-
公开(公告)号：US08394933B2
公开(公告)日：2013-03-12
申请号：US12593713
申请日：2008-04-04
申请人： Masayuki Kawakami , Tatsuo Tsunoda , Hideaki Togashi , Takayuki Nara , Shun-ichi Matsuura , Chisato Sekikawa , Akiko Kawai , Akiyoshi Kawata , Fujio Mizukami , Kengo Sakaguchi
发明人： Masayuki Kawakami , Tatsuo Tsunoda , Hideaki Togashi , Takayuki Nara , Shun-ichi Matsuura , Chisato Sekikawa , Akiko Kawai , Akiyoshi Kawata , Fujio Mizukami , Kengo Sakaguchi
IPC分类号： C07K1/16 , G01N33/538 , A61K38/00
CPC分类号： G01N30/52 , B01D15/20 , B01D15/206 , B01D15/3804 , B01J20/183 , B01J20/2803 , B01J2220/58 , C07K1/113 , C07K1/18 , G01N2030/525 , Y10T428/2982
摘要： It is an object of the present invention to provide: a protein refolding column filler, which is effective for the refolding, namely, the activation of the function, of an inactive protein with an as yet unformed higher order structure produced in Escherichia coli or the like, or a protein whose conformation has been changed due to a certain cause and which has become inactivated; and a column filled with the aforementioned column filler. The present invention provides a protein refolding column filler, which comprises zeolite with BEA structure (Zeolite Beta) that is granulated into a particle state.
摘要翻译： 本发明的目的是提供：一种蛋白质重折叠柱填充剂，其对于在大肠杆菌中产生的尚未形成的高级结构的无活性蛋白质重新折叠，即功能的活化是有效的， 或由于某些原因而变形失活的蛋白质， 和填充有上述柱填料的柱。 本发明提供一种蛋白质重折叠柱填充剂，其包含粒状状态的具有BEA结构的沸石（沸石β）。
-
公开(公告)号：US20100210820A1
公开(公告)日：2010-08-19
申请号：US12593713
申请日：2008-04-04
申请人： Masayuki Kawakami , Tatsuo Tsunoda , Hideaki Togashi , Takayuki Nara , Shun-ichi Matsuura , Chisato Sekikawa , Akiko Kawai , Akiyoshi Kawata , Fujio Mizukami , Kengo Sakaguchi
发明人： Masayuki Kawakami , Tatsuo Tsunoda , Hideaki Togashi , Takayuki Nara , Shun-ichi Matsuura , Chisato Sekikawa , Akiko Kawai , Akiyoshi Kawata , Fujio Mizukami , Kengo Sakaguchi
CPC分类号： G01N30/52 , B01D15/20 , B01D15/206 , B01D15/3804 , B01J20/183 , B01J20/2803 , B01J2220/58 , C07K1/113 , C07K1/18 , G01N2030/525 , Y10T428/2982
摘要： It is an object of the present invention to provide: a protein refolding column filler, which is effective for the refolding, namely, the activation of the function, of an inactive protein with an as yet unformed higher order structure produced in Escherichia coli or the like, or a protein whose conformation has been changed due to a certain cause and which has become inactivated; and a column filled with the aforementioned column filler. The present invention provides a protein refolding column filler, which comprises zeolite with BEA structure (Zeolite Beta) that is granulated into a particle state.
