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    Methods of screening heteromeric T1R2/T1R3 receptors for compounds which modulate sweet taste signaling
    1.
    发明授权
    Methods of screening heteromeric T1R2/T1R3 receptors for compounds which modulate sweet taste signaling 有权
    筛选异源性T1R2 / T1R3受体调节甜味信号的化合物的方法

    公开(公告)号：US08809000B2

    公开(公告)日：2014-08-19

    申请号：US13357110

    申请日：2012-01-24

    Applicant: Jon E. Adler ,  Xiaodong Li ,  Lena Staszewski ,  Hong Xu ,  Fernando Echeverri

    Inventor： Jon E. Adler ,  Xiaodong Li ,  Lena Staszewski ,  Hong Xu ,  Fernando Echeverri

    IPC: G01N33/567 ,  C07K1/00 ,  C07K14/00 ,  C07K17/00

    CPC classification number: G01N33/5041 ,  C07K14/723 ,  G01N33/5008 ,  G01N33/5023 ,  G01N33/74 ,  G01N2333/705 ,  G01N2333/726 ,  G01N2500/00

    Abstract: Newly identified mammalian taste-cell-specific G protein-coupled receptors which function as hetero-oligomeric complexes in the sweet taste transduction pathway, and the genes and cDNA encoding said receptors are described. Specifically, T1R G protein-coupled receptors active in sweet taste signaling as hetero-oligomeric complexes, and the genes and cDNA encoding the same, are described, along with methods for isolating such genes and for isolating and expressing such receptors. Methods for identifying putative taste modulating compounds using such hetero-oligomeric complexes also described, as is a novel surface expression facilitating peptide useful for targeting integral plasma membrane proteins to the surface of a cell.

    Abstract translation: 描述了在甜味转导途径中作为异寡聚复合物起作用的新鉴定的哺乳动物味细胞特异性G蛋白偶联受体，以及编码所述受体的基因和cDNA。 具体来说，描述了作为异寡聚复合物的甜味信号传导中起作用的T1R G蛋白偶联受体，以及编码相同基因和cDNA的方法，以及用于分离这些基因并分离和表达此类受体的方法。 还描述了使用这种异寡​​聚复合物鉴定推定的味觉调节化合物的方法，以及用于靶向细胞表面的整合质膜蛋白的新颖的表达表达促进肽。

    Use of bitter taste receptor to screen for compounds that offset the bitter taste of chlorogenic lactone containing coffee compositions
    4.
    发明授权
    Use of bitter taste receptor to screen for compounds that offset the bitter taste of chlorogenic lactone containing coffee compositions 有权
    使用苦味受体筛选抵消含绿色内酯的咖啡组合物的苦味的化合物

    公开(公告)号：US08318447B2

    公开(公告)日：2012-11-27

    申请号：US13094974

    申请日：2011-04-27

    Applicant: Xiaodong Li ,  Hong Xu ,  Qing Li ,  Huixian Tang

    Inventor： Xiaodong Li ,  Hong Xu ,  Qing Li ,  Huixian Tang

    IPC: G01N33/567 ,  C07K14/705

    CPC classification number: C07K14/705 ,  G01N33/566 ,  G01N2333/726 ,  G01N2500/00 ,  G01N2500/10 ,  Y10S514/974

    Abstract: The present invention relates to the discovery that specific human taste receptors in the T2R taste receptor family respond to particular bitter compounds, i.e., chlorogenic lactone compounds that contribute at least partially to the bitter taste of many coffee beverages. The present invention further relates to the use of these receptors in assays for identifying ligands that modulate the activation of these taste receptors by chlorogenic lactones and related compounds and which may be used as additives and/or removed from foods, beverages and medicinals in order to modify (block) T2R-associated bitter taste. A preferred embodiment is the use of the identified compounds as additives in coffee and coffee-flavored foods, beverages and medicinals.

    Abstract translation: 本发明涉及T2R味觉受体家族中的特定人味受体对特定苦味化合物的反应的发现，即，至少部分地促成许多咖啡饮料的苦味的绿色内酯化合物。 本发明还涉及这些受体在测定中用于鉴定调节绿色内酯和相关化合物来调节这些味觉受体的活化并可以用作添加剂和/或从食品，饮料和药物中去除的配体的用途，以便 修改（阻断）T2R相关的苦味。 优选的实施方案是使用所鉴定的化合物作为咖啡和咖啡风味食品，饮料和药物中的添加剂。

    Methods of Cationic Polymer Detection
    5.
    发明申请
    Methods of Cationic Polymer Detection 有权
    阳离子聚合物检测方法

