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    Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use
    5.
    发明申请
    Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use 有权
    氨基多胺胺氧化酶多核苷酸及相关多肽及其使用方法

    公开(公告)号：US20060162009A1

    公开(公告)日：2006-07-20

    申请号：US10743891

    申请日：2003-12-22

    申请人： Jonathan Duvick ,  Jacob Gilliam ,  Joyce Maddox ,  Aragula Rao ,  Oswald Crasta ,  Otto Folkerts

    发明人： Jonathan Duvick ,  Jacob Gilliam ,  Joyce Maddox ,  Aragula Rao ,  Oswald Crasta ,  Otto Folkerts

    IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N9/06 ,  C12N15/82 ,  C12N5/04

    CPC分类号： C12N9/0022 ,  C12N15/8242 ,  C12N15/8282

    摘要： The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera and Rhinocladiella atrovirens. The polynucleotides may be mutated to remove glycosylation sites and cysteine residues. Additionally, the present invention provides recombinant expression cassettes, host cells, transgenic plants, and transgenic seed. The present invention also provides for polynucleotides containing both APAO and a fumonisin esterase. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and eukaryotic systems, methods for detecting fumonisins, and methods for identifying transformed plant cells. Methods for degrading fungal toxins in plants, grain, grain processing, silage, food crops and in animal feed are also disclosed.

    摘要翻译： 本发明提供了从Exophiala spinifera和Rhinocladiella atvirens分离的酶APAO的多核苷酸和相关多肽。 可以突变多核苷酸以除去糖基化位点和半胱氨酸残基。 另外，本发明提供了重组表达盒，宿主细胞，转基因植物和转基因种子。 本发明还提供了含有APAO和伏马菌素酯酶的多核苷酸。 此外，本发明提供了在原核和真核系统中生产APAO酶的方法，用于检测伏马毒素的方法和用于鉴定转化的植物细胞的方法。 还公开了降解植物，谷物，谷物加工，青贮饲料，粮食作物和动物饲料中真菌毒素的方法。

    Hydroperoxide lyase gene from maize and methods of use
    7.
    发明授权
    Hydroperoxide lyase gene from maize and methods of use 有权
    来自玉米的过氧化氢裂解酶基因及其使用方法

    公开(公告)号：US06444874B1

    公开(公告)日：2002-09-03

    申请号：US09417704

    申请日：1999-10-13

    申请人： Jon Duvick ,  Jacob Gilliam

    发明人： Jon Duvick ,  Jacob Gilliam

    IPC分类号： A01H500

    CPC分类号： C12N15/8243 ,  C12N9/88 ,  C12N15/8279 ,  C12P7/04 ,  C12P7/24 ,  C12P7/26

    摘要： Compositions and methods for enhancing disease resistance, and modulating levels of flavor molecules in plants are provided. The compositions comprise nucleotide sequences encoding maize HPL genes. The methods comprise expressing hydroperoxide lyase (HPL) genes in plants, plant cells, and plant tissues. Expression cassettes comprising the HPL sequences of the invention are provided, as well as transformed plant cells, plant tissues, and plants.

    摘要翻译： 提供了用于增强抗病性和调节植物中风味分子水平的组合物和方法。 组合物包含编码玉米HPL基因的核苷酸序列。 该方法包括在植物，植物细胞和植物组织中表达氢过氧化物裂解酶（HPL）基因。 提供了包含本发明的HPL序列的表达盒，以及转化的植物细胞，植物组织和植物。

    Antifungal polypeptides
    8.
    发明申请
    Antifungal polypeptides 有权
    抗真菌多肽

    公开(公告)号：US20070192899A1

    公开(公告)日：2007-08-16

    申请号：US11657710

    申请日：2007-01-24

    申请人： Daniel Altier ,  I.A. Ellanskaya ,  Jacob Gilliam ,  Jennie Hunter-Cevera ,  James Presnail ,  Eric Schepers ,  Carl Simmons ,  Tamas Torok ,  Nasser Yalpani

    发明人： Daniel Altier ,  I.A. Ellanskaya ,  Jacob Gilliam ,  Jennie Hunter-Cevera ,  James Presnail ,  Eric Schepers ,  Carl Simmons ,  Tamas Torok ,  Nasser Yalpani

    IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N5/04 ,  C07K14/415 ,  A01N63/00

    CPC分类号： C12N15/8282 ,  A01N37/46 ,  A01N63/04 ,  C07K14/37

    摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include an amino acid sequence, and variants and fragments thereof, for an antipathogenic polypeptide that was isolated from a fungal fermentation broth. Nucleic acid molecules that encode the antipathogenic polypeptides of the invention, and antipathogenic domains thereof, are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention are also disclosed.

    摘要翻译： 提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。 组合物包括从真菌发酵液中分离的抗病原体多肽的氨基酸序列及其变体和片段。 还提供了编码本发明的抗病原体多肽的核酸分子及其抗病原结构域。 还提供使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。 该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。 还提供包含抗病原体多肽或包含本发明的核酸与载体组合的转化微生物的组合物，以及使用这些组合物保护植物免受病原体的方法。 还公开了包含编码本发明的抗病原体多肽的核苷酸序列的转化植物，植物细胞，种子和微生物。