公开(公告)号：US20120145926A1

公开(公告)日：2012-06-14

申请号：US13402149

申请日：2012-02-22

Applicant: Eric J. Seibel ,  Alan C. Nelson ,  Mark E. Fauver ,  J. Richard Rahn

Inventor： Eric J. Seibel ,  Alan C. Nelson ,  Mark E. Fauver ,  J. Richard Rahn

IPC: G01N21/64

CPC classification number: G01N21/4795 ,  G01N15/1436 ,  G01N2015/1445 ,  G01N2021/1787 ,  G06T11/006

Abstract: A method for 3D imaging of cells in an optical tomography system includes moving a biological object relatively to a microscope objective to present varying angles of view. The biological object is illuminated with radiation having a spectral bandwidth limited to wavelengths between 150 nm and 390 nm. Radiation transmitted through the biological object and the microscope objective is sensed with a camera from a plurality of differing view angles. A plurality of pseudoprojections of the biological object from the sensed radiation is formed and the plurality of pseudoprojections is reconstructed to form a 3D image of the cell.

Abstract translation: 用于光学断层摄影系统中的细胞的3D成像的方法包括相对于显微镜物镜移动生物体以呈现不同的视角。 用具有限制在150nm和390nm之间的波长的光谱带宽的辐射照射该生物物体。 利用摄像机从多个不同的视角检测透过生物物体和显微镜物镜的辐射。 形成来自感测辐射的生物物体的多个伪投影，并重建多个伪投影以形成该细胞的3D图像。

公开(公告)号：US07811825B2

公开(公告)日：2010-10-12

申请号：US10964508

申请日：2004-10-13

Applicant: Mark E. Fauver ,  J. Richard Rahn ,  Eric J. Seibel ,  Alan C. Nelson

Inventor： Mark E. Fauver ,  J. Richard Rahn ,  Eric J. Seibel ,  Alan C. Nelson

IPC: G01N33/48

CPC classification number: G02B21/002 ,  G02B21/365

Abstract: A scanning method for scanning samples of biological cells using optical tomography includes preparing, acquiring, reconstructing and viewing three-dimensional images of cell samples. Concentration and enrichment of the cell sample follows. The cell sample is stained. Cells are isolated from the cell sample and purified. A cell/solvent mixture is injected into a gel by centrifugation. A cell/gel mixture is injected into a capillary tube until a cell appears centered in a field of view using a stopped-flow method. An optical imaging system, such as a fixed or variable motion optical tomography system acquires a projection image. The sample is rotated about a tube axis to generate additional projections. Once image acquisition is completed, the acquired image projections are corrected for errors. A computer or other equivalent processor is used to compute filtered backprojection information for 3D reconstruction.

Abstract translation: 使用光学断层摄影扫描生物细胞样本的扫描方法包括准备，获取，重建和观察细胞样品的三维图像。 细胞样品的浓缩和浓缩如下。 细胞样品染色。 细胞从细胞样品中分离并纯化。 通过离心将细胞/溶剂混合物注入凝胶。 将细胞/凝胶混合物注入毛细管中，直到细胞以视野停止流出中心为止。 诸如固定或可变运动光学断层摄影系统的光学成像系统获取投影图像。 样品围绕管轴旋转以产生额外的突起。 一旦图像获取完成，就获得的图像投影被校正以用于错误。 计算机或其他等效处理器用于计算用于3D重建的滤波反投影信息。

公开(公告)号：US07738945B2

公开(公告)日：2010-06-15

申请号：US10716744

申请日：2003-11-18

Applicant: Mark E. Fauver ,  J. Richard Rahn ,  Eric J. Seibel ,  Alan C. Nelson

Inventor： Mark E. Fauver ,  J. Richard Rahn ,  Eric J. Seibel ,  Alan C. Nelson

IPC: A61B5/05

CPC classification number: G01N15/1475 ,  G01N15/1468 ,  G01N15/147 ,  G01N21/05 ,  G01N21/4795 ,  G01N2015/1006 ,  G01N2015/1075 ,  G01N2015/1447 ,  G01N2021/0346 ,  G01N2021/1787 ,  G01N2201/0813 ,  G01N2223/612 ,  G06T11/006 ,  G06T2211/421

