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公开(公告)号：US07981659B2
公开(公告)日：2011-07-19
申请号：US12083539
申请日：2006-09-25
IPC分类号： C12N1/20
CPC分类号： C12N15/75 , C12N9/2417 , C12N9/54 , C12P21/02
摘要： Novel Bacillus subtilis mutant strains having good productivity of various enzymes are provided through extensive analysis of strains that are derived from Bacillus subtilis via gene disruption. The Bacillus subtilis mutant strains according to the present invention have genomic structures prepared by deletion of regions listed in the columns for deficient regions. Each of these Bacillus subtilis mutant strains exerts significantly improved secretory productivity of a protein when a gene encoding such a secretory target protein is introduced so that it can be expressed, compared with a case in which the same gene is introduced into a wild-type strain.
摘要翻译： 通过对通过基因破坏衍生自枯草芽孢杆菌的菌株的广泛分析，提供具有各种酶的良好生产力的新型枯草芽孢杆菌突变菌株。 根据本发明的枯草芽孢杆菌突变菌株具有通过缺失缺陷区列中列出的区域而制备的基因组结构。 当与将相同基因导入野生型菌株的情况相比，当引入编码这种分泌型靶蛋白的基因以使其能够被表达时，这些枯草芽孢杆菌突变菌株中的每一种都能显着提高蛋白质的分泌生产力 。
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公开(公告)号：US20090221055A1
公开(公告)日：2009-09-03
申请号：US12083539
申请日：2006-09-25
IPC分类号： C12N1/21
CPC分类号： C12N15/75 , C12N9/2417 , C12N9/54 , C12P21/02
摘要： Novel Bacillus subtilis mutant strains having good productivity of various enzymes are provided through extensive analysis of strains that are derived from Bacillus subtilis via gene disruption. The Bacillus subtilis mutant strains according to the present invention have genomic structures prepared by deletion of regions listed in the columns for deficient regions. Each of these Bacillus subtilis mutant strains exerts significantly improved secretory productivity of a protein when a gene encoding such a secretory target protein is introduced so that it can be expressed, compared with a case in which the same gene is introduced into a wild-type strain.
摘要翻译： 通过对通过基因破坏衍生自枯草芽孢杆菌的菌株的广泛分析，提供具有各种酶的良好生产力的新型枯草芽孢杆菌突变菌株。 根据本发明的枯草芽孢杆菌突变菌株具有通过缺失缺陷区列中列出的区域而制备的基因组结构。 当与将相同基因导入野生型菌株的情况相比，当引入编码这种分泌型靶蛋白的基因以使其能够被表达时，这些枯草芽孢杆菌突变菌株中的每一种都能显着提高蛋白质的分泌生产力 。
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公开(公告)号：US08778650B2
公开(公告)日：2014-07-15
申请号：US13266525
申请日：2010-04-26
CPC分类号： C11D3/38618 , C11D3/386 , C11D3/38681 , C12N9/54
摘要： The invention is directed to an alkaline protease that has the amino acid sequence of SEQ ID NO: 2 or of an amino acid sequence having an identity of 90% or more thereto, except that one or more amino acid residues at positions selected from (a) position 6, (b) position 15, (c) position 16, (d) position 65, (e) position 66, (f) position 82, (g) position 83, (h) position 204, (i) position 319, and (j) position 337 of SEQ ID NO: 2, or at positions corresponding thereto, are substituted with certain other amino acid residues. The invention is also directed to detergent compositions and culture medium that contain the protease.