    公开(公告)号：US20120270328A1

    公开(公告)日：2012-10-25

    申请号：US13119295

    申请日：2010-12-21

    Applicant: Guixi Zhang ,  Sijing Wang ,  Hong Xu ,  Xiaofeng Tang

    Inventor： Guixi Zhang ,  Sijing Wang ,  Hong Xu ,  Xiaofeng Tang

    IPC: G01N21/49

    CPC classification number: G01N21/49

    Abstract: The present invention concerns a method of detecting cationic polymers comprising: obtaining a target water sample containing a cationic polymer; adding a polymer dispersant solution and a phosphate solution to the target water sample, the polymer dispersant solution is comprised of a polymer dispersant with calcium and magnesium hardness and the phosphate solution is comprised of a phosphate; standing the target water sample; and measuring the turbidity of the target water sample; comparing the turbidity of said target water sample with a calibration curve of the turbidity of samples containing known concentrations of cationic polymers to determine the concentration of cationic polymers in said target water sample.

    Abstract translation: 本发明涉及一种检测阳离子聚合物的方法，包括：获得含有阳离子聚合物的目标水样品; 将聚合物分散剂溶液和磷酸盐溶液加入到目标水样品中，聚合物分散剂溶液由具有钙和镁硬度的聚合物分散剂组成，磷酸盐溶液由磷酸盐组成; 站在目标水样上; 并测量目标水样品的浊度; 将所述目标水样品的浊度与含有已知浓度的阳离子聚合物的样品的浊度的校准曲线进行比较，以确定所述目标水样品中阳离子聚合物的浓度。

    In vitro methods of detecting cells sensitive to sweet tastants by detecting expression of hetero-oligomeric T1R2/T1R3 taste receptors
    6.
    发明授权
    In vitro methods of detecting cells sensitive to sweet tastants by detecting expression of hetero-oligomeric T1R2/T1R3 taste receptors 有权
    通过检测异低聚T1R2 / T1R3味觉受体的表达来检测对甜味滋味物敏感的细胞的体外方法

    公开(公告)号：US08206938B2

    公开(公告)日：2012-06-26

    申请号：US13099327

    申请日：2011-05-02

    Applicant: Mark Zoller ,  Xiaodong Li ,  Lena Staszewski ,  Shawn O'Connell ,  Sergey Zozulya ,  Jon Elliot Adler ,  Hong Xu ,  Fernando Echeverri

    Inventor： Mark Zoller ,  Xiaodong Li ,  Lena Staszewski ,  Shawn O'Connell ,  Sergey Zozulya ,  Jon Elliot Adler ,  Hong Xu ,  Fernando Echeverri

    IPC: G01N33/53 ,  G01N33/567 ,  C12Q1/68

    CPC classification number: G01N33/5041 ,  A01K2217/05 ,  C07K14/705 ,  C07K2319/00 ,  C12Q1/6876 ,  C12Q2600/124 ,  C12Q2600/158 ,  G01N33/5008 ,  G01N33/566 ,  G01N2333/4719 ,  G01N2333/705 ,  G01N2333/726 ,  G01N2500/04

    Abstract: The present invention relates to the discovery that the T1R receptors assemble to form functional taste receptors. Particularly, it has been discovered that co-expression of T1R1 and T1R3 results in a taste receptor that responds to umami taste stimuli, including monosodium glutamate. Also, it has been discovered that co-expression of the T1R2 and T1R3 receptors results in a taste receptor that responds to sweet taste stimuli including naturally occurring and artificial sweeteners.Also the present invention relates to the use of hetero-oligomeric taste receptors comprising T1R1/T1R3 and T1R2/T1R3 in assays to identify compounds that respectively respond to umami taste stimuli and sweet taste stimuli.Further, the invention relates to the constitutive of cell lines that stably or transiently co-express a combination of T1R1 and T1R3; or T1R2 and T1R3; under constitutive or inducible conditions. The use of these cells lines in cell-based assays to identify umami and sweet taste modulatory compounds is also provided, particularly high throughput screening assays that detect receptor activity by use of fluorometric imaging.Finally, the invention relates to the discovery that some compounds, e.g., lactisole, inhibit both the activities of human T1R2/T1R3 and T1R1/T1R3 receptors, and accordingly the sweet and umami taste, suggesting that these receptors may be the only sweet and umami receptors.

