公开(公告)号：US09766216B2

公开(公告)日：2017-09-19

申请号：US10821657

申请日：2004-04-08

申请人： H. Garrett Wada ,  Irina G. Kazakova ,  Yutaka Miki ,  Toshinari Ohashi ,  Futoshi Kanke

发明人： H. Garrett Wada ,  Irina G. Kazakova ,  Yutaka Miki ,  Toshinari Ohashi ,  Futoshi Kanke

IPC分类号： C12Q1/68 ,  G01N30/86 ,  G01N33/53 ,  G01N33/538 ,  G01N33/561 ,  B01L3/00 ,  G01N30/02 ,  G01N30/34

CPC分类号： G01N30/8644 ,  B01L3/5027 ,  B01L3/502753 ,  B01L2300/0816 ,  B01L2400/0415 ,  B01L2400/0487 ,  G01N30/02 ,  G01N30/34 ,  G01N33/5306 ,  G01N33/538 ,  G01N33/561 ,  B01D15/34

摘要： This invention provides methods and compositions, e.g., to reduce interference from non-specific binding sample constituents in a migration shift assay. Interference due to non-specific binding of sample constituents to an affinity substance (e.g., an affinity molecule or a conjugate of an affinity molecule and a charged carrier molecule) is prevented by, e.g., binding the constituents to charged polymers such as heparin sulfate.The present invention also provides methods to concentrate an analyte of interest with high concentration and to detect the analyte with high sensitivity, and further to optimize the reaction conditions for easily concentrating the analyte. Such objects of the present invention are attained, for example, by concentrating a complex of the analyte and a conjugate which is formed by contacting the analyte in a sample with an affinity molecule bound to a charged carrier molecule such as DNA.

公开(公告)号：US20110024296A1

公开(公告)日：2011-02-03

申请号：US12902006

申请日：2010-10-11

申请人： Charles Park ,  Persefoni Kechagia ,  Michael Spaid ,  Morten Jensen ,  Irina G. Kazakova ,  Josh Molho

发明人： Charles Park ,  Persefoni Kechagia ,  Michael Spaid ,  Morten Jensen ,  Irina G. Kazakova ,  Josh Molho

IPC分类号： G01N27/447

CPC分类号： B01L3/502753 ,  B01L3/502715 ,  B01L3/502746 ,  B01L2200/0673 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2400/0415 ,  B01L2400/084 ,  G01N27/44743 ,  G01N27/44773 ,  G01N27/44791

摘要： This invention provides methods and systems for injection of analytes into a separation channel for resolution and detection. Samples can be preconditioned and concentrated by isotachophoresis (ITP) before the injection is triggered by a detected voltage event. Separation of analytes from other sample constituents can be enhanced using skewing channel ITP.

摘要翻译： 本发明提供了用于将分析物注入到用于分辨和检测的分离通道中的方法和系统。 样品可以通过等速电泳（ITP）进行预处理和浓缩，然后通过检测到的电压事件触发注射。 使用偏斜通道ITP可以提高分析物与其他样品成分的分离。

公开(公告)号：US09539574B2

公开(公告)日：2017-01-10

申请号：US12902006

申请日：2010-10-11

申请人： Charles Park ,  Persefoni Kechagia ,  Michael Spaid ,  Morten Jensen ,  Irina G. Kazakova ,  Josh Molho

发明人： Charles Park ,  Persefoni Kechagia ,  Michael Spaid ,  Morten Jensen ,  Irina G. Kazakova ,  Josh Molho

IPC分类号： G01N27/447 ,  B01L3/00

CPC分类号： B01L3/502753 ,  B01L3/502715 ,  B01L3/502746 ,  B01L2200/0673 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2400/0415 ,  B01L2400/084 ,  G01N27/44743 ,  G01N27/44773 ,  G01N27/44791

摘要： This invention provides methods and systems for injection of analytes into a separation channel for resolution and detection. Samples can be preconditioned and concentrated by isotachophoresis (ITP) before the injection is triggered by a detected voltage event. Separation of analytes from other sample constituents can be enhanced using skewing channel ITP.

摘要翻译： 本发明提供了用于将分析物注入到用于分辨和检测的分离通道中的方法和系统。 样品可以通过等速电泳（ITP）进行预处理和浓缩，然后通过检测到的电压事件触发注射。 使用偏斜通道ITP可以提高分析物与其他样品成分的分离。

公开(公告)号：US20090118139A1

公开(公告)日：2009-05-07

申请号：US11899599

申请日：2007-09-05

申请人： Jude A. Dunne ,  Javier A. Farinas ,  Irina G. Kazakova ,  Raphaele Gerber ,  Coleen Hacker ,  Roger Dettloff ,  Esther Huang ,  Thi Ngoc Vy Trinh

发明人： Jude A. Dunne ,  Javier A. Farinas ,  Irina G. Kazakova ,  Raphaele Gerber ,  Coleen Hacker ,  Roger Dettloff ,  Esther Huang ,  Thi Ngoc Vy Trinh

IPC分类号： C40B30/08 ,  C40B40/10 ,  C40B60/12

CPC分类号： C12Q1/485 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0829 ,  B01L2300/0838 ,  B01L2300/14 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12Q1/42 ,  G01N30/88 ,  G01N2500/04 ,  G01N2500/20

摘要： Methods for screening a compound for enzyme inhibition activity include providing at least one sample mixture to a microfluidic device, applying vacuum pressure to the sample mixture, flowing the sample mixture along a microchannel of the microfluidic device, separating at least two components of the sample mixture based upon a net charge difference between the product and at least one other material to produce separated material, detecting at least one of the separated materials, and determining enzyme inhibition activity based on the detection of the separated material. Kits for screening a compound for enzyme inhibition activity include a first multiwell plate having a specific plurality of enzymes disposed within a first plurality of wells and a second multiwell plate having a plurality of enzyme substrates disposed with a second plurality of wells, a phosphate source and a cofactor disposed within each well of the second plate.

摘要翻译： 用于筛选化合物用于酶抑制活性的方法包括向微流体装置提供至少一个样品混合物，向样品混合物施加真空压力，沿着微流体装置的微通道流动样品混合物，分离样品混合物的至少两个组分 基于产物与至少一种其它材料之间的净电荷差异来产生分离的材料，检测至少一种分离的材料，以及基于分离的材料的检测来确定酶抑制活性。 用于筛选化合物用于酶抑制活性的试剂盒包括具有设置在第一多个孔内的特定多个酶的第一多孔板和具有多个设置有第二多个孔的酶底物的第二多孔板，磷酸盐源和 配置在第二板的每个孔内的辅因子。