Nucleic acid amplification using non-standard bases
    1.
    发明申请
    Nucleic acid amplification using non-standard bases 有权
    使用非标准碱的核酸扩增

    公开(公告)号:US20070105099A1

    公开(公告)日:2007-05-10

    申请号:US10542464

    申请日:2004-01-17

    IPC分类号: C12Q1/68 C12P19/34

    摘要: The present invention provides methods, kits and compositions for high fidelity nucleic acid amplification reactions of target nucleic acids such that specific amplification products that incorporate non-standard bases are produced. The amplification reactions can either be linear or exponential. In some embodiments, the fidelity of the amplification reaction is high enough such that a majority of the amplification products maintain non-standard bases at specific sites designated by the user after a specified number of amplification cycles. In some embodiments, the ratio of non-standard nucleoside triphosphates is greater than the amount of standard nucleoside triphosphates initially present in the reaction mixture.

    摘要翻译: 本发明提供用于靶核酸的高保真度核酸扩增反应的方法,试剂盒和组合物,从而产生包含非标准碱基的特异性扩增产物。 扩增反应可以是线性的或指数的。 在一些实施方案中,扩增反应的保真度足够高,使得大部分扩增产物在指定数量的扩增循环后由用户指定的特定位点维持非标准碱基。 在一些实施方案中,非标准核苷三磷酸的比例大于最初存在于反应混合物中的标准核苷三磷酸的量。

    Materials and methods for the detection of severe acute respiratory syndrome virus (SARS)
    2.
    发明申请
    Materials and methods for the detection of severe acute respiratory syndrome virus (SARS) 审中-公开
    检测严重急性呼吸综合征病毒(SARS)的材料和方法

    公开(公告)号:US20070059686A1

    公开(公告)日:2007-03-15

    申请号:US11494820

    申请日:2006-07-28

    IPC分类号: C12Q1/70 C12Q1/68

    CPC分类号: C12Q1/701 C12Q2525/101

    摘要: Disclosed are methods and kits for identifying a virus in a sample, which may include a coronavirus capable of causing Severe Acute Respiratory Syndrome (“SARS”) or SARS-like symptoms. The virus may be a SARS-Associated Coronavirus (“SARS-CoV”). Typically, the methods may include reacting a mixture that includes, in addition to nucleic acid isolated from the sample, at least one oligonucleotide capable of specifically hybridizing to SARS-CoV nucleic acid where the oligonucleotide includes at least one non-natural base. In addition, the mixture may include control nucleic acid.

    摘要翻译: 公开了用于鉴定样品中的病毒的方法和试剂盒,其可以包括能够引起严重急性呼吸综合征(SARS)或SARS样症状的冠状病毒。 病毒可能是SARS相关冠状病毒(“SARS-CoV”)。 通常,所述方法可以包括使除了从样品分离的核酸之外的至少一种能够与SARS-CoV核​​酸特异性杂交的寡核苷酸的混合物反应,其中寡核苷酸包括至少一个非天然碱基。 此外,混合物可以包括对照核酸。