摘要翻译： 本发明涉及具有SEQ ID NO：2的氨基酸序列或90％以上的氨基酸序列的碱性蛋白酶，除了选自（a）的位置的一个或多个氨基酸残基 ）位置6，（b）位置15，（c）位置16，（d）位置65，（e）位置66，（f）位置82，（g）位置83，（h）位置204，（i） 319和（j）SEQ ID NO：2的位置337或与其相应的位置被某些其它氨基酸残基取代。 本发明还涉及含有蛋白酶的洗涤剂组合物和培养基。
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公开(公告)号：US20120058928A1
公开(公告)日：2012-03-08
申请号：US13266525
申请日：2010-04-26
CPC分类号： C11D3/38618 , C11D3/386 , C11D3/38681 , C12N9/54
摘要： An alkaline protease variant derived from an alkaline protease consisting of an amino acid sequence represented by SEQ ID NO: 2 or consisting of an amino acid sequence having an identity of 90% or more therewith, which variant has mutations wherein one or more amino acid residues at positions selected from (a) position 6, (b) position 15, (c) position 16, (d) position 65, (e) position 66, (f) position 82, (g) position 83, (h) position 204, (i) position 319, and (j) position 337 of the amino acid sequence represented by SEQ ID NO: 2, or at positions corresponding thereto are substituted with the following amino acid residues: (a) or a position corresponding thereto: Typ, Leu, Val, Ile, Met, Tyr, Gln, Lys, Thr, Phe, Arg, Ser, Cys, Ala, or His; (b) or a position corresponding thereto: Glu, Met, Asp, Val, Gln, Arg, Cys, Trp, Ala, or Phe; (c) or a position corresponding thereto: Met, Glu, Arg, Val, Lys, Phe, Tyr, Ile, His, Asp, or Cys; (d) or a position corresponding thereto: Trp; (e) or a position corresponding thereto: His, Trp, Ser, or Leu; (f) or a position corresponding thereto: Ala, Glu, Gln, Ser, Cys, Gly, H is, Lys, Arg, Met, or Asn; (g) or a position corresponding thereto: Ala, Ser, or Cys; (h) or a position corresponding thereto: Glu, Asp, Cys, Val, Thr, Pro, His, Ile, Trp, Ser, Asn, Lys, or Arg; (i) or a position corresponding thereto: Trp, Val, Thr, Leu, Ile, Cys, Glu, Lys, Tyr, Arg, Phe, Gln, Met, Pro, Asp, Asn, H is, or Ser; and (j) or a position corresponding thereto: Arg, Gly, Ser, Lys, Gln, Thr, His, Ala, Cys, or Val.
摘要翻译： 由碱性蛋白酶衍生的碱性蛋白酶变体，其由SEQ ID NO：2所示的氨基酸序列组成或由与其同一性为90％以上的氨基酸序列组成，所述变体具有突变，其中一个或多个氨基酸残基 在从（a）位置6，（b）位置15，（c）位置16，（d）位置65，（e）位置66，（f）位置82，（g）位置83，（h） 204，（i）位置319和（j）由SEQ ID NO：2表示的氨基酸序列的位置337或与其相对应的位置被以下氨基酸残基取代：（a）或与其对应的位置： Typ，Leu，Val，Ile，Met，Tyr，Gln，Lys，Thr，Phe，Arg，Ser，Cys，Ala或His; （b）或相应位置：Glu，Met，Asp，Val，Gln，Arg，Cys，Trp，Ala或Phe; （c）或对应的位置：Met，Glu，Arg，Val，Lys，Phe，Tyr，Ile，His，Asp或Cys; （d）或与之对应的位置：Trp; （e）或与之对应的位置：His，Trp，Ser或Leu; （f）或对应位置：Ala，Glu，Gln，Ser，Cys，Gly，H is，Lys，Arg，Met或Asn; （g）或对应的位置：Ala，Ser或Cys; （h）或相应位置：Glu，Asp，Cys，Val，Thr，Pro，His，Ile，Trp，Ser，Asn，Lys或Arg; （i）或与其相对应的位置：Trp，Val，Thr，Leu，Ile，Cys，Glu，Lys，Tyr，Arg，Phe，Gln，Met，Pro，Asp，Asn，H is或Ser; 和（j）或对应的位置：Arg，Gly，Ser，Lys，Gln，Thr，His，Ala，Cys或Val。
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公开(公告)号：US07585674B2
公开(公告)日：2009-09-08
申请号：US10479214
申请日：2002-05-28
申请人： Kazuhisa Sawada , Keiji Endo , Tadahiro Ozawa , Masatoshi Tohata , Katsuya Ozaki
发明人： Kazuhisa Sawada , Keiji Endo , Tadahiro Ozawa , Masatoshi Tohata , Katsuya Ozaki
CPC分类号： C07K14/32 , C07K2319/00 , C12N1/20
摘要： A microorganism wherein one or more genes selected from the group of genes participating in sporulation in the middle to late stages of sporulation have been deleted or inactivated; and a process for producing a target product (a protein) by use the microorganism. No spore is formed when this microorganism is employed, thereby enabling production of a target product (a protein) while decreasing energy loss, production of a by-product and specific production speed to decrease unnecessary consumption of a medium. Moreover, the production period can be prolonged, whereby the target product (the protein) can be produced efficiently.
