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256 条记录 (耗时 0.043 秒)

    Amylase variants
    103.
    发明授权
    Amylase variants 失效
    淀粉酶变体

    公开(公告)号：US6093562A

    公开(公告)日：2000-07-25

    申请号：US600656

    申请日：1996-02-13

    申请人： Henrik Bisg.ang.rd-Frantzen ,  Allan Svendsen ,  Torben Vedel Borchert

    发明人： Henrik Bisg.ang.rd-Frantzen ,  Allan Svendsen ,  Torben Vedel Borchert

    IPC分类号： C11D3/386 ,  C12N9/28 ,  C12N15/56 ,  D06L1/14

    CPC分类号： C11D3/38618 ,  C11D3/38609 ,  C11D3/38681 ,  C12N9/2417 ,  C12Y302/01001 ,  D06L1/14

    摘要： The present invention relates to variants of a parent .alpha.-amylase, which parent .alpha.-amylase (i) has an amino acid sequence selected from the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, and SEQ ID No. 7, respectively; or (ii) displays at least 80% homology with one or more of these amino acid sequences; and/or displays immunological cross-reactivity with an antibody raised against an .alpha.-amylase having one of these amino acid sequences; and/or is encoded by a DNA sequence which hybridizes with the same probe as a DNA sequence encoding an .alpha.-amylase having one of these amino acid sequences; in which variant:(a) at least one amino acid residue of the parent .alpha.-amylase has been deleted; and/or(b) at least one amino acid residue of the parent .alpha.-amylase has been replaced by a different amino acid residue; and/or(c) at least one amino acid residue has been inserted relative to the parent .alpha.-amylase; the variant having .alpha.-amylase activity and exhibiting at least one of the following properties relative to the parent .alpha.-amylase: increased thermostability; increased stability towards oxidation; and reduced Ca.sup.2+ dependency;with the proviso that the amino acid sequence of the variant is not identical to any of the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3 and SEQ ID No. 7, respectively.

    摘要翻译： 本发明涉及母体α-淀粉酶的变体，其母体α-淀粉酶（i）具有选自SEQ ID No.1，SEQ ID No.2，SEQ ID No.2所示的氨基酸序列的氨基酸序列。 3和SEQ ID No.7; 或（ii）与这些氨基酸序列中的一个或多个显示至少80％的同源性; 和/或显示与针对具有这些氨基酸序列之一的α-淀粉酶产生的抗体的免疫交叉反应性; 和/或由与编码具有这些氨基酸序列之一的α-淀粉酶的DNA序列相同的探针杂交的DNA序列编码; 在该变体中：（a）母体α-淀粉酶的至少一个氨基酸残基已被缺失; 和/或（b）母体α-淀粉酶的至少一个氨基酸残基被不同的氨基酸残基替代; 和/或（c）相对于母体α-淀粉酶插入至少一个氨基酸残基; 所述变体具有α-淀粉酶活性并且相对于母体α-淀粉酶显示出至少一种以下性质：增加的热稳定性; 提高氧化稳定性; 并减少Ca2 +依赖; 条件是变体的氨基酸序列分别与SEQ ID No.1，SEQ ID No.2，SEQ ID No.3和SEQ ID No.7所示的任何氨基酸序列不同。

    Amylase variants
    105.
    发明授权
    Amylase variants 失效
    淀粉酶变体

    公开(公告)号：US5830837A

    公开(公告)日：1998-11-03

    申请号：US343804

    申请日：1994-11-22

    申请人： Henrik Bisg.ang.rd-Frantzen ,  Torben Vedel Borchert ,  Allan Svendsen ,  Marianne Thellersen ,  Pia Van der Zee

    发明人： Henrik Bisg.ang.rd-Frantzen ,  Torben Vedel Borchert ,  Allan Svendsen ,  Marianne Thellersen ,  Pia Van der Zee

    IPC分类号： C11D3/386 ,  C12N9/28

    CPC分类号： C11D3/386

    摘要： A variant of a parent .alpha.-amylase enzyme having an improved washing and/or dishwashing performance as compared to the parent enzyme, wherein one or more amino acid residues of the parent enzyme have been replaced by a different amino acid residue and/or wherein one or more amino acid residues of the parent .alpha.-amylase have been deleted and/or wherein one or more amino acid residues have been added to the parent .alpha.-amylase enzyme, provided that the variant is different from one in which the methionine residue in position 197 of a parent B. licheniformis .alpha.-amylase has been replaced by alanine or threonine, as the only modification being made. The variant may be used for washing and dishwashing.

    摘要翻译： 与母体酶相比，具有改善的洗涤和/或餐具洗涤性能的母体α-淀粉酶的变体，其中母体酶的一个或多个氨基酸残基已被不同的氨基酸残基替代和/或其中一个 或更多的母体α-淀粉酶的氨基酸残基已经缺失和/或其中一个或多个氨基酸残基已加入到母体α-淀粉酶中，条件是该变体不同于其中位置上的甲硫氨酸残基的变体 作为唯一的修改，母本地衣芽孢杆菌α-淀粉酶的197已被丙氨酸或苏氨酸代替。 该变体可用于洗涤和洗碗。

    Insulin precursors
    107.
    发明授权
    Insulin precursors 失效
    胰岛素前列腺素

    公开(公告)号：US5202415A

    公开(公告)日：1993-04-13

    申请号：US623739

    申请日：1990-12-13

    申请人： Ib Jonassen ,  Ib G. Clausen ,  Einer B. Jensen ,  Allan Svendsen

    发明人： Ib Jonassen ,  Ib G. Clausen ,  Einer B. Jensen ,  Allan Svendsen

    IPC分类号： C12N15/09 ,  A61K38/00 ,  C07K14/575 ,  C07K14/62 ,  C12N15/17 ,  C12P21/02 ,  C12R1/865

    CPC分类号： C07K14/62 ,  A61K38/00

    摘要： Insulin precursors characterized by the amino acid sequence B(1-29)-X.sub.1 -X.sub.2 -Y.sub.2 -Y.sub.1 -A(1-21), wherein B(1-29) are the 29 first amino acid residues of the B chain of human insulin starting from the N-terminus, A(1-21) are the 21 amino acid residues of the A chain of human insulin, X.sub.1 represents a peptide bond or one or more arbitrary amino acid residues, X.sub.2 represents Glu or Asp, and Y.sub.1 and Y.sub.2 each represents Lys or Arg, the positions A6 and A11, A7 and B7 and A20 and B19, respectively, are connected through sulphur bridges, and, if desired, one or more of the amino acid residues of the chains B(1-29) and A(1-21) are substituted by another amino acid residue, are provided. The insulin precursors are prepared by culturing a yeast strain transformed with a replicable expression vehicle comprising a DNA sequence encoding an insulin precursor of the above formula in a suitable medium and isolating the insulin precursor thus formed from the culture medium. The insulin precursors can be converted into human insulin or insulin analogues by enzymatic treatment in a manner known per se.