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公开(公告)号：US20080087547A1
公开(公告)日：2008-04-17
申请号：US11905539
申请日：2007-10-02
申请人： Shin Nakamura , Rintaro Yamamoto , Akira Harada , Makoto Hazama , Takashi Ikegami , Toru Kaji , Naoya Endo , Hidesato Kumagai , Tetsuo Ohashi , Atsushi Inami , Keisuke Miyamoto , Shinichi Utsunomiya
发明人： Shin Nakamura , Rintaro Yamamoto , Akira Harada , Makoto Hazama , Takashi Ikegami , Toru Kaji , Naoya Endo , Hidesato Kumagai , Tetsuo Ohashi , Atsushi Inami , Keisuke Miyamoto , Shinichi Utsunomiya
IPC分类号： G01N27/447 , G01N21/64
CPC分类号： G01N27/44791 , G01N27/44721 , G01N27/44743 , G01N27/44782
摘要： For automating the operation of an electrophoresis apparatus and improving the throughput, the present electrophoresis apparatus has two platens capable of controlling temperature of electrophoresis plates placed thereon, a loading medium charging unit for sending a loading medium under pressure, a loading medium charging nozzle mechanism having a pair of nozzles connected to the loading medium charging unit, a pipetter mechanism for dispensing samples to sample dispensing openings of the electrophoresis plates placed on the platens, a stacker mechanism for storing sample plates, a loading buffer solution supplying mechanism, a loading buffer solution dispensing mechanism connected to the loading buffer solution supplying mechanism, a power unit for allowing electrophoresis separation for each electrophoresis plate placed on the platens, and a detector for optically detecting components migrating through each electrophoresis flow channel of the electrophoresis plates.
摘要翻译： 为了使电泳装置的操作自动化并提高通过量，本电泳装置具有能够控制放置在其上的电泳板的温度的两个压板，用于在压力下发送加载介质的装载介质充电单元，具有 连接到装载介质充电单元的一对喷嘴，用于分配样品以对放置在压板上的电泳板的分配开口进行采样的移液器机构，用于存储样品板的堆垛机构，装载缓冲溶液供应机构，装载缓冲溶液 连接到装载缓冲溶液供应机构的分配机构，用于允许放置在台板上的每个电泳板电泳分离的动力单元，以及用于光学检测通过电泳板的每个电泳流路移动的组分的检测器。
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公开(公告)号：US07655125B2
公开(公告)日：2010-02-02
申请号：US11905539
申请日：2007-10-02
申请人： Shin Nakamura , Rintaro Yamamoto , Akira Harada , Makoto Hazama , Takashi Ikegami , Toru Kaji , Naoya Endo , Hidesato Kumagai , Tetsuo Ohashi , Atsushi Inami , Keisuke Miyamoto , Shinichi Utsunomiya
发明人： Shin Nakamura , Rintaro Yamamoto , Akira Harada , Makoto Hazama , Takashi Ikegami , Toru Kaji , Naoya Endo , Hidesato Kumagai , Tetsuo Ohashi , Atsushi Inami , Keisuke Miyamoto , Shinichi Utsunomiya
IPC分类号： G01N27/453
CPC分类号： G01N27/44791 , G01N27/44721 , G01N27/44743 , G01N27/44782
摘要： For automating the operation of an electrophoresis apparatus and improving the throughput, the present electrophoresis apparatus has two platens capable of controlling temperature of electrophoresis plates placed thereon, a loading medium charging unit for sending a loading medium under pressure, a loading medium charging nozzle mechanism having a pair of nozzles connected to the loading medium charging unit, a pipetter mechanism for dispensing samples to sample dispensing openings of the electrophoresis plates placed on the platens, a stacker mechanism for storing sample plates, a loading buffer solution supplying mechanism, a loading buffer solution dispensing mechanism connected to the loading buffer solution supplying mechanism, a power unit for allowing electrophoresis separation for each electrophoresis plate placed on the platens, and a detector for optically detecting components migrating through each electrophoresis flow channel of the electrophoresis plates.
