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    Alphavirus vectors having attentuated virion structural proteins
    1.
    发明申请
    Alphavirus vectors having attentuated virion structural proteins 审中-公开
    具有病毒粒子结构蛋白的甲病毒载体

    公开(公告)号：US20060099587A1

    公开(公告)日：2006-05-11

    申请号：US10517083

    申请日：2003-06-20

    申请人： Robert Johnston ,  Nancy Davis ,  Ande West ,  Jonathan Smith

    发明人： Robert Johnston ,  Nancy Davis ,  Ande West ,  Jonathan Smith

    IPC分类号： C12Q1/68 ,  C12N7/00 ,  C12N7/01

    CPC分类号： C07K14/005 ,  A61K2039/5154 ,  A61K2039/5254 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/54 ,  C12N15/86 ,  C12N2740/16222 ,  C12N2770/36122 ,  C12N2770/36143

    摘要： The present invention provides immunogenic compositions and methods that may be used to administer safer (i.e., attenuated) alphavirus vectors (such as alphavirus vectors comprising a VEE virion shell) that retain improved immunogenicity as compared with other attenuated alphaviruses (e.g., the VEE 3014 mutant, described below). In particular embodiments of the invention, the alphavirus vector comprises VEE structural proteins comprising an attenuating mutation in the E1 glycoprotein. In other particular embodiments, the attenuating mutation is in the fusogenic region of the E1 glycoprotein. The present invention enables administration of lower dosages of a safer (i.e., attenuated) virus and, thus, can further reduce manufacturing costs. The present inventors have found that immunogenicity of alphavirus vectors may be influenced by a number of factors including species, site and route of administration.

    摘要翻译： 本发明提供免疫原性组合物和方法，其可用于与其他减毒的甲病毒（例如，VEE 3014突变体）相比，其保持更高（即减毒）的甲病毒载体（例如包含VEE病毒粒子的甲病毒载体），其保持改善的免疫原性 ， 如下面所描述的）。 在本发明的具体实施方案中，甲病毒载体包含在E1糖蛋白中包含减毒突变的VEE结构蛋白。 在其它具体实施方案中，减毒突变位于E1糖蛋白的融合区。 本发明使得能够施用较低剂量的更安全（即，减毒）的病毒，因此可以进一步降低制造成本。 本发明人已经发现甲病毒载体的免疫原性可能受许多因素的影响，包括物种，部位和给药途径。

    Alphavirus vectors and virosomes with modified HIV genes for use in vaccines
    2.
    发明授权
    Alphavirus vectors and virosomes with modified HIV genes for use in vaccines 有权
    甲病毒载体和具有修饰的HIV基因的病毒体用于疫苗

    公开(公告)号：US06783939B2

    公开(公告)日：2004-08-31

    申请号：US09991258

    申请日：2001-11-16

    申请人： Robert Olmsted ,  Paula Keith ,  Sergey Dryga ,  Ian Caley ,  Maureen Maughan ,  Robert Johnston ,  Nancy Davis ,  Ronald Swanstrom

    发明人： Robert Olmsted ,  Paula Keith ,  Sergey Dryga ,  Ian Caley ,  Maureen Maughan ,  Robert Johnston ,  Nancy Davis ,  Ronald Swanstrom

    IPC分类号： C12Q168

    CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K39/21 ,  A61K2039/5256 ,  A61K2039/5258 ,  A61K2039/54 ,  A61K2039/545 ,  A61K2039/575 ,  C12N7/00 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2740/16222 ,  C12N2740/16234 ,  C12N2770/36143 ,  C12N2770/36151

    摘要： The present invention provides methods and compositions comprising a population of alphavirus replicon particles comprising two or more isolated nucleic acids selected from 1) an isolated nucleic acid encoding an env gene product or an immunogenic fragment thereof of a human immunodeficiency virus, 2) an isolated nucleic acid encoding a g&agr;g gene product or an immunogenic fragment thereof of a human immunodeficiency virus, wherein the g&agr;g gene product or immunogenic fragment thereof is modified to inhibit formation of virus-like particles containing the g&agr;g gene product or the immunogenic fragment thereof and their release from a cell, and 3) an isolated nucleic acid encoding a pol gene product or an immunogenic fragment thereof of a human immunodeficiency virus, wherein the pol gene product or immunogenic fragment thereof is modified to inhibit protease, integrase, RNase H and/or reverse transcriptase activity, and wherein the nucleic acids are each contained within a separate alphavirus replicon particle.

