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公开(公告)号：US10273493B2
公开(公告)日：2019-04-30
申请号：US14531705
申请日：2014-11-03
Applicant: Dow AgroSciences LLC
Inventor： Lakshmi Sastry-Dent , Zehui Cao , Shreedharan Sriram , Steven R. Webb , Debra L. Camper , Navin Elango
Abstract: As disclosed herein, optimal native genomic loci have been identified in monocot plants, such as maize plants, that represent best sites for targeted insertion of exogenous sequences.
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公开(公告)号：US09493844B2
公开(公告)日：2016-11-15
申请号：US14105223
申请日：2013-12-13
Applicant: Dow AgroSciences LLC
Inventor： Lakshmi Sastry-Dent , Matthew Simpson , Zehui Cao , Wei Chen , Ning Zhou , Steven R. Webb
IPC: C12Q1/68
CPC classification number: C12Q1/6858 , A01G24/00 , C12N5/00 , C12N15/1082 , C12N15/66 , C12N15/70 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C40B40/08
Abstract: The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening the intactness or disruption of a targeted genomic loci and optionally for detecting a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.
Abstract translation: 本公开提供了用于检测和鉴定含有精确靶向基因组基因座的植物事件的方法，以及包含这种靶基因组基因座的植物和植物细胞。 该方法可以部署为用于筛选靶基因组基因座的完整性或破坏的高通量过程，并且任选地用于检测目标基因组基因座上的供体DNA多核苷酸插入。 该方法容易适用于鉴定通过使用位点特异性核酸酶产生的靶向方法产生的植物事件。
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公开(公告)号：US10344322B2
公开(公告)日：2019-07-09
申请号：US15249684
申请日：2016-08-29
Applicant: Dow AgroSciences LLC
Inventor： Lakshmi Sastry-Dent , Matthew A. Simpson , Zehui Cao , Wei Chen , Ning Zhou , Steven R. Webb
IPC: C12Q1/68 , C12Q1/6858 , C12Q1/6895 , C12N15/10 , C12N15/66 , C12N15/70 , C40B40/08 , A01G24/00 , C12N5/00
Abstract: The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening the intactness or disruption of a targeted genomic loci and optionally for detecting a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.
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公开(公告)号：US20180371478A1
公开(公告)日：2018-12-27
申请号：US16123326
申请日：2018-09-06
Applicant: DOW AGROSCIENCES LLC
Inventor： Lakshmi Sastry-Dent , Zehui Cao , Shreedharan Sriram , Steven R. Webb , Debra L. Camper , W. Michael Ainley
Abstract: As disclosed herein, optimal native genomic loci of soybean plants have been identified that represent best sites for targeted insertion of exogenous sequences.
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公开(公告)号：US20130130920A1
公开(公告)日：2013-05-23
申请号：US13683442
申请日：2012-11-21
Applicant: Dow AgroSciences LLC
Inventor： Zehui Cao , Stephen Novak , Lakshmi Sastry-Dent , Ning Zhou
IPC: C12Q1/68
CPC classification number: C12Q1/686 , C12Q1/6895
Abstract: A method of analyzing, in chromosomal DNA having a transgene incorporated therein, a DNA flanking region derived from the chromosome which is adjacent to the transgene. Wherein, the DNA flanking region is characterized by isolation and digestion of genomic DNA with a restriction enzyme, ligation of a double stranded adapter to the isolated and digested genomic DNA, a primer extension reaction of the adapter ligated genomic DNA, and the isolation of the primer extension reaction product via a streptavidin-biotin interaction. The DNA flanking region is further characterized via subsequent PCR amplification reactions and DNA sequencing.
Abstract translation: 在其中掺入转基因的染色体DNA中分析衍生自与转基因相邻的染色体的DNA侧翼区的方法。 其中DNA侧翼区的特征在于用限制酶分离和消化基因组DNA，将双链衔接子连接到分离和消化的基因组DNA上，衔接子连接的基因组DNA的引物延伸反应和 通过链霉亲和素 - 生物素相互作用引物延伸反应产物。 通过随后的PCR扩增反应和DNA测序进一步表征DNA侧翼区。
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Patent Agency Ranking
公开(公告)号：US20190024100A1
公开(公告)日：2019-01-24
申请号：US16112200
申请日：2018-08-24
Applicant: Dow AgroSciences LLC
Inventor： Lakshmi Sastry-Dent , Zehui Cao , Shreedharan Sriram , Steven R. Webb , Debra L. Camper
Abstract: As disclosed herein, optimal native genomic loci from maize plants have been identified that represent best sites for targeted insertion of exogenous sequences.
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公开(公告)号：US10077449B2
公开(公告)日：2018-09-18
申请号：US14531710
申请日：2014-11-03
Applicant: Dow AgroSciences LLC
Inventor： Lakshmi Sastry-Dent , Steven L. Evans , Ryan C. Blue , Zehui Cao
IPC: C12N15/82
CPC classification number: C12N15/8213 , C12N15/8209 , C12N15/8212 , C12N15/8275
Abstract: A universal donor polynucleotide is described that can be inserted at targeted locations in plant genomes to facilitate rapid and high throughput integration of a donor molecule within a specific genomic location.
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公开(公告)号：US09909131B2
公开(公告)日：2018-03-06
申请号：US14531748
申请日：2014-11-03
Applicant: Dow AgroSciences LLC
Inventor： Lakshmi Sastry-Dent , Zehui Cao , Shreedharan Sriram , Steven R. Webb , Debra L. Camper , W. Michael Ainley
CPC classification number: C12N15/8213 , A01H5/10 , C12N15/8243 , C12N15/8273 , C12N15/8274 , C12N15/8286
Abstract: As disclosed herein, optimal native genomic loci of soybean plants have been identified that represent best sites for targeted insertion of exogenous sequences.
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公开(公告)号：US09765404B2
公开(公告)日：2017-09-19
申请号：US14475968
申请日：2014-09-03
Applicant: Dow AgroSciences LLC
Inventor： Lakshmi Sastry-Dent , W. Michael Ainley , Jayakumar P. Samuel , Zehui Cao , Liu Y. Shen , Cristie M. Dewes
CPC classification number: C12Q1/6895
Abstract: The present disclosure provides methods for detecting and identifying plant events that contain donor sequences inserted precisely into targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method comprises the steps of amplifying a genomic DNA with a first round of PCR to produce a first amplicon from donor sequences inserted in the reverse orientation, amplifying the first amplicon with a second round of PCR and detecting the presence of the second amplicon, wherein the production of the first and second amplicon indicates the presence of the site specific integration event.
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公开(公告)号：US10093940B2
公开(公告)日：2018-10-09
申请号：US14531739
申请日：2014-11-03
Applicant: Dow AgroSciences LLC
Inventor： Lakshmi Sastry-Dent , Zehui Cao , Shreedharan Sriram , Steven R. Webb , Debra L. Camper
Abstract: As disclosed herein, optimal native genomic loci from maize plants have been identified that represent best sites for targeted insertion of exogenous sequences.
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