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公开(公告)号：US11668638B2
公开(公告)日：2023-06-06
申请号：US17650033
申请日：2022-02-04
Applicant: Georgia Tech Research Corporation
Inventor： Todd Sulchek , Alexander Alexeev , Bushra Tasadduq
CPC classification number: G01N15/1056 , B01L3/502761 , B01L3/502776 , G01N1/34 , G01N33/5005 , B01L2200/0652 , B01L2200/0668 , B01L2300/0877 , B01L2300/16 , B01L2400/086 , G01N2015/1006 , G01N2015/1081
Abstract: A microchannel for processing cells by compression of the cells including an inlet, ridges and an outlet. Each ridge including a compressive surface and a cell adhesion entity. The outlet configured to remove at least one of a first portion of the cells and a second portion of the cells from the microchannel. Each ridge oriented at an angle of from 25 degrees to 70 degrees relative to a center axis of the microchannel. The cell adhesion entity configured such that the first portion of the cells has a first adhesion property relative to the cell adhesion entity to follow a first trajectory through the microchannel. The cell adhesion entity further configured such that the second portion of the cells has a second adhesion property relative to the cell adhesion entity to follow a second trajectory through the microchannel. The first trajectory is different from the second trajectory.
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公开(公告)号：US11925934B2
公开(公告)日：2024-03-12
申请号：US16918349
申请日：2020-07-01
Applicant: GEORGIA TECH RESEARCH CORPORATION
Inventor： Todd Sulchek , Alexander Alexeev , Gonghao Wang
CPC classification number: B01L3/502761 , G01N15/0272 , G01N33/48 , G01N33/5005 , B01L3/502776 , B01L2200/0652 , B01L2300/0816 , B01L2300/0864 , B01L2400/086 , G01N15/01 , G01N2015/0288 , G01N33/491
Abstract: Microfluidic devices for cell sorting or cell fractionation are disclosed. A microfluidic device can comprise one or more inlets, a first wall and a second wall, and two or more outlets. The first and second walls can be substantially planar to each other and the first wall having can have a plurality of ridges protruding from the first wall and defining a compression gap between the ridge and a surface of the second wall. The microfluidic device can also be a cell sorting device for sorting a plurality of cells based on one or more biophysical cellular properties including size, elasticity, viscosity, and/or viscoelasticity wherein the cells are subjected to one or more compressions due to the compression gap. Also disclosed are methods for cell sorting based on a variety of biophysical cellular properties.
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公开(公告)号：US11268892B2
公开(公告)日：2022-03-08
申请号：US16348520
申请日：2017-11-08
Applicant: Georgia Tech Research Corporation
Inventor： Todd Sulchek , Alexander Alexeev , Bushra Tasadduq
Abstract: Embodiments of the present disclosure can include a method comprising: providing a plurality of cells to a microchannel, the microchannel coated in at least one cell adhesion entity and comprising a compressive surface and a first outlet, the compressive surface defining a compression gap, flowing the plurality of cells through the microchannel, wherein the flowing comprises: compressing the plurality of cells underneath the compressive surface; and exposing the plurality of cells to the at least one cell adhesion entity, wherein the exposing causes a first portion of the cells having a first adhesion property to temporarily bind to the cell adhesion entity; and collecting the first portion of cells at the first outlet; wherein the compression gap has a height of from 75% to 95% an average diameter of the plurality of cells.
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公开(公告)号：US20220228965A1
公开(公告)日：2022-07-21
申请号：US17650033
申请日：2022-02-04
Applicant: Georgia Tech Research Corporation
Inventor： Todd Sulchek , Alexander Alexeev , Bushra Tasadduq
Abstract: A microchannel for processing cells by compression of the cells including an inlet, ridges and an outlet. Each ridge including a compressive surface and a cell adhesion entity. The outlet configured to remove at least one of a first portion of the cells and a second portion of the cells from the microchannel. Each ridge oriented at an angle of from 25 degrees to 70 degrees relative to a center axis of the microchannel. The cell adhesion entity configured such that the first portion of the cells has a first adhesion property relative to the cell adhesion entity to follow a first trajectory through the microchannel. The cell adhesion entity further configured such that the second portion of the cells has a second adhesion property relative to the cell adhesion entity to follow a second trajectory through the microchannel. The first trajectory is different from the second trajectory.
