公开(公告)号：US20090005543A1

公开(公告)日：2009-01-01

申请号：US12204496

申请日：2008-09-04

申请人： Fujio MIZUKAMI ,  Yoshimichi Kiyozumi ,  Takuji Ikeda ,  Akiko Kawai ,  Takako Nagase ,  Kengo Sakaguchi ,  Hiroyuki Chiku

发明人： Fujio MIZUKAMI ,  Yoshimichi Kiyozumi ,  Takuji Ikeda ,  Akiko Kawai ,  Takako Nagase ,  Kengo Sakaguchi ,  Hiroyuki Chiku

IPC分类号： C07K1/08 ,  C01B39/00 ,  B01J29/06

CPC分类号： C07K1/1133 ,  C07K1/1136 ,  C07K14/245

摘要： The present invention provides a method for refolding protein produced, for example, by Escherichia coli, that is inactive due to an as yet unformed higher order structure, or protein deactivated due to a change in conformation for some reason. The invention comprises a method, refolding kit, refolding agent, and molding that activate a native function or activity inherent to a protein through treatment with zeolite beta of protein produced, for example, by Escherichia coli, that is inactive due to an as yet unformed higher order structure, or protein deactivated due to a change in conformation for some reason. The invention also comprises a method for producing an active protein that utilizes the same. As compared with conventional methods, the present invention can provide a novel method for activating protein function that is highly versatile and generalizable, that employs a simple and easy protocol, and that is inexpensive and enables repeated use of the function activator.

摘要翻译： 本发明提供了一种重新折叠由例如大肠杆菌产生的蛋白质的方法，其由于尚未形成的高级结构而不活跃，或由于某种原因由于构象变化而失活的蛋白质。 本发明包括一种方法，重折叠试剂盒，重折叠剂和模制品，其通过用例如由大肠杆菌产生的蛋白质的β沸石处理而激活蛋白质固有的天然功能或活性，其由于尚未形成 由于某些原因由于构象变化而导致高阶结构或蛋白质失活。 本发明还包括一种制备利用其的活性蛋白质的方法。 与常规方法相比，本发明可以提供一种新颖的激活蛋白质功能的方法，该蛋白质功能具有高度通用性和可广泛性，采用简单易用的方案，价格便宜，能重复使用功能活化剂。

公开(公告)号：US20060194279A1

公开(公告)日：2006-08-31

申请号：US10563870

申请日：2004-07-07

申请人： Fujio Mizukami ,  Yoshimichi Kiyozumi ,  Takuji Ikeda ,  Akiko Kawai ,  Takako Nagase ,  Kengo Sakaguchi ,  Hiroyuki Chiku

发明人： Fujio Mizukami ,  Yoshimichi Kiyozumi ,  Takuji Ikeda ,  Akiko Kawai ,  Takako Nagase ,  Kengo Sakaguchi ,  Hiroyuki Chiku

IPC分类号： C12P21/06 ,  C07K14/47 ,  C07K14/195 ,  C12N9/00

CPC分类号： C07K1/1133 ,  C07K1/1136 ,  C07K14/245

摘要： The present invention provides a method for refolding protein produced, for example, by Escherichia coli, that is inactive due to an as yet unformed higher order structure, or protein deactivated due to a change in conformation for some reason. The invention comprises a method, refolding kit, refolding agent, and molding that activate a native function or activity inherent to a protein through treatment with zeolite beta of protein produced, for example, by Escherichia coli, that is inactive due to an as yet unformed higher order structure, or protein deactivated due to a change in conformation for some reason. The invention also comprises a method for producing an active protein that utilizes the same. As compared with conventional methods, the present invention can provide a novel method for activating protein function that is highly versatile and generalizable, that employs a simple and easy protocol, and that is inexpensive and enables repeated use of the function activator.

