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公开(公告)号：US20130260432A1
公开(公告)日：2013-10-03
申请号：US13436612
申请日：2012-03-30
申请人： Richard R. BOTT , Marguerite A. Cervin , James T. Kellis, JR. , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Christopher Lee Rife , Derek H. Wells , Walter Weyler , Gregory M. Whited
发明人： Richard R. BOTT , Marguerite A. Cervin , James T. Kellis, JR. , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Christopher Lee Rife , Derek H. Wells , Walter Weyler , Gregory M. Whited
CPC分类号： C12N9/88 , C12P5/007 , C12Y402/03027
摘要： The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
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2.发明授权公开(公告)号：US08173410B2
公开(公告)日：2012-05-08
申请号：US12429143
申请日：2009-04-23
申请人： Richard R. Bott , Marguerite A. Cervin , James T. Kellis, Jr. , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Christopher Lee Rife , Derek H. Wells , Walter Weyler , Gregory M. Whited
发明人： Richard R. Bott , Marguerite A. Cervin , James T. Kellis, Jr. , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Christopher Lee Rife , Derek H. Wells , Walter Weyler , Gregory M. Whited
CPC分类号： C12N9/88 , C12P5/007 , C12Y402/03027
摘要： The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
摘要翻译： 本发明提供包含至少一种具有改善的催化活性和/或溶解度的异戊二烯合酶的方法和组合物。 特别地，本发明提供用于在微生物宿主细胞中增加异戊二烯生产的变体植物异戊二烯合酶。 本发明的生物合成生产的异戊二烯可用于制造橡胶和弹性体。
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3.发明授权公开(公告)号：US08916370B2
公开(公告)日：2014-12-23
申请号：US13436612
申请日：2012-03-30
申请人： Richard R. Bott , Marguerite A. Cervin , James T. Kellis, Jr. , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Christopher Lee Rife , Derek H. Wells , Walter Weyler , Gregory M. Whited
发明人： Richard R. Bott , Marguerite A. Cervin , James T. Kellis, Jr. , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Christopher Lee Rife , Derek H. Wells , Walter Weyler , Gregory M. Whited
IPC分类号： C12N9/88 , C12P21/06 , C12N15/00 , C12P5/02 , C12N1/20 , C12N1/00 , C07H21/04 , C07K1/00 , C12P5/00
CPC分类号： C12N9/88 , C12P5/007 , C12Y402/03027
摘要： The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
摘要翻译： 本发明提供包含至少一种具有改善的催化活性和/或溶解度的异戊二烯合酶的方法和组合物。 特别地，本发明提供用于在微生物宿主细胞中增加异戊二烯生产的变体植物异戊二烯合酶。 本发明的生物合成生产的异戊二烯可用于制造橡胶和弹性体。
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4.发明授权公开(公告)号：US08518686B2
公开(公告)日：2013-08-27
申请号：US12765825
申请日：2010-04-22
申请人： Zachary Quinn Beck , Richard R. Bott , Christopher Lee Rife , Derek H. Wells , Jeffrey V. Miller
发明人： Zachary Quinn Beck , Richard R. Bott , Christopher Lee Rife , Derek H. Wells , Jeffrey V. Miller
CPC分类号： C12P5/007 , C07K2299/00 , C12N9/88 , C12Y402/03027
摘要： The present invention provides a three-dimensional structures of P. tremuloides isoprene synthase and P. alba isoprene synthase. The invention also provides methods of using the three-dimensional structure to design isoprene synthases with improved activity for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
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5.发明申请THREE-DIMENSIONAL STRUCTURE OF ISOPRENE SYNTHASE AND ITS USE THEREOF FOR GENERATING VARIANTS 有权异丙肾上腺素的三维结构及其用于产生变异体公开(公告)号：US20110076743A1
公开(公告)日：2011-03-31
申请号：US12765825
申请日：2010-04-22
申请人： Zachary Quinn Beck , Richard R. Bott , Christopher Lee Rife , Derek H. Wells , Jeffrey V. Miller
发明人： Zachary Quinn Beck , Richard R. Bott , Christopher Lee Rife , Derek H. Wells , Jeffrey V. Miller
CPC分类号： C12P5/007 , C07K2299/00 , C12N9/88 , C12Y402/03027
摘要： The present invention provides a three-dimensional structures of P. tremuloides isoprene synthase and P. alba isoprene synthase. The invention also provides methods of using the three-dimensional structure to design isoprene synthases with improved activity for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
摘要翻译： 本发明提供了三叶虫异戊二烯合酶和异戊二烯异戊二烯合酶的三维结构。 本发明还提供使用三维结构设计异戊二烯合酶的方法，所述异戊二烯合酶具有改进的微生物宿主细胞中异戊二烯生产增加的活性。 本发明的生物合成生产的异戊二烯可用于制造橡胶和弹性体。
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