摘要:
Assays using non-natural bases are described. In one embodiment, the method involves contacting a sample suspected of containing the target nucleic acid with a polymerase and first and second primers; amplifying the target nucleic acid, if present in the sample, by PCR using the first and second primers to generate an amplification product having a double-stranded region and a single-stranded region that comprises the non-natural base; contacting the sample with a reporter comprising a label and a non-natural base that is complementary to the non-natural base of the single-stranded region; annealing at least a portion of the reporter to the single-stranded region of the amplification product; and correlating a signal of the label with the presence of the target nucleic acid in the sample. The invention also provides corresponding kits for use in detecting target nucleic acids in a sample.
摘要:
Assays using non-natural bases are described. In one embodiment, the method involves contacting a sample suspected of containing the target nucleic acid with a polymerase and first and second primers; amplifying the target nucleic acid, if present in the sample, by PCR using the first and second primers to generate an amplification product having a double-stranded region and a single-stranded region that comprises the non-natural base; contacting the sample with a reporter comprising a label and a non-natural base that is complementary to the non-natural base of the single-stranded region; annealing at least a portion of the reporter to the single-stranded region of the amplification product; and correlating a signal of the label with the presence of the target nucleic acid in the sample. The invention also provides corresponding kits for use in detecting target nucleic acids in a sample.
摘要:
Solid support assays using non-standard bases are described. A capture oligonucleotide comprising a molecular recognition sequence is attached to a solid support and hybridized with a target oligonucleotide. In some instances, the molecular recognition sequence includes one or more non-standard bases and hybridizes to a complementary tagging sequence of the target oligonucleotide. In other instances, incorporation of a non-standard base (e.g., via PCR or ligation) is used in the assay.
摘要:
Solid support assays using non-standard bases are described. A capture oligonucleotide comprising a molecular recognition sequence is attached to a solid support and hybridized with a target oligonucleotide. In some instances, the molecular recognition sequence includes one or more non-standard bases and hybridizes to a complementary tagging sequence of the target oligonucleotide. In other instances, incorporation of a non-standard base (e.g., via PCR or ligation) is used in the assay.
摘要:
Solid support assays using non-standard bases are described. A capture oligonucleotide comprising a molecular recognition sequence is attached to a solid support and hybridized with a target. In some instances, the molecular recognition sequence includes one or more non-standard bases and hybridizes to a complementary tagging sequence of the target oligonucleotide. In other instances, incorporation of a non-standard base (e.g., via PCR or ligation) is used in the assay.
摘要:
The present invention provides methods, kits and compositions for high fidelity nucleic acid amplification reactions of target nucleic acids such that specific amplification products that incorporate non-standard bases are produced. The amplification reactions can either be linear or exponential. In some embodiments, the fidelity of the amplification reaction is high enough such that a majority of the amplification products maintain non-standard bases at specific sites designated by the user after a specified number of amplification cycles. In some embodiments, the ratio of non-standard nucleoside triphosphates is greater than the amount of standard nucleoside triphosphates initially present in the reaction mixture.
摘要:
Devices, systems and methods for fabricating improved ceramic composite turbine shrouds with abradable seals are disclosed. A high temperature seal with an abradable coating is provided. The seal comprises a three-dimensional, high-density woven composite base structure recessed below the abradable coating, with loops incorporated in and protruding from the base structure. The abradable coating is integrally attached to the base structure via the loops. Additionally, a graded density seal is provided with a three-dimensional, low-density woven composite structure. The low-density structure is abradable. A three-dimensional, high-density woven composite base structure is recessed below the low-density structure, with the low-density structure integrally attached to the base structure via weaving.
摘要:
A method and system for administering the hedging of an employee deferred compensation plan, e.g., which employs hedging of NQDC plan liabilities using total return swaps and/or put and call options and/or forwards, for tax purposes. A plan coordinator coordinates a transfer of information between an employer/plan sponsor, a plan administrator that communicates with the employees/plan participants, and a balance sheet provider. The plan coordinator receives and reconciles data and then calculates and modifies relevant information for tax purposes and use in reports that are provided to the plan sponsor and balance sheet provider. Calculations and modifications for tax purposes allow the plan sponsor to reap tax benefits from the NQDC plan swap hedge. The reports include an upcoming transactions report, which indicates new compensation deferrals, reallocations of previously deferred compensation among specified indices, and withdrawals. A NQDC plan swap hedge re-weighting request report is prepared to notify the balance sheet provider of necessary changes to the NQDC plan swap hedge. Deferred compensation plan details and summary reports are also provided to show the plan information in both individual form and aggregated plan form. Moreover, swap details/summary reports, a put and call option details report, and a forward details report may be provided which indicates future payments that the balance sheet provider must pay the plan sponsor under the swap and/or option and/or forward hedge, as well as hedge instrument information in both in disaggregated individual form and aggregated form. A deferred compensation exposure/swap position summary report is based on differences between deferred compensation liabilities and NQDC plan swap hedges, which represent the plan sponsor's net risk or exposure.
摘要:
A method for compressing digital image data with greyscale values comprises making at least three predictions of the greyscale value of each pixel and comparing the predicted values with the actual value of the pixel. The difference values between the respective predicted values and the actual value of each pixel is computed, and the median difference value of the three difference values is selected for bit coding. Bit coding is carried out by using variable length bit codes, the shortest length bit code being assigned to the most frequently occurring median difference value. The coded values of the median differences are bit packed, and in the case of a pixel where the absolute median difference value exceeds a predetermined value, the actual pixel value is bit packed.
摘要:
A water absorbing particulate polymer material wherein the polymer material when in dry powder form has a particle size of about 50 .mu.m to about 500 .mu.m and wherein the particles consist of internally bonded finer particles which finer particles have a size below 50 .mu.m. The process of using the water absorbing polymer, material to convert a wet sticky mass of inorganic particulate such as coal fines into a crumbly or flowable solid. A process using a water absorbing particulate polymer material to convert a wet sticky mass of organic particulate material into a crumbly or flowable solid wherein the organic matter can be, for example, food waste or sewage.