Shuttle vector comprising a T-DNA Region and RI and RK2 origins of
replication
    91.
    发明授权
    Shuttle vector comprising a T-DNA Region and RI and RK2 origins of replication 失效
    穿梭载体包含T-DNA区和RI和RK2复制起点

    公开(公告)号:US6165780A

    公开(公告)日:2000-12-26

    申请号:US937902

    申请日:1997-09-25

    申请人: Shinji Kawasaki

    发明人: Shinji Kawasaki

    摘要: The invention provides a high capacity binary shuttle vector having T-DNA region and Ri ori and capable to integrate a large genome fragment in it; a genomic library having the ability to transform a plant, especially monocotyledonous ones; a plant transformed with the high capacity binary shuttle vector; and a method of searching for a useful gene by use of the above vector. The present high capacity binary shuttle vector can introduce a large genome fragment of 10 kb or more, easily, efficiently and stably into a plant, especially monocotyledonous ones under those conditions in which the rearrangement or deletion of these genome fragments does not occur. The invention also provides some other vectors derived from the above Ri ori driven vectors, which can integrate circular genome library plasmids with a lox site, or which can efficiently transform plants with genes of arbitrary expression properties. The invention includes also the assessment method for the functions of genome fragments inserted in the above vectors by complementation assay. In addition, a useful gene or gene complex can be efficiently examined so its isolation is also made easy.

    摘要翻译: 本发明提供了具有T-DNA区和Ri ori并能够整合其中的大基因组片段的高容量二元穿梭载体; 具有转化植物,特别是单子叶植物的能力的基因组文库; 用高容量二元穿梭载体转化的植物; 以及通过使用上述载体搜索有用基因的方法。 目前的高容量二元穿梭载体可以在不发生这些基因组片段的重排或缺失的条件下,将10kb或更大的10kb或更大的基因组片段引入植物,特别是单子叶植物中。 本发明还提供衍生自上述Ri ori驱动载体的一些其它载体,其可以将圆形基因组文库质粒与lox位点整合,或可以用任意表达性质的基因有效转化植物。 本发明还包括通过互补测定插入上述载体中的基因组片段的功能的评估方法。 另外,可以有效地检查有用的基因或基因复合体,使其分离也容易。

    Facsimile machine having computer-linked mode
    92.
    发明授权
    Facsimile machine having computer-linked mode 失效
    具有计算机连接模式的传真机

    公开(公告)号:US6144463A

    公开(公告)日:2000-11-07

    申请号:US668716

    申请日:1996-06-24

    IPC分类号: H04N1/00

    摘要: A facsimile machine is connected to another facsimile machine by a telephone line and to a computer by an exclusive line. The facsimile machine has a computer-linked mode and an ordinary facsimile mode. If the facsimile machine is in the computer-linked mode, the image data transmission between the facsimile machine and the computer is performed based on an instruction command from an operator of the computer. If the facsimile machine is in the ordinary facsimile mode, the image data transmission between the facsimile machine and another facsimile machine is performed in an ordinary facsimile transmission manner. When an instruction command comes from the computer, the facsimile machine sends a response signal (or message) to the computer in answer to the instruction command. Thus, the facsimile machine operates under the supervision of the computer. This improves the control reliability.

    摘要翻译: 传真机通过电话线连接到另一传真机,并通过专用线连接到计算机。 传真机具有计算机连接模式和普通的传真模式。 如果传真机处于计算机连接模式,则基于来自计算机的操作者的指令命令来执行传真机和计算机之间的图像数据传输。 如果传真机处于普通的传真模式,传真机和其他传真机之间的图像数据传输是以普通的传真方式进行的。 当来自计算机的指令命令时,传真机根据指令命令向计算机发送响应信号(或消息)。 因此,传真机在计算机的监督下运行。 这提高了控制的可靠性。

    Method for transmitting data with tolerance for superimposed data
    93.
    发明授权
    Method for transmitting data with tolerance for superimposed data 失效
    用于传输数据的方法用于叠加数据

    公开(公告)号:US6134248A

    公开(公告)日:2000-10-17

    申请号:US933741

    申请日:1997-09-23

    CPC分类号: G08C15/00 G08C25/00

    摘要: A method for transmitting data from each of n transmitting devices (1) to (n) is provided. According to this method, the data is transmitted with each of a plurality of transmission cycles being one group. Each of the plurality of transmission cycles includes a transmission unit and a first transmission rest period, and the transmission unit includes at least n transmission periods and at least (n-1) second transmission rest periods. The transmission periods and the second transmission rest periods are alternately repeated. The method includes the step of transmitting send data and determination data for determining whether or not two given transmission periods belong to an identical transmission cycle during each of the at least n transmission periods, where n is a positive integer.

