公开(公告)号：US11591592B2

公开(公告)日：2023-02-28

申请号：US17239540

申请日：2021-04-23

Applicant: Inscripta, Inc.

Inventor： Phillip Belgrader ,  Nathan Bade ,  Christian Siltanen ,  Aamir Mir ,  Xi-Jun Chen ,  Janine Mok ,  Burak Dura ,  Bruce Chabansky ,  David Stumbo ,  Eric Smith ,  Jorge Bernate

IPC: C12N15/11 ,  C12N15/10 ,  C12M1/34 ,  C12M1/26 ,  C12N15/113 ,  C12N15/86 ,  C12M3/00 ,  C12M3/04 ,  C12M1/00 ,  C12N5/074 ,  C12N15/88 ,  C12N15/90 ,  B01L3/00 ,  B01L7/00 ,  C12M1/32 ,  C12M3/06 ,  C12N9/22

Abstract: This invention relates to compositions of matter, methods, modules and automated, end-to-end closed instruments for automated mammalian cell growth, reagent bundle creation and mammalian cell transfection followed by nucleic acid-guided nuclease editing in live mammalian cells. The disclosed compositions and method entail making “reagent bundles” comprising many (hundreds of thousands to millions) clonal copies of an editing cassette and delivering or co-localizing the reagent bundles with live mammalian cells such that the editing cassettes edit the cells and the edited cells continue to grow.

公开(公告)号：US20210332389A1

公开(公告)日：2021-10-28

申请号：US17239540

申请日：2021-04-23

Applicant: Inscripta, Inc.

Inventor： Phillip Belgrader ,  Nathan Bade ,  Christian Siltanen ,  Aamir Mir ,  Xi-Jun Chen ,  Janine Mok ,  Burak Dura ,  Bruce Chabansky ,  David Stumbo ,  Eric Smith ,  Jorge Bernate

IPC: C12N15/90 ,  C12N15/10 ,  C12N5/074 ,  C12M1/00 ,  C12M3/00 ,  C12N15/88 ,  C12M3/04 ,  C12N15/11 ,  C12N9/22

Abstract: This invention relates to compositions of matter, methods, modules and automated, end-to-end closed instruments for automated mammalian cell growth, reagent bundle creation and mammalian cell transfection followed by nucleic acid-guided nuclease editing in live mammalian cells.

公开(公告)号：US20230250420A1

公开(公告)日：2023-08-10

申请号：US18174397

申请日：2023-02-24

Applicant: Inscripta, Inc.

Inventor： Phillip Belgrader ,  Nathan Bade ,  Christian Siltanen ,  Aamir Mir ,  Xi-Jun Chen ,  Janine Mok ,  Burak Dura ,  Bruce Chabansky ,  David Stumbo ,  Eric Smith ,  Jorge Bernate

IPC: C12N15/10 ,  C12M1/34 ,  C12M1/26 ,  C12N15/113 ,  C12N15/86 ,  C12M3/00 ,  C12M3/04 ,  C12M1/00 ,  C12N5/074 ,  C12N15/11 ,  C12N15/88 ,  C12N15/90 ,  B01L3/00 ,  B01L7/00 ,  C12M1/32 ,  C12M3/06

CPC classification number: C12N15/1082 ,  C12M41/36 ,  C12M33/14 ,  C12N15/113 ,  C12N15/86 ,  C12M23/42 ,  C12M27/10 ,  C12M29/04 ,  C12M43/00 ,  C12M47/02 ,  C12N5/0696 ,  C12N15/1065 ,  C12N15/1068 ,  C12N15/11 ,  C12N15/88 ,  C12N15/907 ,  B01L3/502761 ,  B01L7/00 ,  C12M23/12 ,  C12M23/16 ,  C12M23/50 ,  C12M47/04 ,  C12N9/22

Abstract: Compositions of matter, methods, modules, and automated instruments may relate to synthesizing a library including an editing cassette including a different gRNA and donor DNA pair, amplifying the editing cassette in a partition separate from other editing cassettes in the library, adding nuclease to the partition, and adding lipofectamine to the editing cassette and nuclease to form a lipofectamine/nucleic acid/nuclease complex. A microcarrier coated in extracellular matrix or a cell adhesion molecule coating may be added to the lipofectamine/nucleic acid/nuclease complex. Cell growth material, the microcarrier, and mammalian cells may be transferred to a growth module in an automated closed cell editing instrument via a liquid handling system. The mammalian cells may be allowed to seed on the microcarrier. Conditions may be provided for the mammalian cells to take-up and be edited by a payload associated with the lipofectamine/nucleic acid/nuclease complex. The mammalian cells may be detached from the microcarrier.