Glu-Val-Gly的制造方法，其特征在于，在γ-谷氨酰基转移酶，含有该微生物的微生物或其加工物的存在下，使Val-Gly与γ-谷氨酰基供体反应， Glu-Val-Gly，其中γ-谷氨酰转移酶由大亚基和小亚基组成，小亚基具有特异性突变。

公开(公告)号：US09580696B2

公开(公告)日：2017-02-28

申请号：US14243458

申请日：2014-04-02

Applicant: Ajinomoto Co., Inc.

Inventor： Hiroyuki Nozaki ,  Isao Abe ,  Jun Takakura ,  Rie Takeshita ,  Hideyuki Suzuki ,  Shunichi Suzuki

IPC: C12P21/02 ,  C12N9/10 ,  C07K5/093 ,  C07K5/02

CPC classification number: C12P21/02 ,  C07K5/0215 ,  C07K5/0819 ,  C12N9/104 ,  C12N15/70 ,  C12Y203/02002 ,  Y02P20/52

Abstract: A method for producing γ-Glu-Val-Gly comprising the step of reacting Val-Gly with a γ-glutamyl group donor in the presence of a γ-glutamyltransferase, a microorganism containing the enzyme, or a processed product thereof to generate γ-Glu-Val-Gly, wherein the γ-glutamyltransferase consists of a large subunit and a small subunit, and the small subunit has a specific mutation.

Abstract translation: 一种γ-Glu-Val-Gly的制造方法，其特征在于，在γ-谷氨酰基转移酶，含有该微生物的微生物或其加工物的存在下，使Val-Gly与γ-谷氨酰供体反应， Glu-Val-Gly，其中γ-谷氨酰转移酶由大亚基和小亚基组成，小亚基具有特异性突变。

公开(公告)号：US10113161B2

公开(公告)日：2018-10-30

申请号：US15223662

申请日：2016-07-29

Applicant: AJINOMOTO CO., INC.

Inventor： Ayako Sasahara ,  Eri Tabuchi ,  Hideyuki Suzuki ,  Uno Tagami ,  Tatsuki Kashiwagi ,  Takayuki Ito ,  Hiroyuki Nozaki ,  Shunichi Suzuki

IPC: C12P21/02 ,  C12N9/00 ,  C07K5/02

Abstract: A mutant glutamate-cysteine ligase (GSHA) suitable for generating γ-Glu-Val, and a method for producing γ-Glu-Val-Gly using the same are provided. γ-Glu-Val is produced by using a mutant GSHA having a specific mutation with Glu and Val as raw materials, and γ-Glu-Val-Gly is further produced by using γ-Glu-Val and Gly as raw materials. γ-Glu-Val-Gly is produced by using a mutant GSHA having a specific mutation with Glu, Val, and Gly as raw materials.

公开(公告)号：US20160326510A1

公开(公告)日：2016-11-10

申请号：US15223662

申请日：2016-07-29

Applicant: AJINOMOTO CO., INC.

Inventor： Ayako SASAHARA ,  Eri Tabuchi ,  Hideyuki Suzuki ,  Uno Tagami ,  Tatsuki Kashiwagi ,  Takayuki Ito ,  Hiroyuki Nozaki ,  Shunichi Suzuki

IPC: C12N9/00 ,  C07K5/02 ,  C12P21/02

CPC classification number: C12N9/93 ,  C07K5/0215 ,  C12P21/02 ,  C12Y603/02002

Abstract: A mutant glutamate-cysteine ligase (GSHA) suitable for generating γ-Glu-Val, and a method for producing γ-Glu-Val-Gly using the same are provided. γ-Glu-Val is produced by using a mutant GSHA having a specific mutation with Glu and Val as raw materials, and γ-Glu-Val-Gly is further produced by using γ-Glu-Val and Gly as raw materials. γ-Glu-Val-Gly is produced by using a mutant GSHA having a specific mutation with Glu, Val, and Gly as raw materials.

Abstract translation: 提供适用于产生γ-Glu-Val的突变型谷氨酸 - 半胱氨酸连接酶（GSHA），以及使用其制备γ-Glu-Val-Gly的方法。 通过使用与Glu和Val作为原料具有特异性突变的突变体GSHA，通过使用γ-Glu-Val和Gly作为原料进一步制备γ-Glu-Val-Gly来产生γ-Glu-Val。 通过使用与Glu，Val和Gly作为原料具有特异性突变的突变体GSHA来产生γ-Glu-Val-Gly。

公开(公告)号：US09677106B2

公开(公告)日：2017-06-13

申请号：US15235311

申请日：2016-08-12

Applicant: Ajinomoto Co., Inc.