摘要翻译： 本发明的目的是提供：一种蛋白质重折叠柱填充剂，其对于在大肠杆菌中产生的尚未形成的高级结构的无活性蛋白质重新折叠，即功能的活化是有效的， 或由于某些原因而变形失活的蛋白质， 和填充有上述柱填料的柱。 本发明提供一种蛋白质重折叠柱填充剂，其包含粒状状态的具有BEA结构的沸石（沸石β）。
-
公开(公告)号：US20060194279A1
公开(公告)日：2006-08-31
申请号：US10563870
申请日：2004-07-07
申请人： Fujio Mizukami , Yoshimichi Kiyozumi , Takuji Ikeda , Akiko Kawai , Takako Nagase , Kengo Sakaguchi , Hiroyuki Chiku
发明人： Fujio Mizukami , Yoshimichi Kiyozumi , Takuji Ikeda , Akiko Kawai , Takako Nagase , Kengo Sakaguchi , Hiroyuki Chiku
IPC分类号： C12P21/06 , C07K14/47 , C07K14/195 , C12N9/00
CPC分类号： C07K1/1133 , C07K1/1136 , C07K14/245
摘要： The present invention provides a method for refolding protein produced, for example, by Escherichia coli, that is inactive due to an as yet unformed higher order structure, or protein deactivated due to a change in conformation for some reason. The invention comprises a method, refolding kit, refolding agent, and molding that activate a native function or activity inherent to a protein through treatment with zeolite beta of protein produced, for example, by Escherichia coli, that is inactive due to an as yet unformed higher order structure, or protein deactivated due to a change in conformation for some reason. The invention also comprises a method for producing an active protein that utilizes the same. As compared with conventional methods, the present invention can provide a novel method for activating protein function that is highly versatile and generalizable, that employs a simple and easy protocol, and that is inexpensive and enables repeated use of the function activator.
摘要翻译： 本发明提供了一种重新折叠由例如大肠杆菌产生的蛋白质的方法，其由于尚未形成的高级结构而不活跃，或由于某种原因由于构象变化而失活的蛋白质。 本发明包括一种方法，重折叠试剂盒，重折叠剂和模制品，其通过用例如由大肠杆菌产生的蛋白质的β沸石处理而激活蛋白质固有的天然功能或活性，其由于尚未形成 由于某些原因由于构象变化而导致高阶结构或蛋白质失活。 本发明还包括一种制备利用其的活性蛋白质的方法。 与常规方法相比，本发明可以提供一种新颖的激活蛋白质功能的方法，该蛋白质功能具有高度通用性和可广泛性，采用简单易用的方案，价格便宜，能重复使用功能活化剂。
-
公开(公告)号：US20090005543A1
公开(公告)日：2009-01-01
申请号：US12204496
申请日：2008-09-04
申请人： Fujio MIZUKAMI , Yoshimichi Kiyozumi , Takuji Ikeda , Akiko Kawai , Takako Nagase , Kengo Sakaguchi , Hiroyuki Chiku
发明人： Fujio MIZUKAMI , Yoshimichi Kiyozumi , Takuji Ikeda , Akiko Kawai , Takako Nagase , Kengo Sakaguchi , Hiroyuki Chiku
CPC分类号： C07K1/1133 , C07K1/1136 , C07K14/245
摘要： The present invention provides a method for refolding protein produced, for example, by Escherichia coli, that is inactive due to an as yet unformed higher order structure, or protein deactivated due to a change in conformation for some reason. The invention comprises a method, refolding kit, refolding agent, and molding that activate a native function or activity inherent to a protein through treatment with zeolite beta of protein produced, for example, by Escherichia coli, that is inactive due to an as yet unformed higher order structure, or protein deactivated due to a change in conformation for some reason. The invention also comprises a method for producing an active protein that utilizes the same. As compared with conventional methods, the present invention can provide a novel method for activating protein function that is highly versatile and generalizable, that employs a simple and easy protocol, and that is inexpensive and enables repeated use of the function activator.
摘要翻译： 本发明提供了一种重新折叠由例如大肠杆菌产生的蛋白质的方法，其由于尚未形成的高级结构而不活跃，或由于某种原因由于构象变化而失活的蛋白质。 本发明包括一种方法，重折叠试剂盒，重折叠剂和模制品，其通过用例如由大肠杆菌产生的蛋白质的β沸石处理而激活蛋白质固有的天然功能或活性，其由于尚未形成 由于某些原因由于构象变化而导致高阶结构或蛋白质失活。 本发明还包括一种制备利用其的活性蛋白质的方法。 与常规方法相比，本发明可以提供一种新颖的激活蛋白质功能的方法，该蛋白质功能具有高度通用性和可广泛性，采用简单易用的方案，价格便宜，能重复使用功能活化剂。
-
-
-
搜索结果
4 条记录 (耗时 0.033 秒)
