公开(公告)号：US20230313278A1

公开(公告)日：2023-10-05

申请号：US18106189

申请日：2023-02-06

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald LEBOFSKY

IPC: C12Q1/6834 ,  C12Q1/6855

CPC classification number: C12Q1/6834 ,  C12Q1/6855 ,  C12Q2565/1015 ,  C12Q2563/137

Abstract: Methods and compositions for attaching cell-specific barcodes without formation of partitions is provided.

公开(公告)号：US20210164036A1

公开(公告)日：2021-06-03

申请号：US17136824

申请日：2020-12-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald LEBOFSKY ,  Jennifer CHEW

IPC: C12Q1/6869 ,  C12Q1/6855 ,  C12Q1/6862 ,  C12Q1/6876 ,  C12Q1/6848 ,  C12Q1/686 ,  C12N15/10 ,  C12N9/12 ,  C12Q1/6806

Abstract: The methods and reagents are provided for barcoding and analysis of DNA samples using partition (e.g., droplet) technology while avoiding performing amplification in droplets.

公开(公告)号：US20230383332A1

公开(公告)日：2023-11-30

申请号：US18203424

申请日：2023-05-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald LEBOFSKY ,  Josh SHINOFF ,  Adam M. McCOY ,  Man CHENG

IPC: C12Q1/6806 ,  C12Q1/6869

CPC classification number: C12Q1/6806 ,  C12Q1/6869 ,  C12N1/06

Abstract: Methods of making and forming partitions in nanovials are provided.

公开(公告)号：US20230235391A1

公开(公告)日：2023-07-27

申请号：US17962338

申请日：2022-10-07

Applicant: Bio-Rad Laboratories, Inc. ,  President and Fellows of Harvard College

Inventor： Ronald LEBOFSKY ,  Jason BUENROSTRO

IPC: C12Q1/6874

CPC classification number: C12Q1/6874 ,  C12Q1/6841

Abstract: Methods and compositions for determining the proximity of two barcoding oligonucleotides (e.g., in a single partition or adjacent on a tissue section) using a determination of the presence of a 9 bp sequence resulting from tagmentation in different nucleic acid fragments linked to different barcoding oligonucleotides is provided.

公开(公告)号：US20210317509A1

公开(公告)日：2021-10-14

申请号：US17236743

申请日：2021-04-21

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald LEBOFSKY ,  Jeremy AGRESTI

IPC: C12Q1/6809 ,  G16B20/00 ,  C12Q1/6869

Abstract: Methods and compositions for maintaining DNA contiguity for sequencing is provided.

公开(公告)号：US20240158847A1

公开(公告)日：2024-05-16

申请号：US18499894

申请日：2023-11-01

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald LEBOFSKY ,  Jennifer CHEW

IPC: C12Q1/6869 ,  C12N9/12 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6848 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q1/6876

CPC classification number: C12Q1/6869 ,  C12N9/1252 ,  C12N15/1003 ,  C12Q1/6806 ,  C12Q1/6848 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q1/6876 ,  C12Q2563/185 ,  C12Y207/07007

Abstract: The methods and reagents are provided for barcoding and analysis of DNA samples using partition (e.g., droplet) technology while avoiding performing amplification in droplets.

公开(公告)号：US20240132953A1

公开(公告)日：2024-04-25

申请号：US18381400

申请日：2023-10-17

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Man CHENG ,  Ronald LEBOFSKY

IPC: C12Q1/6874 ,  C12Q1/6876

CPC classification number: C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/682

Abstract: Methods and compositions for generating sequencing reads by partition of origin. One can introduce a unique molecular identifier and bead-specific barcodes to target nucleic acid fragments and the combination of bead-specific barcode, UMI and fragment can be used to identify when multiple bead-specific barcodes originated in the same partition, allowing for improved deconvolution of partition-based sequence analysis.

公开(公告)号：US20240093268A1

公开(公告)日：2024-03-21

申请号：US18381371

申请日：2023-10-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald LEBOFSKY ,  Jeremy AGRESTI ,  George KARLIN-NEUMANN

IPC: C12Q1/6806 ,  C12Q1/6811 ,  C12Q1/682 ,  C12Q1/6832 ,  C12Q1/6853 ,  C12Q1/686

CPC classification number: C12Q1/6806 ,  C12Q1/6811 ,  C12Q1/682 ,  C12Q1/6832 ,  C12Q1/6853 ,  C12Q1/686

Abstract: Improved multiple displacement amplification (MDA) reagents and methods are provided.

公开(公告)号：US20230392194A1

公开(公告)日：2023-12-07

申请号：US18143268

申请日：2023-05-04

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald LEBOFSKY ,  Jeremy AGRESTI

IPC: C12Q1/6809 ,  G16B20/00 ,  C12Q1/6869

CPC classification number: C12Q1/6809 ,  G16B20/00 ,  C12Q1/6869

Abstract: Methods and compositions for maintaining DNA contiguity for sequencing is provided.

公开(公告)号：US20230012786A1

公开(公告)日：2023-01-19

申请号：US17944758

申请日：2022-09-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald LEBOFSKY ,  Andrew KOHLWAY ,  Jennifer CHEW ,  Zachary BURKETT ,  Man CHENG

IPC: C12Q1/6869 ,  G16B40/10 ,  G16B30/10

Abstract: Comparison of common sequencing reads from sequencing based on partition-based barcoding can be used to improve sequencing results. Increased loading of barcodes per partition can also improve sequencing results.