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公开(公告)号：US06376248B1
公开(公告)日：2002-04-23
申请号：US09039780
申请日：1998-03-16
申请人： Pamela Hawley-Nelson , Jianqing Lan , PoJen Shih , Joel A. Jessee , Kevin P. Schifferli , Gulilat Gebeyehu , Valentina C. Ciccarone , Krista L. Evans
发明人： Pamela Hawley-Nelson , Jianqing Lan , PoJen Shih , Joel A. Jessee , Kevin P. Schifferli , Gulilat Gebeyehu , Valentina C. Ciccarone , Krista L. Evans
IPC分类号： C12N1588
CPC分类号： B82Y5/00 , A61K9/1272 , A61K47/54 , A61K47/543 , A61K47/645 , A61K47/6949 , A61K48/00 , A61K48/0025 , A61K48/0041 , C07K1/1077 , C07K7/06 , C07K7/08 , C07K14/005 , C12N15/87 , C12N2740/16322
摘要： The present invention provides compositions useful for transfecting eukaryotic cells comprising nucleic acid complexes with peptides, wherein the peptide is optionally covalently coupled to a nucleic acid-binding group, and cationic lipids or dendrimers as transfection agents. The invention also provides transfection compositions in which a peptide is covalently linked to the transfection agent (lipid, cationic lipid or dendrimer). Inclusion of peptides or modified-peptides in transfection compositions or covalent attachment of peptides to transfection agents results in enhanced transfection efficiency. Methods for the preparation of transfection compositions and methods of using these transfection compositions as intracellular delivery agents and extracellular targeting agents are also disclosed.
摘要翻译： 本发明提供了可用于转染包含与肽的核酸复合物的真核细胞的组合物，其中肽任选地与核酸结合基团共价连接，并且阳离子脂质或树状聚合物作为转染剂。 本发明还提供其中肽与转染剂（脂质，阳离子脂质或树枝状大分子）共价连接的转染组合物。 在转染组合物中包含肽或修饰肽或将肽共价连接到转染试剂导致增强的转染效率。 还公开了制备转染组合物的方法和使用这些转染组合物作为细胞内递送剂和细胞外靶向剂的方法。
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公开(公告)号：US08058068B2
公开(公告)日：2011-11-15
申请号：US10200879
申请日：2002-07-23
申请人： Pamela Hawley-Nelson , Jianqing Lan , PoJen Shih , Joel A. Jessee , Kevin P. Schifferli , Gulilat Gebeyehu , Valentina C. Ciccarone , Krista L. Evans
发明人： Pamela Hawley-Nelson , Jianqing Lan , PoJen Shih , Joel A. Jessee , Kevin P. Schifferli , Gulilat Gebeyehu , Valentina C. Ciccarone , Krista L. Evans
IPC分类号： C12N15/88 , C07K14/00 , C07K14/025 , C07H21/02 , C07H21/04
CPC分类号： B82Y5/00 , A61K9/1272 , A61K38/00 , A61K47/543 , A61K47/645 , A61K47/6911 , A61K48/00 , A61K48/0025 , A61K48/0041 , C07K1/1077 , C07K7/06 , C07K7/08 , C07K14/005 , C07K2319/09 , C12N15/87 , C12N15/88 , C12N2740/16322 , C12N2810/405 , C12N2810/60 , C12N2810/6018 , C12N2810/6054 , C12N2810/6081 , Y10T428/2984
摘要： The present invention provides compositions useful for transfecting eukaryotic cells comprising nucleic acid complexes with peptides, wherein the peptide is optionally covalently coupled to a nucleic acid-binding group, and cationic lipids or dendrimers as transfection agents. The invention also provides transfection compositions in which a peptide is covalently linked to the transfection agent (lipid, cationic lipid or dendrimer). Inclusion of peptides or modified-peptides in transfection compositions or covalent attachment of peptides to transfection agents results in enhanced transfection efficiency. Methods for the preparation of transfection compositions and methods of using these transfection compositions as intracellular delivery agents and extracellular targeting agents are also disclosed.
摘要翻译： 本发明提供了可用于转染包含与肽的核酸复合物的真核细胞的组合物，其中肽任选地与核酸结合基团共价连接，并且阳离子脂质或树状聚合物作为转染剂。 本发明还提供其中肽与转染剂（脂质，阳离子脂质或树枝状大分子）共价连接的转染组合物。 在转染组合物中包含肽或修饰肽或将肽共价连接到转染试剂导致增强的转染效率。 还公开了制备转染组合物的方法和使用这些转染组合物作为细胞内递送剂和细胞外靶向剂的方法。
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公开(公告)号：US6051429A
公开(公告)日：2000-04-18
申请号：US818200
申请日：1997-03-14
申请人： Pamela Hawley-Nelson , Jianqing Lan , PoJen Shih , Joel A. Jessee , Kevin P. Schifferli , Gulilat Gebeyehu
发明人： Pamela Hawley-Nelson , Jianqing Lan , PoJen Shih , Joel A. Jessee , Kevin P. Schifferli , Gulilat Gebeyehu
IPC分类号： C12N15/09 , A61K9/127 , A61K47/48 , A61K48/00 , A61K49/00 , C07K1/107 , C07K7/06 , C07K7/08 , C07K14/00 , C07K14/16 , C12N1/00 , C12N15/87 , C12P21/00 , C12N15/64 , C12N7/00 , C12N15/11 , C12N15/63
CPC分类号： B82Y5/00 , A61K47/48023 , A61K47/48046 , A61K47/48315 , A61K47/48961 , A61K48/00 , A61K48/0025 , A61K48/0041 , A61K9/1272 , C07K1/1077 , C07K14/005 , C07K7/06 , C07K7/08 , C12N15/87 , C12N2740/16322
摘要： The present invention provides compositions useful for transfecting eukaryotic cells comprising nucleic acid complexes with peptides, wherein the peptide is optionally covalently coupled to a nucleic acid-binding group, and cationic lipids or dendrimers as transfection agents. The invention also provides transfection compositions in which a peptide is covalently linked to the transfection agent (lipid, cationic lipid or dendrimer). Inclusion of peptides or modified-peptides in transfection compositions or covalent attachment of peptides to transfection agents results in enhanced transfection efficiency. Methods for the preparation of transfection compositions and methods of using these transfection compositions as intracellular delivery agents and extracellular targeting agents are also disclosed.
