公开(公告)号：US20170081712A1

公开(公告)日：2017-03-23

申请号：US15273200

申请日：2016-09-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Shifeng LI ,  Shoulian DONG ,  Theodore E. STRAUB ,  David N. KEYS

IPC: C12Q1/68 ,  G01N27/30 ,  G01N27/414

CPC classification number: C12Q1/6825 ,  C12Q1/6837 ,  C12Q1/6851 ,  G01N27/301 ,  G01N27/4145 ,  C12Q2531/113 ,  C12Q2563/116 ,  C12Q2563/159 ,  C12Q2565/607

Abstract: A system for detecting one or more target molecules includes a reaction device and a temperature controller. The reaction device comprises a plurality of reaction sites and plurality of field effect transistors corresponding to the plurality of reaction sites. At least two of the reaction sites may receive a reaction solution including two target molecules. The temperature controller is configured to control a temperature of at least one of the reaction sites or the reaction solution received by the at least one reaction site. At least some of the reaction sites may receive a reagent supporting an amplification reaction. When the reaction is subjected to an amplification assay, an amplified product is produced in the at least some of the reaction sites. The plurality of field effect transistors are configured to detect at least one of the amplified products.

公开(公告)号：US20170088879A1

公开(公告)日：2017-03-30

申请号：US15280160

申请日：2016-09-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David N. KEYS ,  Nivedita Sumi MAJUMDAR ,  Theodore E. STRAUB

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  B01L7/52 ,  B01L2300/024 ,  B01L2300/0829 ,  C12Q1/6851 ,  C12Q2527/107 ,  C12Q2545/114 ,  C12Q2561/113 ,  C12Q2563/159

Abstract: Systems and methods are described for quantifying a target nucleic acid. A sample comprising a target nucleic acid is segregated into a first plurality of the reaction volumes containing at least one target nucleic acid molecule and a second plurality of the reaction volumes containing no target nucleic acid molecules. The reaction volumes are subjected to an amplification assay, wherein the amplification assay is configured to amplify the target nucleic acid. An indicator of amplification is detected or measured in at least some of the plurality of reaction volumes. The target nucleic acid is quantified based on the detection or measurement. After discontinuing the amplification assay, the plurality of reaction volumes may be heated and changes in the indicators of amplification of two or more of the at least some of the reaction volumes may be detected or measured.