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    Method for producing, in yeast, a hydroxylated triple helical protein, and yeast host cells useful in said method
    1.
    发明授权
    Method for producing, in yeast, a hydroxylated triple helical protein, and yeast host cells useful in said method 失效
    在酵母中生产羟基化三螺旋蛋白的方法和用于所述方法的酵母宿主细胞

    公开(公告)号：US06451557B1

    公开(公告)日：2002-09-17

    申请号：US09297269

    申请日：1999-04-28

    申请人： Paul Richard Vaughan ,  Maria Galanis ,  John Alan Maurice Ramshaw ,  Jerome Anthony Werkmeister

    发明人： Paul Richard Vaughan ,  Maria Galanis ,  John Alan Maurice Ramshaw ,  Jerome Anthony Werkmeister

    IPC分类号： C12P2102

    CPC分类号： C12N9/0071 ,  A61K38/00 ,  C07K14/78 ,  C12P21/02

    摘要： A method for producing a hydroxylated triple helical protein is disclosed, as well as a yeast host cell used in the method. The method comprises culturing a yeast host cell comprising: (1) a first DNA molecule comprising a DNA sequence encoding P4H&agr; subunit operably linked to a promoter functional in the yeast host cell, (2) a second DNA molecule comprising a DNA sequence encoding P4H&agr; subunit operably linked to a promoter functional in the yeast host cell, and (3) a third DNA molecule comprising a DNA sequence encoding a polypeptide or peptide operably linked to a promoter functional in the yeast host cell, wherein the polypeptide or peptide is one which, when hydroxylated, forms the hydroxylated triple helical protein; and wherein during culturing, each of the first DNA molecule, the second DNA molecule and the third DNA molecule are replicated, stably retained and segregated.

    摘要翻译： 公开了一种生产羟基化三螺旋蛋白的方法，以及用于该方法的酵母宿主细胞。 该方法包括培养酵母宿主细胞，其包括：（1）第一DNA分子，其包含编码与在酵母宿主细胞中有功能的启动子可操作地连接的P4Halpha亚基的DNA序列，（2）第二DNA分子，其包含编码P4Halpha亚单位的DNA序列 连接到在酵母宿主细胞中起促进作用的启动子，和（3）第三DNA分子，其包含可操作地连接到在酵母宿主细胞中有功能的启动子的多肽或肽的DNA序列，其中多肽或肽是， 当羟基化时，形成羟基化三螺旋蛋白; 并且其中在培养过程中，复制，稳定地保留和分离第一DNA分子，第二DNA分子和第三DNA分子。