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公开(公告)号：US08592194B2
公开(公告)日：2013-11-26
申请号：US12681612
申请日：2008-04-08
申请人： Wolfgang Aehle , Richard R. Bott , Igor Nikolaev , Martijn Scheffers , Piet Van Solingen , Casper Vroemen
发明人： Wolfgang Aehle , Richard R. Bott , Igor Nikolaev , Martijn Scheffers , Piet Van Solingen , Casper Vroemen
CPC分类号： C12N5/10
摘要： The present invention relates to variant glucoamylases wherein the variant has altered properties (e.g., improved thermostability and/or specific activity) compared to a corresponding parent glucoamylase. The present invention also relates to enzyme compositions comprising a variant glucoamylase (e.g., starch hydrolyzing compositions); DNA constructs comprising polynucleotides encoding the variants and methods of producing the variant glucoamylases in host cells.
摘要翻译： 本发明涉及变体葡糖淀粉酶，其中与相应的母体葡糖淀粉酶相比，变体具有改变的性质（例如改善的热稳定性和/或比活性）。 本发明还涉及包含变体葡糖淀粉酶（例如淀粉水解组合物）的酶组合物; 包含编码变体的多核苷酸的DNA构建体和在宿主细胞中产生变体葡糖淀粉酶的方法。
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公开(公告)号：US20130102035A1
公开(公告)日：2013-04-25
申请号：US13806398
申请日：2011-06-30
申请人： Peter Edvard Degn , Richard R. Bott , Casper Willem Vroemen , Martijn Silvan Scheffers , Wolfgang Aehle , Elin Petersen
发明人： Peter Edvard Degn , Richard R. Bott , Casper Willem Vroemen , Martijn Silvan Scheffers , Wolfgang Aehle , Elin Petersen
CPC分类号： C12N9/2408 , A23L29/35 , C12C5/004 , C12C7/04 , C12N9/2428 , C12P19/02 , C12P19/20 , C12Y302/01003 , C13K1/06 , Y02P20/52
摘要： The present invention relates to combinatorial variants of a parent glucoamylase that have altered properties for reducing the synthesis of condensation products during hydrolysis of starch. Accordingly the variants of a parent glucoamylase are suitable such as for use within brewing and glucose syrup production. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
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3.发明授权Composition comprising various proteorhodopsins and/or bacteriorhodopsins and use thereof 有权包含各种蛋白质视紫红质和/或细菌视紫红质的组合物及其用途公开(公告)号：US07982000B2
公开(公告)日：2011-07-19
申请号：US11569862
申请日：2005-06-09
申请人： Richard R. Bott , Rasmus B. Jensen , Bradley R. Kelemen , Donald E. Ward, II , Gregory M. Whited
发明人： Richard R. Bott , Rasmus B. Jensen , Bradley R. Kelemen , Donald E. Ward, II , Gregory M. Whited
IPC分类号： C07K17/00 , G11B11/00 , G11B7/085 , G11B7/00 , G11B5/84 , G11B7/24 , B32B3/02 , G03F7/00 , G02F1/09
CPC分类号： G11C7/00 , C07K14/215 , C09D11/037 , C09D11/50 , G11B7/242 , Y10S430/146 , Y10T428/249921 , Y10T428/31623 , Y10T428/31685 , Y10T428/31725 , Y10T428/31783
摘要： The present invention provides a solid material comprising an immobilized mixture of two or more proteorhodopsins, two or more bacteriorhodopsins, or one or more bacteriorhodopsin and one or more proteorhodopsins. The proteorhodopsins are selected from the group consisting of all-trans-retinal-containing proteorhodopsins and retinal analog-containing proteorhodopsins; all of which have absorption spectra that do not overlap. The bacteriorhodopsins are selected from the group consisting of all-trans-retinal-containing bacteriorhodopsins and retinal analog-containing bacteriorhodopsins; all of which have absorption spectra that do not overlap. The present invention also provides an optical information carrier, such as an optical data storage material and a fraud-proof optical data carrier, comprising the above-described solid material and a substrate selected from the group consisting of glass, paper, metal, fabric material, and plastic material, wherein said solid material is deposited on said substrate. The present invention further provides security ink comprising one or more hydrophilic polymers and a mixture of various photochromic materials.