Abstract: A system for optical imaging of a thick specimen that permits rapid acquisition of data necessary for tomographic reconstruction of the three-dimensional (3D) image. One method involves the scanning of the focal plane of an imaging system and integrating the range of focal planes onto a detector. The focal plane of an optical imaging system is scanned along the axis perpendicular to said plane through the thickness of a specimen during a single detector exposure. Secondly, methods for reducing light scatter when using illumination point sources are presented. Both approaches yield shadowgrams. This process is repeated from multiple perspectives, either in series using a single illumination/detection subsystem, or in parallel using several illumination/detection subsystems. A set of pseudo-projections is generated, which are input to a three dimensional tomographic image reconstruction algorithm.

Abstract translation: 一种用于光学成像厚样本的系统，其允许快速获取三维（3D）图像的层析成像重建所需的数据。 一种方法涉及扫描成像系统的焦平面并将焦平面的范围集成到检测器上。 在单个检测器曝光期间，光学成像系统的焦平面沿着垂直于所述平面的轴线扫描样本的厚度。 其次，提出了使用照明点源时减少光散射的方法。 两种方法产生阴影图。 该过程从多个角度重复，使用单个照明/检测子系统串联使用，或者使用几个照明/检测子系统并行重复。 生成一组伪投影，其被输入到三维断层图像重建算法。

公开(公告)号：US06522775B2

公开(公告)日：2003-02-18

申请号：US09927151

申请日：2001-08-10

Applicant: Alan C. Nelson

Inventor： Alan C. Nelson

IPC: G06K900

CPC classification number: G06T11/006 ,  G01N15/147 ,  G01N21/05 ,  G01N23/046 ,  G01N2021/0346 ,  G01N2223/419

Abstract: A flow optical tomography system includes a flow cytometer, and at least one reconstruction cylinder positioned around a capillary tube. A photon source and a photon sensor work together with a pulse height analyzer to provide a first trigger point for the beginning of an object or cell, and a second trigger point for the end of the cell. The trigger signal is received by the reconstruction cylinder. The reconstruction cylinder includes optical point sources having a selectable emission wavelength, disposed in a geometric pattern around the cylinder perpendicular to and concentric with the capillary tube axis that facilitate the acquisition of transmitted, attenuated projection images of the flowing cells. The sensors also collect projections of fluorescence emitted from tagged molecular probes associated with nuclear and/or cytoplasmic structures or cell membranes. The projections are algorithmically processed to provide three dimensional information about the cells and their disease state.

公开(公告)号：US5799101A

公开(公告)日：1998-08-25

申请号：US897392

申请日：1997-07-21

Applicant: Shih-Jong J. Lee ,  Seho Oh ,  Stanley F. Patten ,  Alan C. Nelson ,  Larry A. Nelson

Inventor： Shih-Jong J. Lee ,  Seho Oh ,  Stanley F. Patten ,  Alan C. Nelson ,  Larry A. Nelson

IPC: G06K9/00 ,  G06K9/68 ,  G01N33/48

CPC classification number: G06K9/6282 ,  G06K9/00127 ,  Y10S128/922

Abstract: A computer aided biological specimen screener is responsive to a priori information about biological specimens. An automated slide classifier scans a slide specimen and generates a set of scores. The classification which results from this set of scores depends on a priori information concerning the patient, such as patient age and prior health history and diagnosis. Using the patient information and an appropriate score, the specimen is classified. A normal threshold used for classification is changed over a wide range depending on the specificity appropriate to a particular patient risk category. The sensitivity of the classification is automatically maximized for a predetermined normal specificity.

