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1.发明授权公开(公告)号：US07195698B2
公开(公告)日：2007-03-27
申请号：US10318124
申请日：2002-12-13
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： G01N27/44782 , G01N27/44743
摘要： An electrode plate of a sample plate is set on the body of an electrophoretic apparatus, while a plug is inserted into a migration high voltage line connection hole and connected to a high-tension distribution cable. Each well of a base plate is inserted into a through hole of a well guide and further press-fit and engaged into a cavity of an electrode plate, for fixing the base plate to the electrode plate. Thereafter a sample is introduced into each well of the base plate and an end of a capillary column is dipped into each well for applying a migration voltage and electrophoretically injecting the sample into the capillary column.
摘要翻译： 将样品板的电极板放置在电泳装置的主体上，同时将插头插入迁移高压线路连接孔并连接到高压配电电缆。 基板的每个孔插入井导向件的通孔中，并进一步压配合并接合到电极板的空腔中，用于将基板固定到电极板。 然后将样品引入基板的每个孔中，并将毛细管柱的末端浸入每个孔中以施加迁移电压并将样品电泳注入毛细管柱中。
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2.发明授权公开(公告)号：US06517696B1
公开(公告)日：2003-02-11
申请号：US09426760
申请日：1999-10-26
IPC分类号： C25B900
CPC分类号： G01N27/44782 , G01N27/44743
摘要： An electrode plate of a sample plate is set on the body of an electrophoretic apparatus, while a plug is inserted into a migration high voltage line connection hole and connected to a high-tension distribution cable. Each well of a base plate is inserted into a through hole of a well guide and further press-fit and engaged into a cavity of an electrode plate, for fixing the base plate to the electrode plate. Thereafter a sample is introduced into each well of the base plate and an end of a capillary column is dipped into each well for applying a migration voltage and electrophoretically injecting the sample into the capillary column.
摘要翻译： 将样品板的电极板放置在电泳装置的主体上，同时将插头插入迁移高压线路连接孔并连接到高压配电电缆。 基板的每个孔插入井导向件的通孔中，并进一步压配合并接合到电极板的空腔中，用于将基板固定到电极板。 然后将样品引入基板的每个孔中，并将毛细管柱的末端浸入每个孔中以施加迁移电压并将样品电泳注入毛细管柱中。
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公开(公告)号：US06461492B1
公开(公告)日：2002-10-08
申请号：US09443719
申请日：1999-11-19
IPC分类号： C25F702
CPC分类号： G01N21/645 , G01N21/6428 , G01N27/44721
摘要： A multi-capillary electrophoresis apparatus arranged with a fixed sample injection holder opposite a detection side holder within the same plane and an epi-optical detection system. A sample is injected sequentially and separated components are successively fed to the detection part for analysis by fluorescence. The epi-optical system adjusts the parallelism between the detection side holder and scanning axis of the detector. Thus, a capillary electrophoretic apparatus can detect fluorescence from a fluorochrome bonded to samples as a label without influence by Raman scattering or Rayleigh scattering.
摘要翻译： 一种多毛细管电泳装置，其具有与同一平面内的检测侧支架相对的固定样品注射保持器和外延光学检测系统。 依次注入样品，并将分离的组分依次送入检测部分进行荧光分析。 外延光学系统调节检测器侧检测器与检测器的扫描轴之间的平行度。 因此，毛细管电泳装置可以在不受拉曼散射或瑞利散射影响的情况下检测到作为标签结合到样品的荧光染料的荧光。
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公开(公告)号：US6093300A
公开(公告)日：2000-07-25
申请号：US37005
申请日：1998-03-09
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44782 , G01N27/44743 , Y10S436/809
摘要： A base plate is made of an insulating material, and comprised of a flat surface and a connector part connected therewith. A plurality of wells are vertically and transversely arranged on the surface of the base plate at regular intervals respectively, and the respective wells are provided with individual electrode patterns reaching the connector part from bottoms thereof through the surface of the base plate. The connector part is connected to an external high-voltage application part.
摘要翻译： 基板由绝缘材料制成，并且由平坦表面和与其连接的连接器部件组成。 多个孔分别以规则的间隔垂直和横向地布置在基板的表面上，并且各孔具有从其底部通过基板的表面到达连接器部分的单独的电极图案。 连接器部分连接到外部高压施加部件。
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公开(公告)号：US06783650B2
公开(公告)日：2004-08-31
申请号：US10126661
申请日：2002-04-22
IPC分类号： C02F140
CPC分类号： G01N21/645 , G01N21/6428 , G01N27/44721
摘要： A capillary cassette is made up of two separated holders, an array of capillaries and a tube. The capillaries that make up the array are inserted into the first holder, extend through second holder having a detection window and then the capillary array is aligned and then bundled and inserted into a heat shrinkable tube which covers positions to which filler is applied, the tube is heated and shrunk to bundle them in an airtight manner and inserted into a mounting member.
