公开(公告)号：US20210373000A1

公开(公告)日：2021-12-02

申请号：US17398315

申请日：2021-08-10

Applicant: Element Biosciences, Inc.

Inventor： Sinan ARSLAN ,  Molly HE ,  Matthew KELLINGER ,  Jake LEVIEUX ,  Michael PREVITE ,  Junhua ZHAO ,  Su ZHANG ,  Tyler LOPEZ

IPC: G01N33/53 ,  G01N33/58 ,  C12Q1/6874

Abstract: Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

公开(公告)号：US20210222239A1

公开(公告)日：2021-07-22

申请号：US17016353

申请日：2020-09-09

Applicant: Element Biosciences, Inc.

Inventor： Steve Xiangling CHEN ,  Minghao GUO ,  Michael PREVITE ,  Chunhong ZHOU ,  Derek FULLER

IPC: C12Q1/6869 ,  G01N15/14

Abstract: Methods and systems for sequencing a nucleic acid molecule are described that comprise imaging a first surface and an axially-displaced second surface using a compensation-free optical system, the system comprising an objective lens and at least one image sensor, wherein said optical system has a numerical aperture (NA) of less than 0.6 and a field-of-view (FOV) of greater than 1.0 mm2; and) processing the images of the first surface and the axially-displaced second surface to correct for optical aberration such that the images of the first surface and the axially-displaced second surface have substantially the same optical resolution.

公开(公告)号：US20210123911A1

公开(公告)日：2021-04-29

申请号：US17063608

申请日：2020-10-05

Applicant: Element Biosciences, Inc.

Inventor： Sinan ARSLAN ,  Molly HE ,  Matthew KELLINGER ,  Jake LEVIEUX ,  Michael PREVITE ,  Junhua ZHAO ,  Su ZHANG ,  Tyler LOPEZ

IPC: G01N33/53 ,  C12Q1/6874 ,  G01N33/58

Abstract: Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

公开(公告)号：US20210121882A1

公开(公告)日：2021-04-29

申请号：US17082445

申请日：2020-10-28

Applicant: Element Biosciences, Inc.

Inventor： Minghao GUO ,  Leon Zilun ZHANG ,  Chunhong ZHOU ,  Matthew KELLINGER ,  Michael PREVITE

IPC: B01L3/00

Abstract: Flow cell devices, cartridges, and systems are described that provide reduced manufacturing complexity, lowered consumable costs, and flexible system throughput for nucleic acid sequencing and other chemical or biological analysis applications. The flow cell device can include a capillary flow cell device or a microfluidic flow cell device.

公开(公告)号：US20210040534A1

公开(公告)日：2021-02-11

申请号：US17070796

申请日：2020-10-14

Applicant: ELEMENT BIOSCIENCES, INC.

Inventor： Chunhong ZHOU ,  Sinan ARSLAN ,  Molly Min HE ,  Matthew KELLINGER ,  Adeline Huizhen MAH ,  Michael PREVITE ,  Lei SUN

IPC: C12Q1/682 ,  C12Q1/6869 ,  C12Q1/6867

Abstract: Methods and formulations for preparing low non-specific binding surfaces are described, and the prepared surface can provide improved performance for nucleic acid detection and base calling applications. The surface provides more accurate nucleic acid detection, enhanced contrast to noise ratio, and better data collection.

公开(公告)号：US20200248258A1

公开(公告)日：2020-08-06

申请号：US16855877

申请日：2020-04-22

Applicant: ELEMENT BIOSCIENCES, INC.

Inventor： Sinan ARSLAN ,  Chunhong ZHOU ,  Molly Min HE ,  Matthew KELLINGER ,  Adeline Huizhen MAH ,  Michael PREVITE ,  Lei SUN

IPC: C12Q1/6876 ,  G01N33/543 ,  C12N9/12 ,  G01N21/64

Abstract: Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

公开(公告)号：US20250092455A1

公开(公告)日：2025-03-20

申请号：US18914576

申请日：2024-10-14

Applicant: Element Biosciences, Inc.

Inventor： Arash GHORBANI ,  Russell HUDYMA ,  John BAILEY ,  Michael PREVITE

IPC: C12Q1/6874 ,  C12Q1/6869 ,  G01N21/64

Abstract: Fluorescence imaging system designs are described that provide larger fields-of-view, increased spatial resolution, improved modulation transfer and image quality, higher spatial sampling frequency, faster transitions between image capture when repositioning the fields-of-view, improved imaging system duty cycle and a more compact system, and thus enable higher throughput image acquisition and analysis for genomics and other imaging applications at a lower cost.

公开(公告)号：US20240200133A1

公开(公告)日：2024-06-20

申请号：US18417983

申请日：2024-01-19

Applicant: Element Biosciences, Inc.

Inventor： Arash GHORBANI ,  Russell HUDYMA ,  John BAILEY ,  Michael PREVITE

IPC: C12Q1/6874

CPC classification number: C12Q1/6874

Abstract: Fluorescence imaging system designs are described that provide larger fields-of-view, increased spatial resolution, improved modulation transfer and image quality, higher spatial sampling frequency, faster transitions between image capture when repositioning the fields-of-view, improved imaging system duty cycle and a more compact system, and thus enable higher throughput image acquisition and analysis for genomics and other imaging applications at a lower cost.

公开(公告)号：US20240052398A1

公开(公告)日：2024-02-15

申请号：US18450200

申请日：2023-08-15

Applicant: Element Biosciences, Inc.

Inventor： Michael PREVITE ,  Shawn LEVY

IPC: C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6844

CPC classification number: C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6844

Abstract: The present disclosure provides compositions, apparatuses and methods for capturing on a support nucleic acids from cellular samples, preparing library molecules on the support, amplifying the library molecules on the support to generate nucleic acid template molecules, and analyzing the immobilized nucleic acid template molecules including detecting and/or sequencing the immobilized nucleic acid template molecules. The immobilized nucleic acid template molecules correspond to the nucleic acids from the cellular samples. The immobilized nucleic acid template molecules are spatially located on the support at positions that correspond to the spatial location of the nucleic acids from the cellular sample.

公开(公告)号：US11891651B2

公开(公告)日：2024-02-06

申请号：US17521239

申请日：2021-11-08

Applicant: Element Biosciences, Inc.

Inventor： Sinan Arslan ,  Junhua Zhao ,  Molly He ,  Samantha Snow ,  William Light ,  Matthew Kellinger ,  Michael Previte ,  Michael Kim ,  Hua Yu ,  Yu-Hsien Hwang-Fu ,  Marco Tjioe ,  Andrew Boddicker

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6874 ,  G01N21/64 ,  C12Q1/6853

CPC classification number: C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6853 ,  C12Q1/6874 ,  G01N21/6428 ,  G01N21/6458 ,  C12Q2600/158

Abstract: The present disclosure provides compositions and methods that employ the compositions for conducting pairwise sequencing and for generating concatemer template molecules for pairwise sequencing. The concatemers can be generated using a rolling circle amplification reaction which is conducted either on-support, or conducted in-solution and then distributed onto a support. The rolling circle amplification reaction generates concatemers containing tandem copies of a sequence of interest and at least one universal adaptor sequence. An increase in the number of tandem copies in a given concatemer increases the number of sites along the concatemer for hybridizing to multiple sequencing primers which serve as multiple initiation sites for polymerase-catalyzed sequencing reactions. When the sequencing reaction employs detectably labeled nucleotides and/or detectably labeled multivalent molecules (e.g., having nucleotide units), the signals emitted by the nucleotides or nucleotide units that participate in the parallel sequencing reactions along the concatemer yields an increased signal intensity for each concatemer.