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    PRODUCTION METHOD FOR INTESTINAL EPITHELIAL CELLS AND UTILIZATION THEREOF
    1.
    发明申请
    PRODUCTION METHOD FOR INTESTINAL EPITHELIAL CELLS AND UTILIZATION THEREOF 有权

    公开(公告)号：US20230126568A1

    公开(公告)日：2023-04-27

    申请号：US18146198

    申请日：2022-12-23

    Applicant: FUJIFILM Corporation

    Inventor： Yuki IMAKURA ,  Shinji MIMA ,  Izumi OGURA ,  Nao YAMAZAKI

    IPC: C12N5/071

    Abstract: An object of the present invention is to provide a method of producing an intestinal epithelial cell, which has a large number of cells per area and a high accuracy of kinetic prediction for a CYP3A4 substrate drug such as midazolam, by inducing the differentiation of a pluripotent stem cell, as well as the intestinal epithelial cell, a cell sheet, an evaluation method for a test substance, a screening kit for a test substance, and a cell preparation. According to the present invention, there is provided a production method for an intestinal epithelial cell, including a first differentiation step of differentiating a pluripotent stem cell into an intestinal stem cell, a proliferation step of proliferating the intestinal stem cell obtained in the differentiation step, and a second differentiation step of differentiating the intestinal stem cell obtained in the proliferation step into an intestinal epithelial cell, in which the proliferation step is a step of bringing the intestinal stem cell into a specific state.

    BLOOD VESSEL MODEL
    2.
    发明申请
    BLOOD VESSEL MODEL 审中-公开

    公开(公告)号：US20180356399A1

    公开(公告)日：2018-12-13

    申请号：US15618151

    申请日：2017-06-09

    Applicant: FUJIFILM Corporation ,  The General Hospital Corporation

    Inventor： Koju ITO ,  Chihaya KAKINUMA ,  Shinji MIMA ,  Daichi HIKIMOTO ,  Craig M Neville ,  Cathryn A Sundback

    IPC: G01N33/50

    CPC classification number: G01N33/5088 ,  G01N33/5029 ,  G01N33/5064

    Abstract: The present disclosure provides a blood vessel model including: a pair of channel members, mutually opposing each other, each of which includes an opposing face in which a respective microchannel is formed; and a porous membrane that includes plural through-holes penetrating in a thickness direction, that is disposed between the opposing faces of the pair of channel members, and that partitions between the microchannels, wherein the porous membrane is provided with a vascular endothelial cell layer so as to cover one face facing one of the microchannels, an average opening diameter of the through-holes is from 1 μm to 20 μm, and an opening coverage ratio of the through-holes is from 30% to 70%.

    CELL PRESERVATION OR TRANSPORTATION INSTRUMENT AND CELL TRANSPORATION METHOD
    4.
    发明申请
    CELL PRESERVATION OR TRANSPORTATION INSTRUMENT AND CELL TRANSPORATION METHOD 有权

    公开(公告)号：US20220267704A1

    公开(公告)日：2022-08-25

    申请号：US17742123

    申请日：2022-05-11

    Applicant: FUJIFILM Corporation

    Inventor： Izumi OGURA ,  Keiji SHIGESADA ,  Shinji MIMA ,  Yuki IMAKURA ,  Shun GOTO ,  Nao YAMAZAKI

    IPC: C12M1/04 ,  C12M1/00 ,  C12M1/12

    Abstract: An object of the present invention is to provide a transportation instrument and a transportation method that enable transportation by using a cell culture container as it is. Further, an object of the present invention is to provide a transportation instrument and a transportation method that enable supply of oxygen required for respiration of cells. Further, an object of the present invention is to provide a transportation instrument and a transportation method that enable suppression of migration of a culture medium into and out of cells caused by the influence of an increase in internal pressure with respect to the temperature during transportation. According to the present invention, provided is a cell preservation or transportation instrument including a plurality of cell storage containers, a plate which has a plurality of recesses for holding the plurality of cell storage containers therein, and a flexible material sheet which seals upper opening portions of the plurality of cell storage containers and upper side wall portions formed by side walls of the recesses of the plate and allows ventilation between an inside and an outside of the plurality of cell storage containers.

    METHOD FOR PRODUCING CELL LAMINATE
    6.
    发明申请
    METHOD FOR PRODUCING CELL LAMINATE 审中-公开

    公开(公告)号：US20200095531A1

    公开(公告)日：2020-03-26

    申请号：US16695073

    申请日：2019-11-25

    Applicant: FUJIFILM CORPORATION

    Inventor： Masafumi NISHINO ,  Koju ITO ,  Shinji MIMA ,  Hayato MIYOSHI

    IPC: C12M1/12 ,  C12N5/071

    Abstract: Provided is a method for producing a cell laminate including cell layers on both surfaces of a porous membrane, using a vessel having a bottom portion and a side wall portion standing from a periphery of the bottom portion, the porous membrane, and a holding member configured to hold the porous membrane such that the porous membrane faces an inner bottom surface of the vessel and is held at a position that does not contact the inner bottom surface, the method including culturing first cells in a liquid medium that contacts the inner bottom surface of the vessel and a surface of the porous membrane, in a state in which the porous membrane is held, by the holding member, at a position that does not contact the inner bottom surface of the vessel so as to face the inner bottom surface, and in which the bottom portion of the vessel is positioned at the upper side while the porous membrane is positioned at the lower side in a direction of gravity; and culturing the first cells at a lower surface of the porous membrane and culturing second cells at an upper surface of the porous membrane, in a state in which the porous membrane is held, by the holding member, at a position that does not contact the inner bottom surface of the vessel so as to face the inner bottom surface, and in which the bottom portion of the vessel is positioned at the lower side while the porous membrane is positioned at the upper side in the direction of gravity.

    METHOD FOR PRODUCING INTESTINAL EPITHELIAL CELL AND INTESTINAL EPITHELIAL CELL
    7.
    发明申请
    METHOD FOR PRODUCING INTESTINAL EPITHELIAL CELL AND INTESTINAL EPITHELIAL CELL 有权

    公开(公告)号：US20210147808A1

    公开(公告)日：2021-05-20

    申请号：US17158797

    申请日：2021-01-26

    Applicant: FUJIFILM Corporation

    Inventor： Shinji MIMA ,  Yuki IMAKURA ,  Izumi OGURA

    IPC: C12N5/071

    Abstract: An object of the present invention is to provide a method for producing an intestinal epithelial cell in which the barrier function is maintained while the differentiation of a pluripotent stem cell into a liver cell is suppressed and an intestinal epithelial cell in which the barrier function is maintained while the differentiation into a liver cell is suppressed. According to the present invention, provided is the method for producing an intestinal epithelial cell, including a step 1 of differentiating a pluripotent stem cell into an intestinal stem cell and a step 2 of differentiating the intestinal stem cell obtained in the step 1 into an intestinal epithelial cell in a presence of one or more selected from the group consisting of a MEK1 inhibitor, a DNA methylation inhibitor, and a TGFβ receptor inhibitor, and EGF, in which during the step 2, a cell under differentiation is replated one or more times at the predetermined timing which is defined in the present specification.

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