摘要翻译： 毛细管盒由两个分离的保持器，一组毛细管和一个管构成。 构成阵列的毛细管被插入第一保持器中，延伸穿过具有检测窗口的第二保持器，然后将毛细管阵列对准，然后捆扎并插入到覆盖施加填料的位置的热收缩管中，管 被加热和收缩以将它们以气密的方式捆扎并插入到安装构件中。
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公开(公告)号：US06383356B1
公开(公告)日：2002-05-07
申请号：US09448139
申请日：1999-11-24
IPC分类号： G02N2726
CPC分类号： G01N27/44704
摘要： A drain joint of a pump block is opened, a piston (19a) is pushed while a piston (13a) is fixed, for charging a passage between a Luer-Lok joint (17) and an intersection part as well as a passage between the intersection part and the drain joint with a buffer. Thereafter the piston (13a) is pushed while the piston (19a) is fixed, to charge a passage (7a) with a polymer. Then, the drain joint is closed, the piston (13a) is pushed and the piston (19a) is pulled in response to the amount of pushing, for charging the passage between the intersection point and the Luer-Lok joint (17) with the polymer. Thereafter, the piston (13a) is pushed while the piston (19a) is fixed, to charge a capillary column with the polymer.
摘要翻译： 打开泵块的排水接头，同时在固定活塞（13a）的同时推动活塞（19a），用于对路德洛接头（17）和交叉部分之间的通道以及通道 交叉部分和排水接头与缓冲器。 此后，在活塞（19a）被固定的同时推动活塞（13a），以便用聚合物对通道（7a）充电。 然后，关闭排水接头，推动活塞（13a），并且响应于推动量而拉动活塞（19a），用于对交点和鲁尔洛克接头（17）之间的通道进行充电， 聚合物。 此后，在固定活塞（19a）的同时推动活塞（13a），以便与聚合物一起为毛细管柱充电。
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公开(公告)号：US6120667A
公开(公告)日：2000-09-19
申请号：US6933
申请日：1998-01-14
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44782 , G01N27/44721
摘要： After a sample stage moves and brings a sample titer plate toward and into contact with a capillary array end, a high voltage is applied across electrodes for a prescribed time for injecting samples. Thereafter an electrophoresis reservoir comes into contact with the capillary array end, for starting electrophoresis. When electrophoresed/separated DNA fragments pass through a detection part, an excitation/photoreceiving optical system is scanned so that photomultipliers detect fluorescence from four types of fluorescent materials labeling the samples. The excitation/photoreceiving optical system comprises an epi-optical system and a confocal optical system, and is scanned at a high speed.
摘要翻译： 在样品台移动并将样品滴定板朝向并与毛细管阵列端接触之后，在电极上施加高电压达预定时间以注射样品。 此后，电泳槽与毛细管阵列端接触，用于开始电泳。 当电泳/分离的DNA片段通过检测部分时，扫描激发/光接收光学系统，使得光电倍增管检测标记样品的四种类型的荧光材料的荧光。 激发/光接收光学系统包括外延光学系统和共焦光学系统，并且被高速扫描。
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公开(公告)号：US07655125B2
公开(公告)日：2010-02-02
申请号：US11905539
申请日：2007-10-02
申请人： Shin Nakamura , Rintaro Yamamoto , Akira Harada , Makoto Hazama , Takashi Ikegami , Toru Kaji , Naoya Endo , Hidesato Kumagai , Tetsuo Ohashi , Atsushi Inami , Keisuke Miyamoto , Shinichi Utsunomiya
发明人： Shin Nakamura , Rintaro Yamamoto , Akira Harada , Makoto Hazama , Takashi Ikegami , Toru Kaji , Naoya Endo , Hidesato Kumagai , Tetsuo Ohashi , Atsushi Inami , Keisuke Miyamoto , Shinichi Utsunomiya
IPC分类号： G01N27/453
CPC分类号： G01N27/44791 , G01N27/44721 , G01N27/44743 , G01N27/44782
摘要： For automating the operation of an electrophoresis apparatus and improving the throughput, the present electrophoresis apparatus has two platens capable of controlling temperature of electrophoresis plates placed thereon, a loading medium charging unit for sending a loading medium under pressure, a loading medium charging nozzle mechanism having a pair of nozzles connected to the loading medium charging unit, a pipetter mechanism for dispensing samples to sample dispensing openings of the electrophoresis plates placed on the platens, a stacker mechanism for storing sample plates, a loading buffer solution supplying mechanism, a loading buffer solution dispensing mechanism connected to the loading buffer solution supplying mechanism, a power unit for allowing electrophoresis separation for each electrophoresis plate placed on the platens, and a detector for optically detecting components migrating through each electrophoresis flow channel of the electrophoresis plates.
