公开(公告)号：US20220002709A1

公开(公告)日：2022-01-06

申请号：US17292941

申请日：2018-11-22

Applicant: SHIMADZU CORPORATION

Inventor： Seiya FUJIWARA ,  Masaki KANAI ,  Hiroyuki JIKUYA ,  Tetsuo OHASHI ,  Masamitsu SHIKATA ,  Ayaka MINAMIMOTO

IPC: C12N15/10 ,  B01J19/08 ,  B03C1/005 ,  B03C1/28

Abstract: A purpose of the present invention is to suppress reduction in recovery rate of a target component due to a gel adhering to an inner wall surface of a treatment liquid layer. After passing magnetic particles through a gel layer, when moving the magnetic particles in the treatment liquid layer adjacent to the gel layer, the magnetic force source is automatically operated in a longitudinal direction of a tubular container so that the magnetic particles do not enter a range of a certain distance from the gel layer through which the magnetic particles have passed.

公开(公告)号：US20210222154A1

公开(公告)日：2021-07-22

申请号：US17154525

申请日：2021-01-21

Applicant: SHIMADZU CORPORATION

Inventor： Akira MURAMATSU ,  Masamitsu SHIKATA ,  Masaki KANAI ,  Seiya FUJIWARA ,  Tetsuo OHASHI

IPC: C12N15/10 ,  G01N1/40 ,  C12Q1/6806

Abstract: The present invention relates to a method for operating magnetic particles in a device in which gel-like medium layer and liquid layer are alternately arranged inside the hollow tube and, first liquid layer and second liquid layer are separated by gel-like medium layer, and magnetic solid with large particle size exists in the first liquid layer rather than magnetic particles and the said magnetic particles. The method of the present invention comprises the first step of moving the magnetic particles and the magnetic solid in the first liquid layer to the gel-like medium layer, the second step of moving the magnetic particles and the magnetic solid moved into the gel-like medium layer into the second liquid layer, and the third step of inverting the movement of the magnetic particles and the magnetic solid moved into the second liquid layer into the gel-like medium layer, by operating a magnetic field.

公开(公告)号：US20210395840A1

公开(公告)日：2021-12-23

申请号：US17159783

申请日：2021-01-27

Applicant: SHIMADZU CORPORATION ,  National University Corporation Tokyo Medical and Dental University ,  Nihon Techno Service Co., Ltd. ,  National University Corporation Oita University ,  RIKEN ,  Toho University

Inventor： Masamitsu SHIKATA ,  Kenji NINOMIYA ,  Norio SHIMIZU ,  Hiroshi TAKASE ,  Manabu MOCHIZUKI ,  Yasuhiro TOMARU ,  Satoko NAKANO ,  Sunao SUGITA ,  Takashi SUZUKI

IPC: C12Q1/70 ,  C12Q1/689 ,  C12Q1/6893

Abstract: This invention provides a simple analysis method by a PCR method that simultaneously performs, in a single container, separating nucleic acid of pathogens contained in a tissue fragment and preparing a PCR buffer solution, and a kit used for the analysis method. A method for detecting a pathogen includes: (1) obtaining a liquid specimen mixture by adding a tissue fragment containing a pathogen to a PCR buffer solution containing a proteolytic enzyme; (2) heating the liquid specimen mixture at a first temperature; (3) further heating at a second temperature; (4) performing PCR by adding a portion of the liquid mixture obtained in (3) above to a solid composition for PCR reaction containing DNA polymerase and one or more kinds of PCR primer pair; and (5) detecting a PCR product generated in (4) above.

公开(公告)号：US20210031205A1

公开(公告)日：2021-02-04

申请号：US16942843

申请日：2020-07-30

Applicant: SHIMADZU CORPORATION

Inventor： Masamitsu SHIKATA ,  Kenji NINOMIYA ,  Naoko TAKAOKA ,  Shinichiro KOBAYASHI

IPC: B01L9/00 ,  G01N35/10

Abstract: A dispensing tip holder is used when a dispensing tip is attached to a tip of a pipette, and includes a peripheral wall having a shape surrounding an outer peripheral surface of the dispensing tip, and an insertion portion having an insertion opening into which the dispensing tip is to be inserted. The peripheral wall has a first end into which the dispensing tip is to be inserted, and has a second end provided at a position opposite to the first end. The insertion portion is formed in the peripheral wall such that an outer peripheral surface of the dispensing tip abuts against an edge of the insertion opening, and is formed in the peripheral wall such that a tip of the dispensing tip does not project from the second end of the peripheral wall, when the dispensing tip is inserted into the insertion opening from the first end.

公开(公告)号：US20170138824A1

公开(公告)日：2017-05-18

申请号：US15322527

申请日：2014-07-02

Applicant: SHIMADZU CORPORATION

Inventor： Nobuhiro HANAFUSA ,  Masamitsu SHIKATA

IPC: G01N1/10 ,  G01N1/34 ,  G01N30/06 ,  G01N1/14

CPC classification number: G01N1/10 ,  B01L3/502 ,  B01L2200/0631 ,  G01N1/14 ,  G01N1/34 ,  G01N1/4005 ,  G01N30/06 ,  G01N35/0099 ,  G01N35/025 ,  G01N2001/4011 ,  G01N2035/00485 ,  G01N2035/00534 ,  G01N2035/0427 ,  G01N2035/0441 ,  G01N2035/0443

Abstract: A preprocessing apparatus uses preprocessing kits prepared for samples, respectively, sets the preprocessing kits in plural processing ports provided so as to correspond to preprocessing items, and executes a predetermined preprocessing item. A controller which controls operations of a carrying mechanism holding and carrying the preprocessing kits, and processing parts executing the preprocessing items in the respective processing ports, includes a processing-state control part, a random access part, and a preprocessing part. The random access part is configured to check availability of a preprocessing port corresponding to a preprocessing item to be executed on a sample contained in a preprocessing kit, and set the preprocessing kit in a processing port if the processing port is available as a processing port corresponding to the preprocessing item. The preprocessing part executes a corresponding preprocessing item on a sample in a preprocessing kit when the preprocessing kit is set in the processing port.