摘要翻译： 一种微生物，其中一个或多个选自参与孢子形成中期至晚期孢子形成的基因的基因已被删除或失活; 以及通过使用微生物生产目标产物（蛋白质）的方法。 当使用该微生物时不形成孢子，从而能够减少能量损失，副产物的产生和特定的生产速度，从而能够生产目标产物（蛋白质），从而减少不必要的培养基消耗。 此外，可以延长生产周期，由此有效地生产目标产物（蛋白质）。
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公开(公告)号：US20110151567A1
公开(公告)日：2011-06-23
申请号：US12530135
申请日：2008-04-08
申请人： Shenghao Liu , Keiji Endo , Katsutoshi Ara
发明人： Shenghao Liu , Keiji Endo , Katsutoshi Ara
CPC分类号： C12P21/02
摘要： A recombinant microorganism having improved productivity for a protein or a polypeptide, and a method for producing a protein or a polypeptide using the recombinant microorganism, are provided. A recombinant microorganism obtained by transfecting a gene for encoding a desired protein or polypeptide into a microorganism strain which is obtained by genetically constructing to overexpress secY gene of Bacillus subtilis or a gene corresponding to the secY gene, and deleting or inactivating one or more genes selected from sporulation-associated genes and genes corresponding to the sporulation-associated genes from the genome.
摘要翻译： 提供了具有提高的蛋白质或多肽的生产力的重组微生物，以及使用该重组微生物生产蛋白质或多肽的方法。 将通过将所需蛋白质或多肽编码的基因转染到通过遗传构建以过表达枯草芽孢杆菌的secY基因或相应于所述secY基因的基因而获得的微生物菌株获得的重组微生物，以及删除或灭活所选择的一种或多种基因 来自与来自基因组的孢子形成相关基因相对应的孢子形成相关基因和基因。
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公开(公告)号：US20100255534A1
公开(公告)日：2010-10-07
申请号：US12528039
申请日：2007-12-27
申请人： Keiji Endo , Katsutoshi Ara
发明人： Keiji Endo , Katsutoshi Ara
摘要： Provision of a recombinant microorganism which has increased productivity of a protein or polypeptide of interest and a method for producing a protein or polypeptide of interest using the recombinant microorganism. The recombinant microorganism is produced by transferring a gene encoding a protein or polypeptide of interest to a microorganism strain, wherein the microorganism strain is prepared by: introducing a transcription initiation regulatory region that functions in the microorganism or both the transcription initiation regulatory region and a ribosome-binding site that functions in the microorganism into the upstream of a Bacillus subtilis prsA gene or a gene corresponding thereto in the genome of a parental microorganism, or introducing a gene fragment prepared by ligating a transcription initiation regulatory region that functions in the microorganism or both the transcription initiation regulatory region and a ribosome-binding site that functions in the microorganism to the upstream of the Bacillus subtilis prsA gene or a gene corresponding thereto into the genome of a parental microorganism; and deleting or inactivating one or more genes selected from an abrB gene, a dltA gene, a dltB gene, a dltC gene, a dltD gene, a dltE gene, and a gene (genes) corresponding thereto.