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Patent Agency Ranking
公开(公告)号：US20200330990A1
公开(公告)日：2020-10-22
申请号：US16918349
申请日：2020-07-01
Applicant: GEORGIA TECH RESEARCH CORPORATION
Inventor： Todd Sulchek , Alexander Alexeev , Gonghao Wang
Abstract: Microfluidic devices for cell sorting or cell fractionation are disclosed. A microfluidic device can comprise one or more inlets, a first wall and a second wall, and two or more outlets. The first and second walls can be substantially planar to each other and the first wall having can have a plurality of ridges protruding from the first wall and defining a compression gap between the ridge and a surface of the second wall. The microfluidic device can also be a cell sorting device for sorting a plurality of cells based on one or more biophysical cellular properties including size, elasticity, viscosity, and/or viscoelasticity wherein the cells are subjected to one or more compressions due to the compression gap. Also disclosed are methods for cell sorting based on a variety of biophysical cellular properties.
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公开(公告)号：US20220062904A1
公开(公告)日：2022-03-03
申请号：US17524168
申请日：2021-11-11
Applicant: Georgia Tech Research Corporation
Inventor： Todd Sulchek , Alexander Alexeev , Anna Liu
Abstract: Embodiments of the present disclosure can include a method for convective intracellular delivery including providing cells and molecules to a microchannel having compressive surfaces, wherein the compressive surfaces define compression gaps having a height of from 20 and 80% of the average cell diameter, and a plurality of relaxation spaces disposed between the compressive surfaces, flowing the cell medium through the microchannel, wherein as the cell medium flows through the microchannel, the plurality of cells undergo a convective intracellular delivery process comprising: compressing the plurality of cells, wherein the compressing causes the plurality of cells to undergo a loss in intracellular volume Vloss, and passing the plurality of cells to a first relaxation space, wherein the plurality of cells undergo a gain in volume Vgain and absorb a portion of the plurality of molecules.
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公开(公告)号：US11198127B2
公开(公告)日：2021-12-14
申请号：US16348170
申请日：2017-11-08
Applicant: Georgia Tech Research Corporation
Inventor： Todd Sulchek , Alexander Alexeev , Anna Liu
Abstract: Embodiments of the present disclosure can include a method for convective intracellular delivery including providing cells and molecules to a microchannel having compressive surfaces, wherein the compressive surfaces define compression gaps having a height of from 20 and 80% of the average cell diameter; and a plurality of relaxation spaces disposed between the compressive surfaces; flowing the cell medium through the microchannel, wherein as the cell medium flows through the microchannel, the plurality of cells undergo a convective intracellular delivery process comprising: compressing the plurality of cells, wherein the compressing causes the plurality of cells to undergo a loss in intracellular volume (Vloss); and passing the plurality of cells to a first relaxation space, wherein the plurality of cells undergo a gain in volume (Vgain) and absorb a portion of the plurality of molecules.
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公开(公告)号：US10717084B2
公开(公告)日：2020-07-21
申请号：US15774684
申请日：2016-11-09
Applicant: GEORGIA TECH RESEARCH CORPORATION
Inventor： Todd Sulchek , Alexander Alexeev , Gonghao Wang
Abstract: Microfluidic devices for cell sorting or cell fractionation are disclosed. A microfluidic device can comprise one or more inlets, a first wall and a second wall, and two or more outlets. The first and second walls can be substantially planar to each other and the first wall having can have a plurality of ridges protruding from the first wall and defining a compression gap between the ridge and a surface of the second wall. The microfluidic device can also be a cell sorting device for sorting a plurality of cells based on one or more biophysical cellular properties including size, elasticity, viscosity, and/or viscoelasticity wherein the cells are subjected to one or more compressions due to the compression gap. Also disclosed are methods for cell sorting based on a variety of biophysical cellular properties.
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公开(公告)号：US20190262835A1
公开(公告)日：2019-08-29
申请号：US16348170
申请日：2017-11-08
Applicant: Georgia Tech Research Corporation
Inventor： Todd Sulchek , Alexander Alexeev , Anna Liu
Abstract: Embodiments of the present disclosure can include a method for convective intracellular delivery including providing cells and molecules to a microchannel having compressive surfaces, wherein the compressive surfaces define compression gaps having a height of from 20 and 80% of the average cell diameter; and a plurality of relaxation spaces disposed between the compressive surfaces; flowing the cell medium through the microchannel, wherein as the cell medium flows through the microchannel, the plurality of cells undergo a convective intracellular delivery process comprising: compressing the plurality of cells, wherein the compressing causes the plurality of cells to undergo a loss in intracellular volume (Vloss); and passing the plurality of cells to a first relaxation space, wherein the plurality of cells undergo a gain in volume (Vgain) and absorb a portion of the plurality of molecules.
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