公开(公告)号：US20110060837A1

公开(公告)日：2011-03-10

申请号：US12933726

申请日：2008-06-20

申请人： Hengxing Zhai ,  Lin Liu

发明人： Hengxing Zhai ,  Lin Liu

IPC分类号： G06F15/16

CPC分类号： H04W76/10 ,  H04W92/045

摘要： A method, system and device for establishing connection based on a LTE/SAE system. First, e-NodeB sends an S1 SETUP REQUEST message to the MME, to initialize the establishment of the S1 connection; then, MME responds an S1 SETUP RESPONSE message to the e-NodeB, in response to the S1 SETUP REQUEST message, carrying the MME identification in the S1 SETUP RESPONSE message.

摘要翻译： 一种基于LTE / SAE系统建立连接的方法，系统和装置。 首先，e节点B向MME发送S1 SETUP REQUEST消息，初始化S1连接的建立; 那么MME响应于在S1 SETUP RESPONSE消息中携带MME标识的S1 SETUP REQUEST消息，向e-NodeB响应S1 SETUP RESPONSE消息。

公开(公告)号：US20090087910A1

公开(公告)日：2009-04-02

申请号：US12192356

申请日：2008-08-15

申请人： Lin Liu ,  Deming Gou

发明人： Lin Liu ,  Deming Gou

IPC分类号： C12N15/87 ,  C12P19/34

CPC分类号： C12N15/111 ,  C12N15/10 ,  C12N15/64 ,  C12N15/66 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53 ,  C12N2330/30

摘要： Functional shRNA is produced from an expression vector prepared by selecting a two primer design in which the primers are less than about 50 nucleotides in length, annealing and extending the primers using primer extension, digesting the primer extension product and inserting the digestion product into a suitable vector. When the shRNA vectors are inserted into a cell, shRNA transcribed from the vectors modulates gene activity within the cell.

摘要翻译： 从通过选择引物小于约50个核苷酸长度的两个引物设计制备的表达载体产生功能性shRNA，使用引物延伸退火并延伸引物，消化引物延伸产物并将消化产物插入合适的 向量。 当将shRNA载体插入细胞时，从载体转录的shRNA调节细胞内的基因活性。

公开(公告)号：US07387893B2

公开(公告)日：2008-06-17

申请号：US10914529

申请日：2004-08-09

申请人： David Martin Anderson ,  Lin Liu ,  Sergey Podkovyrov ,  Baomin Wang

发明人： David Martin Anderson ,  Lin Liu ,  Sergey Podkovyrov ,  Baomin Wang

IPC分类号： C12N15/00 ,  C12N1/20 ,  C12N5/10 ,  C07H21/00 ,  C12N9/02 ,  C12N9/12 ,  C12N9/78 ,  C12Q1/26 ,  C12P21/06 ,  C07K14/00

CPC分类号： C12N9/0036 ,  C12N9/0093 ,  C12N9/1007 ,  C12N9/1205 ,  C12N9/78 ,  C12N15/52 ,  C12P19/385

摘要： Novel DNA constructs and host cells comprising the same are disclosed. DNA constructs comprise a transcription unit (e.g. operon) comprising DNA sequences encoding for ribonucleotide reductase and thioredoxin or a uridine kinase gene and/or a dCTP deaminase gene. In preferred embodiments the constructs comprising DNA sequences encoding for ribonucleotide reductase and thioredoxin further comprise DNA sequences encoding for thymidylate synthase and/or transcription units comprising sequences encoding for uridine kinase preferably together with dCTP deaminase. In particularly preferred embodiments, the host cells comprise constructs having all of the above characteristics wherein the host cell displays repressed or no uracil DNA glycosylase activity. This may be achieved by removal of the host cell ung gene. Use of host cells in the manufacture of pyrimidine deoxyribonucleotides e.g. thymidine is also disclosed.

