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    Method for producing, in yeast, a hydroxylated triple helical protein, and yeast host cells useful in said method
    2.
    发明授权
    Method for producing, in yeast, a hydroxylated triple helical protein, and yeast host cells useful in said method 失效
    在酵母中生产羟基化三螺旋蛋白的方法和用于所述方法的酵母宿主细胞

    公开(公告)号：US06451557B1

    公开(公告)日：2002-09-17

    申请号：US09297269

    申请日：1999-04-28

    申请人： Paul Richard Vaughan ,  Maria Galanis ,  John Alan Maurice Ramshaw ,  Jerome Anthony Werkmeister

    发明人： Paul Richard Vaughan ,  Maria Galanis ,  John Alan Maurice Ramshaw ,  Jerome Anthony Werkmeister

    IPC分类号： C12P2102

    CPC分类号： C12N9/0071 ,  A61K38/00 ,  C07K14/78 ,  C12P21/02

    摘要： A method for producing a hydroxylated triple helical protein is disclosed, as well as a yeast host cell used in the method. The method comprises culturing a yeast host cell comprising: (1) a first DNA molecule comprising a DNA sequence encoding P4H&agr; subunit operably linked to a promoter functional in the yeast host cell, (2) a second DNA molecule comprising a DNA sequence encoding P4H&agr; subunit operably linked to a promoter functional in the yeast host cell, and (3) a third DNA molecule comprising a DNA sequence encoding a polypeptide or peptide operably linked to a promoter functional in the yeast host cell, wherein the polypeptide or peptide is one which, when hydroxylated, forms the hydroxylated triple helical protein; and wherein during culturing, each of the first DNA molecule, the second DNA molecule and the third DNA molecule are replicated, stably retained and segregated.

    摘要翻译： 公开了一种生产羟基化三螺旋蛋白的方法，以及用于该方法的酵母宿主细胞。 该方法包括培养酵母宿主细胞，其包括：（1）第一DNA分子，其包含编码与在酵母宿主细胞中有功能的启动子可操作地连接的P4Halpha亚基的DNA序列，（2）第二DNA分子，其包含编码P4Halpha亚单位的DNA序列 连接到在酵母宿主细胞中起促进作用的启动子，和（3）第三DNA分子，其包含可操作地连接到在酵母宿主细胞中有功能的启动子的多肽或肽的DNA序列，其中多肽或肽是， 当羟基化时，形成羟基化三螺旋蛋白; 并且其中在培养过程中，复制，稳定地保留和分离第一DNA分子，第二DNA分子和第三DNA分子。

    Preparation and Use of Basement Membrane Particles
    5.
    发明申请
    Preparation and Use of Basement Membrane Particles 审中-公开
    基底膜颗粒的制备与使用

    公开(公告)号：US20090104593A1

    公开(公告)日：2009-04-23

    申请号：US11921038

    申请日：2006-05-29

    申请人： Jerome Werkmeister ,  John Alan Maurice Ramshaw ,  Veronica Glattauer

    发明人： Jerome Werkmeister ,  John Alan Maurice Ramshaw ,  Veronica Glattauer

    IPC分类号： A01N1/02 ,  C12N5/06

    CPC分类号： C12N5/0068 ,  C12N2533/90

    摘要： A method for supporting the in vitro growth of one or more eukaryotic cell type(s), the method comprising; seeding cells of said cell type(s) onto particles comprising basement membrane or a basement membrane-like substrate, and culturing said seeded cells in vitro under conditions suitable for expansion of said seeded cells, wherein said particles are less than about 500 μm in size.

    摘要翻译： 一种用于支持一种或多种真核细胞类型的体外生长的方法，所述方法包括： 将所述细胞类型的细胞接种到包含基底膜或基底膜样底物的颗粒上，并在适于扩展所述接种的细胞的条件下体外培养所述接种的细胞，其中所述颗粒的尺寸小于约500μm 。

    Methods and devices for tissue repair
    7.
    发明申请
    Methods and devices for tissue repair 审中-公开
    组织修复的方法和装置

    公开(公告)号：US20090098177A1

    公开(公告)日：2009-04-16

    申请号：US12292169

    申请日：2008-11-13

    申请人： Jerome Anthony Werkmeister ,  Wei-Bor Tsai ,  John Alan Maurice Ramshaw ,  Helmut Werner Thissen ,  Ken-Yuan Chang

    发明人： Jerome Anthony Werkmeister ,  Wei-Bor Tsai ,  John Alan Maurice Ramshaw ,  Helmut Werner Thissen ,  Ken-Yuan Chang

    IPC分类号： A61K9/00 ,  A61K35/12

    CPC分类号： A61L27/3886 ,  A61K35/12 ,  A61L27/3608 ,  A61L27/3804 ,  A61L27/3817 ,  A61L27/3852 ,  A61L27/3895 ,  A61L27/52 ,  A61L2400/06 ,  A61L2430/06 ,  C12N5/0075 ,  C12N5/0653 ,  C12N5/0654 ,  C12N5/0655 ,  C12N5/0656 ,  C12N2533/40 ,  C12N2533/54

    摘要： Methods for treating diseased or damaged tissue in a subject are disclosed, involving administering to said subject at a site wherein diseased or damaged tissue occurs, cells of a type(s) normally found in healthy tissue corresponding to the diseased or damaged tissue, and/or suitable progenitor cells thereof, in association with bioresorbable beads or particles and optionally a gel and/or gel-forming substance. Where the cells an/or suitable progenitor cells thereof are chondrocytes, embryonic stem cells and/or bone marrow stromal cells, the methods of the invention are suitable for treating, for example, articular cartilage degeneration associated with primary osteoarthritis. Also disclosed is a device having tissue-like characteristics for treating diseased or damaged tissue in a subject, wherein the device comprises cells of a type(s) normally found in healthy tissue corresponding to the diseased or damaged tissue, and/or suitable progenitor cells thereof, in association with bioresorbable beads or particles and optionally a gel and/or gel-forming substance.

    摘要翻译： 公开了用于治疗受试者中的患病或受损组织的方法，包括在发生病变或损伤的组织的部位施用所述受试者，通常在对应于患病或损伤的组织的健康组织中发现的细胞和/ 或其合适的祖细胞，其与生物可再吸收的珠粒或颗粒以及任选的凝胶和/或凝胶形成物质结合。 在细胞/或其合适的祖细胞是软骨细胞，胚胎干细胞和/或骨髓基质细胞的情况下，本发明的方法适用于治疗例如与原发性骨关节炎相关的关节软骨变性。 还公开了一种具有用于治疗受试者中的患病或受损组织的组织样特征的装置，其中该装置包括通常存在于对应于患病或受损组织的健康组织中的类型的细胞和/或合适的祖细胞 与生物可再吸收的珠粒或颗粒以及任选的凝胶和/或凝胶形成物质结合。