摘要翻译： 本发明提供了一种重新折叠由例如大肠杆菌产生的蛋白质的方法，其由于尚未形成的高级结构而不活跃，或由于某种原因由于构象变化而失活的蛋白质。 本发明包括一种方法，重折叠试剂盒，重折叠剂和模制品，其通过用例如由大肠杆菌产生的蛋白质的β沸石处理而激活蛋白质固有的天然功能或活性，其由于尚未形成 由于某些原因由于构象变化而导致高阶结构或蛋白质失活。 本发明还包括一种制备利用其的活性蛋白质的方法。 与常规方法相比，本发明可以提供一种新颖的激活蛋白质功能的方法，该蛋白质功能具有高度通用性和可广泛性，采用简单易用的方案，价格便宜，能重复使用功能活化剂。

公开(公告)号：US08663910B2

公开(公告)日：2014-03-04

申请号：US12562885

申请日：2009-09-18

申请人： Mikinaga Mori ,  Junichi Katada ,  Hiroyuki Chiku ,  Takayoshi Oyamada

发明人： Mikinaga Mori ,  Junichi Katada ,  Hiroyuki Chiku ,  Takayoshi Oyamada

IPC分类号： C12Q1/00

CPC分类号： G01N33/54366

摘要： A plurality of kinds of liquids, which are of at least three kinds, containing (a) a test body solution containing at least one kind of an analyte, and (b) at least two kinds of liquids selected from the group consisting of a reagent solution, an amplifying solution, and a detecting solution, are fed to a detection site containing a specific binding substance with respect to the analyte. A qualitative analysis or a quantitative analysis of the analyte contained in the test body solution is thereby performed. Directions of liquid feeding of all of the plurality of the kinds of the liquids vary from one another, and the plurality of the kinds of the liquids are caused to intersect with one another at the detection site.

摘要翻译： 至少三种的多种液体，其含有（a）含有至少一种分析物的测试体溶液，和（b）至少两种选自试剂 溶液，放大溶液和检测溶液相对于分析物进料到含有特异性结合物质的检测部位。 因此，进行了测试体溶液中包含的分析物的定性分析或定量分析。 所有多种液体的液体供给方向彼此不同，并且使多种液体在检测部位彼此相交。

公开(公告)号：US20100075440A1

公开(公告)日：2010-03-25

申请号：US12562885

申请日：2009-09-18

申请人： Mikinaga MORI ,  Jinichi Katada ,  Hiroyuki Chiku ,  Takayoshi Oyamada

发明人： Mikinaga MORI ,  Jinichi Katada ,  Hiroyuki Chiku ,  Takayoshi Oyamada

IPC分类号： G01N33/543

CPC分类号： G01N33/54366

摘要： A plurality of kinds of liquids, which are of at least three kinds, containing (a) a test body solution containing at least one kind of an analyte, and (b) at least two kinds of liquids selected from the group consisting of a reagent solution, an amplifying solution, and a detecting solution, are fed to a detection site containing a specific binding substance with respect to the analyte. A qualitative analysis or a quantitative analysis of the analyte contained in the test body solution is thereby performed. Directions of liquid feeding of all of the plurality of the kinds of the liquids vary from one another, and the plurality of the kinds of the liquids are caused to intersect with one another at the detection site.

摘要翻译： 至少三种的多种液体，其含有（a）含有至少一种分析物的测试体溶液，和（b）至少两种选自试剂 溶液，放大溶液和检测溶液相对于分析物进料到含有特异性结合物质的检测部位。 因此，进行了测试体溶液中包含的分析物的定性分析或定量分析。 所有多种液体的液体供给方向彼此不同，并且使多种液体在检测部位彼此相交。

公开(公告)号：US20060034026A1

公开(公告)日：2006-02-16

申请号：US11002193

申请日：2004-12-03

申请人： Hiroyuki Chiku ,  Shinji Kajihara ,  Yutaka Matsukuma ,  Kazuo Ueno ,  Akihiro Hamaguchi ,  Akira Kikuchi

发明人： Hiroyuki Chiku ,  Shinji Kajihara ,  Yutaka Matsukuma ,  Kazuo Ueno ,  Akihiro Hamaguchi ,  Akira Kikuchi

IPC分类号： H02H3/26

CPC分类号： H05K7/1449

摘要： An electronic device is composed of a reflector provided so as to cover a plug of a cabinet (sub-rack) into which a plug-in unit is plugged, and reflecting an electromagnetic wave which leaks from the plug; and a cable support supporting a cable connected to the plug-in unit and taken out of the plug, and forming an absorber which absorbs the electromagnetic wave reflected by the reflector.