Inventor： Hiroyuki Nozaki ,  Isao Abe ,  Jun Takakura ,  Rie Takeshita ,  Hideyuki Suzuki ,  Shunichi Suzuki

IPC: C12P21/02 ,  C07K5/093 ,  C12N9/10 ,  C12N15/70 ,  C07K5/02

CPC classification number: C12P21/02 ,  C07K5/0215 ,  C07K5/0819 ,  C12N9/104 ,  C12N15/70 ,  C12Y203/02002 ,  Y02P20/52

Abstract: A method for producing γ-Glu-Val-Gly comprising the step of reacting Val-Gly with a γ-glutamyl group donor in the presence of a γ-glutamyltransferase, a microorganism containing the enzyme, or a processed product thereof to generate γ-Glu-Val-Gly, wherein the γ-glutamyltransferase consists of a large subunit and a small subunit, and the small subunit has a specific mutation.

公开(公告)号：US20160355861A1

公开(公告)日：2016-12-08

申请号：US15235311

申请日：2016-08-12

Applicant: Ajinomoto Co., Inc.

Inventor： Hiroyuki NOZAKI ,  Isao Abe ,  Jun Takakura ,  Rie Takeshita ,  Hideyuki Suzuki ,  Shunichi Suzuki

IPC: C12P21/02 ,  C12N15/70 ,  C12N9/10

CPC classification number: C12P21/02 ,  C07K5/0215 ,  C07K5/0819 ,  C12N9/104 ,  C12N15/70 ,  C12Y203/02002 ,  Y02P20/52

Abstract: A method for producing γ-Glu-Val-Gly comprising the step of reacting Val-Gly with a γ-glutamyl group donor in the presence of a γ-glutamyltransferase, a microorganism containing the enzyme, or a processed product thereof to generate γ-Glu-Val-Gly, wherein the γ-glutamyltransferase consists of a large subunit and a small subunit, and the small subunit has a specific mutation.

Abstract translation: 一种γ-Glu-Val-Gly的制造方法，其特征在于，在γ-谷氨酰基转移酶，含有该微生物的微生物或其加工物的存在下，使Val-Gly与γ-谷氨酰基供体反应， Glu-Val-Gly，其中γ-谷氨酰转移酶由大亚基和小亚基组成，小亚基具有特异性突变。

公开(公告)号：US20130164792A1

公开(公告)日：2013-06-27

申请号：US13678909

申请日：2012-11-16

Applicant: Ajinomoto Co., Inc.

Inventor： Kenichi MORI ,  Masakazu Sugiyama ,  Mika Moriya ,  Shunichi Suzuki ,  Hidemi Fujii ,  Kyohei Fujiwara

IPC: C07D209/20 ,  C12P17/10

CPC classification number: C07D209/20 ,  C12P17/10 ,  C12Y206/01 ,  C12Y501/01

Abstract: The present invention provides a method of efficiently producing a crystal of a (2R,4R)Monatin multivalent metal salt that has a good sweetness property and is excellent in storage stability. Specifically, the present invention provides the method of producing the crystal of the (2R,4R)Monatin multivalent metal salt comprising allowing an aldehyde or one or two or more enzymes capable of forming (2R,4R)Monatin from (2S,4R)Monatin to be acted on an aqueous solution containing the (2S,4R)Monatin in the presence of a multivalent metal ion to obtain the crystal of the (2R,4R)Monatin multivalent metal salt. The aldehyde may preferably be an aromatic aldehyde. The one or two or more enzyme may preferably be a racemase or one or more aminotransferases. The multivalent metal may preferably be a bivalent alkaline earth metal.

Abstract translation: 本发明提供一种有效地制备具有良好的甜味性并且保存稳定性优异的（2R，4R）莫纳甜多金属盐晶体的方法。 具体地说，本发明提供了制备（2R，4R）莫纳甜多价金属盐晶体的方法，包括使（2，4R）莫纳甜能形成（2R，4R）莫纳甜的醛或一种或两种以上的酶 在含有（2S，4R）莫纳甜的水溶液中，在多价金属离子的存在下作用，得到（2R，4R）莫纳甜多晶金属盐的结晶。 醛可优选为芳族醛。 一种或两种或更多种酶可优选为消旋酶或一种或多种氨基转移酶。 多价金属可优选为二价碱土金属。