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公开(公告)号：US20130260432A1
公开(公告)日：2013-10-03
申请号：US13436612
申请日：2012-03-30
申请人： Richard R. BOTT , Marguerite A. Cervin , James T. Kellis, JR. , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Christopher Lee Rife , Derek H. Wells , Walter Weyler , Gregory M. Whited
发明人： Richard R. BOTT , Marguerite A. Cervin , James T. Kellis, JR. , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Christopher Lee Rife , Derek H. Wells , Walter Weyler , Gregory M. Whited
CPC分类号： C12N9/88 , C12P5/007 , C12Y402/03027
摘要： The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
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2.发明授权公开(公告)号：US08916370B2
公开(公告)日：2014-12-23
申请号：US13436612
申请日：2012-03-30
申请人： Richard R. Bott , Marguerite A. Cervin , James T. Kellis, Jr. , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Christopher Lee Rife , Derek H. Wells , Walter Weyler , Gregory M. Whited
发明人： Richard R. Bott , Marguerite A. Cervin , James T. Kellis, Jr. , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Christopher Lee Rife , Derek H. Wells , Walter Weyler , Gregory M. Whited
IPC分类号： C12N9/88 , C12P21/06 , C12N15/00 , C12P5/02 , C12N1/20 , C12N1/00 , C07H21/04 , C07K1/00 , C12P5/00
CPC分类号： C12N9/88 , C12P5/007 , C12Y402/03027
摘要： The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
摘要翻译： 本发明提供包含至少一种具有改善的催化活性和/或溶解度的异戊二烯合酶的方法和组合物。 特别地，本发明提供用于在微生物宿主细胞中增加异戊二烯生产的变体植物异戊二烯合酶。 本发明的生物合成生产的异戊二烯可用于制造橡胶和弹性体。
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3.发明授权公开(公告)号：US08173410B2
公开(公告)日：2012-05-08
申请号：US12429143
申请日：2009-04-23
申请人： Richard R. Bott , Marguerite A. Cervin , James T. Kellis, Jr. , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Christopher Lee Rife , Derek H. Wells , Walter Weyler , Gregory M. Whited
发明人： Richard R. Bott , Marguerite A. Cervin , James T. Kellis, Jr. , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Christopher Lee Rife , Derek H. Wells , Walter Weyler , Gregory M. Whited
CPC分类号： C12N9/88 , C12P5/007 , C12Y402/03027
摘要： The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
摘要翻译： 本发明提供包含至少一种具有改善的催化活性和/或溶解度的异戊二烯合酶的方法和组合物。 特别地，本发明提供用于在微生物宿主细胞中增加异戊二烯生产的变体植物异戊二烯合酶。 本发明的生物合成生产的异戊二烯可用于制造橡胶和弹性体。
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4.发明申请公开(公告)号：US20100003716A1
公开(公告)日：2010-01-07
申请号：US12429143
申请日：2009-04-23
申请人： Marguerite A. Cervin , Gregory M. Whited , Andrei Miasnikov , Caroline M. Peres , Walter Weyler , Derek H. Wells , Richard R. Bott
发明人： Marguerite A. Cervin , Gregory M. Whited , Andrei Miasnikov , Caroline M. Peres , Walter Weyler , Derek H. Wells , Richard R. Bott
CPC分类号： C12N9/88 , C12P5/007 , C12Y402/03027
摘要： The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
摘要翻译： 本发明提供包含至少一种具有改善的催化活性和/或溶解度的异戊二烯合酶的方法和组合物。 特别地，本发明提供用于在微生物宿主细胞中增加异戊二烯生产的变体植物异戊二烯合酶。 本发明的生物合成生产的异戊二烯可用于制造橡胶和弹性体。
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公开(公告)号：US08288148B2
公开(公告)日：2012-10-16
申请号：US12335071
申请日：2008-12-15
申请人： Marguerite A. Cervin , Gopal K. Chotani , Frank J. Feher , Richard La Duca , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Aaron S. Puhala , Karl J. Sanford , Fernando Valle , Gregory M. Whited
发明人： Marguerite A. Cervin , Gopal K. Chotani , Frank J. Feher , Richard La Duca , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Aaron S. Puhala , Karl J. Sanford , Fernando Valle , Gregory M. Whited
CPC分类号： C12P5/007 , C12N9/1022 , C12N9/1205 , C12N9/1229 , C12N9/88 , C12N9/90 , C12N15/52 , C12Y203/01009 , C12Y207/01036 , C12Y401/01033 , C12Y402/03027 , C12Y503/03002 , Y02E50/343
摘要： The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.
