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公开(公告)号:US20210199641A1
公开(公告)日:2021-07-01
申请号:US17156079
申请日:2021-01-22
发明人: Jingyue Ju , James J. Russo , Sergey Kalachikov , Chuanjuan Tao , Shiv Kumar , Irina Morozova , Xiaoxu Li
IPC分类号: G01N33/487 , G01N33/68
摘要: The present application discloses novel and inventive methods, apparatuses, and the required molecules to detect compounds (analytes) utilizing antibodies or other protein-binding molecules and nanopore-detectable tags using specially designed nanopores, coupled with ionic current readout, to provide a single molecule electronic detection solution, offering unique opportunities for multiplexing and quantitation.
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公开(公告)号:US20190112650A1
公开(公告)日:2019-04-18
申请号:US16091435
申请日:2017-04-04
发明人: Jingyue Ju , Shiv Kumar , James J. Russo , Steffen Jockusch , Zengmin Li , Xiaoxu Li , Sergey Kalachikov , Irina Morozova
IPC分类号: C12Q1/6869 , C12N9/12 , B82Y30/00 , C12Q1/6825 , G01N21/65 , B82Y5/00
摘要: This invention provides nucleoside polyphosphate analogues each of which comprises a tag comprising a plurality of Raman-scattering moieties; compounds comprising said nucleoside polyphosphate analogs. This invention also provides nucleotide polymerases with one or more attached and/or conjugated noble metal nanoparticles, wherein the noble metal nanoparticles are surface-enhanced Raman spectroscopy (SERS) substrates thereby creating a region of enhanced sensitivity for surface enhanced Raman spectroscopy (SERS) within or adjacent to the polymerase. This invention also provides a surface with regions of enhanced sensitivity for surface enhanced Raman spectroscopy comprising interspersed rough or nanostructured noble metal surface. This invention also provides methods for determining the sequence of a single stranded DNA or RNA polynucleotide using one or more of nucleoside polyphosphate analogues, polymerase with noble metal nanoparticles, and surface with noble metal.
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公开(公告)号:US20230028388A1
公开(公告)日:2023-01-26
申请号:US17816555
申请日:2022-08-01
发明人: Jingyue Ju , Xiaoxu Li , Xin Chen , Zengmin Li , Shiv Kumar , Shundi Shi , Cheng Guo , Jianyi Ren , Min-Kang Hsieh , Minchen Chien , Chuanjuan Tao , Ece Erturk , Sergey Kalachikov , James J. Russo
IPC分类号: C12Q1/6869 , C07H19/10 , C07H19/20 , C07H19/14
摘要: Disclosed herein, inter alia, are compounds, compositions, and methods of use thereof in the sequencing of a nucleic acid.
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4.
公开(公告)号:US20210381043A1
公开(公告)日:2021-12-09
申请号:US17405341
申请日:2021-08-18
发明人: Jingyue Ju , Dae Hyun Kim , Lanrong Bi , Qinglin Meng , Xiaoxu Li
IPC分类号: C12Q1/6869 , C12Q1/6823
摘要: This invention provides a process for sequencing single-stranded DNA by employing a nanopore and modified nucleotides.
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公开(公告)号:US20220372061A1
公开(公告)日:2022-11-24
申请号:US17622214
申请日:2020-07-09
发明人: Jingyue Ju , Shiv Kumar , James Russo , Xiaoxu Li , Xin Chen , Minchen Chien , Steffen Jockusch , Xuanting Wang , Irina Morozova , Chuanjuan Tao , Sergey Kalachikov , Shundi Shi
IPC分类号: C07H19/10 , C07H19/20 , C12Q1/6874 , C12Q1/6816
摘要: The invention provides various orthogonal nucleotide analogues and methods for using combinations of said various orthogonal nucleotide analogues for sequencing by synthesis.
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公开(公告)号:US20220364167A1
公开(公告)日:2022-11-17
申请号:US17860036
申请日:2022-07-07
发明人: Jingyue Ju , Shiv Kumar , James J. Russo , Steffen Jockusch , Zengmin Li , Xiaoxu Li , Sergey M. Kalachikov , Irina Morozova
IPC分类号: C12Q1/6869 , C07H19/10 , C07H19/20
摘要: This invention provides nucleotide analogues each of which comprises a tag comprising one or more Forster resonance energy transfer (FRET) acceptor fluorophores, a nucleotide polymerase having one or more FRET donor fluorophores, and methods for sequencing single-stranded
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7.
公开(公告)号:US20200208211A1
公开(公告)日:2020-07-02
申请号:US16722702
申请日:2019-12-20
发明人: Jingyue Ju , Dae Hyun Kim , Lanrong Bi , Qinglin Meng , Xiaoxu Li
IPC分类号: C12Q1/6869 , C12Q1/6823
摘要: This invention provides a process for sequencing single-stranded DNA employing modified nucleotides.
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公开(公告)号:US20200069717A1
公开(公告)日:2020-03-05
申请号:US16303715
申请日:2017-05-23
发明人: Jingyue Ju , Xin Chen , Xiaoxu Li , Zengmin Li , Min-Kang Hsieh , Minchen Chien , Shundi Shi , Jianyi Ren , Cheng Guo , Shiv Kumar , James J. Russo , Chuanjuan Tao , Steffen Jockusch , Sergey Kalachikov
IPC分类号: A61K31/7052 , C07H21/04
摘要: Disclosed herein, inter alia, are compounds, compositions, and methods of thereof in the sequencing a nucleic acid.
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9.
公开(公告)号:US20190153527A1
公开(公告)日:2019-05-23
申请号:US16091442
申请日:2017-04-04
发明人: Jingyue Ju , Shiv Kumar , James J. Russo , Steffen Jockusch , Zengmin Li , Xiaoxu Li , Sergey M. Kalachikov , Irina Morozova
IPC分类号: C12Q1/6869 , C07H19/10 , C07H19/20
摘要: This invention provides nucleotide analogues each of which comprises a tag comprising one or more Forster resonance energy transfer (FRET) acceptor fluorophores, a nucleotide polymerase having one or more FRET donor fluorophores, and methods for sequencing single-stranded
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公开(公告)号:US11266673B2
公开(公告)日:2022-03-08
申请号:US16303715
申请日:2017-05-23
发明人: Jingyue Ju , Xin Chen , Xiaoxu Li , Zengmin Li , Min-Kang Hsieh , Minchen Chien , Shundi Shi , Jianyi Ren , Cheng Guo , Shiv Kumar , James J. Russo , Chuanjuan Tao , Steffen Jockusch , Sergey Kalachikov
IPC分类号: C12Q1/68 , A61K31/7052 , C07H21/04 , C07H19/20 , C12Q1/6869
摘要: Disclosed herein, inter alia, are compounds, compositions, and methods of use thereof in the sequencing a nucleic acid.
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