    摘要翻译: 提供了一种从n个发送装置(1)到(n)中的每一个发送数据的方法。 根据该方法,以多个传输周期中的每一个为一组发送数据。 多个发送周期中的每一个包括发送单元和第一发送休止期间,并且发送单元至少包括n个发送周期和至少(n-1)个第二发送休止期间。 交替地重复发送周期和第二发送休止期间。 该方法包括以下步骤:发送数据和确定数据,以确定在至少n个传输周期中的每一个期间两个给定的传输周期是否属于相同的传输周期,其中n是正整数。

    Method for corrugating a metallic pipe
    95.
    发明授权
    Method for corrugating a metallic pipe 失效
    瓦楞金属管的方法

    公开(公告)号:US5711177A

    公开(公告)日:1998-01-27

    申请号:US671180

    申请日:1996-06-27

    IPC分类号: B21D17/02 B21D26/02 B21D31/04

    CPC分类号: B21D17/025

    摘要: A method of corrugating a metallic pipe which achieves an increase in the limit of tube expansion ratios. First, a heated part is formed by heating a local part of an outer periphery of a pipe in a circumferential direction by a high frequency coil. Next, the heated part is located within a forming surface of a forming die, and an axial compressive stress is applied to the pipe. Thus, the heated part is expanded while restricted by the forming surface. Therefore, even when the axial length of the heated part is increased, formability is stabilized and the tube expansion ratio can be raised.

    摘要翻译: 一种使金属管波纹化的方法,其实现了管膨胀比的极限的增加。 首先,通过高频线圈在周向上加热管的外周的局部部分来形成加热部。 接下来,加热部位于成形模具的成形面内,向管道施加轴向压缩应力。 因此,加热部分被形成表面限制地扩张。 因此,即使加热部件的轴向长度增加,成型性也稳定,管膨胀率提高。

    Nucleic acid markers for rice blast resistance genes and rice blast
resistance genes isolated by the use of these markers
    96.
    发明授权
    Nucleic acid markers for rice blast resistance genes and rice blast resistance genes isolated by the use of these markers 失效
    通过使用这些标记分离的稻瘟病抗性基因和稻瘟病抗性基因的核酸标记

    公开(公告)号:US5674993A

    公开(公告)日:1997-10-07

    申请号:US282556

    申请日:1994-07-29

    摘要: The present invention provides nucleic acid markers for rice blast resistance genes, which are isolated from the rice genomic DNA, are DNA sequences having a total length of up to 2 kb, with two identical sequences of at least ten bases at the both ends thereof and are located within a distance of 2.0 cM from rice blast resistance genes Pi-b, Pi-ta or Pi-ta.sup.2 . According to the present invention, it becomes possible to easily isolate rice blast resistance genes and related genes from rice cultivars containing Pi-b, Pi-ta.sup.2 or Pi-ta, thus promoting development and breeding of superior cultivars. It becomes also possible to easily carry out a resistance test of rice. This will open up the way to create new resistance genes.

    摘要翻译: 本发明提供了从水稻基因组DNA中分离的稻瘟病抗性基因的核酸标记,其总长度高达2kb的DNA序列,两端具有至少十个碱基的两个相同序列, 位于距离稻瘟病基因+ E,不是Pi-b + EE,+ E,uns Pi-ta + EE或+ E,uns Pi-ta2 + EE的2.0 cM的距离内。 根据本发明,可以容易地从含有+ E,不是Pi-b + EE,+ E,不是Pi-ta2 + EE或+ E的水稻品种中分离稻瘟病抗性基因和相关基因, + EE,从而促进优良品种的发育和育种。 也可以容易地进行大米的电阻试验。 这将打开创造新的抗性基因的方式。