    Abstract translation: 本发明涉及T1R受体组装形成功能性味觉受体的发现。 特别地，已经发现，T1R1和T1R3的共表达导致对鲜味刺激（包括谷氨酸钠）起反应的味觉感受器。 此外，已经发现，T1R2和T1R3受体的共表达导致对包括天然存在的和人造甜味剂的甜味刺激有反应的味觉受体。 本发明还涉及在测定中使用包含T1R1 / T1R3和T1R2 / T1R3的异低聚味觉感受器来鉴定分别响应于鲜味刺激和甜味刺激的化合物。 此外，本发明涉及稳定或瞬时共表达T1R1和T1R3的组合的细胞系的组成; 或T1R2和T1R3; 在组成型或诱导性条件下。 还提供了在基于细胞的测定中使用这些细胞系以鉴定鲜味和甜味调节化合物，特别是通过使用荧光成像检测受体活性的高通量筛选测定。 最后，本发明涉及一些发现，一些化合物，例如乳酸，抑制人T1R2 / T1R3和T1R1 / T1R3受体的活性，因此抑制甜味和鲜味，这表明这些受体可能是唯一的甜味和鲜味 受体。

    Methods of detecting T1R2- and T1R3-expressing cells potentially sensitive to sweet tastants
    7.
    发明授权
    Methods of detecting T1R2- and T1R3-expressing cells potentially sensitive to sweet tastants 有权
    检测对甜味滋味剂有潜在敏感性的T1R2-和T1R3表达细胞的方法

    公开(公告)号：US08168401B2

    公开(公告)日：2012-05-01

    申请号：US13023462

    申请日：2011-02-08

    Applicant: Mark Zoller ,  Xiaodong Li ,  Lena Staszewski ,  Shawn O'Connell ,  Sergey Zozulya ,  Jon Elliot Adler ,  Hong Xu ,  Femando Echeverri

    Inventor： Mark Zoller ,  Xiaodong Li ,  Lena Staszewski ,  Shawn O'Connell ,  Sergey Zozulya ,  Jon Elliot Adler ,  Hong Xu ,  Femando Echeverri

    IPC: G01N33/53 ,  G01N33/567

    CPC classification number: G01N33/5041 ,  A01K2217/05 ,  C07K14/705 ,  C07K2319/00 ,  C12Q1/6876 ,  C12Q2600/124 ,  C12Q2600/158 ,  G01N33/5008 ,  G01N33/566 ,  G01N2333/4719 ,  G01N2333/705 ,  G01N2333/726 ,  G01N2500/04

    Abstract: The present invention relates to the discovery that the T1R receptors assemble to form functional taste receptors. Particularly, it has been discovered that co-expression of T1R1 and T1R3 results in a taste receptor that responds to umami taste stimuli, including monosodium glutamate. Also, it has been discovered that co-expression of the T1R2 and T1R3 receptors results in a taste receptor that responds to sweet taste stimuli including naturally occurring and artificial sweeteners.Also the present invention relates to the use of hetero-oligomeric taste receptors comprising T1R1/T1R3 and T1R2/T1R3 in assays to identify compounds that respectively respond to umami taste stimuli and sweet taste stimuli.Further, the invention relates to the constitutive of cell lines that stably or transiently co-express a combination of T1R1 and T1R3; or T1R2 and T1R3; under constitutive or inducible conditions. The use of these cells lines in cell-based assays to identify umami and sweet taste modulatory compounds is also provided, particularly high throughput screening assays that detect receptor activity by use of fluorometric imaging.Finally, the invention relates to the discovery that some compounds, e.g., lactisole, inhibit both the activities of human T1R2/T1R3 and T1R1/T1R3 receptors, and accordingly the sweet and umami taste, suggesting that these receptors may be the only sweet and umami receptors.

    Abstract translation: 本发明涉及T1R受体组装形成功能性味觉受体的发现。 特别地，已经发现，T1R1和T1R3的共表达导致对鲜味刺激（包括谷氨酸钠）起反应的味觉感受器。 此外，已经发现，T1R2和T1R3受体的共表达导致对包括天然存在的和人造甜味剂的甜味刺激有反应的味觉受体。 本发明还涉及在测定中使用包含T1R1 / T1R3和T1R2 / T1R3的异低聚味觉感受器来鉴定分别响应于鲜味刺激和甜味刺激的化合物。 此外，本发明涉及稳定或瞬时共表达T1R1和T1R3的组合的细胞系的组成; 或T1R2和T1R3; 在组成型或诱导性条件下。 还提供了在基于细胞的测定中使用这些细胞系以鉴定鲜味和甜味调节化合物，特别是通过使用荧光成像检测受体活性的高通量筛选测定。 最后，本发明涉及一些发现，一些化合物，例如乳酸，抑制人T1R2 / T1R3和T1R1 / T1R3受体的活性，因此抑制甜味和鲜味，这表明这些受体可能是唯一的甜味和鲜味 受体。