Abstract translation: 计算机辅助生物样本筛选器响应关于生物样本的先验信息。 自动滑块分类器扫描幻灯片样本并生成一组分数。 由该分数得出的分类取决于患者的先验信息，例如患者年龄和以前的健康史和诊断。 使用患者信息和适当的分数，将样本分类。 用于分类的正常阈值根据对特定患者风险类别的特异性而在宽范围内变化。 对于预定的正常特异性，分类的灵敏度自动最大化。

公开(公告)号：US07907765B2

公开(公告)日：2011-03-15

申请号：US11532648

申请日：2006-09-18

Applicant: Mark E. Fauver ,  Eric J. Seibel ,  Michael G. Meyer ,  Alan C. Nelson ,  J. Richard Rahn ,  Thomas Neumann ,  Roger H. Johnson

Inventor： Mark E. Fauver ,  Eric J. Seibel ,  Michael G. Meyer ,  Alan C. Nelson ,  J. Richard Rahn ,  Thomas Neumann ,  Roger H. Johnson

IPC: G06K9/00

CPC classification number: G01N21/4795 ,  G01N15/147 ,  G01N15/1475

Abstract: An optical tomography system for imaging an object of interest including a light source for illuminating the object of interest with a plurality of radiation beams. The object of interest is held within an object containing tube such that it is illuminated by the plurality of radiation beams to produce emerging radiation from the object containing tube, a detector array is located to receive the emerging radiation and produce imaging data used by a mechanism for tracking the object of interest.

Abstract translation: 一种光学断层摄影系统，用于对感兴趣的物体成像，包括用多个辐射束照射感兴趣物体的光源。 感兴趣的物体被保持在物体容纳管内，使得其被多个辐射束照射以产生来自物体容纳管的出射辐射，检测器阵列被定位成接收新出现的辐射并产生由机构使用的成像数据 用于跟踪感兴趣的对象。

公开(公告)号：US20100322494A1

公开(公告)日：2010-12-23

申请号：US11532648

申请日：2006-09-18

Applicant: Mark E. Fauver ,  Eric J. Seibel ,  Michael G. Meyer ,  Alan C. Nelson ,  J. Richard Rahn ,  Thomas Neumann ,  Roger H. Johnson

Inventor： Mark E. Fauver ,  Eric J. Seibel ,  Michael G. Meyer ,  Alan C. Nelson ,  J. Richard Rahn ,  Thomas Neumann ,  Roger H. Johnson

IPC: G06K9/00

CPC classification number: G01N21/4795 ,  G01N15/147 ,  G01N15/1475

Abstract: An optical tomography system for imaging an object of interest including a light source for illuminating the object of interest with a plurality of radiation beams. The object of interest is held within an object containing tube such that it is illuminated by the plurality of radiation beams to produce emerging radiation from the object containing tube, a detector array is located to receive the emerging radiation and produce imaging data used by a mechanism for tracking the object of interest.

Abstract translation: 一种光学断层摄影系统，用于对感兴趣的物体成像，包括用多个辐射束照射感兴趣物体的光源。 感兴趣的物体被保持在物体容纳管内，使得其被多个辐射束照射以产生来自物体容纳管的出射辐射，检测器阵列被定位成接收新出现的辐射并产生由机构使用的成像数据 用于跟踪感兴趣的对象。

公开(公告)号：US07542597B2

公开(公告)日：2009-06-02

申请号：US11558593

申请日：2006-11-10

Applicant: John Richard Rahn ,  Alan C. Nelson

Inventor： John Richard Rahn ,  Alan C. Nelson

IPC: G06K9/00 ,  G06K9/40 ,  G06K9/64 ,  G06K9/32

CPC classification number: G02B21/26 ,  G01N15/1468 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/05 ,  G01N21/4795 ,  G01N2015/1006 ,  G01N2015/1075 ,  G01N2021/0346 ,  G01N2021/1787 ,  G01N2201/0813 ,  G01N2223/612 ,  G02B21/365 ,  G06T11/005 ,  G06T11/006 ,  G06T2211/412 ,  G06T2211/421

Abstract: An apparatus and method for correction of relative object-detector motion between successive views during optical tomographic imaging in three dimensions. An object of interest is illuminated to produce an image. A lateral offset correction value is determined for the image. An axial offset correction value is determined for the image. The lateral offset correction value and the axial offset correction value are applied to the image to produce a corrected file image.