    摘要翻译： 本发明提供方法和组合物，其包含包含两种或多种分离的核酸的甲型病毒复制子颗粒群，所述分离的核酸选自1）编码人免疫缺陷病毒的env基因产物或其免疫原性片段的分离的核酸，2）分离的核酸 编码人免疫缺陷病毒的galphag基因产物或其免疫原性片段的酸，其中所述galphag基因产物或其免疫原性片段被修饰以抑制含有galphag基因产物或其免疫原性片段的病毒样颗粒的形成及其从 细胞，以及3）编码人免疫缺陷病毒的pol基因产物或其免疫原性片段的分离的核酸，其中pol基因产物或其免疫原性片段被修饰以抑制蛋白酶，整合酶，RNA酶H和/或逆转录酶 活性，并且其中所述核酸各自包含在分离物内 食用甲病毒复制子颗粒。

    Scaling content communicated over a network
    4.
    发明授权
    Scaling content communicated over a network 有权
    通过网络扩展内容

    公开(公告)号：US08406134B2

    公开(公告)日：2013-03-26

    申请号：US12823202

    申请日：2010-06-25

    申请人： Douglas Medina ,  Frank Coppa ,  Robert Johnston

    发明人： Douglas Medina ,  Frank Coppa ,  Robert Johnston

    IPC分类号： G01R31/08

    CPC分类号： H04L1/0003 ,  H04L1/0002 ,  H04L1/0009 ,  H04L1/0014 ,  H04L1/0076 ,  H04L41/0604 ,  H04L41/0672 ,  H04L41/0681 ,  H04L41/5025 ,  H04L43/0823 ,  H04L43/16 ,  H04L47/11 ,  H04L47/38 ,  H04L65/605

    摘要： The disclosed subject matter relates to an architecture that can scale content resolution in order to mitigate errors in a provisioned service of a communication network, such as a wireless service or a femtocell service that integrates with DSL or other broadband carriers. The architecture can identify fault conditions relating to e.g., bandwidth oversubscription or symbolization integrity. Based upon such identification, the architecture can alter encoding format codecs of certain types of content in order to reduce their resolution/quality, thereby mitigating bandwidth oversubscription fault conditions or freeing up space (without necessarily increasing bandwidth) to insert additional FEC code.

    摘要翻译： 所公开的主题涉及可以缩放内容分辨率以便减轻诸如与DSL或其他宽带载波集成的无线服务或毫微微小区服务的通信网络的提供服务中的错误的架构。 该架构可以识别与例如带宽超额订阅或符号化完整性有关的故障状况。 基于这种识别，架构可以改变某些类型的内容的编码格式编解码器，以便降低它们的分辨率/质量，从而减轻带宽过量订阅故障条件或释放空间（不一定增加带宽）以插入附加FEC码。

    TEMPERATURE MEASUREMENT MEANS FOR COOKING APPLIANCES
    5.
    发明申请
    TEMPERATURE MEASUREMENT MEANS FOR COOKING APPLIANCES 有权
    温度测量仪器用于烹饪器具

    公开(公告)号：US20090165774A1

    公开(公告)日：2009-07-02

    申请号：US12344325

    申请日：2008-12-26

    申请人： Robert Johnston ,  Joe Andrews

    发明人： Robert Johnston ,  Joe Andrews

    IPC分类号： A47J37/07

    CPC分类号： A47J37/0682 ,  A47J27/62 ,  A47J37/0713

    摘要： A cooking appliance having a heating source and a cooking surface disposed above the heating source. A temperature sensing probe is located between the heating source and the cooking surface for indicating to a user the temperature at or near the cooking surface.

    摘要翻译： 一种具有加热源和设置在加热源上方的烹饪表面的烹饪器具。 温度感测探针位于加热源和烹饪表面之间，用于向使用者指示烹饪表面处或附近的温度。

    Injection Moulded Container
    6.
    发明申请
    Injection Moulded Container 失效
    注塑容器

    公开(公告)号：US20080289980A1

    公开(公告)日：2008-11-27

    申请号：US10590981

    申请日：2006-03-13

    申请人： Robert Johnston

    发明人： Robert Johnston

    IPC分类号： B65D85/57

    CPC分类号： B29C45/2673 ,  B29C33/3828 ,  B29C33/3842 ,  B29C33/56

    摘要： A method of making an injection moulded container for housing information storage media, e.g. a DVD, the container having a base portion (10) and a lid portion (11) moveable about a spine portion (12) between open and closed configurations and holding means (15) for holding the media within the container, the method involving the use of an injection moulding tool comprising two carrier units (40, 41) each adapted to receive a plurality of inserts therein which define a cavity for forming the container: a first set of the inserts (50-59) for forming a first set of features of the container being formed of a first material, e.g. steel, and a second set of. inserts (42-45) for forming a second set of features of the container being formed of a second material, e.g a copper alloy, the first set of inserts (50-59) being machined to the required shape for fitting within the carrier units (40, 41) and for forming the first set of features and the second set of inserts (42-45) being machined so as to fit within the carrier units (40, 41) but one or more of the second set of inserts (42-45) being formed with excess material on surfaces thereof defining said second set of features pending a decision as to the precise shape of said second set of features, whereby once a decision has been made the tool can be completed by machining said excess material to the required shape and assembling the inserts in the mould.