摘要翻译： 本发明的特征在于从培养细胞产生异戊二烯的方法。 本发明还提供包括这些培养细胞的组合物。
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公开(公告)号：US20130078699A1
公开(公告)日：2013-03-28
申请号：US13600132
申请日：2012-08-30
申请人： Marguerite A. CERVIN , Gopal K. Chotani , Frank J. Feher , Richard La Duca , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Aaron S. Puhala , Karl J. Sanford , Fernando Valle , Gregory M. Whited
发明人： Marguerite A. CERVIN , Gopal K. Chotani , Frank J. Feher , Richard La Duca , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Aaron S. Puhala , Karl J. Sanford , Fernando Valle , Gregory M. Whited
IPC分类号： C12P5/00
CPC分类号： C12P5/007 , C12N9/1022 , C12N9/1205 , C12N9/1229 , C12N9/88 , C12N9/90 , C12N15/52 , C12Y203/01009 , C12Y207/01036 , C12Y401/01033 , C12Y402/03027 , C12Y503/03002 , Y02E50/343
摘要： The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.
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公开(公告)号：US08709785B2
公开(公告)日：2014-04-29
申请号：US13600109
申请日：2012-08-30
申请人： Marguerite A. Cervin , Gopal K. Chotani , Frank J. Feher , Richard La Duca , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Aaron S. Puhala , Karl J. Sanford , Fernando Valle , Gregory M. Whited
发明人： Marguerite A. Cervin , Gopal K. Chotani , Frank J. Feher , Richard La Duca , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Aaron S. Puhala , Karl J. Sanford , Fernando Valle , Gregory M. Whited
CPC分类号： C12P5/007 , C12N9/1022 , C12N9/1205 , C12N9/1229 , C12N9/88 , C12N9/90 , C12N15/52 , C12Y203/01009 , C12Y207/01036 , C12Y401/01033 , C12Y402/03027 , C12Y503/03002 , Y02E50/343
摘要： The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.
摘要翻译： 本发明的特征在于从培养细胞产生异戊二烯的方法。 本发明还提供包括这些培养细胞的组合物。
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公开(公告)号：US09260727B2
公开(公告)日：2016-02-16
申请号：US13600132
申请日：2012-08-30
申请人： Marguerite A. Cervin , Gopal K. Chotani , Frank J. Feher , Richard La Duca , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Aaron S. Puhala , Karl J. Sanford , Fernando Valle , Gregory M. Whited
发明人： Marguerite A. Cervin , Gopal K. Chotani , Frank J. Feher , Richard La Duca , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Aaron S. Puhala , Karl J. Sanford , Fernando Valle , Gregory M. Whited
IPC分类号： C12N1/20 , C12P7/42 , C12P5/02 , C12N15/00 , C12N15/74 , C07H21/04 , C12P5/00 , C12N9/10 , C12N9/12 , C12N9/88 , C12N9/90
CPC分类号： C12P5/007 , C12N9/1022 , C12N9/1205 , C12N9/1229 , C12N9/88 , C12N9/90 , C12N15/52 , C12Y203/01009 , C12Y207/01036 , C12Y401/01033 , C12Y402/03027 , C12Y503/03002 , Y02E50/343
摘要： The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.
摘要翻译： 本发明的特征在于从培养细胞产生异戊二烯的方法。 本发明还提供包括这些培养细胞的组合物。
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公开(公告)号：US20130071908A1
公开(公告)日：2013-03-21
申请号：US13600109
申请日：2012-08-30
申请人： Marguerite A. CERVIN , Gregory M. Whited , Gopal K. Chotani , Fernando Valle , Karl J. Sanford , Joseph C. McAuliffe , Frank J. Feher , Aaron S. Puhala , Andrei Miasnikov , Richard La Duca , Caroline M. Peres
发明人： Marguerite A. CERVIN , Gregory M. Whited , Gopal K. Chotani , Fernando Valle , Karl J. Sanford , Joseph C. McAuliffe , Frank J. Feher , Aaron S. Puhala , Andrei Miasnikov , Richard La Duca , Caroline M. Peres
CPC分类号： C12P5/007 , C12N9/1022 , C12N9/1205 , C12N9/1229 , C12N9/88 , C12N9/90 , C12N15/52 , C12Y203/01009 , C12Y207/01036 , C12Y401/01033 , C12Y402/03027 , C12Y503/03002 , Y02E50/343
摘要： The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.
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公开(公告)号：US08569026B2
公开(公告)日：2013-10-29
申请号：US12560366
申请日：2009-09-15
摘要： The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells. The invention provides isoprene compositions, such as compositions with increased amount of isoprene or increased purity. Additionally, the invention provides methods of producing isoprene by culturing cells under conditions suitable for isoprene production while maintaining cell viability and/or metabolic activity.
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