公开(公告)号：US20140235815A1

公开(公告)日：2014-08-21

申请号：US14059285

申请日：2013-10-21

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P7/46 ,  C08G63/00 ,  C07C55/10 ,  C12P7/24 ,  C12N15/70 ,  C12N15/81

CPC classification number: C12P7/46 ,  C07C55/10 ,  C07C59/235 ,  C08G63/00 ,  C12N9/0006 ,  C12N9/0028 ,  C12N9/1007 ,  C12N15/70 ,  C12N15/74 ,  C12N15/77 ,  C12N15/81 ,  C12N15/815 ,  C12P7/24 ,  C12Y101/01244 ,  C12Y105/0102

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as succinate. Also provided herein are methods for using such an organism to produce succinate.

Abstract translation: 本文提供了具有甲醇代谢途径的非天然存在的微生物生物，其可以在甲醇存在下增强还原当量的可用性。 这种还原当量可以用于增加由微生物生物如琥珀酸酯产生的有机化合物的产物产率。 本文还提供了使用这种生物体来生产琥珀酸盐的方法。

公开(公告)号：US11708589B2

公开(公告)日：2023-07-25

申请号：US16894600

申请日：2020-06-05

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Mark J. Burk ,  Robin E. Osterhout ,  Jun Sun ,  Priti Pharkya

IPC: C12P7/18 ,  C12N15/52

CPC classification number: C12P7/18 ,  C12N15/52

Abstract: Provided herein is a non-naturally occurring microbial organism having a 1,3-butanediol (1,3-BDO) pathway and comprising at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. In some embodiments, the pathway includes reducing equivalents from CO or hydrogen. In certain embodiments, a 1,3-BDO pathway proceeds by way of central metabolites pyruvate, succinate or alpha-ketoglutarate. Also provided herein is a method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.

公开(公告)号：US20230136909A1

公开(公告)日：2023-05-04

申请号：US17833695

申请日：2022-06-06

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12N15/52 ,  C12P7/42 ,  C08F120/06 ,  C08G63/664 ,  C12N15/63 ,  C12P7/24 ,  C12P7/40 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12P7/62

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 3-hydroxyisobutyrate or MAA. Also provided herein are methods for using such an organism to produce 3-hydroxyisobutyrate or MAA.

公开(公告)号：US11535874B2

公开(公告)日：2022-12-27

申请号：US14436722

申请日：2013-10-21

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12N9/96 ,  C12P7/46 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C07C55/10 ,  C08G63/00 ,  C12N15/70 ,  C12N15/81 ,  C12P7/24 ,  C07C59/235 ,  C12N15/74 ,  C12N15/77

Abstract: Provided herein is a non-naturally occurring microbial organism (NNOMO) having a methanol metabolic pathway (MMP) that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as succinate. Also provided herein are methods for using such an organism to produce succinate.

公开(公告)号：US20220348932A1

公开(公告)日：2022-11-03

申请号：US17516424

申请日：2021-11-01

Applicant: Genomatica, Inc.

Inventor： Robin E. Osterhout ,  Anthony P. Burgard ,  Priti Pharkya ,  Stefan Andrae

IPC: C12N15/52 ,  C12N9/88 ,  C12N9/90 ,  C12N9/04 ,  C12N9/10 ,  C12P7/04

Abstract: The invention provides non-naturally occurring microbial organisms having a formaldehyde fixation pathway, a formate assimilation pathway, and/or a methanol metabolic pathway in combination with a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway, wherein the microbial organisms selectively produce a fatty alcohol, fatty aldehyde or fatty acid of a specified length or isopropanol. The microbial organisms provided advantageously enhance the production of substrates and/or pathway intermediates for the production of chain length specific fatty alcohols, fatty aldehydes, fatty acids or isopropanol. In some aspects, the microbial organisms of the invention have select gene disruptions or enzyme attenuations that increase production of fatty alcohols, fatty aldehydes or fatty acids. The invention additionally provides methods of using the above microbial organisms to produce a fatty alcohol, a fatty aldehyde, a fatty acid or isopropanol.