摘要翻译： 为了使电泳装置的操作自动化并提高通过量，本电泳装置具有能够控制放置在其上的电泳板的温度的两个压板，用于在压力下发送加载介质的装载介质充电单元，具有 连接到装载介质充电单元的一对喷嘴，用于分配样品以对放置在压板上的电泳板的分配开口进行采样的移液器机构，用于存储样品板的堆垛机构，装载缓冲溶液供应机构，装载缓冲溶液 连接到装载缓冲溶液供应机构的分配机构，用于允许放置在台板上的每个电泳板电泳分离的动力单元，以及用于光学检测通过电泳板的每个电泳流路移动的组分的检测器。
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公开(公告)号：US5395594A
公开(公告)日：1995-03-07
申请号：US933729
申请日：1992-08-24
IPC分类号： B01J19/00 , B01J19/28 , C07K1/04 , C07K5/08 , C07K7/06 , C07K14/575 , C07K14/655 , C07K14/70 , C40B40/10 , C40B60/14 , B01J8/02
CPC分类号： B01J19/004 , B01J19/0046 , C07K1/045 , B01J2219/00286 , B01J2219/00288 , B01J2219/00308 , B01J2219/00373 , B01J2219/00423 , B01J2219/00493 , B01J2219/005 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00725 , C40B40/10 , C40B60/14
摘要： The present invention is directed to a simultaneous multiple chemical synthesizer comprising a number of reaction vessels wherein each vessel has a filter in the bottom portion thereof, a number of needles wherein each needle is connected to an aspiration injection line of a reaction mixture and a gas supply line in connection with each reaction vessel and each needle does not touch the resin contained in the reaction vessel, a number of arms which are horizontally and vertically movable and hold the respective needles, a bubbling gas line and a waste discharge line in connection with each reaction vessel, wherein each line is connected to the bottom portion of each reaction vessel, a number of purging means which move synchronously with said waste discharge lines, and means for washing the portions of the needles and purge means which contact with the reaction reagents.
摘要翻译： 本发明涉及一种同时多重化学合成器，其包括多个反应容器，其中每个容器在其底部具有过滤器，多个针，其中每个针连接到反应混合物的抽吸注入管线和气体 与每个反应容器相关的供应管线，并且每个针不接触包含在反应容器中的树脂，可水平和垂直移动并保持各针的多个臂，鼓泡气管线和排放废水排放管线与 每个反应容器，其中每条管线连接到每个反应容器的底部，与所述废物排放管线同步移动的多个清洗装置，以及用于清洗针头的部分和与反应试剂接触的清洗装置的装置 。
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公开(公告)号：US5344613A
公开(公告)日：1994-09-06
申请号：US9080
申请日：1993-01-26
CPC分类号： B01L99/00 , B01J19/0046 , C07K1/045 , B01J2219/00286 , B01J2219/00288 , B01J2219/005 , B01J2219/0059 , B01J2219/00596 , B01J2219/00725 , C40B40/10 , C40B60/14
摘要： In an automated solid-phase peptide synthesizing apparatus comprising at least one reaction vessel containing particulate resin as an insoluble support matrix which anchors elongating peptide chains yielded through coupling-assembly formation of peptide bonds, mixing agitation of reagents, as well as circulation agitation of washing solvents flushing the coupling-associated reaction solutions, introduced in peptide chain-assembly process steps into the reaction vessel, is effected by the bubbling of an inert gas forcibly passed through the reagents or washing solvent via a drainage port of the reaction vessel, in order to promote peptide synthesis. A removable agitation stabilizer is inserted into the reaction vessel as a barrier for inhibiting extra-vessel escape, due to action of the bubbling inert gas, of the reagents as well as the washing solvents, and of the support matrix anchoring the elongating peptide chains. The stabilizer also breaks clumping and coagulative skinning of elongated peptides bound to the support matrix, as effects further consequent of the action of the bubbling inert gas and levitated thereby toward the mouth of the reaction vessel during peptide synthesis. The stabilizer additionally acts as a baffle furthering mixing agitation, to promote dispersive mixing of the peptidyl resin with the reagents and washing solvents during peptide chain assembly; and It also serves to distribute washing solvents evenly along the inner surface of the reaction vessel.
