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16 条记录 (耗时 0.027 秒)

    Hydrophilic, High Protein Binding, Low Fluorescence, Western Blotting Membrane
    1.
    发明申请
    Hydrophilic, High Protein Binding, Low Fluorescence, Western Blotting Membrane 有权
    亲水，高蛋白结合，低荧光，Western印迹膜

    公开(公告)号：US20110045493A1

    公开(公告)日：2011-02-24

    申请号：US12938813

    申请日：2010-11-03

    申请人： Antoni Peters ,  Philip Goddard ,  John Charkoudian ,  Neil Soice ,  Dave Brewster ,  Anja Dedeo

    发明人： Antoni Peters ,  Philip Goddard ,  John Charkoudian ,  Neil Soice ,  Dave Brewster ,  Anja Dedeo

    IPC分类号： G01N33/53

    CPC分类号： G01N33/54393 ,  B01D67/0093 ,  B01D2323/02 ,  B01D2323/30 ,  B01D2325/36 ,  Y10T436/24

    摘要： Hydrophilic membrane particularly suited for blotting applications, preferably Western blotting. A pre-wet hydrophobic membrane substrate, preferably made of PVDF, is contacted with a monomer solution and subjected to a UV-initiated free radical polymerization step to render the substrate permanently hydrophilic. The resulting membrane exhibits low background fluorescence, high protein binding, excellent retention of protein sample spot morphology, and extended dynamic range (high signal-to-noise ratio, enhanced sample detectability). The membrane demonstrates comparable or higher performance in Western blotting applications than conventional nitrocellulose Western blotting membranes, particularly for protein detection at low sample concentrations, and is directly water-wettable, eliminating the need for an alcohol pre-wet step prior to use.

    摘要翻译： 亲水膜特别适用于印迹应用，优选蛋白印迹法。 将优选由PVDF制成的预湿疏水膜基材与单体溶液接触并进行UV引发的自由基聚合步骤以使基材永久地亲水。 所得膜显示低背景荧光，高蛋白结合，优异的蛋白质样品斑点形态保留和扩展的动态范围（高信噪比，增强的样品检测性）。 与常规硝酸纤维素蛋白质印迹膜相比，该膜在Western印迹应用中表现出相当或更高的性能，特别是在低样品浓度下的蛋白质检测，并且是直接水可湿性的，在使用前无需使用酒精预湿步骤。

    Hydrophilic, high protein binding, low fluorescence, western blotting membrane
    2.
    发明授权
    Hydrophilic, high protein binding, low fluorescence, western blotting membrane 有权
    亲水，高蛋白结合，低荧光，蛋白质印迹膜

    公开(公告)号：US08143067B2

    公开(公告)日：2012-03-27

    申请号：US12938813

    申请日：2010-11-03

    申请人： Antoni Peters ,  Philip Goddard ,  John Charkoudian ,  Neil Soice ,  Dave Brewster ,  Anja Dedeo

    发明人： Antoni Peters ,  Philip Goddard ,  John Charkoudian ,  Neil Soice ,  Dave Brewster ,  Anja Dedeo

    IPC分类号： C12Q1/00 ,  G01N33/53 ,  C12P19/26 ,  B01D57/02

    CPC分类号： G01N33/54393 ,  B01D67/0093 ,  B01D2323/02 ,  B01D2323/30 ,  B01D2325/36 ,  Y10T436/24

    摘要： Hydrophilic membrane particularly suited for blotting applications, preferably Western blotting. A pre-wet hydrophobic membrane substrate, preferably made of PVDF, is contacted with a monomer solution and subjected to a UV-initiated free radical polymerization step to render the substrate permanently hydrophilic. The resulting membrane exhibits low background fluorescence, high protein binding, excellent retention of protein sample spot morphology, and extended dynamic range (high signal-to-noise ratio, enhanced sample detectability). The membrane demonstrates comparable or higher performance in Western blotting applications than conventional nitrocellulose Western blotting membranes, particularly for protein detection at low sample concentrations, and is directly water-wettable, eliminating the need for an alcohol pre-wet step prior to use.

    摘要翻译： 亲水膜特别适用于印迹应用，优选蛋白印迹法。 将优选由PVDF制成的预湿疏水膜基材与单体溶液接触并进行UV引发的自由基聚合步骤以使基材永久地亲水。 所得膜显示低背景荧光，高蛋白结合，优异的蛋白质样品斑点形态保留和扩展的动态范围（高信噪比，增强的样品检测性）。 与常规硝酸纤维素蛋白质印迹膜相比，该膜在Western印迹应用中表现出相当或更高的性能，特别是在低样品浓度下的蛋白质检测，并且是直接水可湿性的，在使用前无需使用酒精预湿步骤。

    Hydrophilic, high protein binding, low fluorescence, western blotting membrane
    3.
    发明申请
    Hydrophilic, high protein binding, low fluorescence, western blotting membrane 有权
    亲水，高蛋白结合，低荧光，蛋白质印迹膜

    公开(公告)号：US20100044302A1

    公开(公告)日：2010-02-25

    申请号：US12462635

    申请日：2009-08-06

    申请人： Antoni Peters ,  Philip Goddard ,  John Charkoudian ,  Neil Soice ,  Dave Brewster ,  Anja Dedeo

    发明人： Antoni Peters ,  Philip Goddard ,  John Charkoudian ,  Neil Soice ,  Dave Brewster ,  Anja Dedeo

    IPC分类号： B01D71/40 ,  B01D71/34 ,  B05D3/02

    CPC分类号： G01N33/54393 ,  B01D67/0093 ,  B01D2323/02 ,  B01D2323/30 ,  B01D2325/36 ,  Y10T436/24

    摘要： Hydrophilic membrane particularly suited for blotting applications, preferably Western blotting. A pre-wet hydrophobic membrane substrate, preferably made of PVDF, is contacted with a monomer solution and subjected to a UV-initiated free radical polymerization step to render the substrate permanently hydrophilic. The resulting membrane exhibits low background fluorescence, high protein binding, excellent retention of protein sample spot morphology, and extended dynamic range (high signal-to-noise ratio, enhanced sample detectability). The membrane demonstrates comparable or higher performance in Western blotting applications than conventional nitrocellulose Western blotting membranes, particularly for protein detection at low sample concentrations, and is directly water-wettable, eliminating the need for an alcohol pre-wet step prior to use.

