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    GENOME ENGINEERING USING CRISPR RNA-GUIDED INTEGRASES
    112.
    发明公开
    GENOME ENGINEERING USING CRISPR RNA-GUIDED INTEGRASES 审中-公开

    公开(公告)号：US20230147495A1

    公开(公告)日：2023-05-11

    申请号：US17907510

    申请日：2021-03-26

    Applicant: Phuc Hong VO ,  THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

    Inventor： Phuc Hong Vo ,  Samuel Sternberg

    IPC: C12N15/90 ,  C12N15/10 ,  C12N15/113 ,  C12N9/22

    CPC classification number: C12N15/902 ,  C12N15/102 ,  C12N15/113 ,  C12N9/22 ,  C12N2310/20

    Abstract: The present disclosure provides systems and methods for targeted nucleic acid deletions and inactivation of a gene of interest comprising a Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR associated (Cas) (CRISPR-Cas) system comprising at least one Cas protein and a pair of guide RNAs (gRNAs), an engineered transposon system, at least one donor nucleic acid, and a recombinase. The present disclosure also provides methods for genetically modifying diverse bacterial communities comprising contacting a recipient bacterial community with donor bacteria, the donor bacteria comprising a vector encoding: an engineered CRISPR-Cas system, wherein the engineered CRISPR-Cas system comprises: at least one Cas protein and at least one guide RNA (gRNA); an engineered transposon system; at least one donor nucleic acid to be integrated comprising at least one transposon end sequence, and, optionally a recombinase, wherein the donor nucleic acid further comprises a recognitions site for the recombinase.

    DEVICES, COMPOSITIONS AND METHODS FOR IMAGING WITH RAMAN SCATTERING
    115.
    发明申请
    DEVICES, COMPOSITIONS AND METHODS FOR IMAGING WITH RAMAN SCATTERING 有权

    公开(公告)号：US20230128716A1

    公开(公告)日：2023-04-27

    申请号：US17882938

    申请日：2022-08-08

    Applicant: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

    Inventor： WEI MIN ,  LU WEI ,  ZHIXING CHEN ,  FANGHAO HU ,  YIHUI SHEN

    IPC: G01N33/58 ,  G01N33/50 ,  A61K49/00

    Abstract: Methods, systems and computer-accessible medium for imaging a living cell or a living organism with bond-edited compounds using stimulated Raman scattering are disclosed. The method comprises the steps of introducing one or more bond-edited compounds into a live cell or a living organism, and detecting a vibrational tag in the cell or organism with stimulated Raman scattering. Also disclosed are methods for detecting a disease condition in a subject, methods for monitoring treatment for a disease condition, methods for screening an agent, methods for tracing a cellular process in a live cell using bond-edited compounds in combination with stimulated Raman scattering. Also disclosed are a composition for labeling a target cell with at least one bond-edited compound and devices for imaging bond-edited compounds by stimulated Raman scattering.

    Next-generation biomarkers to detect sun damage and predict skin cancer risk
    120.
    发明授权
    Next-generation biomarkers to detect sun damage and predict skin cancer risk 有权

    公开(公告)号：US11613784B2

    公开(公告)日：2023-03-28

    申请号：US16072825

    申请日：2017-03-16

    Applicant: The Trustees of Columbia University in the City of New York

    Inventor： Liang Liu ,  Yao Shen

    IPC: C12Q1/6886

    Abstract: The present invention provides methods of detecting ultraviolet radiation (UVR)-induced skin damage in a subject. The method includes the steps of: a) obtaining a skin sample from the subject; b) analyzing expression levels in the skin sample of UVR-induced differentially expressed genes (DEGs) listed in Table 8 or a subset thereof; and c) comparing the expression levels of the UVR-induced DEGs to a control skin sample; wherein, when the expression levels of the UVR-induced DEGs in the skin sample is above or below the level of each of the UVR-induced DEGs in the control sample, the subject is identified as likely being afflicted with UVR-induced skin damage. Also provided are methods for measuring the effectiveness of a test agent in reducing ultraviolet radiation (UVR)-induced damage.

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