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    Repertoire determination of a lymphocyte B population
    1.
    发明申请
    Repertoire determination of a lymphocyte B population 审中-公开
    汇编确定淋巴细胞B群体

    公开(公告)号：US20080166718A1

    公开(公告)日：2008-07-10

    申请号：US11822280

    申请日：2007-07-03

    申请人： Annick Lim ,  Brigitte Lemercier ,  Philippe Kourilsky ,  Francois Huetz

    发明人： Annick Lim ,  Brigitte Lemercier ,  Philippe Kourilsky ,  Francois Huetz

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6876 ,  C07K16/00 ,  C07K2317/21 ,  C07K2317/56 ,  C12Q2600/156 ,  C12Q2600/158

    摘要： The present invention provides a process for determining the quantitative and qualitative profile of the repertoire of a given type of an immunoglobulin (Ig) heavy chain expressed by a B lymphocyte population present in a tissue sample, and kits and uses thereof. It also provides a set of VH forward primers associated with a CH reverse primer, which are respectively capable of specifically hybridizing in stringent conditions with the nucleic acids encoding the variable segments of Ig heavy chains and with the constant segment of a given type of Ig heavy chain, such as preferably an IgM, IgG, IgE or IgA heavy chain. Methods for the in vitro diagnosis of a condition associated with an abnormal expression of the repertoire of a given type of Ig heavy chain, and for the in vitro follow-up of a treatment of such a condition, are also included herein.

    摘要翻译： 本发明提供了确定由组织样品中存在的B淋巴细胞群表达的给定类型的免疫球蛋白（Ig）重链的所有组成成分的定量和定性分析的方法及其试剂盒和用途。 它还提供了一组与CH反向引物相关的VH正向引物，其分别能够在严格条件下与编码Ig重链的可变区的核酸以及给定类型的Ig重量的恒定区特异性杂交 链，例如优选IgM，IgG，IgE或IgA重链。 用于体外诊断与给定类型的Ig重链的所有组成成分异常表达有关的病症的方法以及对这种病症的治疗的体外随访。

    Repertoire determination of a lymphocyte b population
    2.
    发明申请
    Repertoire determination of a lymphocyte b population 审中-公开
    汇编确定淋巴细胞b群体

    公开(公告)号：US20070160994A1

    公开(公告)日：2007-07-12

    申请号：US10582613

    申请日：2004-12-15

    申请人： Annick Lim ,  Brigitte Marie-Christine Lemercier ,  Philippe Kourilsky ,  Francois Huetz

    发明人： Annick Lim ,  Brigitte Marie-Christine Lemercier ,  Philippe Kourilsky ,  Francois Huetz

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6881 ,  C12Q2600/156

    摘要： The present invention provides a process for determining the quantitative and qualitative profile of the repertoire of a given type of an immunoglobulin (Ig) heavy chain expressed by a lymphocyte B population present in a tissue sample, and kits and uses thereof. It also provides a set of VII forward primers associated with a CII reverse primer, which are respectively capable of specifically hybridizing in stringent conditions with the nucleic acids encoding the variable segments of Ig heavy chains and with the constant segment of a given type of Ig heavy chain, such as preferably IgM, IgG, IgE or IgA heavy chain. Methods for the in vitrodiagnosis of a condition associated with an abnormal expression of the repertoire of a given type of Ig heavy chain, and for the in vitro follow-up of a treatment of such a condition, are also comprised herein.

    摘要翻译： 本发明提供了确定由组织样品中存在的淋巴细胞B群体表达的给定类型的免疫球蛋白（Ig）重链的所有组成成分的定量和定性分析的方法及其试剂盒和用途。 它还提供了与CII反向引物相关的一组VII正向引物，其能够在严格条件下与编码Ig重链的可变区的核酸以及给定类型的Ig重量的恒定区特异性杂交 链，例如优选IgM，IgG，IgE或IgA重链。 与特定类型的Ig重链的所有组成成分异常表达相关的病症的体外诊断方法以及对这种病症的治疗的体外随访也包括在本文中。

    Process for determining the quantity of a DNA fragment of interest by a method of enzymatic amplification of DNA
    6.
    发明授权
    Process for determining the quantity of a DNA fragment of interest by a method of enzymatic amplification of DNA 失效
    通过酶扩增DNA的方法测定感兴趣的DNA片段的量的方法

    公开(公告)号：US5747246A

    公开(公告)日：1998-05-05

    申请号：US263413

    申请日：1994-06-21

    申请人： Christophe Pannetier ,  Madeleine Cochet ,  Sylvie Darche ,  Philippe Kourilsky

    发明人： Christophe Pannetier ,  Madeleine Cochet ,  Sylvie Darche ,  Philippe Kourilsky