摘要翻译： 本发明提供了一种固体材料，其包含两种或多种蛋白质视紫红质，两种或更多种细菌视紫红质，或一种或多种细菌视紫红质和一种或多种蛋白质视紫质的固定化混合物。 蛋白质视紫红质选自全反式视黄醛的蛋白质视黄素和含视网膜类似物的蛋白质视紫红质; 所有这些都具有不重叠的吸收光谱。 细菌视紫红质选自全反式视网膜含细菌视紫红质和视网膜类似物的细菌视紫红质; 所有这些都具有不重叠的吸收光谱。 本发明还提供一种光学信息载体，例如光学数据存储材料和防欺诈光学数据载体，其包括上述固体材料和选自玻璃，纸，金属，织物材料的基材 和塑料材料，其中所述固体材料沉积在所述基底上。 本发明还提供了包含一种或多种亲水聚合物和各种光致变色材料的混合物的安全油墨。
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公开(公告)号：US20110091958A1
公开(公告)日：2011-04-21
申请号：US12776921
申请日：2010-05-10
CPC分类号： C12N9/54 , C11D3/386 , C11D3/38609 , C12Y304/21062
摘要： Novel enzyme variants including protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 27, 45, 170, 181, 251 and 271 of Bacillus amyloliquefaciens subtilisin. Additional variants comprising at least one additional substituion at a positon selected from 1, 14, 49, 61, 87, 100, 102, 118, 128, 204 and 258 of Bacillus amyloliquefaciens subtilisin are also described.
摘要翻译： 公开了包含衍生自天然或重组非人蛋白酶的DNA序列的蛋白酶变体的新型酶变体。 一般来说，变体蛋白酶通过体外修饰编码天然存在或重组蛋白酶的前体DNA序列获得，以产生前体蛋白酶的氨基酸序列中的多个氨基酸残基的取代。 这种变体蛋白酶具有与前体蛋白酶不同的性质，例如改变的洗涤性能。 取代的氨基酸残基对应于解淀粉芽孢杆菌枯草杆菌蛋白酶的位置27,45,170,181,251和271。 还描述了在解淀粉芽孢杆菌枯草杆菌蛋白酶的1,4,14,69,61,87,100,102,118,128,204和258的选择位置中至少包含一个另外的取代基的另外的变体。
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公开(公告)号：US20110020899A1
公开(公告)日：2011-01-27
申请号：US12681612
申请日：2008-04-08
申请人： Wolfgang Aehle , Richard R. Bott , Igor Nikolaev , Martijn Scheffers , Piet Van Solingen , Casper Vroemen
发明人： Wolfgang Aehle , Richard R. Bott , Igor Nikolaev , Martijn Scheffers , Piet Van Solingen , Casper Vroemen
CPC分类号： C12N5/10
摘要： The present invention relates to variant glucoamylases wherein the variant has altered properties (e.g., improved thermostability and/or specific activity) compared to a corresponding parent glucoamylase. The present invention also relates to enzyme compositions comprising a variant glucoamylase (e.g., starch hydrolyzing compositions); DNA constructs comprising polynucleotides encoding the variants and methods of producing the variant glucoamylases in host cells
摘要翻译： 本发明涉及变体葡糖淀粉酶，其中与相应的母体葡糖淀粉酶相比，变体具有改变的性质（例如改善的热稳定性和/或比活性）。 本发明还涉及包含变体葡糖淀粉酶（例如淀粉水解组合物）的酶组合物; 包含编码变体的多核苷酸的DNA构建体和在宿主细胞中产生变体葡糖淀粉酶的方法
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公开(公告)号：US20090275080A1
公开(公告)日：2009-11-05
申请号：US12292563
申请日：2008-11-20
CPC分类号： C12N9/2428 , C12P7/06 , C12P19/02 , C12P19/14 , Y02E50/17
摘要： The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
摘要翻译： 本公开涉及具有改变的性质（例如改善的热稳定性和/或比活性）的母体葡糖淀粉酶的变体。 特别地，本公开提供了包含变体葡糖淀粉酶的组合物，包括淀粉水解组合物和清洁组合物。 本公开还涉及编码在宿主细胞中产生葡糖淀粉酶变体的变体和方法的DNA构建体。
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公开(公告)号：US07250281B2
公开(公告)日：2007-07-31
申请号：US10323324
申请日：2002-12-18
CPC分类号： C11D3/38609 , C12N9/54