摘要翻译： 为了使电泳装置的操作自动化并提高通过量，本电泳装置具有能够控制放置在其上的电泳板的温度的两个压板，用于在压力下发送加载介质的装载介质充电单元，具有 连接到装载介质充电单元的一对喷嘴，用于分配样品以对放置在压板上的电泳板的分配开口进行采样的移液器机构，用于存储样品板的堆垛机构，装载缓冲溶液供应机构，装载缓冲溶液 连接到装载缓冲溶液供应机构的分配机构，用于允许放置在台板上的每个电泳板电泳分离的动力单元，以及用于光学检测通过电泳板的每个电泳流路移动的组分的检测器。
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公开(公告)号：US5344613A
公开(公告)日：1994-09-06
申请号：US9080
申请日：1993-01-26
CPC分类号： B01L99/00 , B01J19/0046 , C07K1/045 , B01J2219/00286 , B01J2219/00288 , B01J2219/005 , B01J2219/0059 , B01J2219/00596 , B01J2219/00725 , C40B40/10 , C40B60/14
摘要： In an automated solid-phase peptide synthesizing apparatus comprising at least one reaction vessel containing particulate resin as an insoluble support matrix which anchors elongating peptide chains yielded through coupling-assembly formation of peptide bonds, mixing agitation of reagents, as well as circulation agitation of washing solvents flushing the coupling-associated reaction solutions, introduced in peptide chain-assembly process steps into the reaction vessel, is effected by the bubbling of an inert gas forcibly passed through the reagents or washing solvent via a drainage port of the reaction vessel, in order to promote peptide synthesis. A removable agitation stabilizer is inserted into the reaction vessel as a barrier for inhibiting extra-vessel escape, due to action of the bubbling inert gas, of the reagents as well as the washing solvents, and of the support matrix anchoring the elongating peptide chains. The stabilizer also breaks clumping and coagulative skinning of elongated peptides bound to the support matrix, as effects further consequent of the action of the bubbling inert gas and levitated thereby toward the mouth of the reaction vessel during peptide synthesis. The stabilizer additionally acts as a baffle furthering mixing agitation, to promote dispersive mixing of the peptidyl resin with the reagents and washing solvents during peptide chain assembly; and It also serves to distribute washing solvents evenly along the inner surface of the reaction vessel.
摘要翻译： 在一种自动化固相肽合成装置中，包括至少一个含有颗粒状树脂作为不溶性支持基质的反应容器，该反应容器通过肽键的偶联 - 组装形成固定延伸的肽链，混合搅拌试剂以及循环搅拌洗涤 将通过肽链组装工艺步骤引入反应容器的偶联相关反应溶液冲洗到反应容器中，通过以反应容器的排水口强制通过试剂或洗涤溶剂的惰性气体起泡 促进肽合成。 将可移除的搅拌稳定剂插入到反应容器中，作为阻止由于起泡惰性气体的作用以及洗涤溶剂和固定伸长肽链的载体基质而导致的外部容器逸出的屏障。 稳定剂还破坏结合到载体基质上的细长肽的结块和凝结性皮肤剥离，其作用是由于气泡惰性气体的作用而进一步引起，并且由此在肽合成期间悬浮于反应容器口中。 稳定剂另外作为进一步混合搅拌的挡板，以促进肽链组装期间肽基树脂与试剂和洗涤溶剂的分散混合; 并且还用于沿着反应容器的内表面均匀分布洗涤溶剂。
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公开(公告)号：US20080087547A1
公开(公告)日：2008-04-17
申请号：US11905539
申请日：2007-10-02
申请人： Shin Nakamura , Rintaro Yamamoto , Akira Harada , Makoto Hazama , Takashi Ikegami , Toru Kaji , Naoya Endo , Hidesato Kumagai , Tetsuo Ohashi , Atsushi Inami , Keisuke Miyamoto , Shinichi Utsunomiya
发明人： Shin Nakamura , Rintaro Yamamoto , Akira Harada , Makoto Hazama , Takashi Ikegami , Toru Kaji , Naoya Endo , Hidesato Kumagai , Tetsuo Ohashi , Atsushi Inami , Keisuke Miyamoto , Shinichi Utsunomiya
IPC分类号： G01N27/447 , G01N21/64
CPC分类号： G01N27/44791 , G01N27/44721 , G01N27/44743 , G01N27/44782
摘要： For automating the operation of an electrophoresis apparatus and improving the throughput, the present electrophoresis apparatus has two platens capable of controlling temperature of electrophoresis plates placed thereon, a loading medium charging unit for sending a loading medium under pressure, a loading medium charging nozzle mechanism having a pair of nozzles connected to the loading medium charging unit, a pipetter mechanism for dispensing samples to sample dispensing openings of the electrophoresis plates placed on the platens, a stacker mechanism for storing sample plates, a loading buffer solution supplying mechanism, a loading buffer solution dispensing mechanism connected to the loading buffer solution supplying mechanism, a power unit for allowing electrophoresis separation for each electrophoresis plate placed on the platens, and a detector for optically detecting components migrating through each electrophoresis flow channel of the electrophoresis plates.
摘要翻译： 为了使电泳装置的操作自动化并提高通过量，本电泳装置具有能够控制放置在其上的电泳板的温度的两个压板，用于在压力下发送加载介质的装载介质充电单元，具有 连接到装载介质充电单元的一对喷嘴，用于分配样品以对放置在压板上的电泳板的分配开口进行采样的移液器机构，用于存储样品板的堆垛机构，装载缓冲溶液供应机构，装载缓冲溶液 连接到装载缓冲溶液供应机构的分配机构，用于允许放置在台板上的每个电泳板电泳分离的动力单元，以及用于光学检测通过电泳板的每个电泳流路移动的组分的检测器。
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