    Solid oxide fuel cells, a process for producing solid electrolyte films
and a process for producing solid oxide fuel cells
    97.
    发明授权
    Solid oxide fuel cells, a process for producing solid electrolyte films and a process for producing solid oxide fuel cells 失效
    固体氧化物燃料电池,固体电解质膜的制造方法以及固体氧化物燃料电池的制造方法

    公开(公告)号:US5527633A

    公开(公告)日:1996-06-18

    申请号:US119690

    申请日:1993-09-13

    摘要: A solid oxide fuel cell having an ion conductive solid electrolyte layer formed by a spraying method, wherein a thickness of the solid electrolyte layer is not less then 40 .mu.m and not more than 100 .mu.m, and a leakage amount of N.sub.2 gas of the solid electrolyte layer at room temperature is not more than 10.sup.-5 cc/g.second. A permeation coefficient of the solid electrolyte layer is preferably not more than 10.sup.-7 cm.sup.4 /g.second at room temperature. The solid electrolyte layer includes at least one metal element selected from manganese, iron, cobalt, nickel, copper and zinc, in an average amount of not less than 1 atom % and not more than 15 atom % based on a sum of amounts of all metal elements contained in the solid electrolyte layer.

    摘要翻译: 一种具有通过喷雾法形成的离子传导性固体电解质层的固体氧化物型燃料电池,其特征在于,固体电解质层的厚度为40μm以上且100μm以下,并且, 固体电解质层在室温下不超过10-5 cc / g。 固体电解质层的透过系数在室温下优选为10-7cm4 / g以下。 所述固体电解质层包含选自锰,铁,钴,镍,铜,锌中的至少一种金属元素,平均含量为1原子%以上15原子%以下 包含在固体电解质层中的金属元素。

    Method for manufacturing solid oxide film and method for manufacturing
solid oxide fuel cell using the solid oxide film
    100.
    发明授权
    Method for manufacturing solid oxide film and method for manufacturing solid oxide fuel cell using the solid oxide film 失效
    固体氧化物膜的制造方法以及使用固体氧化物膜制造固体氧化物型燃料电池的方法

    公开(公告)号:US5358735A

    公开(公告)日:1994-10-25

    申请号:US857965

    申请日:1992-03-26

    摘要: A first method for manufacturing a solid oxide film having a highly densified solid oxide film having a small thickness and an improved electric conductivity; and a second method for manufacturing a solid oxide fuel cell in which a solid oxide film formed on an air electrode or a fuel electrode is manufactured by the first method. The solid oxide fuel cell according to the present invention is capable of generating a high power. The solid oxide film is formed on a substrate in such manner that a solid oxide material is sprayed on the substrate to form a sprayed solid oxide film thereon; Then a solution of metal compound including at least one metal selected from a group of manganese, iron, cobalt, nickel, copper and zinc is impregnated into the sprayed solid oxide film; and the sprayed solid oxide film is subjected to a heat treatment in order to increase an airtightness of the film. It may be possible to use a material for spraying, in which 1.about.10 parts by weight of an oxide of said metal is contained, instead of the impregnation, or to obtain a material for spraying by mixing the compound powder containing the metal and the solid oxide material in a spray gun via separately arranged powder supply devices.

    摘要翻译: 制造具有厚度小且电导率提高的高致密固体氧化物膜的固体氧化物膜的第一种方法; 以及通过第一种方法制造其中形成在空气电极或燃料电极上的固体氧化物膜的固体氧化物燃料电池的第二种方法。 根据本发明的固体氧化物型燃料电池能够产生高功率。 固体氧化物膜以这样的方式形成在基板上,即将固体氧化物材料喷涂在基板上以在其上形成喷射的固体氧化物膜; 然后将包含选自锰,铁,钴,镍,铜和锌中的至少一种金属的金属化合物溶液浸渍到喷涂的固体氧化物膜中; 并且对喷射的固体氧化物膜进行热处理以增加膜的气密性。 可以使用其中含有10重量份所述金属的氧化物而不是浸渍的喷涂材料,或者通过混合含有金属的化合物粉末和 通过单独布置的粉末供应装置在喷枪中固体氧化物材料。