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6851

    摘要： The present invention relates to a process for determining the quantity of a DNA fragment of interest in a sample wherein: 1) a standard DNA fragment can be amplified with the same oligonucleotide primers is added to the sample to be analyzed containing the DNA fragment of interest, the standard DNA fragment and the fragment of interest differing in sequence and/or in size by not more than 10%, 2) the DNA fragment of interest and the standard fragment are coamplified with the same primers, preferably to saturation of the amplification of the DNA fragment of interest, 3) one or more labeled oligonucleotide primer(s), specific for the DNA fragment of interest and the standard fragment and different from the primers of step 2), is/are added to the reaction medium obtained in step 2), so that, after denaturation of the DNA, said primer(s) hybridize(s) with said fragments at a suitable site in order that an elongation with the DNA polymerase generates labeled DNA fragments of different sizes and/or sequences or with different labels according to whether they originate from the DNA fragment of interest or the standard fragment, respectively, and then 4) the initial quantity of DNA fragment of interest is determined as being the product of the initial quantity of standard DNA fragment and the ratio of the quantity of amplified DNA fragment of interest to the quantity of amplified standard DNA fragment.

    摘要翻译： 本发明涉及一种测定样品中感兴趣的DNA片段的量的方法，其中：1）可以用相同的寡核苷酸引物扩增标准DNA片段，将其加入待分析的样品中，其含有感兴趣的DNA片段 ，标准DNA片段和感兴趣的片段在序列和/或大小上不同于10％，2）感兴趣的DNA片段和标准片段用相同的引物共扩增，优选扩增的饱和度 感兴趣的DNA片段，3）在步骤2中获得的反应介质中加入一种或多种针对目的DNA片段特异的标记寡核苷酸引物和与步骤2）的引物不同的标准片段） 2），使得在DNA变性后，所述引物在合适的位点与所述片段杂交，以便DNA聚合酶的延伸产生不同大小的标记的DNA片段 s和/或序列或不同标记，根据它们是否源自目的DNA片段或标准片段，然后4）目的DNA片段的初始量被确定为初始量的 标准DNA片段和目标扩增DNA片段的量与扩增的标准DNA片段的量的比例。

    Altered major histocompatibility complex (MHC) determinant and methods of using the determinant
    10.
    发明授权
    Altered major histocompatibility complex (MHC) determinant and methods of using the determinant 失效
    改变主要组织相容性复合体（MHC）的决定因素和使用决定簇的方法

    公开(公告)号：US6153408A

    公开(公告)日：2000-11-28

    申请号：US370476

    申请日：1995-01-09

    申请人： Jean-Pierre Abastado ,  Estelle Mottez ,  Philippe Kourilsky ,  Armanda Casrouge ,  David Ojcius ,  Yu-Chun Lone

    发明人： Jean-Pierre Abastado ,  Estelle Mottez ,  Philippe Kourilsky ,  Armanda Casrouge ,  David Ojcius ,  Yu-Chun Lone

    IPC分类号： A61K38/00 ,  C07K14/74 ,  C07K19/00 ,  A61K39/385 ,  C12N15/62 ,  C12N15/85

    CPC分类号： C07K14/70539 ,  A61K38/00 ,  C07K2319/00

    摘要： An altered MHC class I determinant comprises .alpha..sub.1, .alpha..sub.2, .alpha..sub.3, .beta..sub.2 -microglobulin (.beta..sub.2 m) polypeptide domains encoded by a mammalian MHC class I locus in which the .alpha..sub.3 domain is covalently linked to the .beta..sub.2 M domain. An altered MHC class II determinant comprises .alpha..sub.1, .alpha..sub.2, .beta..sub.1, and .beta..sub.2 polypeptide domains encoded by a mammalian MHC class II locus, in which the domains are covalently linked to form a polypeptide comprising the .beta..sub.2 -.alpha..sub.2 -.alpha..sub.1 -.beta..sub.1 domains in sequence. The altered MHC class I and class II determinants can be associated with an antigen to elicit an immune response. The invention can be used in the immunization or treatment of diseases such as AIDS, multiple sclerosis, lupus erythematosus, toxic shock or snake bite.

    摘要翻译： 改变的MHC I类决定簇包含由哺乳动物MHC I类基因座编码的α1，α2，α3，β2-微球蛋白（β2m）多肽结构域，其中α3结构域与β2M结构域共价连接。 改变的MHC II类决定簇包含由哺乳动物MHC II类基因座编码的α1，α2，β1和β2多肽结构域，其中所述结构域共价连接以形成包含β2-α2- 1-β1结构域序列。 改变的MHC I类和II类决定簇可以与抗原相关以引发免疫应答。 本发明可用于免疫或治疗AIDS，多发性硬化症，红斑狼疮，中毒性休克或蛇咬伤等疾病。