摘要翻译： 电子设备由设置为覆盖插入单元插入的机柜（子架）的插头的反射器构成，并且反射从插头泄漏的电磁波; 以及电缆支撑件，其支撑连接到插入单元并从插头中取出的电缆，并且形成吸收由反射器反射的电磁波的吸收体。

公开(公告)号：US08716032B2

公开(公告)日：2014-05-06

申请号：US13523388

申请日：2012-06-14

申请人： Junichi Katada ,  Hiroyuki Chiku

发明人： Junichi Katada ,  Hiroyuki Chiku

IPC分类号： G01N33/553 ,  G01N33/558 ,  G01N33/563 ,  G01N33/543 ,  G01N33/53

CPC分类号： G01N33/54393 ,  G01N33/5308

摘要： The present invention is to provide an immunochromatography method which is capable of performing a detection with high-sensitivity or reduced false-positives by suppressing the occurrence of false-positive when a signal is amplified. An immunochromatography method includes in a state of where a complex of a test substance and a labeling substance containing a metal coupled with a first binding substance for the test substance is formed, developing the complex on an insoluble carrier in presence of a protease hydrolyzate of protein; capturing the test substance and the labeling substance at a detection site of the insoluble carrier containing a material which has a binding property to the first binding substance for the test substance or a second binding substance for the test substance; and detecting the test substance by amplifying the labeling substance captured.

摘要翻译： 本发明提供一种免疫色谱法，其能够通过抑制信号放大时的假阳性发生而通过高灵敏度或降低的假阳性进行检测。 免疫色谱方法包括在形成测试物质和含有与测试物质的第一结合物质偶联的金属的标记物质的复合物的状态下，在蛋白质蛋白酶水解物的存在下在不溶性载体上形成复合物 ; 在不溶性载体的检测部位捕获被检物质和标记物质，其含有与测试物质的第一结合物质或测试物质的第二结合物质具有结合性的物质; 并通过放大捕获的标记物质来检测被检物质。

公开(公告)号：US07998753B2

公开(公告)日：2011-08-16

申请号：US12325057

申请日：2008-11-28

申请人： Hiroyuki Chiku ,  Junichi Katada ,  Hideyuki Karaki ,  Hiroki Terada

发明人： Hiroyuki Chiku ,  Junichi Katada ,  Hideyuki Karaki ,  Hiroki Terada

IPC分类号： G01N33/53

CPC分类号： G01N33/558 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S435/96 ,  Y10S435/97 ,  Y10S436/808 ,  Y10S436/81

摘要： It is an object of the present invention to provide a measurement kit for developing a first developing solution and a second developing solution from different directions to suppress background noise, and an immunochromatography kit. The present invention provides a measurement kit, which comprises a first developing member for supplying a first developing solution and a second developing member for supplying a second developing solution, wherein the developing direction of the first developing solution is allowed to intersect with the developing direction of the second developing solution, so that development is carried out by developing the first and second developing solutions in different developing directions, and a water absorbent portion is established on the downstream of the developing directions.

摘要翻译： 本发明的目的是提供一种用于从不同方向开发第一显影液和第二显影液以抑制背景噪音的测量组件和免疫色谱仪。 本发明提供了一种测量套件，其包括用于供应第一显影液的第一显影构件和用于供应第二显影液的第二显影构件，其中使第一显影液的显影方向与显影方向相交， 第二显影液，通过在不同的显影方向显影第一和第二显影液进行显影，并且在显影方向的下游建立吸水部分。

公开(公告)号：US07935541B2

公开(公告)日：2011-05-03

申请号：US12239640

申请日：2008-09-26

申请人： Hiroyuki Chiku

发明人： Hiroyuki Chiku

IPC分类号： G01N33/553

CPC分类号： G01N33/558 ,  Y10S435/962 ,  Y10S436/826 ,  Y10T436/25375 ,  Y10T436/255

摘要： An object of the present invention is to overcome the problem of nonspecific adsorption of a labeled antibody on the detection site and to provide an immunochromatography method by which the false positive signal can be suppressed, and wherein the detection sensitivity is high and the highly reliable measurement can be performed. The present invention provides an immunochromatography method, which comprises developing an analyte and a labeling substance modified with a first antibody against the analyte on a porous carrier in a state where the analyte and the labeling substance is mixed, and capturing the analyte and the labeling substance at the reaction site on the porous carrier having a second antibody against the analyte, so as to detect the analyte; wherein a fragmented antibody is used as the first antibody and/or the second antibody; an analyte is detected via amplification using an amplification solution which contains a compound containing silver and a reducing agent for silver ions; and the ratio of the non-specifically adsorbed labeling substance in the pre-amplification detection site and the non-detection site of the porous carrier is between 0.4 to 2.5 in a system where the density of the non-specifically adsorbed labeling substance is 106/mm2 or less.