    摘要翻译： 制造用于容纳信息存储介质的注塑容器的方法，例如 DVD，具有基部（10）的容器和可在打开和关闭构造之间围绕脊部（12）移动的盖部分（11）和用于将介质保持在容器内的保持装置（15），该方法涉及 使用包括两个承载单元（40,41）的注射模制工具，每个承载单元适于容纳其中限定用于形成容器的空腔的多个插入物：第一组插入件（50-59），用于形成第一组 容器的特征由第一材料形成，例如， 钢，第二套。 用于形成由第二材料（例如铜合金）形成的容器的第二组特征的插入件（42-45），所述第一组插入件（50-59）被加工成所需的形状，用于装配在所述承载单元 （40,41）并且用于形成第一组特征，并且第二组插入件（42-45）被机加工以便装配在承载单元（40,41）内，但是第二组插入件 42-45）在限定所述第二组特征的表面上形成有多余的材料，等待关于所述第二组特征的精确形状的决定，由此一旦作出决定，可以通过机加工所述多余材料来完成所述工具 到所需的形状并将插入件组装在模具中。

    N-CARBOBENZYLOXY (N-CBZ)-DEPROTECTING ENZYME AND USES THEREFOR
    8.
    发明申请
    N-CARBOBENZYLOXY (N-CBZ)-DEPROTECTING ENZYME AND USES THEREFOR 失效
    N-CARBOBENZYLOXY（N-CBZ）-EPROTECTING ENZYME AND USES THEREFOR

    公开(公告)号：US20060149047A1

    公开(公告)日：2006-07-06

    申请号：US10453418

    申请日：2003-06-03

    申请人： Venkata Nanduri ,  Ramesh Patel ,  Steven Goldberg ,  Robert Johnston

    发明人： Venkata Nanduri ,  Ramesh Patel ,  Steven Goldberg ,  Robert Johnston

    IPC分类号： C07H21/04

    CPC分类号： C12N9/78

    摘要： This invention relates to isolated or recombinant N-CBZ-deprotecting enzyme polypeptides that catalyze the removal of CBZ from CBZ-protected amino acids and alcohols, and variants, modifications, and fragments thereof. Also related are isolated nucleic acids encoding N-CBZ-deprotecting enzyme polypeptides, and variants, modifications, and fragments thereof, as well as vectors and host cells comprising these nucleic acids. The invention also relates to methods of obtaining isolated nucleic acids, polypeptides, and antibodies, and methods of using polypeptides in various reactions for industrial or pharmaceutical applications.

    摘要翻译： 本发明涉及分离或重组的N-CBZ脱保护酶多肽，其催化从CBZ保护的氨基酸和醇及其变体，修饰和片段中除去CBZ。 还涉及编码N-CBZ-脱保护酶多肽的分离的核酸及其变体，修饰和片段，以及包含这些核酸的载体和宿主细胞。 本发明还涉及获得分离的核酸，多肽和抗体的方法，以及在工业或药物应用的各种反应中使用多肽的方法。

    Migrating integration processes among data integration platforms
    10.
    发明申请
    Migrating integration processes among data integration platforms 审中-公开
    在数据集成平台之间迁移集成过程

    公开(公告)号：US20050243604A1

    公开(公告)日：2005-11-03

    申请号：US11083064

    申请日：2005-03-16

    申请人： Richard Harken ,  Robert Johnston

    发明人： Richard Harken ,  Robert Johnston

    IPC分类号： G06F9/44 ,  G11C16/06

    CPC分类号： G06F8/51

    摘要： Methods and systems are provided for migrating a data integration facility, such as an ETL job, from a source data integration platform to a target data integration platform. For example, systems and methods are provided for migrating a data integration job from a source data integration platform having a source native format to a target data integration platform having a target native format; wherein the target native format is different than the source native format. The systems and methods may involve analyzing a source language construct of the source data integration platform to determine a logical syntax; constructing a target language construct of the target data integration platform adapted to perform the same logical operation on the target data integration platform as the source language construct performs on the source data integration platform; and substituting the target language construct for the source language construct in the source code for the data integration job.

    摘要翻译： 提供了方法和系统，用于将数据集成工具（如ETL作业）从源数据集成平台迁移到目标数据集成平台。 例如，提供系统和方法用于将数据集成作业从具有源原始格式的源数据集成平台迁移到具有目标本机格式的目​​标数据集成平台; 其中目标本机格式不同于源本机格式。 系统和方法可以涉及分析源数据集成平台的源语言结构以确定逻辑语法; 构建目标数据集成平台的目标语言结构，适用于在源数据集成平台上对源数据集成平台执行与源数据集成平台相同的逻辑运算; 并将目标语言结构替换为数据集成作业的源代码中的源语言结构。