Abstract translation: 一种用于在三维尺度下在光学层析成像期间校正连续视图之间的相对物体检测器运动的装置和方法。 感兴趣的对象被照亮以产生图像。 确定图像的横向偏移校正值。 为图像确定轴向偏移校正值。 将横向偏移校正值和轴向偏移校正值应用于图像以产生校正的文件图像。

公开(公告)号：US07197355B2

公开(公告)日：2007-03-27

申请号：US10126026

申请日：2002-04-19

Applicant: Alan C. Nelson

Inventor： Alan C. Nelson

IPC: A61B5/05

CPC classification number: G01N15/1475 ,  G01N15/147 ,  G01N21/4795 ,  G01N2015/1006 ,  G01N2015/1075 ,  G01N2015/1445 ,  G01N2201/0813 ,  G01N2223/612 ,  G06T11/006 ,  G06T2211/421

Abstract: Motion of an object of interest, such as a cell, has a variable velocity that can be varied on a cell-by-cell basis. Cell velocity is controlled in one example by packing cells into a capillary tube, or any other linear substrate that provides optically equivalent 360 degree viewing access, so that the cells are stationary within the capillary tube, but the capillary tube is translated and rotated mechanically through a variable motion optical tomography reconstruction cylinder. The capillary tube motion may advantageously be controlled in a start-and-stop fashion and translated and rotated at any velocity for any motion interval, under the control of a computer program. As such, there are several configurations of the optical tomography system that take advantage of this controlled motion capability. Additionally, the use of polarization filters and phase plates to reduce light scatter and diffraction background noise is described.

Abstract translation: 感兴趣对象（诸如单元）的运动具有可以逐个细胞的变化的可变速度。 在一个实例中，通过将细胞包装到毛细管或提供光学等效的360度观察通道的任何其它线性基质中，使细胞在毛细管内是固定的，但毛细管被平移并机械地旋转 可变运动光学层析成像圆筒。 在计算机程序的控制下，毛细管运动可以有利地以起始和停止的方式被控制并且以任何运动间隔的任何速度平移和旋转。 因此，存在利用该受控运动能力的光学层析成像系统的几种配置。 另外，描述了使用偏振滤光器和相位板来减少光散射和衍射背景噪声。

公开(公告)号：US07080324B1

公开(公告)日：2006-07-18

申请号：US09686588

申请日：2000-10-11

Applicant: Alan C. Nelson ,  Janice G. Ryles

Inventor： Alan C. Nelson ,  Janice G. Ryles

IPC: G06F3/00

CPC classification number: G06F3/04847 ,  Y10S715/974

Abstract: A variable control displayed through a graphical user interface and linked to a control element. The variable control has graphical representations of readily identifiable and easy to use controls. These controls include at least one text box to display a value. The displayed value can be set by default, or to show the current state of the control element. The variable control also includes at least one range box for assigning a range unit that corresponds to the text box. The variable control also includes a slider control that is linked to the text box and is used to make coarse adjustments to the value displayed in the text box. A spinner is also linked to the text box and is used to make fine adjustments to the displayed value. In a preferred embodiment, the user can select a digit or several digits within the displayed value and adjust only the user-selected digit or digits. Also in a preferred embodiment, the range box automatically updates the range unit when the displayed value is adjusted beyond a predetermined range limit.

Abstract translation: 通过图形用户界面显示并链接到控制元素的变量控件。 变量控件具有易于识别和易于使用的控件的图形表示。 这些控件至少包含一个显示值的文本框。 显示值可以默认设置，也可以显示控制元素的当前状态。 变量控制还包括用于分配对应于文本框的范围单元的至少一个范围框。 变量控件还包括链接到文本框的滑块控件，用于对文本框中显示的值进行粗调。 旋转器也链接到文本框，用于对显示的值进行微调。 在优选实施例中，用户可以在所显示的值内选择一位或多位数字，并且仅调整用户选择的数字或数字。 同样在优选实施例中，当显示值被调整超过预定范围限制时，范围框自动更新范围单位。