    Heteromeric taste receptors comprising T1R2/T1R3 domains
    9.
    发明授权
    Heteromeric taste receptors comprising T1R2/T1R3 domains 有权
    含有T1R2 / T1R3结构域的异构味觉受体

    公开(公告)号：US08039590B2

    公开(公告)日：2011-10-18

    申请号：US12270857

    申请日：2008-11-14

    Applicant: Mark Zoller ,  Xiaodong Li ,  Lena Staszewski ,  Shawn O'Connell ,  Sergey Zozulya ,  Jon Elliot Adler ,  Hong Xu ,  Fernando Echeverri

    Inventor： Mark Zoller ,  Xiaodong Li ,  Lena Staszewski ,  Shawn O'Connell ,  Sergey Zozulya ,  Jon Elliot Adler ,  Hong Xu ,  Fernando Echeverri

    IPC: C07K1/00 ,  C07K14/00 ,  C07K17/00

    CPC classification number: G01N33/5008 ,  A01K2217/05 ,  C07K14/705 ,  G01N33/5041 ,  G01N33/566 ,  G01N2333/726

    Abstract: Heteromeric taste receptors are provided. These receptors comprise a first polypeptide which comprises extracellular and transmembrane domains wherein the transmembrane domains are at least 95% identical to the transmembrane domains of specific T1R2 polypeptides, and the extracellular domains are at least 95% identical to the corresponding extracellular domains of the specific T1R2 polypeptide or a different GPCR; and a second polypeptide which comprises extracellular and transmembrane domains wherein the transmembrane domains are at least 95% identical to the transmembrane domains of specific T1R3 polypeptides, and the extracellular domains are at least 95% identical to the corresponding extracellular domains of the specific T1R3 polypeptide or that of a different GPCR.

    Abstract translation: 提供异味味觉受体。 这些受体包含第一多肽，其包含细胞外和跨膜结构域，其中跨膜结构域与特异性T1R2多肽的跨膜结构域至少95％相同，并且细胞外结构域与特异性T1R2的相应胞外结构域至少95％相同 多肽或不同的GPCR; 和包含细胞外和跨膜结构域的第二多肽，其中所述跨膜结构域与特异性T1R3多肽的跨膜结构域至少95％相同，并且细胞外结构域与特异性T1R3多肽的相应细胞外结构域至少95％相同，或 不同的GPCR。

    T1R HETERO-OLIGOMERIC TASTE RECEPTORS
    10.
    发明申请
    T1R HETERO-OLIGOMERIC TASTE RECEPTORS 有权
    T1R异位低血糖受体

    公开(公告)号：US20110201046A1

    公开(公告)日：2011-08-18

    申请号：US13016887

    申请日：2011-01-28

    Applicant: Jon Elliot Adler ,  Xiaodong Li ,  Lena Staszewski ,  Hong Xu ,  Fernando Echeverri

    Inventor： Jon Elliot Adler ,  Xiaodong Li ,  Lena Staszewski ,  Hong Xu ,  Fernando Echeverri

    IPC: C12Q1/02 ,  C07K14/705

    CPC classification number: G01N33/5041 ,  C07K14/723 ,  G01N33/5008 ,  G01N33/5023 ,  G01N33/74 ,  G01N2333/705 ,  G01N2333/726 ,  G01N2500/00

    Abstract: Newly identified mammalian taste-cell-specific G protein-coupled receptors which function as hetero-oligomeric complexes in the sweet taste transduction pathway, and the genes and cDNA encoding said receptors arc described. Specifically, T1R G protein-coupled receptors active in sweet taste signaling as hetero-oligomeric complexes, and the genes and cDNA encoding the same, are described, along with methods for isolating such genes and for isolating and expressing such receptors. Methods for identifying putative taste modulating compounds using such hetero-oligomeric complexes also described, as is a novel surface expression facilitating peptide useful for targeting integral plasma membrane proteins to the surface of a cell.

    Abstract translation: 描述了新鉴定的哺乳动物味细胞特异性G蛋白偶联受体，其在甜味转导途径中用作异寡聚复合物，并描述了编码所述受体的基因和cDNA。 具体来说，描述了作为异寡聚复合物的甜味信号传导中起作用的T1R G蛋白偶联受体，以及编码相同基因和cDNA的方法，以及用于分离这些基因并分离和表达此类受体的方法。 还描述了使用这种异寡​​聚复合物鉴定推定的味觉调节化合物的方法，以及用于靶向细胞表面的整合质膜蛋白的新颖的表达表达促进肽。

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