公开(公告)号：US20240368681A1

公开(公告)日：2024-11-07

申请号：US18685768

申请日：2022-08-23

Applicant: SHIMADZU CORPORATION ,  NIHON TECHNO SERVICE CO., LTD.

Inventor： Masamitsu SHIKATA ,  Yasuhiro TOMARU

IPC: C12Q1/6851

Abstract: A method for quantifying DNA in a specimen includes: a step of performing PCR by adding, to a first container containing a specimen and a second container containing a known amount of standard DNA, the same number of copies of internal control DNA, a PCR primer pair for amplifying the DNA in the specimen and the standard DNA, a PCR primer pair for amplifying the internal control DNA, an oligonucleotide fluorescence-labeled probe, an oligonucleotide fluorescence-labeled probe, and a PCR buffer solution containing a DNA polymerase; a step of measuring a difference between Ct values in the PCR by comparing Ct values for the internal control DNA contained in the first container and the second container; a step of correcting a Ct value for the DNA in the specimen contained in the first container; and a step of measuring an amount of DNA in the specimen from a calibration curve.

公开(公告)号：US20220355310A1

公开(公告)日：2022-11-10

申请号：US17840568

申请日：2022-06-14

Applicant: SHIMADZU CORPORATION

Inventor： Masamitsu SHIKATA ,  Ayaka MINAMIMOTO ,  Akira MURAMATSU ,  Masaki KANAI ,  Seiya FUJIWARA ,  Tetsuo OHASHI ,  Hiroyuki JIKUYA

IPC: B03C1/12 ,  C12Q1/6865 ,  B01D15/38

Abstract: A manufacturing method of an operation pipe, which use a gel to perform operations such as separation, extraction, purification, elution, recovery, analysis and the like of target components that are biological components such as nucleic acids. More specifically, a manufacturing method of an operation pipe, with which it is possible to perform operations such as separation, extraction, purification, elution, recovery, analysis and the like of target components in a sealable pipe by operating magnetic particles in the pipe under a magnetic field from outside of the pipe.

公开(公告)号：US20210114018A1

公开(公告)日：2021-04-22

申请号：US16657336

申请日：2019-10-18

Applicant: SHIMADZU CORPORATION

Inventor： Naoko TAKAOKA ,  Masamitsu SHIKATA ,  Kenji NINOMIYA ,  Shinichiro KOBAYASHI

IPC: B01L3/00 ,  G01N21/03 ,  G01N21/64

Abstract: A reaction vessel has plural wells each configured to accommodate a sample on the same plane or on the same straight line. Among the wells of a well plate, a specific well other than a well at a center of point symmetry is an orientation recognizing well in which a fluorescent dye is placed. The reaction vessel can identify a position of the orientation recognizing well by using fluorescence emitted from the fluorescent dye, and can thereby recognize an orientation of the reaction vessel.

公开(公告)号：US20190381515A1

公开(公告)日：2019-12-19

申请号：US16401646

申请日：2019-05-02

Applicant: SHIMADZU CORPORATION

Inventor： Akira MURAMATSU ,  Masamitsu SHIKATA ,  Ayaka YAMANO

IPC: B03C1/28 ,  B03C1/01 ,  G01N33/543

Abstract: A magnet is disposed close to and outside a container held by a container holding part, and relatively moves along the container, so as to move the magnetic particles in the container by a magnetic force. A housing accommodates the magnet and the container holding part in the housing in a state where a position of the magnet relatively moving along the container is not visually recognizable from the outside. A display unit is provided on the housing so as to be visually recognizable from the outside and has light emitting regions that can emit light. A processing step execution unit sequentially executes a plurality of processing steps by relatively moving the magnet. When the plurality of processing steps are sequentially executed, a light emission control unit causes the light emitting regions to emit light according to a position of the magnet.

公开(公告)号：US20190022658A1

公开(公告)日：2019-01-24

申请号：US16040966

申请日：2018-07-20

Applicant: SHIMADZU CORPORATION

Inventor： Kenji NINOMIYA ,  Masamitsu SHIKATA ,  Naoko TAKAOKA ,  Shinichiro KOBAYASHI

IPC: B01L7/00

Abstract: A gene measurement apparatus includes: a temperature control block that is provided with, on its upper surface, a plurality of reaction vessel wells each being used to house a reaction vessel, and that performs temperature treatment on a specimen in the reaction vessel by controlling temperature of the plurality of reaction vessel wells using a heating element or a cooling element; and a reaction vessel detector configured to detect whether the reaction vessel is housed in each of the plurality of reaction vessel wells of the temperature control block before the temperature treatment is started.