公开(公告)号：US11542633B2

公开(公告)日：2023-01-03

申请号：US17728735

申请日：2022-04-25

Applicant: Inscripta, Inc.

Inventor： Richard Fox ,  Daniel Held

IPC: C40B50/06 ,  C12N9/22 ,  C40B30/04 ,  C40B40/10 ,  C07K16/28 ,  C07K14/74 ,  C40B70/00 ,  C12N15/81 ,  C12N15/85 ,  C12N15/90 ,  C40B20/04 ,  C40B40/02 ,  C12N15/10

Abstract: The present disclosure provides shuttle vectors for editing exogenous polynucleotides in heterologous live cells, as well as automated methods, modules, and multi-module cell editing instruments and systems for performing the editing methods.

公开(公告)号：US20220112508A1

公开(公告)日：2022-04-14

申请号：US17555395

申请日：2021-12-18

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Richard Fox ,  Phillip Belgrader ,  Don Masquelier

IPC: C12N15/68 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N15/11 ,  C12N9/22 ,  C12N15/90

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US20220112452A1

公开(公告)日：2022-04-14

申请号：US17555336

申请日：2021-12-17

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Richard Fox ,  Phillip Belgrader ,  Don Masquelier

IPC: C12M1/00 ,  C12M1/26 ,  C12N15/87 ,  C12M3/00

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US20210371799A1

公开(公告)日：2021-12-02

申请号：US17401362

申请日：2021-08-13

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Michael Graige ,  Richard Fox ,  Eileen Spindler ,  Amy Hiddessen ,  Phillip Belgrader ,  Don Masquelier ,  Bruce Chabansky

IPC: C12M1/42 ,  C12N15/11 ,  C12N15/90 ,  C12M3/00 ,  C12M1/26

Abstract: The present disclosure provides automated modules and instruments for improved detection of nuclease genome editing of live cells. The disclosure provides improved modules—including high throughput modules—for screening cells that have been subjected to editing and identifying and selecting cells that have been properly edited.

公开(公告)号：US20210317402A1

公开(公告)日：2021-10-14

申请号：US17355295

申请日：2021-06-23

Applicant: Inscripta, Inc.

Inventor： Eric Abbate ,  Katherine Krouse ,  Nandini Krishnamurthy ,  Richard Fox ,  Daniel Held ,  Michael Clay ,  Krishna Yerramsetty

IPC: C12N1/20 ,  C07K14/245

Abstract: The present disclosure relates to various different types of variants in E. coli coding and noncoding regions leading to enhanced lysine production for, e.g., supplements and nutraceuticals.

公开(公告)号：US20210317400A1

公开(公告)日：2021-10-14

申请号：US17355901

申请日：2021-06-23

Applicant: Inscripta, Inc.

Inventor： Phillip Belgrader ,  Don Masquelier ,  Andrew Garst ,  Richard Fox

IPC: C12M1/00 ,  C12M1/26 ,  C12N15/87 ,  C12M3/00

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US11142740B2

公开(公告)日：2021-10-12

申请号：US16536049

申请日：2019-08-08

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Michael Graige ,  Richard Fox ,  Eileen Spindler ,  Amy Hiddessen ,  Phillip Belgrader ,  Don Masquelier ,  Bruce Chabansky

IPC: C12M3/00 ,  C12N15/00 ,  B01L3/00 ,  C12M1/42 ,  C12N15/11 ,  C12N15/90 ,  C12M1/26

Abstract: The present disclosure provides automated modules and instruments for improved detection of nuclease genome editing of live cells. The disclosure provides improved modules—including high throughput modules—for screening cells that have been subjected to editing and identifying and selecting cells that have been properly edited.

公开(公告)号：US10920189B2

公开(公告)日：2021-02-16

申请号：US16904827

申请日：2020-06-18

Applicant: Inscripta, Inc.

Inventor： Richard Fox ,  Daniel Held ,  Eric Abbate ,  Michael Clay ,  Katherine Krouse ,  Nandini Krishnamurthy ,  Krishna Yerramsetty

IPC: C12N1/20 ,  C07K14/245

Abstract: The present disclosure relates to various different types of variants in E. coli coding and noncoding regions leading to enhanced lysine production for, e.g., supplements and nutraceuticals.

公开(公告)号：US10858761B2

公开(公告)日：2020-12-08

申请号：US16798338

申请日：2020-02-22

Applicant: Inscripta, Inc.

Inventor： Richard Fox ,  Daniel Held

IPC: C12N15/81 ,  C40B50/06 ,  C12N9/22 ,  C40B30/04 ,  C40B40/10 ,  C07K16/28 ,  C07K14/74 ,  C40B70/00 ,  C12N15/85 ,  C12N15/90 ,  C40B20/04 ,  C40B40/02 ,  C12N15/10

Abstract: The present disclosure provides shuttle vectors for editing exogenous polynucleotides in heterologous live cells, as well as automated methods, modules, and multi-module cell editing instruments and systems for performing the editing methods.

公开(公告)号：US10801008B1

公开(公告)日：2020-10-13

申请号：US16930269

申请日：2020-07-15

Applicant: Inscripta, Inc.

Inventor： Phillip Belgrader ,  Don Masquelier ,  Andrew Garst ,  Richard Fox

IPC: C12M1/00 ,  C12M3/00 ,  C12M1/26 ,  C12N15/87 ,  B01D71/02 ,  B01D63/08 ,  B01D69/06

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.