摘要： Novel carbonyl hydrolase variants derived from the DNA sequences of naturally-occurring or recombinant non-human carbonyl hydrolases are disclosed. The variant carbonyl hydrolases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant carbonyl hydrolase to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor carbonyl hydrolase. Such variant carbonyl hydrolases have properties which are different from those of the precursor hydrolase, such as altered proteolytic activity, altered stability, etc. The substituted amino acid residues correspond to positions +76 in combination with one or more of the following residues +99, +101, +103, +104, +107, +123, +27, +105, +109, +126, +128, +135, +156, +166, +195, +197, +204, +206, +210, +216, +217, +218, +222, +260, +265 and/or +274 in Bacillus amyloliquefaciens subtilisin.
摘要翻译： 公开了衍生自天然存在或重组非人羰基水解酶的DNA序列的新型羰基水解酶变体。 变体羰基水解酶通常通过体外修饰编码天然存在或重组羰基水解酶的前体DNA序列获得，以产生前体羰基水解酶的氨基酸序列中的多个氨基酸残基的取代。 这种变体羰基水解酶具有与前体水解酶不同的性质，例如改变的蛋白水解活性，改变的稳定性等。取代的氨基酸残基对应于与一个或多个以下残基组合的位置+76， +101，+103，+104，+107，+123，+27，+105，+ 109，+126，+128，+135，+ 156，+166，+ 195，+ 197，+ 204，+ 206 ，+210，+ 216，+ 217，+ 218，+ 222，+ 260，+ 265和/或+ 274的解淀粉芽孢杆菌枯草杆菌蛋白酶。
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8.发明授权
公开(公告)号：US6090567A
公开(公告)日：2000-07-18
申请号：US445464
申请日：1995-05-22
申请人： Edward Benjamin Jakobovits , Joy L. Silen , Mark J. Levy , Thomas C. Goodman , Martin Becker , Edwin F. Ullman , Robert M. Caldwell , Richard R. Bott , Christopher Charles Barnett
发明人： Edward Benjamin Jakobovits , Joy L. Silen , Mark J. Levy , Thomas C. Goodman , Martin Becker , Edwin F. Ullman , Robert M. Caldwell , Richard R. Bott , Christopher Charles Barnett
IPC分类号： G01N33/542 , C12N9/04 , C12N15/09 , C12Q1/28 , C12Q1/32 , C12R1/19 , G01N33/535 , G01N33/569 , G01N33/58 , G01N33/78 , G01N33/94 , G01N33/53
CPC分类号： C12N9/0006 , C12Q1/28 , G01N33/535 , G01N33/56911 , G01N33/581 , G01N33/78 , G01N33/94 , G01N2333/904
摘要： Methods for immunoassay of analytes employing mutant glucose-6-phosphate dehydrogenase (G6PDH) enzymes as labels. In particular, the invention relates to the use of conjugates of an analyte or analyte analog and a mutant NAD.sup.+ dependent G6PDH differing from any precursor G6PDH by the deletion, substitution, or insertion, or any combination thereof of at least one amino acid per subunit. The invention also involves the construction of several mutations in precursor glucose-6-phosphate dehydrogenase (G6PDH) enzymes. Typically, the mutations involve deletion or substitution of one or more lysine residues, or introduction of one or more cysteine residues by insertion of cysteine to precursor G6PDH or substitution of precursor G6PDH amino acids residues with cysteine. The present invention also relates to conjugates of the subject enzymes and specific binding pair members, kits useful in performing the methods of the invention, cell lines producing the subject enzymes, DNA sequences encoding the subject enzymes, and vectors containing DNA encoding the subject enzymes and designed to allow a host cell to produce the subject enzymes.
