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公开(公告)号：US5869438A
公开(公告)日：1999-02-09
申请号：US479275
申请日：1995-06-07
申请人： Allan Svendsen , Shamkant Anant Patkar , Erik Gormsen , Jens Sigurd Okkels , Marianne Thellersen
发明人： Allan Svendsen , Shamkant Anant Patkar , Erik Gormsen , Jens Sigurd Okkels , Marianne Thellersen
CPC分类号： C12N9/20 , C11D3/38627 , C11D3/38663 , C11D3/38672 , C07K2319/04
摘要： The present invention relates to lipase variants which exhibit improved properties, detergent compositions comprising said lipase variants, DNA constructs coding for said lipase variants, and methods of making said lipase variants.
摘要翻译： 本发明涉及表现出改善的性质的脂肪酶变体，包含所述脂肪酶变体的洗涤剂组合物，编码所述脂肪酶变体的DNA构建体，以及制备所述脂肪酶变体的方法。
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公开(公告)号：US5892013A
公开(公告)日：1999-04-06
申请号：US488271
申请日：1995-09-05
申请人： Allan Svendsen , Shamkant Anant Patkar , Erik Gormsen , Ib Groth Clausen , Jens Sigurd Okkels , Marianne Thellersen
发明人： Allan Svendsen , Shamkant Anant Patkar , Erik Gormsen , Ib Groth Clausen , Jens Sigurd Okkels , Marianne Thellersen
CPC分类号： C11D3/38627 , C11D3/38663 , C11D3/38672 , C12N9/20 , C07K2319/04
摘要： The present invention relates to lipase variants which exhibit improved properties, detergent compositions comprising said lipase variants, DNA constructs coding for said lipase variants, and methods of making said lipase variants.
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公开(公告)号：US07157262B2
公开(公告)日：2007-01-02
申请号：US10232544
申请日：2002-08-30
申请人： Claus Crone Fuglsang , Jens Sigurd Okkels , Dorte Aaby Petersen , Shamkant Anant Patkar , Marianne Thellersen , Allan Svendsen , Kim Borch , John C. Royer , Titus Kretzschmar , Torben Halkier , Jesper Vind , Steen Troels Jorgensen
发明人： Claus Crone Fuglsang , Jens Sigurd Okkels , Dorte Aaby Petersen , Shamkant Anant Patkar , Marianne Thellersen , Allan Svendsen , Kim Borch , John C. Royer , Titus Kretzschmar , Torben Halkier , Jesper Vind , Steen Troels Jorgensen
CPC分类号： C11D3/38627 , C12N9/20
摘要： The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzyme capable of removing a substantial amount of fatty matter during a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.
摘要翻译： 本发明涉及具有脂肪分解活性的修饰酶，能够在一次循环洗涤期间除去大量脂肪物质的脂肪分解酶，编码所述酶的DNA序列，包含所述DNA序列的载体，携带所述DNA的宿主细胞 序列或所述载体，以及用于产生具有脂肪分解活性的所述酶的方法。
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公开(公告)号：US06495357B1
公开(公告)日：2002-12-17
申请号：US09007288
申请日：1998-01-14
申请人： Claus Crone Fuglsang , Jens Sigurd Okkels , Dorte Aaby Petersen , Shamkant Anant Patkar , Marianne Thellersen , Allan Svendsen , Kim Borch , John C. Royer , Titus Kretzschmar , Torben Halkier , Jesper Vind , Steen Troels Jorgensen
发明人： Claus Crone Fuglsang , Jens Sigurd Okkels , Dorte Aaby Petersen , Shamkant Anant Patkar , Marianne Thellersen , Allan Svendsen , Kim Borch , John C. Royer , Titus Kretzschmar , Torben Halkier , Jesper Vind , Steen Troels Jorgensen
IPC分类号： C12N920
CPC分类号： C11D3/38627 , C12N9/20
摘要： The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzime capable of removing a substantial amount of fatty matter a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.
摘要翻译： 本发明涉及一种具有脂肪分解活性的修饰酶，能够一周期洗涤大量脂肪物质的脂肪分解酶，编码所述酶的DNA序列，包含所述DNA序列的载体，携带所述DNA序列的宿主细胞 或所述载体，以及用于产生具有脂肪分解活性的所述酶的方法。
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公开(公告)号：US5976855A
公开(公告)日：1999-11-02
申请号：US701339
申请日：1996-08-22
IPC分类号： C12N15/09 , C11D3/386 , C11D7/42 , C12N1/19 , C12N1/21 , C12N9/20 , C12N15/55 , C12R1/69 , C07H21/04 , C12N1/00 , C12N1/20
CPC分类号： C12N9/20 , C11D3/38627 , C11D3/38663 , Y10S435/832 , Y10S435/849 , Y10S435/874 , Y10S435/886 , Y10S435/911 , Y10S435/913
摘要： The present invention relates to a method of preparing a variant of a parent lipolytic enzyme, comprising (a) subjecting a DNA sequence encoding the parent lipolytic enzyme to random mutagenesis, (b) expressing the mutated DNA sequence obtained in step (a) in a host cell, and (c) screening for host cells expressing a mutated lipolytic enzyme which has a decreased dependance to calcium and/or an improved tolerance towards a detergent or a detergent component as compared to the parent lipolytic enzyme.