公开(公告)号：US11447804B2

公开(公告)日：2022-09-20

申请号：US16178432

申请日：2018-11-01

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P17/10 ,  C12P7/44 ,  C12P7/24 ,  C12P13/00 ,  C12N9/04 ,  C08G63/78 ,  C08G73/02 ,  C08G69/16 ,  C08G69/08 ,  A23L29/00 ,  C12N15/52

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as adipate, 6-aminocaproate, hexamethylenediamine or caprolactam. Also provided herein are methods for using such an organism to produce adipate, 6-aminocaproate, hexamethylenediamine or caprolactam.

公开(公告)号：US11384340B2

公开(公告)日：2022-07-12

申请号：US16792592

申请日：2020-02-17

Applicant: Genomatica, Inc.

Inventor： Stefan Andrae ,  Michael Patrick Kuchinskas ,  Jingyi Li ,  Harish Nagarajan ,  Priti Pharkya

IPC: C12N9/04 ,  C12P7/24 ,  C12P7/18

Abstract: Described herein are non-natural NAD+-dependent alcohol dehydrogenases (ADHs) capable of at least two fold greater conversion of methanol or ethanol to formaldehyde or acetaldehyde, respectively, as compared to its unmodified counterpart. Nucleic acids encoding the non-natural alcohol dehydrogenases, as well as expression constructs including the nucleic acids, and engineered cells comprising the nucleic acids or expression constructs are described. Also described are engineered cells expressing a non-natural NAD+-dependent alcohol dehydrogenase, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.

公开(公告)号：US20210238609A1

公开(公告)日：2021-08-05

申请号：US17008243

申请日：2020-08-31

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Priti Pharkya ,  Stefan Andrae ,  Ewa Teresa Lis ,  Carla Risso ,  John Douglas Trawick

IPC: C12N15/63 ,  C12P5/02 ,  C12P7/04 ,  C12P7/18 ,  C12N9/88 ,  C07K14/195 ,  C12N15/52 ,  C12N9/10 ,  C12N9/90 ,  C07C1/24 ,  C07C11/167 ,  C07C31/20 ,  C07C33/025 ,  C09D147/00 ,  C09J147/00

Abstract: Provided herein are non-naturally occurring microbial organisms having a FaldFP, a FAP and/or metabolic modifications which can further include a MMP, a MOP, a hydrogenase and/or a CODH. These microbial organisms can further include a butadiene, 13BDO, CrotOH, MVC or 3-buten-1-ol pathway. Additionally provided are methods of using such microbial organisms to produce butadiene, 13BDO, CrotOH, MVC or 3-buten-1-ol.

公开(公告)号：US10669561B2

公开(公告)日：2020-06-02

申请号：US15323360

申请日：2015-07-02

Applicant: Genomatica, Inc.

Inventor： Stephanie J. Culler ,  Mark J. Burk ,  Robin E. Osterhout ,  Priti Pharkya

IPC: C12N15/00 ,  C12N1/20 ,  C12N9/00 ,  C12N9/02 ,  C12N9/88 ,  C12N9/90 ,  C07H21/04 ,  C12P7/04 ,  C12N15/52 ,  C12P5/02 ,  C12P7/40 ,  C12N9/04

Abstract: The invention provides a non-naturally occurring microbial organism having a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol, pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a pathway. The invention additionally provides a method for producing butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol. The method can include culturing a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol-producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a pathway enzyme in a sufficient amount, and under conditions and for a sufficient period of time to produce butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol.

公开(公告)号：US20190300919A1

公开(公告)日：2019-10-03

申请号：US16178432

申请日：2018-11-01

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P17/10 ,  C12P13/00 ,  A23L29/00 ,  C12N15/52 ,  C08G69/08 ,  C08G69/16 ,  C12P7/24 ,  C08G63/78 ,  C12P7/44 ,  C08G73/02 ,  C12N9/04

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as adipate, 6-aminocaproate, hexamethylenediamine or caprolactam. Also provided herein are methods for using such an organism to produce adipate, 6-aminocaproate, hexamethylenediamine or caprolactam.