摘要翻译： 在一种自动化固相肽合成装置中，包括至少一个含有颗粒状树脂作为不溶性支持基质的反应容器，该反应容器通过肽键的偶联 - 组装形成固定延伸的肽链，混合搅拌试剂以及循环搅拌洗涤 将通过肽链组装工艺步骤引入反应容器的偶联相关反应溶液冲洗到反应容器中，通过以反应容器的排水口强制通过试剂或洗涤溶剂的惰性气体起泡 促进肽合成。 将可移除的搅拌稳定剂插入到反应容器中，作为阻止由于起泡惰性气体的作用以及洗涤溶剂和固定伸长肽链的载体基质而导致的外部容器逸出的屏障。 稳定剂还破坏结合到载体基质上的细长肽的结块和凝结性皮肤剥离，其作用是由于气泡惰性气体的作用而进一步引起，并且由此在肽合成期间悬浮于反应容器口中。 稳定剂另外作为进一步混合搅拌的挡板，以促进肽链组装期间肽基树脂与试剂和洗涤溶剂的分散混合; 并且还用于沿着反应容器的内表面均匀分布洗涤溶剂。
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公开(公告)号：US20100089771A1
公开(公告)日：2010-04-15
申请号：US12447253
申请日：2006-10-26
申请人： Shinichi Utsunomiya , Makoto Hazama
发明人： Shinichi Utsunomiya , Makoto Hazama
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , G01N27/44717 , C12Q2565/125 , C12Q2527/113
摘要： To enable accurate analysis of a base sequence even in an electrophoretic pattern containing a degraded part. The base sequence of a nucleic acid is determined by conducting the following steps (A) to (C) in this order: (A) a basic peak extracting step wherein basic peaks are extracted from electrophoretic data involving the respective peaks of the four bases obtained by electrophoresing a sample nucleic acid; (B) a condition determining step wherein a basic peak at the search starting point, from which the search is started, and a standard peak-to-peak distance are determined based on the time-series data composed of the basic peaks extracted above; and (C) a base sequence determining step wherein peak-to-peak intervals are successively scanned forward and backward in the above-described time-series data starting from the basic peak at the search starting point and then the peak-to-peak distance is compared with the standard peak-to-peak distance as determined above so as to add an interpolation peak to a peak-missing area.
摘要翻译： 即使在包含降解部分的电泳图案中也能准确分析碱基序列。 通过按照以下顺序进行以下步骤（A）至（C）来确定核酸的碱基序列：（A）基本峰提取步骤，其中从获得的四个碱基的各个峰的电泳数据中提取基本峰 通过电泳样品核酸; （B）条件确定步骤，其中基于由上述提取的基本峰值组成的时间序列数据来确定搜索起始点处的基本峰值，从其开始搜索和标准峰 - 峰距离; 和（C）基准序列确定步骤，其中峰 - 间隔在从搜索起点的基本峰开始的上述时间序列数据中向前和向后连续扫描，然后是峰 - 峰距离 与上面确定的标准峰 - 峰距离进行比较，以便将峰值添加到峰值缺失区域。
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公开(公告)号：US08155889B2
公开(公告)日：2012-04-10
申请号：US12517552
申请日：2006-12-04
申请人： Shinichi Utsunomiya , Makoto Hazama
发明人： Shinichi Utsunomiya , Makoto Hazama
IPC分类号： G01N33/50
CPC分类号： G01N27/447
摘要： Disclosed herein is a method for assessing the degree of reliability of a nucleic acid base sequence, by which a higher-accuracy assessment result of the degree of reliability can be obtained as compared to a case where the degree of reliability is assessed based on only the evaluation of analytical data. The method includes the steps: (A) when measured data is processed into analytical data, computing a processing evaluation value E1i for evaluating quality of measured data of an i-th base and contents of processing having been performed on the measured data of the i-th base; (B) computing an analytical data evaluation value E2i of the i-th base based on the processed analytical data; and (C) computing a degree of reliability by a predetermined calculation formula using the processing evaluation value E1i and the analytical data evaluation value E2i.
摘要翻译： 本文公开了一种用于评估核酸碱基序列的可靠性程度的方法，通过该方法可以获得与可靠性程度仅基于 分析数据评估。 该方法包括以下步骤：（A）当将测量数据处理为分析数据时，计算用于评估第i个基站的测量数据的质量的处理评估值E1i和已经对i的测量数据执行的处理内容 基地 （B）基于经处理的分析数据计算第i个基站的分析数据评估值E2i; 以及（C）使用处理评估值E1i和分析数据评价值E2i计算通过预定计算公式的可靠度。
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公开(公告)号：US20100070189A1
公开(公告)日：2010-03-18
申请号：US12517552
申请日：2006-12-04
申请人： Shinichi Utsunomiya , Makoto Hazama
发明人： Shinichi Utsunomiya , Makoto Hazama
IPC分类号： G06F19/00
CPC分类号： G01N27/447
摘要： Disclosed herein is a method for assessing the degree of reliability of a nucleic acid base sequence, by which a higher-accuracy assessment result of the degree of reliability can be obtained as compared to a case where the degree of reliability is assessed based on only the evaluation of analytical data. The method includes the steps: (A) when measured data is processed into analytical data, computing a processing evaluation value E1i for evaluating quality of measured data of an i-th base and contents of processing having been performed on the measured data of the i-th base; (B) computing an analytical data evaluation value E2i of the i-th base based on the processed analytical data; and (C) computing a degree of reliability by a predetermined calculation formula using the processing evaluation value E1i and the analytical data evaluation value E2i.