摘要翻译： 本发明的目的是克服在检测位点上非特异性吸附标记抗体的问题，提供可以抑制假阳性信号的免疫色谱法，其检测灵敏度高，可靠性高的测定 可以执行。 本发明提供一种免疫层析方法，其包括在分析物和标记物质混合的状态下，将分析物和用第一抗体修饰的标记物质发展到多孔载体上的分析物上，并捕获分析物和标记物质 在多孔载体上的反应位点具有针对分析物的第二抗体，以便检测分析物; 其中使用片段抗体作为第一抗体和/或第二抗体; 使用含有银的化合物和银离子还原剂的扩增溶液通过扩增检测分析物; 在非特异性吸附的标记物质的密度为106 / ml的体系中，非特异性吸附的标记物质在预扩增检测位点和多孔载体的非检测位点之间的比率为0.4〜2.5， mm2以下。

公开(公告)号：US08877515B2

公开(公告)日：2014-11-04

申请号：US13177971

申请日：2011-07-07

申请人： Hiroyuki Chiku ,  Junichi Katada ,  Hideyuki Karaki ,  Hiroki Terada

发明人： Hiroyuki Chiku ,  Junichi Katada ,  Hideyuki Karaki ,  Hiroki Terada

IPC分类号： G01N33/558

CPC分类号： G01N33/558 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S435/96 ,  Y10S435/97 ,  Y10S436/808 ,  Y10S436/81

摘要： It is an object of the present invention to provide a measurement kit for developing a first developing solution and a second developing solution from different directions to suppress background noise, and an immunochromatography kit. The present invention provides a measurement kit, which comprises a first developing member for supplying a first developing solution and a second developing member for supplying a second developing solution, wherein the developing direction of the first developing solution is allowed to intersect with the developing direction of the second developing solution, so that development is carried out by developing the first and second developing solutions in different developing directions, and a water absorbent portion is established on the downstream of each of the developing directions.

摘要翻译： 本发明的目的是提供一种用于从不同方向开发第一显影液和第二显影液以抑制背景噪音的测量组件和免疫色谱仪。 本发明提供了一种测量套件，其包括用于供应第一显影液的第一显影构件和用于供应第二显影液的第二显影构件，其中使第一显影液的显影方向与显影方向相交， 第二显影液，通过在不同的显影方向显影第一和第二显影液进行显影，并且在每个显影方向的下游形成吸水部分。

公开(公告)号：US08173444B2

公开(公告)日：2012-05-08

申请号：US12369433

申请日：2009-02-11

申请人： Bo Yang ,  Hiroyuki Chiku

发明人： Bo Yang ,  Hiroyuki Chiku

IPC分类号： G01N33/553

CPC分类号： G01N33/54386 ,  C08G65/3342 ,  C08L71/02 ,  C08L2203/02 ,  C09D171/02 ,  G01N33/532 ,  G01N33/585 ,  Y10T428/2982 ,  Y10T428/2991 ,  C08L2666/22

摘要： An object of the present invention is to provide a metal particle with which a highly-sensitive testing method can be conducted, and a testing method using such metal particle. The present invention provides a metal particle, which is produced by coating a metal particle labeled with a molecule binding to an analyte with a mixture of two or more types of water-soluble polymer having a mercapto group, a dithiol group or a sulfide group and differing in molecular weight.

摘要翻译： 本发明的目的是提供可以进行高灵敏度测试方法的金属颗粒和使用这种金属颗粒的测试方法。 本发明提供一种金属颗粒，其通过用分子结合分子标记的金属颗粒与两种或更多种具有巯基，二硫醇基或硫醚基的水溶性聚合物的混合物和 分子量不同。