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公开(公告)号：US08095323B2
公开(公告)日：2012-01-10
申请号：US12692729
申请日：2010-01-25
CPC分类号： C07H21/04 , B01J2219/00432 , B01J2219/00608 , B01J2219/00626 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C12Q1/6827 , C12Q1/6874 , C40B40/06 , C40B60/14 , G06F19/20 , G06F19/22 , Y10T436/143333 , C12Q2565/501 , C12Q2525/179 , C12Q2525/204 , C12Q2525/185 , C12Q2535/131
摘要： The present invention generally provides a rapid efficient method for analyzing polymorphic or biallelic markers, and arrays for carrying out these analyses. In general, the methods of the present invention employ arrays of oligonucleotide probes that are complementary to target nucleic acids which correspond to the marker sequences of an individual. The probes are typically arranged in detection blocks, each block being capable of discriminating the three genotypes for a given marker, i.e., the heterozygote or either of the two homozygotes. The method allows for rapid, automatable analysis of genetic linkage to even complex polygenic traits.
摘要翻译： 本发明通常提供用于分析多态性或双重标记的快速有效的方法和用于进行这些分析的阵列。 通常，本发明的方法使用与对应于个体的标记序列的靶核酸互补的寡核苷酸探针阵列。 探针通常被布置在检测块中，每个区块能够区分给定标记，即杂合子或两个纯合子中的任一个的三种基因型。 该方法允许对甚至复杂的多基因性状的遗传连锁的快速，自动化分析。
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公开(公告)号：US07674587B2
公开(公告)日：2010-03-09
申请号：US11675980
申请日：2007-02-16
CPC分类号： C07H21/04 , B01J2219/00432 , B01J2219/00608 , B01J2219/00626 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C12Q1/6827 , C12Q1/6874 , C40B40/06 , C40B60/14 , G06F19/20 , G06F19/22 , Y10T436/143333 , C12Q2565/501 , C12Q2525/179 , C12Q2525/204 , C12Q2525/185 , C12Q2535/131
摘要： The present invention generally provides a rapid efficient method for analyzing polymorphic or biallelic markers, and arrays for carrying out these analyses. In general, the methods of the present invention employ arrays of oligonucleotide probes that are complementary to target nucleic acids which correspond to the marker sequences of an individual. The probes are typically arranged in detection blocks, each block being capable of discriminating the three genotypes for a given marker, i.e., the heterozygote or either of the two homozygotes. The method allows for rapid, automatable analysis of genetic linkage to even complex polygenic traits.
摘要翻译： 本发明通常提供用于分析多态性或双重标记的快速有效的方法和用于进行这些分析的阵列。 通常，本发明的方法使用与对应于个体的标记序列的靶核酸互补的寡核苷酸探针阵列。 探针通常被布置在检测块中，每个区块能够区分给定标记，即杂合子或两个纯合子中的任一个的三种基因型。 该方法允许对甚至复杂的多基因性状的遗传连锁的快速，自动化分析。
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3.发明申请Capturing sequences adjacent to type IIs restriction sites for genomic library mapping 审中-公开捕获与II型限制性位点相邻的序列用于基因组文库作图公开(公告)号：US20120258868A1
公开(公告)日：2012-10-11
申请号：US13159626
申请日：2011-06-14
CPC分类号： C12Q1/683 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6855 , C12Q1/6869 , C12Q1/6874 , C12Q2565/501 , C12Q2600/156 , C12Q2525/191 , C12Q2521/313 , C12Q2525/179 , C12Q2525/161 , C12Q2525/155 , C12Q2525/143 , C12Q2525/131
摘要： The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the “capturing” and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic mapping, screening and manipulation.
摘要翻译： 本发明涉及使用II型限制性内切核酸酶对多核苷酸序列中的遗传标记进行测序和鉴定的新方法。 本文描述的方法导致捕获和确定位于II型限制性位点附近的特异性寡核苷酸序列。 所得序列可用作用于遗传图谱，筛选和操纵的有效标记。
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4.发明申请Capturing Sequences Adjacent to type-IIs restriction sites for Genomic Library Mapping 失效捕获序列与基因组图谱绘制的II型限制性位点相邻公开(公告)号：US20100261616A1
公开(公告)日：2010-10-14
申请号：US12272680
申请日：2008-11-17
CPC分类号： C12Q1/683 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6855 , C12Q1/6869 , C12Q1/6874 , C12Q2565/501 , C12Q2600/156 , C12Q2525/191 , C12Q2521/313 , C12Q2525/179 , C12Q2525/161 , C12Q2525/155 , C12Q2525/143 , C12Q2525/131
摘要： The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the “capturing” and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic mapping, screening and manipulation.
摘要翻译： 本发明涉及使用II型限制性内切核酸酶对多核苷酸序列中的遗传标记进行测序和鉴定的新方法。 本文描述的方法导致“捕获”和确定与II型限制性位点相邻的特定寡核苷酸序列。 所得序列可用作用于遗传图谱，筛选和操纵的有效标记。
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5.发明授权Capturing sequences adjacent to type-IIS restriction sites for genomic library mapping 有权捕获与基因组文库映射的类型-Ill限制位点相邻的序列公开(公告)号：US07662559B2
公开(公告)日：2010-02-16
申请号：US10942479
申请日：2004-09-16
IPC分类号： C12Q1/68
CPC分类号： C12Q1/683 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6855 , C12Q1/6869 , C12Q1/6874 , C12Q2565/501 , C12Q2600/156 , C12Q2525/191 , C12Q2521/313 , C12Q2525/179 , C12Q2525/161 , C12Q2525/155 , C12Q2525/143 , C12Q2525/131
摘要： The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the “capturing” and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic mapping, screening and manipulation.