摘要翻译： 使用突变型葡萄糖-6-磷酸脱氢酶（G6PDH）酶作为标记免疫测定分析物的方法。 特别地，本发明涉及通过每个亚基的至少一个氨基酸的缺失，取代或插入或其任何组合，使用不同于任何前体G6PDH的分析物或分析物类似物和突变体NAD +依赖性G6PDH的缀合物的用途。 本发明还涉及前体葡萄糖-6-磷酸脱氢酶（G6PDH）酶的几个突变的构建。 通常，突变涉及一个或多个赖氨酸残基的缺失或取代，或通过将半胱氨酸插入前体G6PDH或用半胱氨酸取代前体G6PDH氨基酸残基引入一个或多个半胱氨酸残基。 本发明还涉及本发明的酶和特异性结合对成员的缀合物，可用于实施本发明方法的试剂盒，产生本发明酶的细胞系，编码本发明酶的DNA序列，和含有编码本发明酶的DNA的载体和 旨在使宿主细胞产生目标酶。
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公开(公告)号：US5346823A
公开(公告)日：1994-09-13
申请号：US36592
申请日：1993-03-24
申请人： David A. Estell , James A. Wells , Richard R. Bott
发明人： David A. Estell , James A. Wells , Richard R. Bott
CPC分类号： C12R1/07 , C11D3/386 , C12N15/102 , C12N15/75 , C12N9/54
摘要： There are provided methods for making a mutant Bacillus subtilisin having altered oxidative stability, the methods comprising obtaining DNA fragment consisting of a region coding for a Bacillus subtilisin, and introducing a mutation into said DNA fragment such that the mutation is introduced in a region encoding a methionine, tryptophan, cysteine or lysine, sensitive to oxidation, such that upon expression of the mutant subtilisin one or more codon regions encoding for methionine, tryptophan, cysteine or lysine is replaced with an amino acid other than methionine, tryptophan, cysteine or lysine, preferably alanine or serine.
摘要翻译： 提供了制备具有改变的氧化稳定性的突变型枯草杆菌蛋白酶的方法，所述方法包括获得由编码枯草杆菌蛋白酶的区域组成的DNA片段，并将突变引入所述DNA片段，使得突变引入到编码 敏感的蛋氨酸，色氨酸，半胱氨酸或赖氨酸，使得突变型枯草杆菌蛋白酶表达后，编码甲硫氨酸，色氨酸，半胱氨酸或赖氨酸的一个或多个密码子区域被甲硫氨酸，色氨酸，半胱氨酸或赖氨酸以外的氨基酸替代， 优选丙氨酸或丝氨酸。
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公开(公告)号：USRE34606E
公开(公告)日：1994-05-10
申请号：US556918
申请日：1990-07-20
申请人： David A. Estell , James A. Wells , Richard R. Bott
发明人： David A. Estell , James A. Wells , Richard R. Bott
CPC分类号： C12R1/07 , C11D3/386 , C12N15/102 , C12N15/75 , C12N9/54
摘要： A cloned subtilsin gene has been modified at specific sites to cause amino acid substitutions at certain spots in the enzyme. The modified enzyme, preferably produced by Bacillus, is useful in combination with detergents.
摘要翻译： 已经在特定位点修饰了克隆的subtilisin基因，以在酶中的某些点处引起氨基酸取代。 优选由芽孢杆菌产生的修饰酶可与洗涤剂组合使用。
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