摘要翻译： 公开了新的DNA构建体和包含其的宿主细胞。 DNA构建体包含包含编码核糖核苷酸还原酶和硫氧还蛋白或尿苷激酶基因和/或dCTP脱氨酶基因的DNA序列的转录单位（例如操纵子）。 在优选实施方案中，包含编码核糖核苷酸还原酶和硫氧还蛋白的DNA序列的构建体还包含编码胸苷酸合酶的DNA序列和/或包含编码尿苷激酶的序列的转录单元，优选与dCTP脱氨酶一起。 在特别优选的实施方案中，宿主细胞包含具有所有上述特征的构建体，其中宿主细胞显示被抑制或没有尿嘧啶DNA糖基化酶活性。 这可以通过去除宿主细胞ung基因来实现。 宿主细胞在制造嘧啶脱氧核糖核苷酸中的用途，例如 还公开了胸苷。

公开(公告)号：US06780628B2

公开(公告)日：2004-08-24

申请号：US09731971

申请日：2000-12-08

申请人： David M. Anderson ,  Lin Liu ,  Humg-Yu Hsiao ,  Douglas W. Fodge

发明人： David M. Anderson ,  Lin Liu ,  Humg-Yu Hsiao ,  Douglas W. Fodge

IPC分类号： A23L131

CPC分类号： A61K38/47 ,  A23K20/189 ,  A61K38/465 ,  C12Y301/04011 ,  C12Y302/01078

摘要： Enzymes of a particular class, characterized by the ability to cleave a linkage that effects release of a cell-surface protein or carbohydrate, which does not contain an anti-infection agent, display significant anti-infectious activity. Upon oral administration, these enzymes are effective, for example, in the treatment of digestive tract infections in humans and in animals. In the latter, there are benefits of significantly improved growth rate, feed efficiency, and overall health.

公开(公告)号：US06777209B1

公开(公告)日：2004-08-17

申请号：US10018878

申请日：2002-03-06

申请人： David Martin Anderson ,  Lin Liu ,  Sergey Podkovyrov ,  Baomin Wang

发明人： David Martin Anderson ,  Lin Liu ,  Sergey Podkovyrov ,  Baomin Wang

IPC分类号： C12N120

CPC分类号： C12N9/0036 ,  C12N9/0093 ,  C12N9/1007 ,  C12N9/1205 ,  C12N9/78 ,  C12N15/52 ,  C12P19/385

摘要： Novel DNA constructs and host cells comprising the same are disclosed. DNA constructs comprise a transcription unit (e.g. operon) comprising DNA sequences encoding for ribonucleotide reductase and thioredoxin or a uridine kinase gene and/or a dCTP deaminase gene. In preferred embodiments the constructs comprising DNA sequences encoding for ribonucleotide reductase and thioredoxin further comprise DNA sequences encoding for thymidylate synthase and/or transcription units comprising sequences encoding for uridine kinase preferably together with dCTP deaminase. In particularly preferred embodiments, the host cells comprise constructs having all of the above characteristics wherein the host cell displays repressed or no uracil DNA glycosylase activity. This may be achieved by removal of the host cell ung gene. Use of host cells in the manufacture of pyrimidine deoxyribonucleotides e.g. thymidine is also disclosed.

摘要翻译： 公开了新的DNA构建体和包含其的宿主细胞。 DNA构建体包含包含编码核糖核苷酸还原酶和硫氧还蛋白或尿苷激酶基因和/或dCTP脱氨酶基因的DNA序列的转录单位（例如操纵子）。 在优选实施方案中，包含编码核糖核苷酸还原酶和硫氧还蛋白的DNA序列的构建体还包含编码胸苷酸合酶的DNA序列和/或包含编码尿苷激酶的序列的转录单元，优选与dCTP脱氨酶一起。 在特别优选的实施方案中，宿主细胞包含具有所有上述特征的构建体，其中宿主细胞显示被抑制或没有尿嘧啶DNA糖基化酶活性。 这可以通过去除宿主细胞ung基因来实现。 宿主细胞在制造嘧啶脱氧核糖核苷酸中的用途，例如 还公开了胸苷。