摘要翻译： 本发明涉及使用II型限制性内切核酸酶对多核苷酸序列中的遗传标记进行测序和鉴定的新方法。 本文描述的方法导致“捕获”和确定与II型限制性位点相邻的特定寡核苷酸序列。 所得序列可用作用于遗传图谱，筛选和操纵的有效标记。
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6.发明授权Methods for analyzing nucleic acids using a type IIs restriction endonuclease 失效使用II型限制性内切核酸酶分析核酸的方法公开(公告)号：US06509160B1
公开(公告)日：2003-01-21
申请号：US09940868
申请日：2001-08-27
IPC分类号： C12Q168
CPC分类号： C12Q1/683 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6855 , C12Q1/6869 , C12Q1/6874 , C12Q2565/501 , C12Q2600/156 , C12Q2525/191 , C12Q2521/313 , C12Q2525/179 , C12Q2525/161 , C12Q2525/155 , C12Q2525/143 , C12Q2525/131
摘要： The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the “capturing” and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic mapping, screening and manipulation.
摘要翻译： 本发明涉及使用II型限制性内切核酸酶对多核苷酸序列中的遗传标记进行测序和鉴定的新方法。 本文描述的方法导致“捕获”和确定与II型限制性位点相邻的特定寡核苷酸序列。 所得序列可用作用于遗传图谱，筛选和操纵的有效标记。
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7.发明授权Nucleic acid adapters containing a type IIs restriction site and methods of using the same 有权含有II型限制性位点的核酸衔接子及其使用方法公开(公告)号：US06291181B1
公开(公告)日：2001-09-18
申请号：US09412246
申请日：1999-10-05
IPC分类号： C12Q168
CPC分类号： C12Q1/683 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6855 , C12Q1/6869 , C12Q1/6874 , C12Q2565/501 , C12Q2600/156 , C12Q2525/191 , C12Q2521/313 , C12Q2525/179 , C12Q2525/161 , C12Q2525/155 , C12Q2525/143 , C12Q2525/131
摘要： The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the “capturing” and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic mapping, screening and manipulation.
摘要翻译： 本发明涉及使用II型限制性内切核酸酶对多核苷酸序列中的遗传标记进行测序和鉴定的新方法。 本文描述的方法导致“捕获”和确定与II型限制性位点相邻的特定寡核苷酸序列。 所得序列可用作用于遗传图谱，筛选和操纵的有效标记。
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8.发明授权Nucleic acid adapters containing a type IIs restriction site and methods of using the same 失效含有II型限制性位点的核酸衔接子及其使用方法
公开(公告)号：US6027894A
公开(公告)日：2000-02-22
申请号：US8094
申请日：1998-01-16
IPC分类号： C12Q1/68
CPC分类号： C12Q1/683 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6855 , C12Q1/6869 , C12Q1/6874 , C12Q2565/501 , C12Q2600/156
摘要： The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the "capturing" and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic mapping, screening and manipulation.
摘要翻译： 本发明涉及使用II型限制性内切核酸酶对多核苷酸序列中的遗传标记进行测序和鉴定的新方法。 本文描述的方法导致“捕获”和确定与II型限制性位点相邻的特定寡核苷酸序列。 所得序列可用作用于遗传图谱，筛选和操纵的有效标记。
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9.发明授权Capturing sequences adjacent to type-IIs restriction sites for genomic library mapping 失效捕获与II型限制性位点相邻的基因组文库作图的序列公开(公告)号：US07985547B2
公开(公告)日：2011-07-26
申请号：US12272680
申请日：2008-11-17
IPC分类号： C12Q1/68
CPC分类号： C12Q1/683 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6855 , C12Q1/6869 , C12Q1/6874 , C12Q2565/501 , C12Q2600/156 , C12Q2525/191 , C12Q2521/313 , C12Q2525/179 , C12Q2525/161 , C12Q2525/155 , C12Q2525/143 , C12Q2525/131
摘要： The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the “capturing” and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic mapping, screening and manipulation.
摘要翻译： 本发明涉及使用II型限制性内切核酸酶对多核苷酸序列中的遗传标记进行测序和鉴定的新方法。 本文描述的方法导致“捕获”和确定与II型限制性位点相邻的特定寡核苷酸序列。 所得序列可用作用于遗传图谱，筛选和操纵的有效标记。
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公开(公告)号：US5858659A
公开(公告)日：1999-01-12
申请号：US563762
申请日：1995-11-29
CPC分类号： C12Q1/6837 , B01J19/0046 , C12Q1/6827 , B01J2219/00529 , B01J2219/00608 , B01J2219/00626 , B01J2219/00659 , B01J2219/00695 , B01J2219/00722 , C40B40/06
摘要： The present invention generally provides a rapid efficient method for analyzing polymorphic or biallelic markers, and arrays for carrying out these analyses. In general, the methods of the present invention employ arrays of oligonucleotide probes that are complementary to target nucleic acids which correspond to the marker sequences of an individual. The probes are typically arranged in detection blocks, each block being capable of discriminating the three genotypes for a given marker, e.g., the heterozygote or either of the two homozygotes. The method allows for rapid, automatable analysis of genetic linkage to even complex polygenic traits.
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