摘要翻译： 本发明涉及一种制备母体脂肪分解酶的变体的方法，其包括（a）使编码亲本脂肪分解酶的DNA序列进行随机诱变，（b）将步骤（a）中获得的突变的DNA序列表达于 宿主细胞，和（c）与母体脂肪分解酶相比，筛选表达与钙相关性降低和/或改善的对洗涤剂或洗涤剂组分的耐受性的突变的脂肪分解酶的宿主细胞。
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6.发明授权Lipolytic enzyme variant with improved stability and polynucleotides encoding same 有权具有改善稳定性的脂肪分解酶变体和编码相同的多核苷酸公开(公告)号：US09163203B2
公开(公告)日：2015-10-20
申请号：US12393512
申请日：2009-02-26
申请人： Jesper Vind , Kim Borch , Allan Svendsen , Robert Van der Lans , Lise Munch Mikkelsen , Christian Isak Jorgensen , Shamkant Anant Patkar
发明人： Jesper Vind , Kim Borch , Allan Svendsen , Robert Van der Lans , Lise Munch Mikkelsen , Christian Isak Jorgensen , Shamkant Anant Patkar
CPC分类号： C11D3/38627 , C12N9/20 , C12N15/00
摘要： The invention provides lipolytic enzyme variants having improved in-detergent stability and polynucleotides encoding same. Lipolytic enzyme variants with improved in-detergent stability are obtained by substituting certain specified amino acid residues in a parent lipolytic enzyme.
摘要翻译： 本发明提供具有改进的洗涤剂稳定性的脂肪分解酶变体和编码它的多核苷酸。 通过用母体脂肪分解酶中的某些特定的氨基酸残基取代具有改善的洗涤剂稳定性的脂肪分解酶变体。
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公开(公告)号：US07396657B2
公开(公告)日：2008-07-08
申请号：US11602553
申请日：2006-11-21
申请人： Signe Munk , Jesper Vind , Kim Borch , Shamkant Anant Patkar , Sanne O. Schroder Glad , Allan Svendsen
发明人： Signe Munk , Jesper Vind , Kim Borch , Shamkant Anant Patkar , Sanne O. Schroder Glad , Allan Svendsen
CPC分类号： C11D3/38627
摘要： Attaching a peptide extension to the C-terminal amino acid of a lipase reduces the tendency to form odor. This may lead to lipase variants with a reduced odor generation when washing textile soiled with fat which includes relatively short-chain fatty acyl groups (e.g., up to C8) such as dairy stains containing butter fat or tropical oils such as coconut oil or palm kernel oil.
摘要翻译： 将肽延伸连接到脂肪酶的C末端氨基酸可降低形成气味的倾向。 当洗涤用脂肪污染的纺织品时，可能导致脂肪酶变体具有减少的气味产生，所述脂肪包括相对短链的脂肪酰基（例如高达C 8 N 8），例如含有脂肪或热带 油如椰子油或棕榈仁油。
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公开(公告)号：US08679774B2
公开(公告)日：2014-03-25
申请号：US12849911
申请日：2010-08-04
申请人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
发明人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
CPC分类号： C12Y301/01003 , A21D8/042 , C11B3/003 , C11D3/38627 , C12N9/20 , C12P9/00
摘要： The substrate specificity of a lipolytic enzyme can be modified by making alterations to the amino acid sequence in a defined region of the lipolytic enzyme, so as to increase the level of a desired activity or to decrease the level of an undesired activity. Thus, the inventors have developed lipolytic enzyme variants with a modified amino acid sequence with a substrate specificity which can be tailored for specific uses.
摘要翻译： 可以通过改变脂肪分解酶的限定区域中的氨基酸序列来修饰脂解酶的底物特异性，从而增加所需活性的水平或降低不需要的活性的水平。 因此，本发明人已经开发了具有修饰的氨基酸序列的脂肪分解酶变体，其具有底物特异性，其可以针对特定用途进行定制。
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公开(公告)号：US20100105092A1
公开(公告)日：2010-04-29
申请号：US12443625
申请日：2007-10-02
申请人： Allan Svendsen , Jesper Vind , Shamkant Anant Patkar , Kim Borch
发明人： Allan Svendsen , Jesper Vind , Shamkant Anant Patkar , Kim Borch
CPC分类号： C12N9/20 , C07K2299/00
摘要： Variants with increased acyl transferase activity can be designed on the basis of a three-dimensional model by making amino acid alterations near the active Ser of lipolytic enzymes such as C. antarctica lipase A or the lipase/acyl transferase from C. parapsilosis.
摘要翻译： 具有增加的酰基转移酶活性的变体可以在三维模型的基础上通过在脂肪分解酶如南极洲洲油脂酶A或来自副杆菌病的脂肪酶/酰基转移酶的活性Ser附近进行氨基酸改变来设计。
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公开(公告)号：US20100047836A1
公开(公告)日：2010-02-25
申请号：US12515686
申请日：2007-11-26
申请人： Leonardo De Maria , Jesper Brask , Michael Skjot , Shamkant Anant Patkar , Kim Borch , Allan Svendsen
发明人： Leonardo De Maria , Jesper Brask , Michael Skjot , Shamkant Anant Patkar , Kim Borch , Allan Svendsen
CPC分类号： C12N9/20 , C12P7/40 , C12P7/62 , C12P7/6418 , C12Y301/01003
摘要： Molecular dynamics (MD) simulation on the three-dimensional structure of Candida anrtarctica lipase B revealed two hitherto unknown lids with a marked mobility, and this discovery was used to design lipolytic enzyme variants with increased lipolytic enzyme activity.
摘要翻译： 针对念珠菌脂肪酶B的三维结构的分子动力学（MD）模拟显示两个具有明显迁移率的未知盖，并且该发现用于设计具有增加的脂肪分解酶活性的脂肪分解酶变体。
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