摘要翻译： 本发明提供了可用于转染包含与肽的核酸复合物的真核细胞的组合物，其中肽任选地与核酸结合基团共价连接，并且阳离子脂质或树状聚合物作为转染剂。 本发明还提供其中肽与转染剂（脂质，阳离子脂质或树枝状大分子）共价连接的转染组合物。 在转染组合物中包含肽或修饰肽或将肽共价连接到转染试剂导致增强的转染效率。 还公开了制备转染组合物的方法和使用这些转染组合物作为细胞内递送剂和细胞外靶向剂的方法。
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公开(公告)号：US5736392A
公开(公告)日：1998-04-07
申请号：US658130
申请日：1996-06-04
IPC分类号： C12N15/09 , A61K9/127 , A61K31/70 , A61K35/76 , A61K48/00 , C12N7/04 , C12N15/88 , C12N15/00 , C07K14/005 , G01N33/92
CPC分类号： A61K9/1272 , A61K47/48046 , C12N15/88
摘要： The present invention discloses compositions useful for transfecting eukaryotic cells comprising nucleic acid complexes with peptides, proteins or protein fragments, wherein the peptide is optionally covalently coupled to a DNA-binding group, and cationic lipids useful for transfecting eukaryotic cells. Methods for the preparation of transfecting compositions and use as intracellular delivery agents and extracellular targeting agents are also disclosed.
摘要翻译： 本发明公开了用于转染包含与肽，蛋白质或蛋白质片段的核酸复合物的真核细胞的组合物，其中肽任选地与DNA结合基团共价连接，以及可用于转染真核细胞的阳离子脂质。 还公开了用于制备转染组合物和用作细胞内递送剂和细胞外靶向剂的方法。
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公开(公告)号：US20060229246A1
公开(公告)日：2006-10-12
申请号：US11333982
申请日：2006-01-17
申请人： Pamela Hawley-Nelson , Jianqing Lan , PoJen Shih , Joel Jessee , Keivn Schifferli , Gulilat Gebeyehu , Valentina Ciccarone , Krista Evans
发明人： Pamela Hawley-Nelson , Jianqing Lan , PoJen Shih , Joel Jessee , Keivn Schifferli , Gulilat Gebeyehu , Valentina Ciccarone , Krista Evans
CPC分类号： B82Y5/00 , A61K9/1272 , A61K38/00 , A61K47/543 , A61K47/645 , A61K47/6911 , A61K48/00 , A61K48/0025 , A61K48/0041 , C07K1/1077 , C07K7/06 , C07K7/08 , C07K14/005 , C07K2319/09 , C12N15/87 , C12N15/88 , C12N2740/16322 , C12N2810/405 , C12N2810/60 , C12N2810/6018 , C12N2810/6054 , C12N2810/6081 , Y10T428/2984
摘要： The present invention provides compositions useful for transfecting eukaryotic cells comprising nucleic acid complexes with peptides, wherein the peptide is optionally covalently coupled to a nucleic acid-binding group, and cationic lipids or dendrimers as transfection agents. The invention also provides transfection compositions in which a peptide is covalently linked to the transfection agent (lipid, cationic lipid or dendrimer). Inclusion of peptides or modified-peptides in transfection compositions or covalent attachment of peptides to transfection agents results in enhanced transfection efficiency. Methods for the preparation of transfection compositions and methods of using these transfection compositions as intracellular delivery agents and extracellular targeting agents are also disclosed.
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公开(公告)号：US5627159A
公开(公告)日：1997-05-06
申请号：US329969
申请日：1994-10-27
申请人： PoJen Shih , Pamela Hawley-Nelson , Joel A. Jessee
发明人： PoJen Shih , Pamela Hawley-Nelson , Joel A. Jessee
CPC分类号： A61K9/1272 , C12N15/87
摘要： A method is provided for enhancing transfection efficiency of eukaryotic cells comprising contacting said cells with a lipid aggregate comprising nucleic acid and a polycationic lipid composition in the presence of a polycationic compound and serum. The polycationic compound is preferably Polybrene.TM., and the lipid aggregate preferably comprises liposomes of nucleic acid and LipofectAMINE.TM..
摘要翻译： 提供了一种用于增强真核细胞的转染效率的方法，包括在聚阳离子化合物和血清存在下使所述细胞与包含核酸和聚阳离子脂质组合物的脂质聚集体接触。 聚阳离子化合物优选为Polybrene TM，脂质聚集体优选包含核酸和LipofectAMINE TM的脂质体。
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