摘要翻译： 本文公开了一种用于评估核酸碱基序列的可靠性程度的方法，通过该方法可以获得与可靠性程度仅基于 分析数据评估。 该方法包括以下步骤：（A）当将测量数据处理为分析数据时，计算用于评估第i个基站的测量数据的质量的处理评估值E1i和已经对i的测量数据执行的处理内容 基地 （B）基于经处理的分析数据计算第i个基站的分析数据评估值E2i; 以及（C）使用处理评估值E1i和分析数据评价值E2i计算通过预定计算公式的可靠度。
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公开(公告)号：US08306756B2
公开(公告)日：2012-11-06
申请号：US12447253
申请日：2006-10-26
申请人： Shinichi Utsunomiya , Makoto Hazama
发明人： Shinichi Utsunomiya , Makoto Hazama
IPC分类号： G01N33/48
CPC分类号： C12Q1/6869 , G01N27/44717 , C12Q2565/125 , C12Q2527/113
摘要： To enable accurate analysis of a base sequence even in an electrophoretic pattern containing a degraded part. The base sequence of a nucleic acid is determined by conducting the following steps (A) to (C) in this order: (A) a basic peak extracting step wherein basic peaks are extracted from electrophoretic data involving the respective peaks of the four bases obtained by electrophoresing a sample nucleic acid; (B) a condition determining step wherein a basic peak at the search starting point, from which the search is started, and a standard peak-to-peak distance are determined based on the time-series data composed of the basic peaks extracted above; and (C) a base sequence determining step wherein peak-to-peak intervals are successively scanned forward and backward in the above-described time-series data starting from the basic peak at the search starting point and then the peak-to-peak distance is compared with the standard peak-to-peak distance as determined above so as to add an interpolation peak to a peak-missing area.
摘要翻译： 即使在包含降解部分的电泳图案中也能准确分析碱基序列。 通过按照以下顺序进行以下步骤（A）至（C）来确定核酸的碱基序列：（A）基本峰提取步骤，其中从获得的四个碱基的各个峰的电泳数据中提取基本峰 通过电泳样品核酸; （B）条件确定步骤，其中基于由上述提取的基本峰值组成的时间序列数据来确定搜索起始点处的基本峰值，从其开始搜索和标准峰 - 峰距离; 和（C）基准序列确定步骤，其中峰 - 间隔在从搜索起点的基本峰开始的上述时间序列数据中向前和向后连续扫描，然后是峰 - 峰距离 与上面确定的标准峰 - 峰距离进行比较，以便将峰值添加到峰值缺失区域。
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9.发明授权公开(公告)号：US07195698B2
公开(公告)日：2007-03-27
申请号：US10318124
申请日：2002-12-13
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： G01N27/44782 , G01N27/44743
摘要： An electrode plate of a sample plate is set on the body of an electrophoretic apparatus, while a plug is inserted into a migration high voltage line connection hole and connected to a high-tension distribution cable. Each well of a base plate is inserted into a through hole of a well guide and further press-fit and engaged into a cavity of an electrode plate, for fixing the base plate to the electrode plate. Thereafter a sample is introduced into each well of the base plate and an end of a capillary column is dipped into each well for applying a migration voltage and electrophoretically injecting the sample into the capillary column.
摘要翻译： 将样品板的电极板放置在电泳装置的主体上，同时将插头插入迁移高压线路连接孔并连接到高压配电电缆。 基板的每个孔插入井导向件的通孔中，并进一步压配合并接合到电极板的空腔中，用于将基板固定到电极板。 然后将样品引入基板的每个孔中，并将毛细管柱的末端浸入每个孔中以施加迁移电压并将样品电泳注入毛细管柱中。
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10.发明授权公开(公告)号：US06517696B1
公开(公告)日：2003-02-11
申请号：US09426760
申请日：1999-10-26
IPC分类号： C25B900
CPC分类号： G01N27/44782 , G01N27/44743
摘要： An electrode plate of a sample plate is set on the body of an electrophoretic apparatus, while a plug is inserted into a migration high voltage line connection hole and connected to a high-tension distribution cable. Each well of a base plate is inserted into a through hole of a well guide and further press-fit and engaged into a cavity of an electrode plate, for fixing the base plate to the electrode plate. Thereafter a sample is introduced into each well of the base plate and an end of a capillary column is dipped into each well for applying a migration voltage and electrophoretically injecting the sample into the capillary column.
摘要翻译： 将样品板的电极板放置在电泳装置的主体上，同时将插头插入迁移高压线路连接孔并连接到高压配电电缆。 基板的每个孔插入井导向件的通孔中，并进一步压配合并接合到电极板的空腔中，用于将基板固定到电极板。 然后将样品引入基板的每个孔中，并将毛细管柱的末端浸入每个孔中以施加迁移电压并将样品电泳注入毛细管柱中。
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