公开(公告)号：US20210238539A1

公开(公告)日：2021-08-05

申请号：US17050626

申请日：2019-05-21

Applicant: DSM IP Assets B.V.

Inventor： Christopher Mark FARRELL ,  Lisa Ann LAPRADE ,  Otto Martin LEHMANN ,  Joshua TRUEHEART ,  Bastien Jean Wolfgang CHEVREUX

IPC: C12N1/16 ,  C12N9/90 ,  C12P33/00

Abstract: The present invention is related to an improved method for production of 7-dehydrocholesterol (7-DHC), an important intermediate towards biotechnological production of vitamin D3 or derivatives/metabolites thereof. The invention features modified yeast strains expressing enzymes having improved C-8 sterol isomerase activity leading to increased ratios of 7-DHC in the sterol mix.

公开(公告)号：US20210222222A1

公开(公告)日：2021-07-22

申请号：US17051610

申请日：2019-05-21

Applicant: DSM IP ASSETS B.V.

Inventor： Christopher Mark FARRELL ,  Lisa Ann LAPRADE ,  Otto Martin LEHMANN ,  Joshua TRUEHEART ,  Bastien Jean Wolfgang CHEVREUX

IPC: C12P33/02 ,  C12N9/02 ,  C12N1/16

Abstract: The present invention is related to an improved method for production of 7-dehydrocholesterol (7-DHC), an important intermediate towards biotechnological production of vitamin D3 or derivatives/metabolites thereof. The invention features modified host strains expressing enzymes having improved C-5 sterol 5 desaturase activity and their use in a process for production of vitamin D3 or derivatives and/or metabolites thereof.

公开(公告)号：US20210115412A1

公开(公告)日：2021-04-22

申请号：US17050603

申请日：2019-05-21

Applicant: DSM IP ASSETS B.V.

Inventor： Christopher Mark FARRELL ,  Lisa Ann LAPRADE ,  Otto Martin LEHMANN ,  Joshua TRUEHEART

IPC: C12N9/10 ,  C12P33/00

Abstract: The present invention is related to modified sterol acyltransferase enzymes with improved activity and/or specificity towards acylation of the vitamin D3 precursor 7-dehydrocholesterol (7-DHC) to be used in biotechnological production of vitamin D3. The invention further relates to a yeast strain expressing said modified enzymes and their use in a process for production of vitamin D3 or derivatives and/or metabolites thereof.

公开(公告)号：US20240279706A1

公开(公告)日：2024-08-22

申请号：US18292227

申请日：2021-07-27

Applicant: DSM IP ASSETS B.V.

Inventor： Peter Louis HOUSTON ,  Valmik Kanubhai VYAS ,  Anna SYMBOR-NAGRABSKA ,  Reed Chadbourne DOTEN ,  Christopher Mark FARRELL ,  Ethan LAM

IPC: C12P23/00 ,  C12N1/16 ,  C12N9/10

CPC classification number: C12P23/00 ,  C12N1/16 ,  C12N9/1029

Abstract: The present invention is related to a novel process for production of retinyl acetate in a host cell, particularly oleaginous yeast such as e.g. Yarrowia, wherein the product purity could be increased with reduction of unwanted side-products. Particularly, the novel process comprises fermentation in the presence of ethanol, such as e.g. in a fed-batch fermentation process. Such process is especially useful in a biotechnological process for production of vitamin A.

公开(公告)号：US20220010291A1

公开(公告)日：2022-01-13

申请号：US17293800

申请日：2019-11-11

Applicant: DSM IP ASSETS B.V.

Inventor： Christopher Mark FARRELL ,  Sébastien Eric POTOT ,  Zoltán PRÁGAI ,  Michael Patrick ANAYA

IPC: C12N9/16 ,  C12P17/16

Abstract: The present invention is related to an improved process for production of vitamin B2 using a genetically engineered host cell comprising a heterologous enzyme with pyridoxal phosphatase activity. Using said modified host cell, the yield of riboflavin production could be increased by at least about 5%.