    摘要翻译： 亲水膜特别适用于印迹应用，优选蛋白印迹法。 将优选由PVDF制成的预湿疏水膜基材与单体溶液接触并进行UV引发的自由基聚合步骤以使基材永久地亲水。 所得膜显示低背景荧光，高蛋白结合，优异的蛋白质样品斑点形态保留和扩展的动态范围（高信噪比，增强的样品检测性）。 与常规硝酸纤维素蛋白质印迹膜相比，该膜在Western印迹应用中表现出相当或更高的性能，特别是在低样品浓度下的蛋白质检测，并且是直接水可湿性的，在使用前无需使用酒精预湿步骤。

    Spatially-controlled modified porous membrane
    5.
    发明授权
    Spatially-controlled modified porous membrane 有权
    空间控制改性多孔膜

    公开(公告)号：US07919178B2

    公开(公告)日：2011-04-05

    申请号：US10394627

    申请日：2003-03-21

    申请人： John Charkoudian ,  Philip Goddard

    发明人： John Charkoudian ,  Philip Goddard

    IPC分类号： B32B5/18 ,  B01D71/06

    CPC分类号： B01D67/0088 ,  B01D67/0093 ,  B01D69/02 ,  B01D69/125 ,  B01D2323/30 ,  Y10T428/249955 ,  Y10T428/249958 ,  Y10T428/249978 ,  Y10T428/249991

    摘要： This invention relates to a general method to surface modify porous membranes with a free radical polymerized polymer coating in a layer which is less than the thickness of the membrane. Another object of this invention relates to a general method to surface modify porous membranes with a free radical polymerized polymer coating to a predetermined and controlled depth from a first facial surface. Another object of this invention is to provide a general method to surface modify porous membranes with a free radical polymerized polymer coating into a pattern on one or both facial surfaces, the pattern maintaining its form to a controlled depth into the membrane. Membrane modification is spatially controlled through the use of a polymerization preventing agent (PPA), which prevents modification from occurring in defined spatially volumes of the membrane. The invention relates to porous membranes surface modified by the methods described.

    摘要翻译： 本发明涉及一种在低于膜厚度的层中用自由基聚合的聚合物涂层表面改性多孔膜的一般方法。 本发明的另一个目的涉及一种用自由基聚合的聚合物涂层将多孔膜表面修饰成从第一面部表面到预定和受控深度的一般方法。 本发明的另一个目的是提供一种通过自由基聚合的聚合物涂层将多孔膜表面修饰成一个或两个面部表面上的图案的一般方法，该图案将其形式保持在受控的深度进入膜。 通过使用聚合阻止剂（PPA）在空间上控制膜改性，其防止在限定的空间体积的膜中发生修饰。 本发明涉及通过所述方法表面改性的多孔膜。

    NANOFIBER CONTAINING COMPOSITE MEMBRANE STRUCTURES
    8.
    发明申请
    NANOFIBER CONTAINING COMPOSITE MEMBRANE STRUCTURES 审中-公开
    包含复合膜的NANOFIBER结构

    公开(公告)号：US20130092622A1

    公开(公告)日：2013-04-18

    申请号：US13436043

    申请日：2012-03-30

    申请人： Onur Y. Kas ,  Mikhail Kozlov ,  Gabriel Tkacik ,  David Nhiem ,  Philip Goddard ,  Sherry Ashby Leon

    发明人： Onur Y. Kas ,  Mikhail Kozlov ,  Gabriel Tkacik ,  David Nhiem ,  Philip Goddard ,  Sherry Ashby Leon

    IPC分类号： B01D69/12

    CPC分类号： B01D69/12 ,  B01D71/56 ,  B01D2323/39 ,  B01D2325/02 ,  D01D5/0084

    摘要： A composite liquid filtration platform including a composite filtration medium featuring an electrospun polymeric nanofiber layer collected on a porous membrane. When in use, the porous membrane acts as a prefilter used upstream from the polymeric nanofiber layer to remove particles from a liquid stream flowing through the composite filtration structure. The nanofiber layer, positioned downstream from the porous membrane, is used as the retentive layer for critical filtration to provide biosafety assurance, and is responsible for capturing microorganisms like bacteria, mycoplasma or viruses. The composite liquid filtration platform provided herein exhibits permeability advantages over conventional porous membranes or nanofiber mats spun on coarse non-wovens.

    摘要翻译： 一种复合液体过滤平台，其包括复合过滤介质，其特征在于在多孔膜上收集的电纺聚合物纳米纤维层。 当使用时，多孔膜用作在聚合物纳米纤维层上游使用的预过滤器，以从流过复合过滤结构的液体流中除去颗粒。 位于多孔膜下游的纳米纤维层用作临界过滤的保留层，以提供生物安全保证，并负责捕获细菌，支原体或病毒等微生物。 本文提供的复合液体过滤平台相对于在粗糙无纺布上纺出的常规多孔膜或纳米纤维垫表现出渗透性优点。