摘要翻译： 提供具有增加的目的蛋白质或多肽的生产力的重组微生物以及使用重组微生物产生感兴趣的蛋白质或多肽的方法。 通过将编码目的蛋白质或多肽的基因转移到微生物菌株来产生重组微生物，其中微生物菌株通过以下方式制备：引入在微生物中起作用的转录起始调节区或转录起始调节区和核糖体 在微生物中起作用的枯草芽孢杆菌prsA基因或与亲本微生物基因组相对应的基因的上游的引入位点，或引入通过连接在微生物中起作用的转录起始调节区或两者兼有的基因片段 转录起始调节区和在微生物中起作用的枯草芽孢杆菌prsA基因上游的核糖体结合位点或与之相对应的基因转化为亲本微生物的基因组; 以及删除或失活一个或多个选自abrB基因，dltA基因，dltB基因，dltC基因，dltD基因，dltE基因和与其相对应的基因（基因）的基因。
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8.发明授权Recombinant microorganism that expresses a secY gene with deletion of sporulation-associated genes and method of producing thereof 有权表达具有孢子形成相关基因缺失的secY基因的重组微生物及其制备方法公开(公告)号：US08389264B2
公开(公告)日：2013-03-05
申请号：US12530135
申请日：2008-04-08
申请人： Shenghao Liu , Keiji Endo , Katsutoshi Ara
发明人： Shenghao Liu , Keiji Endo , Katsutoshi Ara
IPC分类号： C12N1/21
CPC分类号： C12P21/02
摘要： A recombinant microorganism having improved productivity for a protein or a polypeptide, and a method for producing a protein or a polypeptide using the recombinant microorganism, are provided.A recombinant microorganism obtained by transfecting a gene for encoding a desired protein or polypeptide into a microorganism strain which is obtained by genetically constructing to overexpress secY gene of Bacillus subtilis or a gene corresponding to the secY gene, and deleting or inactivating one or more genes selected from sporulation-associated genes and genes corresponding to the sporulation-associated genes from the genome.
摘要翻译： 提供了具有提高的蛋白质或多肽的生产力的重组微生物，以及使用该重组微生物生产蛋白质或多肽的方法。 将通过将所需蛋白质或多肽编码的基因转染到通过遗传构建以过表达枯草芽孢杆菌的secY基因或相应于所述secY基因的基因而获得的微生物菌株获得的重组微生物，以及删除或灭活所选择的一种或多种基因 来自与来自基因组的孢子形成相关基因相对应的孢子形成相关基因和基因。
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公开(公告)号：US08293516B2
公开(公告)日：2012-10-23
申请号：US12528039
申请日：2007-12-27
申请人： Keiji Endo , Katsutoshi Ara
发明人： Keiji Endo , Katsutoshi Ara
摘要： A recombinant microorganism is provided that has increased productivity of a protein or polypeptide of interest. A method for producing a protein of interest using the microorganism is also provided. The microorganism is prepared by inserting, in its genome, a transcription initiation regulatory region or both a transcription initiation regulatory region and a ribosome-binding site, upstream of a Bacillus subtilis prsA gene and by deleting or inactivating one or more of the abrB gene, dltA gene, dltB gene, dltC gene, dltD gene, dltE gene, or a gene (genes) corresponding thereto.
摘要翻译： 提供了具有增加的感兴趣的蛋白质或多肽的生产力的重组微生物。 还提供了使用该微生物生产感兴趣的蛋白质的方法。 通过在其基因组中插入转录起始调节区或在枯草芽孢杆菌prsA基因的上游插入转录起始调节区和核糖体结合位点，以及通过缺失或失活一个或多个abrB基因， dltA基因，dltB基因，dltC基因，dltD基因，dltE基因或与其对应的基因（基因）。
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公开(公告)号：US20090029417A1
公开(公告)日：2009-01-29
申请号：US12279271
申请日：2007-02-15
申请人： Keiji Endo , Shenghao Liu , Katsutoshi Ara
发明人： Keiji Endo , Shenghao Liu , Katsutoshi Ara
CPC分类号： C12N9/2437
摘要： The object of the invention is to provide a microorganism which enhances productivity of cellulase and is useful for industrial production of cellulase, and to provide a method for producing cellulase by use of the microorganism. The present invention provides a recombinant microorganism produced by transferring a gene encoding cellulase to a parental microorganism which has been genetically modified so as to overexpress a Bacillus subtilis secY gene or a gene corresponding thereto.
摘要翻译： 本发明的目的是提供一种提高纤维素酶生产率并可用于纤维素酶的工业生产的微生物，并提供使用微生物生产纤维素酶的方法。 本发明提供了一种通过将编码纤维素酶的基因转移到已被遗传修饰以使其过表达枯草芽孢杆菌SecY基因或相应基因的亲本微生物而产生的重组微生物。
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